• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Final Presentations Abstract Book - MSOE Center for BioMolecular
Final Presentations Abstract Book - MSOE Center for BioMolecular

... attaches to the DNA, causing the DNA to unwind and thereby making the DNA accessible to other transcription factors. This results in the activation of target genes, which are responsible for the maintenance of pluripotency in the stem cells. Once sufficient stem cells have been produced, FOXD3 is tu ...
Chapter 1: Animal Agriculture
Chapter 1: Animal Agriculture

... practical nutrition, a total mineral value called “ash” is determined by burning a feed sample • Digestible carbohydrates - the estimation of digestible carbohydrates (called nitrogen-free extract) was historically calculated by subtracting all of the previous analytical results from 100% ...
Metals in Redox Biology - University of Nebraska–Lincoln
Metals in Redox Biology - University of Nebraska–Lincoln

... MOLECLUAR CHAPERONES FOR FE-S CLUSTER ASSEMBLY -Isc pathway : contains HscA and HscB proteins homologues of the DnaJ and DnaK molecular chaperones. -This interaction is enhanced by HscB, which can bind to both IscU and HscA, leading to a strong enhancement of the intrinsic HscA ATPase activity. -Hsc ...
SAM Teachers Guide - RI
SAM Teachers Guide - RI

... Highlight that the protein is made up of one long amino acid chain that is folded into a specific shape. Emphasize that representing a protein’s structure in different styles and colors can illustrate the different, important aspects of its structure. Students will likely need help and feedback on c ...
File
File

... Hemoglobin in red blood cells acts as a buffer by preventing a drop in pH of the blood. It is a tertiary protein that bonds to iron, which gives it its red hue when carrying oxygen. ...
Biophysics 101 Genomics and Computational Biology
Biophysics 101 Genomics and Computational Biology

... and prediction of transmembrane helices. Jayasinghe et al. Backbone constraint, identifies TM helices of membrane proteins with an accuracy greater than 99 %. (& energetics of salt-bridge formation. Falsely predicts 17 to 43 % of a set of soluble proteins to be MPs, depending upon the hydropathy sca ...
Proteins - e
Proteins - e

... acid bases across the cell membrane such that the cell can synthesize its proteins, carbohydrates and nucleic acids. They are also important in transporting important neurotransmitters back into the neuron that released them so that the neurotransmitters only have a limited period of activity. The m ...
gmo adv
gmo adv

... • Pöpping, Bert. “Are you ready for [a] Roundup?” Journal of Chemical Education, June 2001, 78: 752–756. Then, answer the following questions: 1. Figure 1 in the article shows a pair of scissors cutting out a gene of interest. What kind of enzyme do the scissors represent? What techniques might you ...
file1
file1

... Four very large families to test their results on - nucleotidyl cyclases - eukaryotic protein kinases - lactate/malate dehydrogenases - trypsin-like serine proteases ...
Nugget
Nugget

... Protein Helix Helix Bundle Protein The stability of enzymes catalysts for application in organic synthesis can be ...
Protein Folding I and II
Protein Folding I and II

... From Levinthal to pathways to funnels) (b) Serpin scenario shows a landscape with a deep kinetic trap on the left (A), which is easily accessible from the open conformations. Chain trapped in this deep local minima anneal to the global minimum (B, (B in the middle) only very slowly. This corresponds ...
Gene Ontology (GO)
Gene Ontology (GO)

... few years, different fitness functions and algorithms have been developed, and protein threading has become one of the most active fields in theoretical molecular biology. In all cases, the paradigm of homology modeling is followed with its three basic steps of identifying the structural template, c ...
lecture CH21 chem131pikul
lecture CH21 chem131pikul

... Enzymes are proteins that serve as biological catalysts for reactions in all living organisms. •  They increase the rate of a reaction (106 to 1012 times faster), but are unchanged themselves. •  Enzymes are very specific; each enzyme catalyzes a certain reaction or type of reaction only. •  The nam ...
A1993LD65600001
A1993LD65600001

... able to immunopurify enough T antigen from The ability of small viruses to cause cancer SV40-infected cells using crude tumour bearer has provided a potent model system with which sera to see the protein as a commassie stained to understand the human disease. It became band on a gel. This allowed us ...
Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... Your DNA, or deoxyribonucleic acid, contains the genes that determine who you are. How can this organic molecule control your characteristics? DNA contains instructions for all the proteins your body makes. Proteins, in turn, determine the structure and function of all your cells. What determines a ...
Gene Section BAD (BCL2-antagonist of cell death) Atlas of Genetics and Cytogenetics
Gene Section BAD (BCL2-antagonist of cell death) Atlas of Genetics and Cytogenetics

... promoter homolog; BBC2; BCL2L8 (Bcl-2-like 8 protein) HGNC (Hugo): BAD Location: 11q13.1 ...
Slide 1
Slide 1

... By Jonathan Haltiwanger and Teresa Leslie ...
Nerve activates contraction
Nerve activates contraction

... • A functional proteins consists of one or more polypeptides that have been precisely twisted, folded, and coiled into a unique shape. • It is the order of amino acids that determines what the three-dimensional conformation will be. ...
Question Report - FM Faculty Web Pages
Question Report - FM Faculty Web Pages

... presentation all of the above ...
Previous IB Exam Essay Questions: Basic Molecules, Proteins
Previous IB Exam Essay Questions: Basic Molecules, Proteins

... in feedback inhibition a (end) product binds to the enzyme end-product is a substance produced in last / later stage of a pathway modulator / inhibitor / effector / product binds at the allosteric site / site away from the active site binding causes the enzyme / active site to change shape substrate ...
Gene Section SEPT2 (septin 2) Atlas of Genetics and Cytogenetics
Gene Section SEPT2 (septin 2) Atlas of Genetics and Cytogenetics

... cell were performed. Oncogenesis Although the presently available data suggest that the involvement of septins in MLL-related leukemia is only related to their capacity to oligomerize, there is some evidence that altered expression of SEPT2 may underlie the development of aneuploidy. ...
Protein Tertiary and Quaternary Structure
Protein Tertiary and Quaternary Structure

Tertiary and Quaternary Structure
Tertiary and Quaternary Structure

... such supersecondary structures as coiled coils of α-helices, stacked β-sheets, βαβ elements, β-barrels, and β saddles. Define the terms domain and subunit as they relate to protein structure. Be able to recognize different domains in a ribbon diagram of a single polypeptide chain with 2 or more doma ...
COMPARATIVE MODELING AND MOLECULAR
COMPARATIVE MODELING AND MOLECULAR

... with another yeast species, Saccharomyces cerevisiae AspRS, the only protein molecule with its x-ray determined 3D crystal structure. This 3D AspRS was used as a template to guide the exploration of our homolog models. Since the sequence identity in both yeast AspRSs is high, it would be a better op ...
Document
Document

... -tertiary structure – folded shape of the polypeptide chain -quaternary structure – interactions between multiple polypeptide subunits Protein folding is aided by chaperone proteins. ...
< 1 ... 369 370 371 372 373 374 375 376 377 ... 592 >

Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report