BIO315
... functions in that they are both involved in transport within the cell, so they are similar. However, their functions are very distinct, & so are their sequences. ...
... functions in that they are both involved in transport within the cell, so they are similar. However, their functions are very distinct, & so are their sequences. ...
The phosphatidylinositol transfer protein RdgBβ binds 14-3
... (monoclonal antibody) anti-HA (haemagglutinin) [HA-Probe (F7)] (sc-7392). The mAb anti-FLAG (DDK) was obtained from OriGene Technologies (TA50011) and the pAb anti-ubiquitin (U5379) was from Sigma–Aldrich. An anti-PITPNC1 pAb was obtained from Orbigen (PAB-02250) and the anti-ATRAP mAb (ab57555) was ...
... (monoclonal antibody) anti-HA (haemagglutinin) [HA-Probe (F7)] (sc-7392). The mAb anti-FLAG (DDK) was obtained from OriGene Technologies (TA50011) and the pAb anti-ubiquitin (U5379) was from Sigma–Aldrich. An anti-PITPNC1 pAb was obtained from Orbigen (PAB-02250) and the anti-ATRAP mAb (ab57555) was ...
Adenovirus-associated Virus Structural Protein Sequence Homology
... serum albumin as the standard protein. Radioiodination of virion protein. Purified virions were disrupted by treatment in I.O~o SDS at loo °C for 2 min. Samples containing 3o to 5o/zg of virus protein were suspended in 5o/zl of o'o5 M-phosphate buffer, pH 7'4 (buffer I), and the proteins iodinated b ...
... serum albumin as the standard protein. Radioiodination of virion protein. Purified virions were disrupted by treatment in I.O~o SDS at loo °C for 2 min. Samples containing 3o to 5o/zg of virus protein were suspended in 5o/zl of o'o5 M-phosphate buffer, pH 7'4 (buffer I), and the proteins iodinated b ...
Hints on Column Chromatography
... • High levels of antioxidants - complex mixtures of phenolic comounds • By weight, has higher concentration of antioxidants than red wine or Green tea • 20x higher concentration of antioxidants than tomatoes ...
... • High levels of antioxidants - complex mixtures of phenolic comounds • By weight, has higher concentration of antioxidants than red wine or Green tea • 20x higher concentration of antioxidants than tomatoes ...
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... and temperature, each protein folds spontaneously into one three-dimensional conformation, called the native conformation. 3.1.2 This conformation is usually thermodynamically the most stable (having the lowest Gibb’s free energy), and predominates among the innumerable theoretically possible ones. ...
... and temperature, each protein folds spontaneously into one three-dimensional conformation, called the native conformation. 3.1.2 This conformation is usually thermodynamically the most stable (having the lowest Gibb’s free energy), and predominates among the innumerable theoretically possible ones. ...
Biology Standards Based Benchmark Assessment
... of the cell membrane has two main parts: the head and the tail. These parts are either hydrophobic (repelled by water) or hydrophilic (attracted to water). Which statement best describe the phospholipid head and tail? a. The tail is hydrophilic and the head is hydrophobic. b. The head is hydrophilic ...
... of the cell membrane has two main parts: the head and the tail. These parts are either hydrophobic (repelled by water) or hydrophilic (attracted to water). Which statement best describe the phospholipid head and tail? a. The tail is hydrophilic and the head is hydrophobic. b. The head is hydrophilic ...
![Mouse (monoclonal) anti-β-Catenin [pY86]](http://s1.studyres.com/store/data/013277785_1-9ecc4a36fe4e6b90692b9558e5656402-300x300.png)
Mouse (monoclonal) anti-β-Catenin [pY86]
... of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. βcate ...
... of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. βcate ...
Production of final product
... - Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) : most commonly used analytical technique - high resolution separation of proteins based on their molecular mass - band containing as little as 100 ng protein can be visualized by staining the gel with dyes like Coomassie blue ...
... - Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) : most commonly used analytical technique - high resolution separation of proteins based on their molecular mass - band containing as little as 100 ng protein can be visualized by staining the gel with dyes like Coomassie blue ...
Document
... spaced in elastin). Elastin fibers are much more elastic than collagen because they do not contain hydroxylysine or hydroxyproline. The elasticity of the fibers allows tissues to resume their original shape after significant stretching. Hemoglobin (Hb): Hemoglobin is a component of red blood cells (RBC ...
... spaced in elastin). Elastin fibers are much more elastic than collagen because they do not contain hydroxylysine or hydroxyproline. The elasticity of the fibers allows tissues to resume their original shape after significant stretching. Hemoglobin (Hb): Hemoglobin is a component of red blood cells (RBC ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... b. Write an account on phenylketonuria and Gaucher’s disease. 24. a. Illustrate the principle and instrumentation of UV spectroscopy. OR b. Employ a non-invasive technique to image fetal movements. . 25. a. Explain the torsion angles using Ramachandran plot. OR b. Briefly explain Bragg’s law and bio ...
... b. Write an account on phenylketonuria and Gaucher’s disease. 24. a. Illustrate the principle and instrumentation of UV spectroscopy. OR b. Employ a non-invasive technique to image fetal movements. . 25. a. Explain the torsion angles using Ramachandran plot. OR b. Briefly explain Bragg’s law and bio ...
word - My eCoach
... makes substrate collisions less frequent. c. The optimal temperature of this enzyme is 25oC. d. Enzyme synthesis does not take place at high temperatures. ...
... makes substrate collisions less frequent. c. The optimal temperature of this enzyme is 25oC. d. Enzyme synthesis does not take place at high temperatures. ...
plasma membrane
... membrane also consists of carbohydrates, basically glycoproteins and glycolipids. These molecules are exclusively arranged in the outer side of the cell membrane, wherein the carbohydrate portions are exposed to the external surface of the cell. ...
... membrane also consists of carbohydrates, basically glycoproteins and glycolipids. These molecules are exclusively arranged in the outer side of the cell membrane, wherein the carbohydrate portions are exposed to the external surface of the cell. ...
AMINO ACIDS AND PROTEINS THEORY Proteins are one of the
... protein. Denaturation is an irreversible process. Some common denaturing agents are alcohols, metal ions, strong acids and bases and heat. ...
... protein. Denaturation is an irreversible process. Some common denaturing agents are alcohols, metal ions, strong acids and bases and heat. ...
(key)
... b. What is the main functionality/functional group of the compounds you choose above that gives rise to their ability to harvest light. C6n.J"J~d Aoublt B;;.J; ...
... b. What is the main functionality/functional group of the compounds you choose above that gives rise to their ability to harvest light. C6n.J"J~d Aoublt B;;.J; ...
Gene Section HSPA5 (heat shock 70kDa protein 5 (glucose regulated protein, 78kDa)) -
... domain. Its functional cycle involves an ATP-form with low affinity for substrate (poly)peptides and an ADP-form with high substrate affinity and is regulated by Hsp40-type co-chaperones and nucleotide exchange factors. Molecular chaperones of the Hsp70 type family reversibly bind to substrate polyp ...
... domain. Its functional cycle involves an ATP-form with low affinity for substrate (poly)peptides and an ADP-form with high substrate affinity and is regulated by Hsp40-type co-chaperones and nucleotide exchange factors. Molecular chaperones of the Hsp70 type family reversibly bind to substrate polyp ...
REVIEWS How membrane proteins travel across the mitochondrial
... or Tim8p–Tim13p is bound directly to the precursor. Equally plausible is a model in which the 70 kDa complexes form a link between the TOM and the TIM complexes. In this model, the precursor is not released into the intermembrane space but binds to the small Tim proteins as it emerges from the TOM c ...
... or Tim8p–Tim13p is bound directly to the precursor. Equally plausible is a model in which the 70 kDa complexes form a link between the TOM and the TIM complexes. In this model, the precursor is not released into the intermembrane space but binds to the small Tim proteins as it emerges from the TOM c ...
Copper(II) sulfate pentahydrate ACS Reagent Product Number
... 6. Dihazi, H., et al., One-step purification of recombinant yeast 6-phosphofructo-2-kinase after the identification of contaminants by MALDI-TOF MS. Protein Expr. Purif., 21(1), 201-209 (2001). 7. Fernandes, S., et al., Affinity extraction of dye- and metal ion-binding proteins in polyvinylpyrrolido ...
... 6. Dihazi, H., et al., One-step purification of recombinant yeast 6-phosphofructo-2-kinase after the identification of contaminants by MALDI-TOF MS. Protein Expr. Purif., 21(1), 201-209 (2001). 7. Fernandes, S., et al., Affinity extraction of dye- and metal ion-binding proteins in polyvinylpyrrolido ...
use of tobacco plants as bioreactors for the production of human
... mannose 6-phosphate, we tried to understand what kind of mechanism uses this human protein in plants to reach the vacuole. Studies performed on transgenic leaf protoplasts using different drugs which inhibit protein traffic and glycosylation indicate that traffic to the vacuole of the precursor MAN2 ...
... mannose 6-phosphate, we tried to understand what kind of mechanism uses this human protein in plants to reach the vacuole. Studies performed on transgenic leaf protoplasts using different drugs which inhibit protein traffic and glycosylation indicate that traffic to the vacuole of the precursor MAN2 ...
embj201490361-sup-0002
... the accuracy of the intact measurement is typically better than 3 Da for a protein with a mass of 50K Da. B An expanded region of the mass spectrum focusing on the peak for TRIM5αrh. Note also the absence of species corresponding to unacetylated, demethionylated TRIM5α or methionylated TRIM5. ...
... the accuracy of the intact measurement is typically better than 3 Da for a protein with a mass of 50K Da. B An expanded region of the mass spectrum focusing on the peak for TRIM5αrh. Note also the absence of species corresponding to unacetylated, demethionylated TRIM5α or methionylated TRIM5. ...
Substrate
... labeled by random primed DNA labeling using dioxigenin-dUTP as probe. Conditions of hydridization were: pre-hybridization 1 hr at 65ºC and hybridization o/n at 65ºC, in standard buffer: 5x SSC, 0.1% N-lauroylsarcosine, 0.02% SDS, 1% blocking reagent (#1096176 from Roche Applied Science) dissolved in ...
... labeled by random primed DNA labeling using dioxigenin-dUTP as probe. Conditions of hydridization were: pre-hybridization 1 hr at 65ºC and hybridization o/n at 65ºC, in standard buffer: 5x SSC, 0.1% N-lauroylsarcosine, 0.02% SDS, 1% blocking reagent (#1096176 from Roche Applied Science) dissolved in ...
20. Biochemistry of Muscles and Connective Tissue
... •Structural unit of miofibril sarcomer •Both ends of thick miosin filaments are free •One end of thin filaments is attached to Z-plate ...
... •Structural unit of miofibril sarcomer •Both ends of thick miosin filaments are free •One end of thin filaments is attached to Z-plate ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.