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PPT
PPT

... • Amino acids exist as zwitterions, a dipolar ion that results from an internal acid-base reaction. • Note that the net charge of the zwitterion is zero. ...
Organic Compounds: Carbohydrates
Organic Compounds: Carbohydrates

... play critical roles in many biological processes, they do things rather than just form structures, are also called “functional proteins”, not stable  Some are antibodies (provide immunity), hormones (regulate growth & development), enzymes (catalysts for chemical reactions)  Hydrogen bonds are cri ...
Information Extraction from Biomedical Text
Information Extraction from Biomedical Text

... morphological and lexical analysis is used to identify “core terms” (e.g. Src, SH3, p54, SAP) and “feature terms” (e.g. receptor, protein) The focal adhesion kinase (FAK) is... ...
Susan - Stanford University
Susan - Stanford University

... ClusPro is the first integrated automated server that incorporates both docking and discrimination steps for structural predictions of protein-protein complexes Using ClusPro, one can generate many relative orientation/conformations of the 2 proteins  filter using desolvation + electrostatics poten ...
Biomolecules PPT
Biomolecules PPT

... Respiration – energy is released when glucose is broken down to form carbon dioxide and water – catabolism Photosynthesis – glucose molecules are made from carbon dioxide and water using the sun’s energy – anabolism ...
Chapter 3 USU - BEHS Science
Chapter 3 USU - BEHS Science

1.3.6 Structural Role of Biomolecules
1.3.6 Structural Role of Biomolecules

Balance Between Protein Synthesis and Degradation
Balance Between Protein Synthesis and Degradation

... Degradation of a protein begins when it is targeted for destruction by a ubiquitin molecule. Which proteins become ubiquinated depends largely by its amino–terminal residue [10]. This underlying cause of regulation has been highly conserved through millions of years of evolution and across many diff ...
HSPIR: a manually annotated heat shock protein information resource
HSPIR: a manually annotated heat shock protein information resource

... sets were then filtered to include sequences that belong to protein existence level 1 or 2 (evidence at protein level or evidence at the transcript level, respectively). Sequences with domains that are partial in length or missing any functional motifs were discarded. Using these initial data sets a ...
Catalogue Number CTK-551 Synonyms Intelectin-1, HL1, LFR, HL
Catalogue Number CTK-551 Synonyms Intelectin-1, HL1, LFR, HL

... been detected in human blood using western blot analysis, and seems to increase insulin-stimulated glucose uptake in 3T3-L1 adipocytes in mice. Omentin seems to increase Akt phosphorylation irrespective of insulin presence. Its role in glucose metabolism and obesity remains to be described; an insul ...
Slide 1
Slide 1

... that sickle cell haemoglobin varies from wild type by the substitution of one amino acid ...
grasshopper PowerPoint Presentation
grasshopper PowerPoint Presentation

... extraction was done by a cold extraction technique using 2:1 chloroform/methanol as solvent ...
DNA Sequencing and Gene Analysis
DNA Sequencing and Gene Analysis

... of the same base in a row) are notoriously tricky to sequence accurately. Using the opposite strand often helps resolve these regions. Also using a different ...
Protein-Misfolding Diseases
Protein-Misfolding Diseases

... • To help proteins in their folding process • To unfold misfolded proteins before their degradation by the proteasome unit • To protect proteins from interfering interactions during ...
Density Gradient Fractionation System
Density Gradient Fractionation System

... Density Gradient Fractionation System ...
Macromolecules Quiz
Macromolecules Quiz

... Matching--Select the macromolecule that best matches the statement. Letters may be used once, more than once or not at all. a. Proteins b. Carbohydrates c. Lipids d. Nucleic Acids 1. These macromolecules possess large nonpolar regions making them insoluble in water. 2. This macromolecule is compose ...
Example Problem Set for CHEM106 Section 002 Test 2
Example Problem Set for CHEM106 Section 002 Test 2

... 10) Describe, give the advantage(s) of and give an example of an enzyme that uses the following enzymatic control mechanisms: a) Phosphorylation b) Feedback Inhibition c) Production as a zymogen 11) What are the 2 primary secondary structural elements in proteins? List the distinguishing features of ...
5lb (2270 g) - BioTech USA
5lb (2270 g) - BioTech USA

... THE PROTEIN BASE WAS DEVELOPED ACCORDING TO THE HIGHEST QUALITY REQUIREMENTS DERIVED FROM THE ONLY CONTINUOUSLY CONTROLLED FARM IN THE UNITED STATES. ONE OF THE HIGHEST QUALITY MILK PROTEINS IS DERIVED FROM THE MILK THAT ARRIVES DAILY FOR USE IN PHARMACEUTICALS. THE WHEY PROTEIN COMPLEX IS DERIVED F ...
Lecture 1 - Temple University
Lecture 1 - Temple University

... because of steric collisions between atoms within each amino acid, most pairs of f and y angles do not occur. In this so-called Ramachandran plot, each dot represents an observed pair of angles in a protein. (B, from J. Richardson, Adv. Prot. Chem. 34:174 175, 1981. © Academic Press ...
Proteins Introduction Aspects of a protein`s structure Primary
Proteins Introduction Aspects of a protein`s structure Primary

... • Globular proteins are by far the most abundant class of proteins. Many of the most heavily studied proteins are members of this class of proteins. • They are a highly diverse group of proteins that are soluble and form compact spheroidal molecules in water. • All have tertiary structure and some h ...
Laboratory Technician-Bioanalytical Methods PLASMA PROTEIN
Laboratory Technician-Bioanalytical Methods PLASMA PROTEIN

... areas of anaemia, liver disease and organ preservation; and Therapure Biologics dedicated to the development of plasma protein therapeutics through an innovative proprietary manufacturing technology. We are growing! In fact, in 2014, we were recognized by Profit 500 as one of Canada’s Fastest Growin ...
protein - Portal UniMAP
protein - Portal UniMAP

... Proteins consist of two or more polypeptide chains aggregated into one functional macromolecules Many proteins, esp those with high molecular weight are composed of several polypeptide chains. In proteins that consist of more than 1 polypeptide chain, each polypeptide is called subunit Polypeptide s ...
Essential Amino Acids
Essential Amino Acids

... molecules, and any excess proteins you eat are broken down into their amino acids and used for energy or converted and stored as fat. Protein breakdown produces the waste urea, which is filtered from the blood by the kidneys. Urea is what gives your urine its yellow color. Why protein is needed Diet ...
Supplementary Material: Settings and other parameters of the
Supplementary Material: Settings and other parameters of the

AP BIOLOGY STUDY GUIDE: CH 17, FROM GENE TO PROTEIN
AP BIOLOGY STUDY GUIDE: CH 17, FROM GENE TO PROTEIN

... AP BIOLOGY STUDY GUIDE: CH 17, FROM GENE TO PROTEIN The Gene—Protein Connection ...
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Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
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