NCBI: what is new? - medicalintelligence.org
... • Perform research into advanced methods of analyzing and interpreting molecular biology data. • Enable biotechnology researchers and medical care personnel to use the systems and methods developed. ...
... • Perform research into advanced methods of analyzing and interpreting molecular biology data. • Enable biotechnology researchers and medical care personnel to use the systems and methods developed. ...
Summer 2003 Test 3
... 30) Within the structure of a vertebrate neuron, open non-myelinated areas are referred to as? a) Schwann cells b) internuncial neurons c) axons d) Nodes of Ranvier e) none of these 31) The resting potential of the squid giant axon is NOT due in part or whole to which of the following? a) active tra ...
... 30) Within the structure of a vertebrate neuron, open non-myelinated areas are referred to as? a) Schwann cells b) internuncial neurons c) axons d) Nodes of Ranvier e) none of these 31) The resting potential of the squid giant axon is NOT due in part or whole to which of the following? a) active tra ...
Document
... group gives a complex titration curve with two inflection points. 3. More complex amino acids with an ionizable R group show even more complex titration curves. ...
... group gives a complex titration curve with two inflection points. 3. More complex amino acids with an ionizable R group show even more complex titration curves. ...
Regulation of transcript encoding the 43K
... purified phage from 24 plaques and analysed DNA from these phage by Southern blots. Phage DNA was digested separately with EcoRl and Stul and Southern blots were probed with 32P-T43k.l cDNA. Based upon the restriction maps of cDNA clones that encode an incorrect C-terminal region (T43k.l) and a seco ...
... purified phage from 24 plaques and analysed DNA from these phage by Southern blots. Phage DNA was digested separately with EcoRl and Stul and Southern blots were probed with 32P-T43k.l cDNA. Based upon the restriction maps of cDNA clones that encode an incorrect C-terminal region (T43k.l) and a seco ...
The enduracidin biosynthetic gene cluster from
... genomic DNA. A partial genome scan was performed by endsequencing the inserts of 389 randomly selected clones using the universal T7 promoter primer. Eleven clones were identified as harbouring a portion of the enduracidin or other NRPS gene cluster. Both ends of these inserts were sequenced. The se ...
... genomic DNA. A partial genome scan was performed by endsequencing the inserts of 389 randomly selected clones using the universal T7 promoter primer. Eleven clones were identified as harbouring a portion of the enduracidin or other NRPS gene cluster. Both ends of these inserts were sequenced. The se ...
TAR-RNA binding by HIV-1 Tat protein is
... Nolte’s 38mer L-RNA, originally developed as a ligand for L-arginine, is sequence-wise unrelated to TAR and binds to a short peptide (12 residues) corresponding to the basic region of HIV-1 Tat protein with a Kd of 26 µM. In contrast, our competition binding experiments demonstrate that the affinity ...
... Nolte’s 38mer L-RNA, originally developed as a ligand for L-arginine, is sequence-wise unrelated to TAR and binds to a short peptide (12 residues) corresponding to the basic region of HIV-1 Tat protein with a Kd of 26 µM. In contrast, our competition binding experiments demonstrate that the affinity ...
Cox, G. Nutritional strategies to maximise recovery following
... In normal healthy people, the daily replacement of fluid losses and maintenance of fluid balance are well regulated by thirst and urine losses. However, under conditions of stress (for example, exercise, environmental heat and cold, altitude) thirst may not be a sufficient stimulus for maintaining o ...
... In normal healthy people, the daily replacement of fluid losses and maintenance of fluid balance are well regulated by thirst and urine losses. However, under conditions of stress (for example, exercise, environmental heat and cold, altitude) thirst may not be a sufficient stimulus for maintaining o ...
Biochimica et Biophysica Acta
... Bacteria containing a reporter plasmid and a mutagenized protein expression library were grown on LB agar plates containing ampicillin, chloramphenicol, IPTG, and X-gal. Expression of the repressor plasmid is regulated by an IPTG-dependent promoter, and the amount of IPTG was adjusted to allow maxim ...
... Bacteria containing a reporter plasmid and a mutagenized protein expression library were grown on LB agar plates containing ampicillin, chloramphenicol, IPTG, and X-gal. Expression of the repressor plasmid is regulated by an IPTG-dependent promoter, and the amount of IPTG was adjusted to allow maxim ...
Protein Synthesis and Words - Hewlett
... mRNA from codon to codon, the appropriate amino acids are brought into place and linked together according to the sequence of codons. Thus, the code in the mRNA is translated into a special sequence of amino acids. The order of the amino acids in the protein, therefore, is specified by the mRNA, whi ...
... mRNA from codon to codon, the appropriate amino acids are brought into place and linked together according to the sequence of codons. Thus, the code in the mRNA is translated into a special sequence of amino acids. The order of the amino acids in the protein, therefore, is specified by the mRNA, whi ...
Designed sequences improve distant homology detection
... e-value better than 0.01 in most tested cases where the accepted substitution steps and/or the number of designed sequences added to the native alignment are varied (see supplementary material). Using a multiple alignment of major cold shock proteins directly from PSI-BLAST output for design, we als ...
... e-value better than 0.01 in most tested cases where the accepted substitution steps and/or the number of designed sequences added to the native alignment are varied (see supplementary material). Using a multiple alignment of major cold shock proteins directly from PSI-BLAST output for design, we als ...
Gene Section TMPRSS2 (transmembrane protease, serine 2) Atlas of Genetics and Cytogenetics
... TMPRSS2 is a 492 amino acid single-pass type II membrane protein. It contains a Serine protease domain (aa 255-492) of the S1 family, followed by a Scavenger receptor cysteine-rich domain (SRDR, aa 149-242) of group A; an LDL receptor class A (LDLRA, aa 113-148) domain forms a binding site for calci ...
... TMPRSS2 is a 492 amino acid single-pass type II membrane protein. It contains a Serine protease domain (aa 255-492) of the S1 family, followed by a Scavenger receptor cysteine-rich domain (SRDR, aa 149-242) of group A; an LDL receptor class A (LDLRA, aa 113-148) domain forms a binding site for calci ...
1. Introduction - diss.fu
... chromosomes. Genetic crosses revealed that the mobilization of such element is dependent on a protein product of another element that she named "Activator", or Ac, which itself was able to move by using its own protein product. This transposable element system is called Ac/Ds, and is considered to b ...
... chromosomes. Genetic crosses revealed that the mobilization of such element is dependent on a protein product of another element that she named "Activator", or Ac, which itself was able to move by using its own protein product. This transposable element system is called Ac/Ds, and is considered to b ...
chapter 24
... fatty acids get processed once they’re broken apart Activated by the hormones glucagon or epinephrine ...
... fatty acids get processed once they’re broken apart Activated by the hormones glucagon or epinephrine ...
SecStAnT: secondary structure analysis tool for data selection
... sub-s scale with ordinary computational resources. However, most biologically interesting phenomena occur on wider time and space scales, needing large parallelism. This problem is not likely to be simply resolved by the increase of the processors power and of parallelism, becoming increasingly har ...
... sub-s scale with ordinary computational resources. However, most biologically interesting phenomena occur on wider time and space scales, needing large parallelism. This problem is not likely to be simply resolved by the increase of the processors power and of parallelism, becoming increasingly har ...
Proteins
... • 8. Regulation: Certain proteins not only control the expression of genes, but also control when gene expression takes place. • Proteins are divided into two types: • Fibrous proteins • Globular proteins ...
... • 8. Regulation: Certain proteins not only control the expression of genes, but also control when gene expression takes place. • Proteins are divided into two types: • Fibrous proteins • Globular proteins ...
Chapter 1 Non-Canonical Amino Acids in Protein Engineering
... auxotrophic for both isoleucine and valine, were evaluated as expression hosts for the generation of proteins containing Tfv. The wild-type aaRS activity in these cells was insufficient for production of proteins containing Tfv, but incorporation of one of the diastereomers of Tfv (2S,3R-Tfv) into t ...
... auxotrophic for both isoleucine and valine, were evaluated as expression hosts for the generation of proteins containing Tfv. The wild-type aaRS activity in these cells was insufficient for production of proteins containing Tfv, but incorporation of one of the diastereomers of Tfv (2S,3R-Tfv) into t ...
Info
... tyrosine might also be classified as an acidic amino acid. The phenolic hydroxyl group has a pKa of about 10, which means that the side chain could act as an acid under certain conditions. That's why the "classic" categories can be misleading. Often a given side chain may have several different char ...
... tyrosine might also be classified as an acidic amino acid. The phenolic hydroxyl group has a pKa of about 10, which means that the side chain could act as an acid under certain conditions. That's why the "classic" categories can be misleading. Often a given side chain may have several different char ...
DNAandproteinsynthesis
... Your textbook lacks information on certain sections – use these PP slides as your notes. ...
... Your textbook lacks information on certain sections – use these PP slides as your notes. ...
Glycosylation of Proteins - Structure, Function and Analysis
... Glycosylation is one of the most widely observed, and structurally diverse, forms of post translational modification (PTM) of proteins. Animal, plant, fungal and bacterial cell systems all have the ability to glycosylate proteins and, whilst similarities do exist between these groups, there are also ...
... Glycosylation is one of the most widely observed, and structurally diverse, forms of post translational modification (PTM) of proteins. Animal, plant, fungal and bacterial cell systems all have the ability to glycosylate proteins and, whilst similarities do exist between these groups, there are also ...
Phosphofructokinase (PFK) Exercise
... muscle cramps. Patients with this genetic disorder are advised not to exercise vigorously. Explain why reduced physical activity can help these patients. ...
... muscle cramps. Patients with this genetic disorder are advised not to exercise vigorously. Explain why reduced physical activity can help these patients. ...
Protein Li SDS PAGE
... Staining of samples to become visible Pure proteins and nucleic acids are colourless. To become visible, different methods are used. Dyes that bind aspecifically to every protein is applied frequently. Such dyes are Coomassie Brilliant Blue, Acidic Fast Green, Amido Black, silver bromide. Antibody c ...
... Staining of samples to become visible Pure proteins and nucleic acids are colourless. To become visible, different methods are used. Dyes that bind aspecifically to every protein is applied frequently. Such dyes are Coomassie Brilliant Blue, Acidic Fast Green, Amido Black, silver bromide. Antibody c ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.