Gene Section EIF4EBP1 (Eukaryotic translation initiation factor 4E binding protein 1)
... The members of the 4E-BP family of proteins act by binding to the mRNA cap-binding protein eukaryotic initiation factor 4E (eIF4E), in compe-tition with another initiation factor, eIF4G, that is essential for polypeptide chain initiation. Thus the availability of eIF4E for translation of cap-depende ...
... The members of the 4E-BP family of proteins act by binding to the mRNA cap-binding protein eukaryotic initiation factor 4E (eIF4E), in compe-tition with another initiation factor, eIF4G, that is essential for polypeptide chain initiation. Thus the availability of eIF4E for translation of cap-depende ...
Final Exam Study Guide
... b. Describe which way water will move and explain your answer. Water will move from the right side to the left. (b/c higher water potential on the right than on the left. Water moves form high water potential to lower water potential) - DNA polymerase from T. aquaticus (Taq) is used in PCR (polymera ...
... b. Describe which way water will move and explain your answer. Water will move from the right side to the left. (b/c higher water potential on the right than on the left. Water moves form high water potential to lower water potential) - DNA polymerase from T. aquaticus (Taq) is used in PCR (polymera ...
Probs 2 KEY 240 spr06
... 5. What is meant by the "primary structure" of a protein. Basically, the amino acid sequence of the protein. This phrase includes the covalent bonds in a polypeptide, and so it can be meant to include disulfide bonds. 6. What are the differences between parallel and antiparallel Β-sheets? Parallel b ...
... 5. What is meant by the "primary structure" of a protein. Basically, the amino acid sequence of the protein. This phrase includes the covalent bonds in a polypeptide, and so it can be meant to include disulfide bonds. 6. What are the differences between parallel and antiparallel Β-sheets? Parallel b ...
ATP
... Protein Structure • 2 or more amino acids joined by peptide bond –Hence the other name for a protein: polypeptide chain ...
... Protein Structure • 2 or more amino acids joined by peptide bond –Hence the other name for a protein: polypeptide chain ...
The cell membrane surrounds all cells and it is selectively
... The prokaryote cell is simpler, and therefore smaller, than a eukaryote Cell. Lacking a nucleus and most of the other organelles of eukaryotes. Nuclear material of prokaryotic cell consist of a single chromosome which is in direct contact with cytoplasm. A prokaryotic cell has three architectural re ...
... The prokaryote cell is simpler, and therefore smaller, than a eukaryote Cell. Lacking a nucleus and most of the other organelles of eukaryotes. Nuclear material of prokaryotic cell consist of a single chromosome which is in direct contact with cytoplasm. A prokaryotic cell has three architectural re ...
Organic Notes.graffle
... thousands of different proteins found in a single cell. If the 20 different amino acids are put together in various combinations there can be endless numbers of proteins. ...
... thousands of different proteins found in a single cell. If the 20 different amino acids are put together in various combinations there can be endless numbers of proteins. ...
Key concepts_chromatin
... The genomes of all organisms are compacted by interaction with specific proteins. These often play roles in gene regulation. In bacteria, a large circular chromosome is complexed by proteins in a dynamic manner that compacts it and yet makes it accessible for transcription. Eukaryotes utilize a set ...
... The genomes of all organisms are compacted by interaction with specific proteins. These often play roles in gene regulation. In bacteria, a large circular chromosome is complexed by proteins in a dynamic manner that compacts it and yet makes it accessible for transcription. Eukaryotes utilize a set ...
![Anti-Hepatitis C Virus Core Antigen antibody [1F6] (Biotin)](http://s1.studyres.com/store/data/006232959_1-bbbfad1dc36a2ae8eac92eac11846bec-300x300.png)
Anti-Hepatitis C Virus Core Antigen antibody [1F6] (Biotin)
... protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infecte ...
... protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infecte ...
Moonlighting proteins—an update
... protein to switch between functions, including secretion into the extracellular space, interactions with DNA or RNA, a change in temperature, a change in the redox state of the cell, a change in oligomeric state of the protein, interactions with different polypeptide chains in different protein comple ...
... protein to switch between functions, including secretion into the extracellular space, interactions with DNA or RNA, a change in temperature, a change in the redox state of the cell, a change in oligomeric state of the protein, interactions with different polypeptide chains in different protein comple ...
Lecture Notes of Seminario Interdisciplinare di Matematica Vol. 9
... optimization techniques for conformational search is Conformational Space Annealing (CSA) introduced by Lee, et al [Lee, et al, 1997]. CSA has been designed to search a large portion of the potential energy surface. It is an iterative algorithm maintaining in each iteration a population of local min ...
... optimization techniques for conformational search is Conformational Space Annealing (CSA) introduced by Lee, et al [Lee, et al, 1997]. CSA has been designed to search a large portion of the potential energy surface. It is an iterative algorithm maintaining in each iteration a population of local min ...
A History of Computing
... • Keep in mind that BLAST is a heuristic version of Smith-Waterman, and may miss a significant alignment. The following examples, kindly provided by Christopher Dwan of The University of Minnesota Center for Computational Genomics and Bioinformatics, illustrate the consequences of choice of matrix a ...
... • Keep in mind that BLAST is a heuristic version of Smith-Waterman, and may miss a significant alignment. The following examples, kindly provided by Christopher Dwan of The University of Minnesota Center for Computational Genomics and Bioinformatics, illustrate the consequences of choice of matrix a ...
Validating the Location of Fluorescent Protein
... endogenous localization of one isoform in cells that also express the FPF to another isoform, which acts as an internal control. This will also control for changes to FPF localization that may occur under conditions of immunofluorescence (see below). This strategy was adopted recently to validate th ...
... endogenous localization of one isoform in cells that also express the FPF to another isoform, which acts as an internal control. This will also control for changes to FPF localization that may occur under conditions of immunofluorescence (see below). This strategy was adopted recently to validate th ...
HTRF phospho-IKKβ is more sensitive than Western-Blot
... proteins in whole cells. With 12 phosphospecific assays, it covers key targets implicated in diverse signaling pathways, and enables an accurate and sensitive determination of phosphorylation events in whole cells using the same technology. In this study, a panel of HTRF cellular kinase assays was c ...
... proteins in whole cells. With 12 phosphospecific assays, it covers key targets implicated in diverse signaling pathways, and enables an accurate and sensitive determination of phosphorylation events in whole cells using the same technology. In this study, a panel of HTRF cellular kinase assays was c ...
Organic Compounds
... Enzymes are a type of protein that speed up chemical reactions. They are known as "biological catalysts." For example, your stomach would not be able to break down food if it did not have special enzymes to speed up the rate of digestion. Antibodies that protect you against disease are proteins. Mus ...
... Enzymes are a type of protein that speed up chemical reactions. They are known as "biological catalysts." For example, your stomach would not be able to break down food if it did not have special enzymes to speed up the rate of digestion. Antibodies that protect you against disease are proteins. Mus ...
protein expression (pdf, en, 2701 KB, 4/8/10)
... in the enriched minimal medium Mixed protocol → the culture is grown in unlabeled rich medium (i.e. LB) and, right before induction, the cells are harvested, washed and resuspended in the enriched minimal medium ...
... in the enriched minimal medium Mixed protocol → the culture is grown in unlabeled rich medium (i.e. LB) and, right before induction, the cells are harvested, washed and resuspended in the enriched minimal medium ...
TRANSLASI - alanindra
... helped and guided in the folding process by chaperone proteins • Many proteins have sugars, phosphate groups, fatty acids, and other molecules covalently attached to certain amino acids. Most of this is done in the endoplasmic reticulum. • Many proteins are targeted to specific organelles within the ...
... helped and guided in the folding process by chaperone proteins • Many proteins have sugars, phosphate groups, fatty acids, and other molecules covalently attached to certain amino acids. Most of this is done in the endoplasmic reticulum. • Many proteins are targeted to specific organelles within the ...
10.3 Protein Synthesis
... • The language of mRNA is called the Genetic Code (A, G, U, C) (contains only 4 letters) • It is the matching of the RNA sequence to the correct amino acid to make proteins. • It is based on codons, which are 3 bases together on an mRNA chain. • Each codon codes for a specific amino acid • There a ...
... • The language of mRNA is called the Genetic Code (A, G, U, C) (contains only 4 letters) • It is the matching of the RNA sequence to the correct amino acid to make proteins. • It is based on codons, which are 3 bases together on an mRNA chain. • Each codon codes for a specific amino acid • There a ...
Additional file 1, Table S1. Summary of yeast two
... of the Hes6-like subfamily: the loops of Her13 and Hes6 contain 5 less amino acids when compared with other members, and the loops of Her8a and Her8.2 have 3 less amino acids (domain overlayed with the black bar). In addition, the Orange domains of Hes6-like proteins are 65-86% identical to each oth ...
... of the Hes6-like subfamily: the loops of Her13 and Hes6 contain 5 less amino acids when compared with other members, and the loops of Her8a and Her8.2 have 3 less amino acids (domain overlayed with the black bar). In addition, the Orange domains of Hes6-like proteins are 65-86% identical to each oth ...
Antibodies (Immunoglobulin)
... heavy chains (H) and two identical light chains (L), each with a variable region (V) & constant region (C). ...
... heavy chains (H) and two identical light chains (L), each with a variable region (V) & constant region (C). ...
File
... heavy chains (H) and two identical light chains (L), each with a variable region (V) & constant region (C). ...
... heavy chains (H) and two identical light chains (L), each with a variable region (V) & constant region (C). ...
Enzymes - TeacherWeb
... • Proteins (ex: enzymes) are made up of DIFFERENT amino acids sequences (orders) • Each amino acid has different functional groups (R groups) • Different R groups in active site allow enzyme to bind different substrates ...
... • Proteins (ex: enzymes) are made up of DIFFERENT amino acids sequences (orders) • Each amino acid has different functional groups (R groups) • Different R groups in active site allow enzyme to bind different substrates ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.