• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
lecture5lifes_chemical_basis
lecture5lifes_chemical_basis

... The elucidation of the structure of α-helix is a landmark in Biochemistry because it was demonstrated that the conformation of a polypeptide chain can be predicted if the properties of its constituents are rigorously and precisely known. For this work Pauling got the Nobel prize in Chemistry in 1954 ...
8. DNA,RNA Membranes, Cytoskeleton
8. DNA,RNA Membranes, Cytoskeleton

... DNA REPLICATION: enables a complete copy of the genome to be passed on to each daughter cell during mitosis TRANSCRIPTION OF DNA into RNA: mechanism by which genes are expressed ...
Protein structure visualization and analysis
Protein structure visualization and analysis

Word of the Day
Word of the Day

... RNA polymerase unzips DNA and copies it into RNA. A’s connect with U’s and G’s connect with C’s. The starting point of transcription is known as the Promoter, the end is known as the terminal signal. After transcription the mRNA moves into the cytosol for protein synthesis. ...
lecture 5
lecture 5

... Figure 2. Overall Structure of PDI (A) Ribbon diagram of PDI with the a, b, b′, and a′ domains in magenta, cyan, yellow, and red, respectively, and the C-terminal extension in green. The two orientations roughly differ by a 90° rotation around the horizontal axis. The side chains of the active site ...
Bonding is more than attraction
Bonding is more than attraction

... hydrogen, and oxygen atoms in the proportion of 1:2:1. ...
Abstract
Abstract

... can potentially be used as a scaffold to create a targeted drug delivery system. It is capable of assembling into a 60-mer unit dodecahedron with icosahedral symmetry, even with the addition of peptides on the surface. Circular dichroism (CD) and differential scanning calorimetry (DSC) were used to ...
Questions Ch 24
Questions Ch 24

... 3) Ampicillin is a semi-synthetic antibiotic derivative of penicillin G. Ampicillin is acidresistant, whereas penicillin G is not resistant to acid. Why is this important? a) Ampicillin is resistant to β-lactamases. b) Ampicillin can be taken orally. c) Ampicillin is effective against Gram-negative ...
Lecture Slides - METU Computer Engineering
Lecture Slides - METU Computer Engineering

... • Prediction remains a challenge – ab-initio (energy minimization) – knowledge-based • Chou-Fasman and GOR methods for SSE prediction • Comparative modeling and protein threading for tertiary structure prediction ...
Human Proteome advertising miniposter (PDF)
Human Proteome advertising miniposter (PDF)

... Antibodypedia is a web-based knowledge resource with annotated and scored antibodies from commercial and academic providers. All information is free and accessible in the database. With the knowledge in Antibodypedia you have the power to select the right antibody for the right application. ...
Lecture Slides
Lecture Slides

... • Prediction remains a challenge – ab-initio (energy minimization) – knowledge-based • Chou-Fasman and GOR methods for SSE prediction • Comparative modeling and protein threading for tertiary structure prediction ...
Integral proteins
Integral proteins

... Integral proteins have domains that extend into the hydrocarbon core of the membrane. Often they span the bilayer. Intramembrane domains have largely hydrophobic surfaces, that interact with membrane lipids Amphipathic detergents are required for solubilization of integral proteins from membranes  ...
Supplementary Information
Supplementary Information

No Slide Title
No Slide Title

... There are about >20,000 structures in PDB, and about 1 million protein sequences in SwissProt/ TrEMBL. For most proteins, structural models derive from computational biology approaches, rather than experimental methods. The most reliable method of modeling and evaluating new structures is by compari ...
ppt part 1 - Embrace Challenge
ppt part 1 - Embrace Challenge

... Procedures ...
Lecture_11_2005
Lecture_11_2005

... • FtsZ and Tubulin have limited sequence similarity and would not be identified as homologous proteins by sequence analysis. ...
1 Supplementary data Materials and methods Preparation of the
1 Supplementary data Materials and methods Preparation of the

... Supplementary references Chuang, J.L., Wynn, R.M. and Chuang, D.T. (2002) The C-terminal hinge region of lipoic acidbearing domain of E2b is essential for domain interaction with branched-chain alpha-keto acid dehydrogenase kinase. J Biol Chem, 277, 36905-36908. Collaborative Computational Project, ...
Coffee: More Than Just a Jolt in the Morning
Coffee: More Than Just a Jolt in the Morning

... Another noteworthy plant protein is found in nuts(4). Nuts have been found to be particularly beneficial to one’s health. Nuts lower triglycerides, trigger apoptosis (cell suicide) in cancer cells, and are a good source of vitamin E, a powerful anti-oxidant(4). Some proponents of plant protein note ...
on February 28, 2008 Downloaded from www.sciencemag.org
on February 28, 2008 Downloaded from www.sciencemag.org

... homology among ZFPJS, ttk, Br-c, PLZF, and BCL-6 also share homology with viral proteins (for example, VA55R) of the poxvirus family (23) as well as with the Drosophila kelch protein involved in nurse celloocyte interaction (24). These structural homologies suggest that BCL-6 may function as a DNA b ...
Leukaemia Section t(3;11)(q25;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(3;11)(q25;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Online updated version : http://AtlasGeneticsOncology.org/Anomalies/t311GMPSID1173.html DOI: 10.4267/2042/37600 This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2000 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Protein basics - Crop Genebank Knowledge Base
Protein basics - Crop Genebank Knowledge Base

Ch. 17 - Ltcconline.net
Ch. 17 - Ltcconline.net

... A. DNA genotype is expressed as proteins 1. genes specify proteins 2. proteins – structure/function of bodies. 3. review classes of proteins? 4. DNA specifies the synthesis of proteins thru 2 major steps = transcription and translation 5. Fig. 10.6a. DNA ______>RNA_______> proteins transcription tra ...
Protein Structure and Function
Protein Structure and Function

... Motifs are regular combinations of secondary structures. Motifs form domains! ...
Building Materials of Life
Building Materials of Life

Document
Document

... Every different polypeptide has a different primary structure (sequence). By definiton. The migration behavior of each sub-peptide depends on its composite properties. The properties are suffiently complex such that most subpeptides in a given polypeptide will behave differently. Every polypeptide w ...
< 1 ... 421 422 423 424 425 426 427 428 429 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report