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Prediction - Center for Biological Sequence Analysis
Prediction - Center for Biological Sequence Analysis

... ”neighbours” (too closely related) • Calculate numbers of neighbours for each example, and remove the example with most neighbours • Repeat until there are no examples with neighbours left Alternative: Homology partitioning • keep all examples, but cluster them so that no neighbours end up in the sa ...
PowerPoint
PowerPoint

... general lack of sequence homology, the ancestry of this molecule is still a mystery. ...
Todd Eckdahl - Davidson College
Todd Eckdahl - Davidson College

... Minor Groove Binding Drugs  Anti-tumor properties  Conformational change in the 3D structure of DNA  Prior Knowledge of MGBD/DNA interaction  As models for minor groove binding proteins ...
Protein Translation
Protein Translation

... Linear: uses mRNA which is complementary to DNA sequence. Triplet: the unit of information is the codon, a series of three ribonucleotides. Unambiguous: each codon specifies only one amino acid (AA). Degenerate: more than one codon exists for most amino acids. ...
ReviewExamIII
ReviewExamIII

... Define the terms we used in the Enzyme lab (such as substrate, competitive inhibitor, active site, noncompetitive inhibitor, and catalysis) and review the exact chemical reaction (reactants and products) that we studied in the Enzyme lab. How does pH and temperature affect enzymatic rate (velocity)? ...
Signal Transduction
Signal Transduction

... membrane surface by binding to products of PI-3 Kinase, e.g., PI-3,4,5-P3.  Other kinases at the cytosolic surface of the plasma membrane then catalyze phosphorylation of Protein Kinase B, activating it.  Activated Protein Kinase B catalyzes phosphorylation of Ser or Thr residues of many proteins, ...
Molecular Evolution
Molecular Evolution

... • The differences in the rates of evolution are usually due to functional constraints •  mutations that remove or reduce the function of a gene are removed by negative selection •  very important genes tend to evolve slowly •  proteins (gene products) that interact with other proteins etc. also evo ...
Protein structure - Wikispaces
Protein structure - Wikispaces

... Proteins which fold into a ball or ‘globule’ like Myoglobin are called Globular Proteins. They tend to be soluble. The most common group of Globular Proteins are ENZYMES which control the reactions in ...
Bacterial Bioreactors for High Yield Production of Recombinant Protein
Bacterial Bioreactors for High Yield Production of Recombinant Protein

ShowTec Mintrate - Solon Feed Mill
ShowTec Mintrate - Solon Feed Mill

... ShowTec Swine Mintrate products are non-medicated protein-vitamin-mineral supplements formulated to be mixed with soybean meal and grain to make complete show pig feeds. Complete rations provide specific amounts and ratios of nutrients needed to enable show pigs to make fast, efficient, lean gain. S ...
Chapter 6 Section 3
Chapter 6 Section 3

... ex: fats, oils, waxes and steroids They are insoluble in water Imp. For proper organism function Explanation of SATURATED and UNSATURATED fats—pg 160 ...
Awan, Ali: In Silico Transfer of Ligand Binding Function between Structurally Analogous Proteins
Awan, Ali: In Silico Transfer of Ligand Binding Function between Structurally Analogous Proteins

... protein modification has been used to alter protein performance for industrial purposes. It has been possible to change the rates, temperatures and pHs at which enzymes operate, to increase efficiency and applicability. Further, it has become possible to modify not only protein performance, but even ...
PROTEIN
PROTEIN

... Protein Metabolic Waste Product ---> Urinary Nitrogen : urea and non protein nitrogen (creatinin and uric acid) ...
Biochemistry Objective Sheet Test Objectives Bio.1.2.1 • Explain
Biochemistry Objective Sheet Test Objectives Bio.1.2.1 • Explain

... Name several examples of each of the four major organic compounds. Identify the chemical indicators used to identify the presence of organic compounds. Explain that proteins differ in their sequence of amino acids and that this sequence determines the structure of the protein, and thus, its function ...
The Structure and Function of Large Biological Molecules
The Structure and Function of Large Biological Molecules

... – 20 different amino acids that are composed of an asymmetric carbon surrounded by an amino group, carboxyl group, hydrogen and an R group or side chain which varies ...
Anton Supercomputer, a computational microscope.
Anton Supercomputer, a computational microscope.

... Determined for each protein how many folding pathways are traversed that are distinct in the sense that native interactions are formed in different orders and that the pathways do not interconvert on the transition path time scale. Examined the thermodynamics and kinetics of the folding process, and ...
Review on Biochemistry: Protein Chemistry
Review on Biochemistry: Protein Chemistry

... Inhibition can not be reversed by substrate. Uncompetitive I binds only to ES complex other than the active site. Inhibition can not be reversed by substrate.  Competitive:  Methanol vs. ethanol and alcohol dehydrogenase  CO vs. O2 ...
Updated - PeproTech Posters
Updated - PeproTech Posters

... proliferation, p53-induced TIGAR expression prevents outgrowth of cells harboring damaged DNA. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recomb ...
Introduction to molecular biology
Introduction to molecular biology

... Cell functions: A cell contains all the necessary information to perform a replication (a virus does not!). Processes developed by cells include: Metabolic pathways Traduction of RNA to proteins ...
Unknown function, JCSG
Unknown function, JCSG

... JCSG is improving the structural coverage by determining novel structures which share <30% sequence identity with those in the PDB. Hidden Markov Models from the CATH database were used to identify sequences in the JCSG genome pool. PSI-Blast seeded with these sequences was used to find additional p ...
Slide 1 - MacWilliams Biology
Slide 1 - MacWilliams Biology

... 1. First step in decoding genetic messages  transcribe a nucleotide base sequence from DNA to RNA. 2. Transcribed information contains a code for making proteins. 3. Proteins are made by joining amino acids together into long chains, called polypeptides. 4. As many as 20 different amino acids are c ...
Document
Document

lecture 47 slides no animations
lecture 47 slides no animations

Modeling with Toobers
Modeling with Toobers

Supplemental Information
Supplemental Information

... Description and summary of the proteins identified in the comparative proteome analysis of vehicle- and endorepellin-treated human endothelial cells. The following table represents the 106 proteins identified, categorized according to the functional designations used in Figure 1. A brief summary of ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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