Signaling mechanistics: Aluminum fluoride for
... 105-fold by GTPase-activating proteins (RasGAPs) that downregulate the accumulation of Ras⋅GTP. Ras binds aluminum fluoride only in the presence of RasGAP, and an efficient GTPase site is only created by the addition of stoichiometric amounts of RasGAP. One particular arginine residue is required fo ...
... 105-fold by GTPase-activating proteins (RasGAPs) that downregulate the accumulation of Ras⋅GTP. Ras binds aluminum fluoride only in the presence of RasGAP, and an efficient GTPase site is only created by the addition of stoichiometric amounts of RasGAP. One particular arginine residue is required fo ...
DNA:chromatin interactions
... These proteins serve to attract co-factors, which then mediate protein: protein interactions across chromatin loops Very long range interactions are common in vertebrates, less so in invertebrate species with lower coding:nocoding ratios ChIP with an antibody that binds to “E” DNA will bring down “P ...
... These proteins serve to attract co-factors, which then mediate protein: protein interactions across chromatin loops Very long range interactions are common in vertebrates, less so in invertebrate species with lower coding:nocoding ratios ChIP with an antibody that binds to “E” DNA will bring down “P ...
5 x buffer (50TB 25 7 - American Journal of Physiology
... factor did bind to a cation resin at pH 5.8 but not at 6.3 and 7.8. The factor did also bind to anion resin at pH 7.8 and at 6.3 with a lower affinity, but not at 5.8 (data not shown). This suggested that the isoelectric point for the protein(s) was between 5.8-6.3. We then performed anion exchange ...
... factor did bind to a cation resin at pH 5.8 but not at 6.3 and 7.8. The factor did also bind to anion resin at pH 7.8 and at 6.3 with a lower affinity, but not at 5.8 (data not shown). This suggested that the isoelectric point for the protein(s) was between 5.8-6.3. We then performed anion exchange ...
Lecture 25
... Antibodies to tumor antigens have advantages over other serum proteins as potential cancer biomarkers as they are stable, highly specific, easily purified from serum, and are readily detected with well-validated secondary reagents. The antibodies directed at self-antigens are referred to as autoanti ...
... Antibodies to tumor antigens have advantages over other serum proteins as potential cancer biomarkers as they are stable, highly specific, easily purified from serum, and are readily detected with well-validated secondary reagents. The antibodies directed at self-antigens are referred to as autoanti ...
Worksheet/Learning Aid Key
... formation of a polycistronic mRNA, which represents an RNA molecule with coding sequences for protein synthesis of separate proteins. 4. Separate ribosomes are assembled on the polycistronic mRNA and initiate translation followed by termination of individual polypeptides or proteins ...
... formation of a polycistronic mRNA, which represents an RNA molecule with coding sequences for protein synthesis of separate proteins. 4. Separate ribosomes are assembled on the polycistronic mRNA and initiate translation followed by termination of individual polypeptides or proteins ...
File
... d. More than one is correct e. None of the above 48. Which of the following metabolic poisons will interfere with Glycolysis? a. Rotenone and Antimycin; electron transport inhibitors b. Carbony cyanide p-rifluoromethoxyle; Mimics 3D structure of glucose and cannot be metabolized by the cell. c. Malo ...
... d. More than one is correct e. None of the above 48. Which of the following metabolic poisons will interfere with Glycolysis? a. Rotenone and Antimycin; electron transport inhibitors b. Carbony cyanide p-rifluoromethoxyle; Mimics 3D structure of glucose and cannot be metabolized by the cell. c. Malo ...
Modelling Argonaute protein interactions as predictors of local
... PICK1. Experimental data from our lab indicate that at least some of these interactions are regulated by the induction of Long-Term Depression (LTD), causing an increase in RISC activity, and hence providing a mechanism for increasing miRNA-mediated translational repression as a part of the expressi ...
... PICK1. Experimental data from our lab indicate that at least some of these interactions are regulated by the induction of Long-Term Depression (LTD), causing an increase in RISC activity, and hence providing a mechanism for increasing miRNA-mediated translational repression as a part of the expressi ...
Chapter 21 - Cengage Learning
... The amino acid cysteine helps proteins maintain their unique tertiary structures. The –SH side chains of two cysteine molecules can react to form a disulfide linkage that holds the protein chain in a fixed tertiary structure. ...
... The amino acid cysteine helps proteins maintain their unique tertiary structures. The –SH side chains of two cysteine molecules can react to form a disulfide linkage that holds the protein chain in a fixed tertiary structure. ...
SCJ4600048 - Sigma
... This procedure is a guideline for labeling IgG antibodies in bicarbonate buffer. Procedures for labeling other proteins can be modified accordingly. The procedure is for labeling 5 mg of an IgG antibody in bicarbonate buffer. The procedure may be scaled up or down for any amount of protein as long a ...
... This procedure is a guideline for labeling IgG antibodies in bicarbonate buffer. Procedures for labeling other proteins can be modified accordingly. The procedure is for labeling 5 mg of an IgG antibody in bicarbonate buffer. The procedure may be scaled up or down for any amount of protein as long a ...
The rational drug design is one of the major challenges in structural
... specificity. The major prerequisite to develop such drugs is to understand the targets that these agents should attack. In recent years a number of promising new anticancer drugs have been developed which target intracellular pathways or extracellular cell molecules. The clinically most effective co ...
... specificity. The major prerequisite to develop such drugs is to understand the targets that these agents should attack. In recent years a number of promising new anticancer drugs have been developed which target intracellular pathways or extracellular cell molecules. The clinically most effective co ...
Active Transport
... A student investigated the effect of pH on the activity of the enzyme amylase. She set up the apparatus shown in the diagram. The tubes were made from Visking tubing. Visking tubing is partially permeable. She added an equal volume of amylase solution and starch to each tube. ...
... A student investigated the effect of pH on the activity of the enzyme amylase. She set up the apparatus shown in the diagram. The tubes were made from Visking tubing. Visking tubing is partially permeable. She added an equal volume of amylase solution and starch to each tube. ...
Plant Enzyme Structure. Explaining Substrate
... considerably (Fig. 1). By the beginning of 2000 about 140 individual plant protein structures were known, of which 37 related to individual plant enzymes. Most 3D structural data have been generated by x-ray crystallography. The first and very often the limiting step in this procedure is the product ...
... considerably (Fig. 1). By the beginning of 2000 about 140 individual plant protein structures were known, of which 37 related to individual plant enzymes. Most 3D structural data have been generated by x-ray crystallography. The first and very often the limiting step in this procedure is the product ...
Validating the Location of Fluorescent Protein
... endogenous localization of one isoform in cells that also express the FPF to another isoform, which acts as an internal control. This will also control for changes to FPF localization that may occur under conditions of immunofluorescence (see below). This strategy was adopted recently to validate th ...
... endogenous localization of one isoform in cells that also express the FPF to another isoform, which acts as an internal control. This will also control for changes to FPF localization that may occur under conditions of immunofluorescence (see below). This strategy was adopted recently to validate th ...
BIOMG 3310: Principles of Biochemistry
... This also causes the loss of one positive and one negative charge, which changes the amino acid behavior because the behavior of the free amino acid is dependent upon the amino acid having two charges. ...
... This also causes the loss of one positive and one negative charge, which changes the amino acid behavior because the behavior of the free amino acid is dependent upon the amino acid having two charges. ...
Lecture 4: bioenergetics and metabolism (mitochondria and
... Small, membrane-enclosed organelles They contain enzymes involved in a variety of metabolic reactions, including several aspects of energy metabolism. They replicate by division. Most human cells contain 500 peroxisomes. ...
... Small, membrane-enclosed organelles They contain enzymes involved in a variety of metabolic reactions, including several aspects of energy metabolism. They replicate by division. Most human cells contain 500 peroxisomes. ...
Bacterial Evolution: Sample Risk Assessment
... Please note: centres should assess risks and produce their own risk assessment Activity Micropipetting practice, restriction enzyme digest and gel loading ...
... Please note: centres should assess risks and produce their own risk assessment Activity Micropipetting practice, restriction enzyme digest and gel loading ...
BIOCHEMISTRY 2.1
... and nucleic acids) and their functions in biological systems—CHO focus on glucose polymers including chitin, starch, cellulose, glycogen; Proteins intro enzymes and give common examples including hemoglobin, antibodies, collagen, muscle fibers, hair, nails, and cell fibers actin and myosin; Nucleic ...
... and nucleic acids) and their functions in biological systems—CHO focus on glucose polymers including chitin, starch, cellulose, glycogen; Proteins intro enzymes and give common examples including hemoglobin, antibodies, collagen, muscle fibers, hair, nails, and cell fibers actin and myosin; Nucleic ...
Level 2 Biology - No Brain Too Small
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
Crenarchaeal CdvA Forms Double-Helical Filaments Containing
... ESCRT-III [22]. The C-terminus of the archaeal CdvB contains a MIT domain interacting motif (MIM2) that interacts with CdvC [10], similar to the eukaryotic Vps4-MIT-CHMP6 interaction [23]. Consistent with eukaryotic ESCRT function, overexpression of a catalytically inactive CdvC (Vps4) mutant in Sul ...
... ESCRT-III [22]. The C-terminus of the archaeal CdvB contains a MIT domain interacting motif (MIM2) that interacts with CdvC [10], similar to the eukaryotic Vps4-MIT-CHMP6 interaction [23]. Consistent with eukaryotic ESCRT function, overexpression of a catalytically inactive CdvC (Vps4) mutant in Sul ...
Chapter 7 Control of Microbial Growth
... • Nucleic acid (DNA) vaccines – West Nile (for horses) – DNA injected into skin cells, protein made – stimulates immune system. ...
... • Nucleic acid (DNA) vaccines – West Nile (for horses) – DNA injected into skin cells, protein made – stimulates immune system. ...
Protein Synthesis - No Brain Too Small
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
Three main topics for this Intro lecture
... Predicting Post-translational Modifications • Post-translational modifications often occur on similar motifs in different proteins • PROSITE is a database containing a list of known motifs, each associated with a function or a post-translational modification • You can search PROSITE by looking for ...
... Predicting Post-translational Modifications • Post-translational modifications often occur on similar motifs in different proteins • PROSITE is a database containing a list of known motifs, each associated with a function or a post-translational modification • You can search PROSITE by looking for ...
Gene Section EIF4EBP1 (Eukaryotic translation initiation factor 4E binding protein 1)
... The members of the 4E-BP family of proteins act by binding to the mRNA cap-binding protein eukaryotic initiation factor 4E (eIF4E), in compe-tition with another initiation factor, eIF4G, that is essential for polypeptide chain initiation. Thus the availability of eIF4E for translation of cap-depende ...
... The members of the 4E-BP family of proteins act by binding to the mRNA cap-binding protein eukaryotic initiation factor 4E (eIF4E), in compe-tition with another initiation factor, eIF4G, that is essential for polypeptide chain initiation. Thus the availability of eIF4E for translation of cap-depende ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.