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Trans-activation and DNA-binding properties of
Trans-activation and DNA-binding properties of

... assays were performed on COS-1 cells co-transfected with either GalO or G-Sox 18(160-255) and treated with either 8-bromo cAMP or okadaic acid. Results shown are the mean CAT activity and the SDs derived from experiments done at least in triplicate. ...
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Ch 3 Membrane Transports

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... Although the term lipid is sometimes used as a synonym for fats, fats are a subgroup of lipids called trilycerides. Lipids also encompass molecules such as fatty acids and their derivatives, as well as other sterolcontaining metabolites such as cholesterol . Although humans and other mammals use var ...
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... Select one of these amino acids and: i) Show, by the removal, addition, or replacement of a small group, such as CH3, OH, etc., (i.e. not the entire sidechain) how you could convert your chosen amino acids to another amino acid that is chemically most similar to the starting amino acid. For example: ...
Chemistry of Life Chapter 3 ORGANIC CHEMISTRY Introduction
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... c. Tertiary structure: refers to the further folding due to interactions among R groups along the polypeptide chain. d. Quaternary structure describes the complexing of two of more polypeptide chains to form globular (example: hemoglobin) or fibrous proteins. C. Protein Denaturation: Loss of Structu ...
secstruct_and_sign_pep_PT
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Seminar_3 - Great Lakes Genomics Center

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... maintaining the cellular redox state by regenerating reduced glutathione, which is critical for cellular protection against mitochondrial-derived reactive oxygen species (ROS). Consequently, TIGAR expression modulates p53-induced apoptosis in response to ROS-associated DNA damage. Since elevated lev ...
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Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
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