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de novo Protein Design
de novo Protein Design

... Search of nearby conformational space and sequence space 2 methods of protein redesign (variation of backbone conformation and amino acid sequence) Development of procedure for identifying low free energy sequence-structure pairs that iterates between sequence optimization and structure prediction R ...
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“Building” proteins!!

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MOLECULAR BIOLOGY EXAM II

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Biology Pre-Learning Check
Biology Pre-Learning Check

... In this short unit, we will be studying the RNA molecule in detail, comparing it to what we learned about DNA. We will look at the specific structure of the subunits (nucleotides) as well as the structure of the RNA molecule itself and comparing/contrasting it with DNA We will also study the process ...
Covering the distance between discovery and treatment must be a
Covering the distance between discovery and treatment must be a

... Covering the distance between discovery and treatment is a team effort No one research group can do it alone ...
What meaning(s) do these two photos represent? (Hint* dna,rna
What meaning(s) do these two photos represent? (Hint* dna,rna

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Nonstandard amino acids are found in modified proteins

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Amino acids have many roles in living organisms
Amino acids have many roles in living organisms

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Basics of Molecular Biology
Basics of Molecular Biology

... https://ocw.mit.edu/courses/biology/7-343-network-medicine-using-systems-biology-and-signaling- ...
Gene Ontology (GO)
Gene Ontology (GO)

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Protein synthesis and Enzyme test review

... 18. List the 3 parts of the RNA nucleotide. = Sugar (ribose), phosphate, nitrogen base (A-U, C-G) 19. What is transcription? Copying DNA into mRNA takes place in the nucleus 20. What is translation? mRNA goes to the ribosomes – sends a message to tRNA to go get the amino acids to build a protein 21. ...
BIO 6.3 Carbon - Steinbach Science
BIO 6.3 Carbon - Steinbach Science

... Lipids  are  organic  compounds  that  have  a  large   portion  (much  greater  than  2  to  1)  or  C—H   bonds  and  less  oxygen  than  carbohydrates  (e.g.,   beef  fat  has  the  formula  C57H110O6)   Lipids  are  commonly  call ...
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LECT09 fibro

... bond covalently to either N or O is attracted by an electron pair from a neighboring N or O. The attracting force is basically electrostatic. Disulfide Bond: A strong covalent bond formed by two –SH groups of cysteines. This bond can only be broken to component -SH groups by reducing agents. Electro ...
What is latency? - California State University, Fullerton
What is latency? - California State University, Fullerton

... • Combinatorial chemistry with good screen test – can do 50000 compounds a day – Chemical libraries for big firms – any drug with some reaction can be modified – Screening assays • Preventing replication • Transcriptional regulation - luciferase expression • Protease inhibitor - modify tetracycline ...
Lectures on Computational Biology
Lectures on Computational Biology

How to classify proteins on basis of structure?
How to classify proteins on basis of structure?

... information of a protein is necessary to explain and predict its gene function as well as to design molecules that bind to the protein in drug design. • Today, whole genome sequences (the complete set of genes) of various organisms have been deciphered and we realize that functions of many genes are ...
Proteins Large, complex polymer consists of carbon, oxygen
Proteins Large, complex polymer consists of carbon, oxygen

... hydrogen, nitrogen and sometimes sulfur. It is the most varied  of the carbon­based molecules. In movement, eyesight and digestion proteins are at work. ­ are essential to all life ­provide structure for tissues and organs and carries out  cell metabolism ­ Come in a variety of shapes and sizes ...
Organic Biomolecules Fill in Notes 2016
Organic Biomolecules Fill in Notes 2016

... After amino acids are linked together, the chain folds into a specific shape! Shape determines protein’s functions! ...
PowerPoint- Protein Shape
PowerPoint- Protein Shape

... 3) What are the steps of protein synthesis? 4) Compare transcription and translation. 5) How is DNA different from mRNA? HW: 1) Daily Review of class notes. 2) Textbook worksheet due Friday ...
Nutrients
Nutrients

... Ruminants vs Non-Runinants Protein Ruminants can make essential amino acids ...
04b Carbohydrates-student note
04b Carbohydrates-student note

Molecular Biology Final Exam (Set A)
Molecular Biology Final Exam (Set A)

... nitrogenous bases would be exposed to the water solvent. This is unfavorable, since the bases are largely hydrophobic. Instead, RNA folds up on itself, forming internal basepairs wherever its sequence allows. Since this internal basepairing relies on self-complementary sequence, the way in which an ...
some molecular basics
some molecular basics

... can associate with specific codon combinations in the mRNA ...
Slide 1
Slide 1

... substitution (i.e., non-synonymous SNPs) on protein structure and function based on: – Amino acid sequence • What part of the protein did the SNP occur? (E.g., active site, binding site, transmembrane region) ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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