Membrane Proteins Integral membrane proteins often contain
... Integral membrane proteins often contain helical segments of appropriate length to span the lipid bilayer. In a protein that has a single segment that spans the membrane, the helix usually only contains hydrophobic residues and is called a single-span membrane protein. In transmembrane proteins with ...
... Integral membrane proteins often contain helical segments of appropriate length to span the lipid bilayer. In a protein that has a single segment that spans the membrane, the helix usually only contains hydrophobic residues and is called a single-span membrane protein. In transmembrane proteins with ...
36-1577: Monoclonal Antibody to UACA / Nucling (Nuclear
... was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle different ...
... was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle different ...
charge-to-mass ratio. The electrophoretic mobility is defined as the
... similarity between the above equation and that used for gel filtration. For example, if hemoglobin were run as a standard, it would result in a band on the gel at a mobility corresponding to Mr = 16 kDa, i.e. its monomer molecular weight and myoglobin (Mr = 17 kDa) would be nearby because it is a si ...
... similarity between the above equation and that used for gel filtration. For example, if hemoglobin were run as a standard, it would result in a band on the gel at a mobility corresponding to Mr = 16 kDa, i.e. its monomer molecular weight and myoglobin (Mr = 17 kDa) would be nearby because it is a si ...
Human Proteome advertising miniposter (PDF)
... Antibodies, also known as immunoglobulins, are Y-shaped proteins, which are used by the immune system to identify and destroy foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, the antigen. The unique properties of antibodies are used in a wide ...
... Antibodies, also known as immunoglobulins, are Y-shaped proteins, which are used by the immune system to identify and destroy foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, the antigen. The unique properties of antibodies are used in a wide ...
Search for the potential antigens present in the outer membrane of
... Gram-negative coccobacillus pathogen which is Pasteurella multocida result in fowl cholera and hemorrhagic septicaemia. Current vaccines against fowl cholera including the live naturally attenuated and the killed whole cells, provide only limited protection. In this study, we tried to find the possi ...
... Gram-negative coccobacillus pathogen which is Pasteurella multocida result in fowl cholera and hemorrhagic septicaemia. Current vaccines against fowl cholera including the live naturally attenuated and the killed whole cells, provide only limited protection. In this study, we tried to find the possi ...
SouthernHybridization - University of Hawaii
... a) All bands in all lanes are alternative forms of PDI-2. b) Anti-PDI peptide antibody from rabbit reacts with similar epitopes on unrelated proteins. c) 2° antibody from donkey reacts to similar epitopes on unrelated proteins. ...
... a) All bands in all lanes are alternative forms of PDI-2. b) Anti-PDI peptide antibody from rabbit reacts with similar epitopes on unrelated proteins. c) 2° antibody from donkey reacts to similar epitopes on unrelated proteins. ...
general western blot troubleshooting tips
... Filter the secondary with a 0.2 µm filter to remove any aggregates. ...
... Filter the secondary with a 0.2 µm filter to remove any aggregates. ...
Anti-MARCH6 antibody ab56594 Product datasheet 1 References 1 Image
... Recombinant fragment: DTAEEDICRV CRSEGTPEKP LYHPCVCTGS IKFIHQECLV QWLKHSRKEY CELCKHRFAF TPIYSPDMPS RLPIQDIFAG LVTSIGTAIR , corresponding to amino acids 2-92 of Human MARCH6 ...
... Recombinant fragment: DTAEEDICRV CRSEGTPEKP LYHPCVCTGS IKFIHQECLV QWLKHSRKEY CELCKHRFAF TPIYSPDMPS RLPIQDIFAG LVTSIGTAIR , corresponding to amino acids 2-92 of Human MARCH6 ...
Steps in a Western blot
... substrate molecule that will be converted by the enzyme to a colored reaction product that will be visible on the membrane (see the figure below with blue bands). ...
... substrate molecule that will be converted by the enzyme to a colored reaction product that will be visible on the membrane (see the figure below with blue bands). ...
Supplemental Materials and Methods
... synthetic DNA (Life Technologies) with Gateway attB1 and attB2 sites flanking the insert region. His6MBP (maltose binding protein) tags were used to enhance solubility and permit affinity purification of the final dimers. The Entry clones were subcloned into pDest-636 Gateway LR recombination (Life ...
... synthetic DNA (Life Technologies) with Gateway attB1 and attB2 sites flanking the insert region. His6MBP (maltose binding protein) tags were used to enhance solubility and permit affinity purification of the final dimers. The Entry clones were subcloned into pDest-636 Gateway LR recombination (Life ...
Chapter 3
... Figure 03.13A: The light and heavy chains interact to form binding sites at the tips of the short arms of the antibody. ...
... Figure 03.13A: The light and heavy chains interact to form binding sites at the tips of the short arms of the antibody. ...
Extended information on Western blot quantification To Gassen et al
... time as the protein of interest (different size, ECL) and used for normalization. Only one Actin example is provided in the figures. Some figures show blots where sequential detection has been applied. Figure S2 provides an example of the different procedures. In panel A, Atg12 and pAktS473 are in s ...
... time as the protein of interest (different size, ECL) and used for normalization. Only one Actin example is provided in the figures. Some figures show blots where sequential detection has been applied. Figure S2 provides an example of the different procedures. In panel A, Atg12 and pAktS473 are in s ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.