• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Chapter 9
Chapter 9

...  One of the most common methods uses DNA ...
What observations did Darwin make that lead him to the Theory of
What observations did Darwin make that lead him to the Theory of

... water. How do these lead to cohesion and adhesion? 3. Discuss transpiration in plants, in the context of the properties of water. What factors influence the rate of transpiration, and in what way? 4. What is the inherent conflict in the need for plants to take in CO2 and conserve water? 5. Make a ta ...
AMINO ACIDS, POLYPEPTIDES, AND PROTEINS
AMINO ACIDS, POLYPEPTIDES, AND PROTEINS

... composed of four subunits while myoglobin only contains one. Hemoglobin’s quaternary structure allows a more effective control of oxygen transport than is possible with ...
Biology Organic Molecules Notes
Biology Organic Molecules Notes

... V. Molecules of Life B.) Proteins 1.) Made of mostly C, H, O, and N 2.) Are long chains of amino acids  Joined together by peptide bonds  Dipeptide: two amino acids  Polypeptide: very long chain of amino acids  Proteins all have a different shape but are all globular ...
MARKER GENE TECHNOLOGIES, Inc
MARKER GENE TECHNOLOGIES, Inc

... Is RedView compatible with Southern ...
O. Ugur Sezerman Sabanci University MDBF 34056 Orhanli
O. Ugur Sezerman Sabanci University MDBF 34056 Orhanli

Biology 3 Winter 2009 First Exam
Biology 3 Winter 2009 First Exam

... While all three are triglycerides, they differ in the relative number of carbon to carbon double bonds in their fatty acid tails. Saturated fats have no carbon to carbon double bonds and are unkinked. Monounsaturated fats have a single carbon to carbon double bond in each of their fatty acid tails a ...
Coevolution analysis of viral sequences: from HBV - LCQB
Coevolution analysis of viral sequences: from HBV - LCQB

... HBV entry process, including the binding and membrane fusion mechanisms. We shall tackle the problem of the interactions among surface proteins, even for proteins where no structure is available. We shall reconstruct coevolution signals on independent analysis of geno ...
Proteins - (www.ramsey.k12.nj.us).
Proteins - (www.ramsey.k12.nj.us).

... These compounds all come from our foods and are needed by our bodies. ...
Proteins
Proteins

... An average of 16% (15.1% to 16.8%) nitrogen mass in protein is assumed to calculate the protein concentration Protein is subjected to heat and strong acid to break it down Steps involves protein precipitation (NPN remains in the supernatant) digestion with H2SO4 at 340OC in presence of ...
Cell Culture
Cell Culture

... The ratio of the number of bronchial epithelial cells with positive results for survivin in the nucleus or cytoplasm to the total number of bronchial epithelial cells was determined in blind fashion for six fields (three from the upper lobe and three from the lower lobe) for all groups of mice (n = ...
Cytochrome P450 3A4: The Impossible Protein
Cytochrome P450 3A4: The Impossible Protein

... to which of these works but, but both have larger ions that would not be as detrimental to the protein. The salts used have been potassium chloride and sodium chloride, both of these seem to work the same. There have been three protein preservatives, DTT, EDTA, and BME. Of these, BME seems to work t ...
File - western undergrad. by the students, for the students.
File - western undergrad. by the students, for the students.

... that polypeptide strands like side by side, and are held together by hydrogen bonds (see Fig. 4-10, p. 130), forming a very rigid structure. Again, the polypeptide N-H and C=O groups form hydrogen bonds to stabilize the structure, but unlike the  helix, these bonds are formed between neighbouring p ...
물리화학 소개
물리화학 소개

... Tiny Particles Flag Scarce Proteins One-two punch. New detection technique tows a protein into place magnetically, then signals its presence by releasing DNA. Their target was prostate-specific antigen (PSA), a protein that can indicate prostate cancer in men and that is also being investigated as a ...
Biochem01 - Amit Kessel Ph.D
Biochem01 - Amit Kessel Ph.D

... The membrane transport protein Na -K ATPase carries ...
Supporting Information Heim et al. 10.1073/pnas.1413018111
Supporting Information Heim et al. 10.1073/pnas.1413018111

... the protein (2) (Fig. S1A). All anti-P1 primary antibodies were diluted 1:500 in PBS-0.3% Tween 20 (PBST). After incubation with primary antibody, the membranes were washed with PBST and reacted with an HRP-labeled goat anti-mouse secondary antibody (MP Biomedicals) at a dilution of 1:1,000 in PBST ...
Slide 1
Slide 1

...  Proteins can carry intrinsically disordered regions  These regions can be predicted from sequence  Eukaryotic proteins are more disordered ...
- Wiley Online Library
- Wiley Online Library

... 2- and 6-day-old plants. There is a small but constant difference of MA16 level between the leaves and the roots, the latter having a lower level of the protein. No significant change in the levels of the protein were observed in tissues under water stress. Since during water stress MA16 mRNA was si ...
A1982NF37500001
A1982NF37500001

... impurities from serum albumin; namely, treatment with activated charcoal at low pH. Physical tests showed that the method did not denature the protein. [The SCI® indicates that this paper has been cited over 1,070 times since 1967.] ...
doc Final Exam 2003
doc Final Exam 2003

... 6. Assume you have a mutant mouse that lacks voltage gated sodium channel inactivation. What change do you expect in the form of the action potential (AP)? a) the AP would be much faster than usual. b) repolarization of the membrane after the AP would be extremely slow. c) the after hyperpolarizatio ...
Nutritional management in ebola haemorrhagic fever
Nutritional management in ebola haemorrhagic fever

slide - KOCSEA
slide - KOCSEA

...  A combined measure of density within each cluster and separability among clusters  Estimated by the ratio of the number of edges within a cluster (sub-graph) to the number of all edges starting from the nodes in the cluster (sub-graph)  Observes the average modularity of clusters with respect to ...
Part 2 - Latona
Part 2 - Latona

... IV. Transcription making RNA from DNA in the nucleus A. Initiation: RNA polymerase opens up a section(Gene) of the DNA strand which is signaled with the start codon. B. Elongation: RNA nucleotides move in to make a complementary copy from DNA ...
Disentangling factors of gene expression regulation in human
Disentangling factors of gene expression regulation in human

bodylogix.com gnc.ca bodylogix.com gnc.ca gnc.ca
bodylogix.com gnc.ca bodylogix.com gnc.ca gnc.ca

... Lean Muscle Meals RTDs are an excellent tasting liquid nutrition formula designed for endurance and recovery. Lean Muscle Meal RTDs are packed with 25g of protein, 3g of fiber, 27 vitamins and minerals – all only 170 calories. Lean Muscle Meal allows Crossfit athletes to get all the vitamins, nutrie ...
< 1 ... 370 371 372 373 374 375 376 377 378 ... 592 >

Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report