• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Answers - Study of Life
Answers - Study of Life

... A. The percent transmission of the first sample will be two times greater than the second sample B. The absorbance, A , of the second sample will be twice the absorbance of the first sample C. The increase in concentration will shift the wavelength of maximum absorbance to a longer wavelength D. The ...
Sec"on 8 - Small World Initiative
Sec"on 8 - Small World Initiative

... •  The  large  and  small  subunit  associate  only  in  the  presence  of  mRNA   •  The  mRNA  passes  through  a  “tunnel”  created  by  the  mature  ribosome   •  This  tunnel  contains  the  ac$ve  A,  P,  and  E  sites  where ...
ah-bio-unit-1-revision-questions
ah-bio-unit-1-revision-questions

... 48. What is meant by the N-terminus and the C-terminus? 49. What are the 2 main types of secondary structure? 50. How are these different arrangements generated? 51. Describe the structure and arrangement of the -helix. 52. Describe the -sheet configuration. 53. -sheets can be either parallel or ...
Cellular Metabolism Pathways
Cellular Metabolism Pathways

... Cancer cells frequently use glutamine as a secondary fuel source, which enters the mitochondria and can be used to replenish Krebs Cycle intermediates or can be used to produce more pyruvate through the action of malic enzyme. Highly proliferative cells need to produce excess lipid, nucleotide, and ...
Preface 1 PDF
Preface 1 PDF

... of yeast as a source of vitamins, good for skin and digestion. Their advertising later claimed a much broader range of health benefits. Nutritional yeast has a nutty, cheesy flavor that makes it popular as an ingredient in cheese substitutes. It is often used by vegetarians in the place of Parmesan c ...
- Wiley Online Library
- Wiley Online Library

... false positive found for this significance level. On the other hand, only a very small number of proteins can be found within the significance levels between 1 and 10, with about a 50050 chance of the prediction in this significance level being wrong. It is interesting to note that the number of cor ...
Lecture 1 - "Hudel" Luecke
Lecture 1 - "Hudel" Luecke

... http://bass.bio.uci.edu/~hudel/bs99a/lecture20/lecture1_1.html (1 of 4)5/25/2007 9:28:46 AM ...
Lecture 3: Introduction to Proteins
Lecture 3: Introduction to Proteins

... Be very familiar with the approximate (“typical”) pK a values of the 7 ionizable R groups (side chains) and also the α-amino and α-carboxyl groups in peptides and proteins; note that numerical values of these "generic " pK a values for the ionizable functional groups in peptides and proteins will be ...
Visualizing the triplet code
Visualizing the triplet code

... Conclusions: Identify which codons specify which amino acids Prove that the code is degenerate ...
Although the administration of testosterone clearly causes marked
Although the administration of testosterone clearly causes marked

... pretreatment from 12 hours to 3 days is illustrated in Table II. In this experiment protein specific activity from both valine-C14 and tyrosine-C04 was doubled within 12 hours and reached a maximum of a five- to sixfold increase within 1 or 2 days after commencing testosterone therapy. It is of part ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle

... bio-accumulated levels of DEHP in clam Venerupis philippinarum. After exposure to 0.4 mg L-1 and 4mg L-1 DEHP, the bio-accumulated DEHP in the clam foot was significantly increased in the first 24 h, and then sharply decreased from 0.203 ± 0.022 μg g-1 to 0.104 ± 0.011 μg g-1 , and from 1.689 ± 0.01 ...
13422_lecture-15-16-week-8-reading-comprehension
13422_lecture-15-16-week-8-reading-comprehension

...  Pea Protein is a complete protein with all essential amino acids and is particularly high in branched chain amino acids as well as arginine, lysine and phenyalaline. It has a very well balanced essential amino acid profile that fits the requirements set by the world health organization for adults. ...
Importance of main-chain hydrophobic free energy to the stability of
Importance of main-chain hydrophobic free energy to the stability of

... spectrum, containing proteins from moderately thermophilic organisms and their mesophilic homologues. Here we select one pair from each family. The three-dimensional structures of all these proteins have been taken from Protein Data Bank [28]. For a given protein, the PDB files contain coordinates f ...
Biology Slide 1 of 39 End Show
Biology Slide 1 of 39 End Show

... (template) ...
ch.6
ch.6

... mass of small peptides (molecular weight). • X-ray Crystallography – determines 3D shape of molecules mathematically • NMR Spectroscopy – magnetic signal indicates distances between atoms ...
ppt - GEP Community Server
ppt - GEP Community Server

... click go to navigate to this region Click on the feature and select “Predicted Protein” to retrieve the predicted protein sequence Select and copy the sequence ...
Protein kinase inhibition: natural and synthetic variations on a theme
Protein kinase inhibition: natural and synthetic variations on a theme

... to be very successful, ...
Protein for Athletes
Protein for Athletes

... Skeletal muscle makes up 40% to 45% of body weight and is the largest storage site for amino acids.1 However, muscle is more than just protein; it also contains water, fat, glycogen, and some minerals. One pound of muscle contains 70 to 105 g of protein, and to build a pound of muscle, it is estimat ...
Finding Patterns in Protein Sequence and Structure
Finding Patterns in Protein Sequence and Structure

... • Many DNA regions code for proteins, and are called genes (1 gene codes for 1 protein in principle) • Human DNA contains ~30,000 expressed genes • Deoxyribonucleic acid (DNA) comprises 4 different types of nucleotides: adenine (A), thiamine (T), cytosine (C) and guanine (G). These nucleotides are s ...
p53
p53

... lower number of genes at any given time. – Differences between cell types are NOT due to different genes but to different gene expression by cells with the same genome. ...
EXAM 1 learning objectives
EXAM 1 learning objectives

... Describe characteristics of amino acids (polar, nonpolar, charged, UV absorption, disulfide bonds) Describe roles of common and uncommon amino acids Understand chirality of amino acids Draw amino acids as acids and/or bases On a titration curve be able to draw all the ionization states of an amino a ...
transcription
transcription

... Stages of Protein Synthesis • The first stage is transcription, in which the information encoded in DNA is copied onto a length of messenger RNA (mRNA). • In eukaryotes, mRNA moves from the cell nucleus to a structure in the cytoplasm called a ribosome. ...
Metabolism - Science Prof Online
Metabolism - Science Prof Online

... PowerPoints, video tutorials, sample assignments and course syllabi. New materials are continually being developed, so check back frequently, or follow us on Facebook (Science Prof Online) or Twitter (ScienceProfSPO) for updates. • Many SPO PowerPoints are available in a variety of formats, such as ...
Core I Objectives - Three-Dimensional Orthopaedic Animations
Core I Objectives - Three-Dimensional Orthopaedic Animations

... Buffer = A buffer is a mixture of a weak acid and its conjugate base. Such a mixture tends to resist changes in pH when either acids or bases are added. The buffering tendency is greatest at pH values near the pKa of the weak acid (pKa = logKa). 2. Explain the difference between strong and weak acid ...
Macromolecules
Macromolecules

...  Specific health conditions, such as pregnancy, breast-feeding, illness, or drug use, make unusual demands on the body and increase its need for nutrients.  Dietary guidelines, which take many of these factors into account, provide general guidance in meeting daily nutritional needs. ...
< 1 ... 148 149 150 151 152 153 154 155 156 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report