Two-Metal-Ion Catalysis in Adenylyl Cyclase
... structure, -L-2⬘,3⬘-dd-5⬘-ATP appears to bind as a complex with two metal ions. The first metal (metal A) is coordinated by the pro-R oxygen of the ␣ phosphate, a carboxylate oxygen from each of the invariant aspartic acid residues [Asp396 (D396) and D440], and a water molecule. The four ligands fo ...
... structure, -L-2⬘,3⬘-dd-5⬘-ATP appears to bind as a complex with two metal ions. The first metal (metal A) is coordinated by the pro-R oxygen of the ␣ phosphate, a carboxylate oxygen from each of the invariant aspartic acid residues [Asp396 (D396) and D440], and a water molecule. The four ligands fo ...
No Slide Title
... In theory: same sequence could code for 2 or 3 different polypeptides Most DNA sequences: one reading frame; one protein product ...
... In theory: same sequence could code for 2 or 3 different polypeptides Most DNA sequences: one reading frame; one protein product ...
E. coli
... the production of a medically important protein using recombinant DNA technology. You are provided with a series of cards. These begin with a general introduction (cards 2-3) and the outline of the characteristics of the three particular proteins (cards 4-6). After choosing which protein you want to ...
... the production of a medically important protein using recombinant DNA technology. You are provided with a series of cards. These begin with a general introduction (cards 2-3) and the outline of the characteristics of the three particular proteins (cards 4-6). After choosing which protein you want to ...
Functions of the Rumen
... • Disorder of metabolism – Insufficient energy intake in high producing animals (e.g. Dairy cattle in early lactation and sheep in late pregnancy) – Results in catabolism (breakdown) of body energy (fat) reserves ...
... • Disorder of metabolism – Insufficient energy intake in high producing animals (e.g. Dairy cattle in early lactation and sheep in late pregnancy) – Results in catabolism (breakdown) of body energy (fat) reserves ...
Norton J Nutr 2006
... and inhibiting Rheb. Because AMPK is activated by exercise and a decreased ATP/AMP ratio, AMPK activation and its effects on the mTOR pathway appear to be a primary mechanism reducing protein synthesis after endurance exercise (30). Leucine and mTOR-independent mechanisms Although important, mTOR al ...
... and inhibiting Rheb. Because AMPK is activated by exercise and a decreased ATP/AMP ratio, AMPK activation and its effects on the mTOR pathway appear to be a primary mechanism reducing protein synthesis after endurance exercise (30). Leucine and mTOR-independent mechanisms Although important, mTOR al ...
Document
... 1. The standard IUPAC one-letter codes for the amino acids are used. 2. The symbol `x' is used for a position where any amino acid is accepted. 3. Ambiguities are indicated by listing the acceptable amino acids for a given position, between square parentheses `[ ]'. For example: [ALT] stands for Ala ...
... 1. The standard IUPAC one-letter codes for the amino acids are used. 2. The symbol `x' is used for a position where any amino acid is accepted. 3. Ambiguities are indicated by listing the acceptable amino acids for a given position, between square parentheses `[ ]'. For example: [ALT] stands for Ala ...
Powerpoint slides
... a common ancestor (i.e. they are homologous). • … or, perhaps two proteins may be similar because they perform similar functions and are thereby constrained, even though they arose independently (functional convergence hypothesis, they are then called analogous). ...
... a common ancestor (i.e. they are homologous). • … or, perhaps two proteins may be similar because they perform similar functions and are thereby constrained, even though they arose independently (functional convergence hypothesis, they are then called analogous). ...
Proti-Ace Kit - Hampton Research
... A proteolytic fragment or domain of a protein may crystallize more readily or form better diffracting crystals than the intact protein.1-8 Proteases can be used to generate small, active fragments or domains of the target protein for crystallization.9 The fragment or domain can be used directly for ...
... A proteolytic fragment or domain of a protein may crystallize more readily or form better diffracting crystals than the intact protein.1-8 Proteases can be used to generate small, active fragments or domains of the target protein for crystallization.9 The fragment or domain can be used directly for ...
Features of the DNA Double Helix - E
... Disaccharides and polysaccharides must be ultimately broken down into monosaccharides in the digestive process known as hydrolysis. Only then can they be utilized by the body. Three monosaccharides are particularly important in the study of nutritional science: glucose, fructose and galactose. Gluco ...
... Disaccharides and polysaccharides must be ultimately broken down into monosaccharides in the digestive process known as hydrolysis. Only then can they be utilized by the body. Three monosaccharides are particularly important in the study of nutritional science: glucose, fructose and galactose. Gluco ...
The integrity of a cholesterol-binding pocket in Niemann–Pick C2
... known mechanism of its action prompted a genetic structure– function study of its properties. To this end, we purified wild-type and mutant NPC2 proteins, starting from a culture medium into which cells had released NPC2. Previous studies of NPC2 used NPC2-conditioned media (7) or NPC2 purified from ...
... known mechanism of its action prompted a genetic structure– function study of its properties. To this end, we purified wild-type and mutant NPC2 proteins, starting from a culture medium into which cells had released NPC2. Previous studies of NPC2 used NPC2-conditioned media (7) or NPC2 purified from ...
Improved Tolerance to Salt and Water Stress in
... EGTA, 0.1% BSA, 20 mM HEPES-KOH, pH 7.1), and cells were permeabilized by addition of digitonin dissolved in DMSO at 10 mg/ml (final concentration 6 µg · 10-6 cells). This concentration was chosen after careful titrations with digitonin and found to be sufficient to permeabilize the plasma membrane ...
... EGTA, 0.1% BSA, 20 mM HEPES-KOH, pH 7.1), and cells were permeabilized by addition of digitonin dissolved in DMSO at 10 mg/ml (final concentration 6 µg · 10-6 cells). This concentration was chosen after careful titrations with digitonin and found to be sufficient to permeabilize the plasma membrane ...
Genetic Biomarkers of Aging Drosophila Melanogaster Daria Solodovnikova
... with increased flying activity. As a result, appreciable changes in the expression of the following genes were observed: Impl3, Hsp22 and Hsp70. Impl3 expression increased with age in all the conditions studied, so it can be used as a biomarker of aging in all experimental strains. The expression of ...
... with increased flying activity. As a result, appreciable changes in the expression of the following genes were observed: Impl3, Hsp22 and Hsp70. Impl3 expression increased with age in all the conditions studied, so it can be used as a biomarker of aging in all experimental strains. The expression of ...
Amino Acids and Proteins - Gadarif University Repository
... linked together in varying sequences to form a vast variety of proteins. ...
... linked together in varying sequences to form a vast variety of proteins. ...
Sequence Entropy and the Absolute Rate of Amino Acid Substitutions
... specific stability contribution is small relative to the background contribution, so that this second term fulfills the role of the ‘thermal bath’ in statistical physics. This statistical mechanics formalism can now be applied to modeling the amino acid substitution rate. ...
... specific stability contribution is small relative to the background contribution, so that this second term fulfills the role of the ‘thermal bath’ in statistical physics. This statistical mechanics formalism can now be applied to modeling the amino acid substitution rate. ...
New concepts of cellular fatty acid uptake: role of fatty acid transport
... various proteins have been suggested to be involved in this process. Amongst these proteins is a family of membrane-associated proteins termed fatty acid transport proteins (FATP). So far six members of this family, designated FATP 1–6, have been characterized. FATP 1, 2 and 6 show a highly-conserve ...
... various proteins have been suggested to be involved in this process. Amongst these proteins is a family of membrane-associated proteins termed fatty acid transport proteins (FATP). So far six members of this family, designated FATP 1–6, have been characterized. FATP 1, 2 and 6 show a highly-conserve ...
the Acetyl-Coenzyme A
... an unusual context for a translational start site, while the next ATG. 24 codons downstream. is in a more conventional context. Possible implications of two alternative translational start sites for the cellular localization o r the enzyme are discussed. A stable mutant of this gene. obtained by the ...
... an unusual context for a translational start site, while the next ATG. 24 codons downstream. is in a more conventional context. Possible implications of two alternative translational start sites for the cellular localization o r the enzyme are discussed. A stable mutant of this gene. obtained by the ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... Hochschild and Ptashne, 1986). By analogy with repressor proteins, the Bicoid recognition helix has been predicted to encompass, minimally, residues 138-147 in the native protein. Here, these ten amino acids will be referred to as positions 1 through 10. To confirm that the mutant LexA-Bicoid protei ...
... Hochschild and Ptashne, 1986). By analogy with repressor proteins, the Bicoid recognition helix has been predicted to encompass, minimally, residues 138-147 in the native protein. Here, these ten amino acids will be referred to as positions 1 through 10. To confirm that the mutant LexA-Bicoid protei ...
word - My eCoach
... Cellulose and Chitin are polysaccharides that function to support and protect the organism. The cell walls of plants are composed of cellulose. The cell walls of fungi and the exoskeleton of arthropods are composed of chitin. Cellulose is composed of beta-glucose monomers; starch and glycogen are co ...
... Cellulose and Chitin are polysaccharides that function to support and protect the organism. The cell walls of plants are composed of cellulose. The cell walls of fungi and the exoskeleton of arthropods are composed of chitin. Cellulose is composed of beta-glucose monomers; starch and glycogen are co ...
Isolation and characterization of cDNA clones encoding
... nitrocellulose membranes. When these filters were probed with a PR-la specific polyclonal antiserum, a single 18 kD protein band was seen with extracts from the virus infected leaves (Fig. 1A, lane 1 ) , but not with mock inoculated control plants (Fig. 1A, lane 2 ) . The same plants were also used ...
... nitrocellulose membranes. When these filters were probed with a PR-la specific polyclonal antiserum, a single 18 kD protein band was seen with extracts from the virus infected leaves (Fig. 1A, lane 1 ) , but not with mock inoculated control plants (Fig. 1A, lane 2 ) . The same plants were also used ...
manualE6901
... E. coli strain ER2566 ER2566 is provided as a host strain for the expression of a target gene cloned into the IMPACT vectors. ER2566 carries a chromosomal copy of the T7 RNA polymerase gene inserted into the lacZ gene, and thus is under the control of the lac promoter. The strain is deficient in bo ...
... E. coli strain ER2566 ER2566 is provided as a host strain for the expression of a target gene cloned into the IMPACT vectors. ER2566 carries a chromosomal copy of the T7 RNA polymerase gene inserted into the lacZ gene, and thus is under the control of the lac promoter. The strain is deficient in bo ...
Biosensor-based engineering of biosynthetic pathways
... factors (aTFs) change their affinity for an operator DNA sequence through a conformational change enacted by ligand binding and are useful for directly controlling gene transcription [21]. Ligand-dependent protein dimerization [22] and ligand-conditional protein stability are related methods that re ...
... factors (aTFs) change their affinity for an operator DNA sequence through a conformational change enacted by ligand binding and are useful for directly controlling gene transcription [21]. Ligand-dependent protein dimerization [22] and ligand-conditional protein stability are related methods that re ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.