Cell Transport
... • Hypotonic – solution has a lower solute concentration than the cell. • Hypertonic – solution has a higher concentration of solute than the cell ...
... • Hypotonic – solution has a lower solute concentration than the cell. • Hypertonic – solution has a higher concentration of solute than the cell ...
1400145689.
... 1. (a)(i) What is the purpose of care labels put on ready-made clothes? (2mks) (ii) What information do they generally contain? (6mks) (b) (i) Draw at least six care labels and symbols (ii) Explain what the labels and symbols mean. 2. (a) (i) What is dry cleaning? ...
... 1. (a)(i) What is the purpose of care labels put on ready-made clothes? (2mks) (ii) What information do they generally contain? (6mks) (b) (i) Draw at least six care labels and symbols (ii) Explain what the labels and symbols mean. 2. (a) (i) What is dry cleaning? ...
SGPP Malaria protein expression vectors now
... SGPP Malaria protein expression vectors now available at MR4 We are pleased to announce that Plasmodium spp. His-tagged bacterial expression plasmid vectors developed as part of the Structural Genomics of Pathogenic Protozoa (SGPP) consortium are now available at MR4. Wim Hol at the University of Wa ...
... SGPP Malaria protein expression vectors now available at MR4 We are pleased to announce that Plasmodium spp. His-tagged bacterial expression plasmid vectors developed as part of the Structural Genomics of Pathogenic Protozoa (SGPP) consortium are now available at MR4. Wim Hol at the University of Wa ...
mnw2yr_lec1_2004
... cells of a human body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, and are called genes (1 gene codes for 1 protein in principle) • Human DNA contains ~30,000 expressed genes • Deoxyri ...
... cells of a human body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, and are called genes (1 gene codes for 1 protein in principle) • Human DNA contains ~30,000 expressed genes • Deoxyri ...
Information Retrieval Performance and Method
... using the list of AD-related genes/proteins derived earlier as queries, and to parse out drug terms in the retrieved articles later. Here, we particularly withhold the urge of expediently retrieving PubMed abstracts using a conventional query term such as “Alzheimer”. Instead, we built a PubMed quer ...
... using the list of AD-related genes/proteins derived earlier as queries, and to parse out drug terms in the retrieved articles later. Here, we particularly withhold the urge of expediently retrieving PubMed abstracts using a conventional query term such as “Alzheimer”. Instead, we built a PubMed quer ...
Macromolecules 2: Proteins and Nucleic Acids Amino Acids differ
... • Sometimes a single functional PROTEIN is made of several POLYPEPTIDES that work together as a unit ...
... • Sometimes a single functional PROTEIN is made of several POLYPEPTIDES that work together as a unit ...
Immunochemical methods in biochemistry
... The first antibody is in excess adsorbed to a solid phase. The serum sample or calibrators containing the desired antigen are added to the well with immobilized antibody incubation all the non-reacting material is washed away a second enzyme-labeled antibody (different from the first antibody) ...
... The first antibody is in excess adsorbed to a solid phase. The serum sample or calibrators containing the desired antigen are added to the well with immobilized antibody incubation all the non-reacting material is washed away a second enzyme-labeled antibody (different from the first antibody) ...
Unit 1 Ch. 1, 17, 18. WHAT IS BIOLOGY?
... b Initiation, the first stage of translating mRNA, will start when an initiator tRNA binds to a small ribosomal subunit. ...
... b Initiation, the first stage of translating mRNA, will start when an initiator tRNA binds to a small ribosomal subunit. ...
Yeast Chitin Binding Domain Tag | NEB
... lactis. The CBD tag permits rapid recovery of secreted fusion proteins using chitin resin or chitin magnetic beads. The CBD tag tightly and selectively binds chitin without the need for concentrating or buffer exchanging the growth medium. Elution from chitin occurs sharply upon brief exposure to a ...
... lactis. The CBD tag permits rapid recovery of secreted fusion proteins using chitin resin or chitin magnetic beads. The CBD tag tightly and selectively binds chitin without the need for concentrating or buffer exchanging the growth medium. Elution from chitin occurs sharply upon brief exposure to a ...
AP review
... Goal: to find protein with known structure which best matches a given sequence. Since similarity between target and the closest to it template is not high, sequence-sequence alignment methods fail. Solution: threading – sequence-structure alignment method. ...
... Goal: to find protein with known structure which best matches a given sequence. Since similarity between target and the closest to it template is not high, sequence-sequence alignment methods fail. Solution: threading – sequence-structure alignment method. ...
Protein Phosphorylation in Rhodomicrobium vmnielii
... [32P]orthophosphateat concentrations of 10-50 pCi m1-l. For phosphoamino acid analysis Rm. vannielii was grown in the same medium except that it lacked all phosphate apart from that provided by the 1 in 50 inoculum and contained 50 mM-HEPES pH 6.8. Homogeneous populations of swarmer cells were prepa ...
... [32P]orthophosphateat concentrations of 10-50 pCi m1-l. For phosphoamino acid analysis Rm. vannielii was grown in the same medium except that it lacked all phosphate apart from that provided by the 1 in 50 inoculum and contained 50 mM-HEPES pH 6.8. Homogeneous populations of swarmer cells were prepa ...
Chapter 3
... -tertiary structure – folded shape of the polypeptide chain -quaternary structure – interactions between multiple polypeptide subunits Protein folding is aided by chaperone proteins. ...
... -tertiary structure – folded shape of the polypeptide chain -quaternary structure – interactions between multiple polypeptide subunits Protein folding is aided by chaperone proteins. ...
Back Matter
... - - , staphylococcal, in germfree mice, 358 Antibody detection by isotope techniques, 829 determinations, production and effect, 793 excess, formation of soluble complexes in region of, 935 formation and phagocytosis, 228 - - - - in primary response, 60 - - , human measles, by mixed agglutination, 9 ...
... - - , staphylococcal, in germfree mice, 358 Antibody detection by isotope techniques, 829 determinations, production and effect, 793 excess, formation of soluble complexes in region of, 935 formation and phagocytosis, 228 - - - - in primary response, 60 - - , human measles, by mixed agglutination, 9 ...
Breakfast of Champions
... Whey comes in three general forms that include concentrate (WPC), isolate (WPI) and hydrolysate (WPH). Whey concentrates are relatively low in fat but have higher levels of carbohydrates and bioactive compounds. Whey isolates are treated to remove fat and lactose which lowers its bioactive compoun ...
... Whey comes in three general forms that include concentrate (WPC), isolate (WPI) and hydrolysate (WPH). Whey concentrates are relatively low in fat but have higher levels of carbohydrates and bioactive compounds. Whey isolates are treated to remove fat and lactose which lowers its bioactive compoun ...
Molecular Methods - Roswell Park Cancer Institute
... Take primer and plasmid to core facility Automatic fluorescent sequencing: Incorporation of dye-labeled ddNTPs into DNA extension products. Each dye emits a different wavelength when excited by argon ion laser so that all four bases are detectable in a single lane of the gel. ...
... Take primer and plasmid to core facility Automatic fluorescent sequencing: Incorporation of dye-labeled ddNTPs into DNA extension products. Each dye emits a different wavelength when excited by argon ion laser so that all four bases are detectable in a single lane of the gel. ...
Chapter 8
... These are then used in PCR to generate random mutations in our gene of interest that has been cloned into a plasmid. The left and right portions of the target DNA are amplified separately by PCR. The amplified fragments are amplified, denatured to make them single stranded, and then re-annealed. ...
... These are then used in PCR to generate random mutations in our gene of interest that has been cloned into a plasmid. The left and right portions of the target DNA are amplified separately by PCR. The amplified fragments are amplified, denatured to make them single stranded, and then re-annealed. ...
BIOCHEMISTRY - Mexico Central School District
... Enzymes (aid in chemical reactions): amylase and proteases ...
... Enzymes (aid in chemical reactions): amylase and proteases ...
Enzymes
... ENZYMES ARE SPECIFIC • Every enzyme can only be used for one reaction. Each one can only bond with one substrate • So every time you have a new substrate, you need a new enzyme • This is called being SUBSTRATE SPECIFIC ...
... ENZYMES ARE SPECIFIC • Every enzyme can only be used for one reaction. Each one can only bond with one substrate • So every time you have a new substrate, you need a new enzyme • This is called being SUBSTRATE SPECIFIC ...
The extracellular matrix (ECM)
... -sulfated glycoamino glycans and a protein core -cover huge areas of extracellular matrix - eg. Aggrecan in cartilage and other connective tissues -contains hyaluronic acid + link protein + core protein ...
... -sulfated glycoamino glycans and a protein core -cover huge areas of extracellular matrix - eg. Aggrecan in cartilage and other connective tissues -contains hyaluronic acid + link protein + core protein ...
Trans-activation and DNA-binding properties of
... assays were performed on COS-1 cells co-transfected with either GalO or G-Sox 18(160-255) and treated with either 8-bromo cAMP or okadaic acid. Results shown are the mean CAT activity and the SDs derived from experiments done at least in triplicate. ...
... assays were performed on COS-1 cells co-transfected with either GalO or G-Sox 18(160-255) and treated with either 8-bromo cAMP or okadaic acid. Results shown are the mean CAT activity and the SDs derived from experiments done at least in triplicate. ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.