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Cellular Control revision - Mrs Jones A
Cellular Control revision - Mrs Jones A

... Gene repression in Prokaryotes, structural genes are transcribed all the time,(1) and when the end product is present in excess to requirements the gene is switched off.(1) ...
Biochemistry
Biochemistry

... (c) Proteins of different molecular weight may be separated by SDS-PAGE. After electrophoresis the protein bands can be visualised by staining. Results from such an experiment are shown below, together with data from the electrophoresis of standard proteins of known molecular weight: Distance (cm) ...
Bio 263/F94/Final - Millersville University
Bio 263/F94/Final - Millersville University

... 59. __________ allow molecules with low molecular weights to pass between cells via a direct connection between their cytoplasms. The purpose of this connection is to allow communication between the cells so that they can _________. a. Plasmodesmata, act in concert c. Gap junctions, swell e. a and d ...
Introduction to Protein-protein Interaction
Introduction to Protein-protein Interaction

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SECTION D What Does DNA Do?
SECTION D What Does DNA Do?

... give structure and shape to living cells and that carry out all of the chemical reactions necessary for life. The importance of DNA is that it contains the information that is used to make all of the proteins on which life depends. The proteins whose structures are specified by our DNA do more than ...
Overview of Metaboli.. - Frozen Crocus Productions
Overview of Metaboli.. - Frozen Crocus Productions

... Glycolysis: produces pyruvate for acetyl CoA production in mitochondria, produces NADH (electrons) for ETC in mitochondria, anaerobic production of ATP MK & CPK: anaerobic production of ATP TCA: accepts acetyl-CoA for citrate synthesis, production of NADH (electrons) & TCAintermediates can be used f ...
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English 

... Pepsin breaks down long globular proteins into shorter polypeptide chains. PowerPoint Slide 5. B. After the stomach, the proteins are further digested in the small intestine by enzymes secreted by the pancreas. The pancreas releases the enzyme Trypsin that continues to break down proteins into amino ...
SILAC and iTRAQ Quantitation on an Orbitrap Using Protein
SILAC and iTRAQ Quantitation on an Orbitrap Using Protein

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Lecture 19 Membranes 2: Membrane Proteins
Lecture 19 Membranes 2: Membrane Proteins

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... Metabolism The sum of the chemical reactions that occur in a living organism. Micronutrient Nutrients needed in very small amounts, less than 0.005% of body mass per day. They are used to enable the body to produce enzymes and hormones. Monosaccharide Simple sugar with the formula CnH2nOn. They are ...
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... Formula FS-72 is the flag ship sports food of elite company ATHLETES ADVANTAGE PTY LTD. Exclusive line up of products .FS-72 is a natural food containing vitamins, minerals, proteins and omega 3 and 6. FS-72 can be used by beginners , athletes and body builders. FS-72 can be used for weight lose thr ...
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Identification of a New Protein Localized at Sites of Cell

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DNA Transcription & Protein Translation
DNA Transcription & Protein Translation

... code for proteins and determine traits. 2. To investigate and understand common mechanisms of protein synthesis. ...
Membrane Adaptation and Solute Uptake Systems
Membrane Adaptation and Solute Uptake Systems

... to identify the transmembrane α-helices. Structures can also be compared with those of some other types of membrane proteins from E. coli that have been studied extensively. However, in relation to transport proteins in general, bacteriorhodopsin is unusual in that it has 7 transmembrane α-helices, ...
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C h e m g u id e –... PROTEINS: ENZYME INHIBITORS

... 1. a) A competitive inhibitor is one which competes with the substrate for the active site on the enzyme, and gets in the way of the reaction you want. In this case, malonate ions have a similar shape to succinate ions, and the same groups to bind to the active site with. Once a malonate ion is atta ...
SHORT COMMUNICATION Why Are Both Ends of the Polypeptide
SHORT COMMUNICATION Why Are Both Ends of the Polypeptide

... into their native conformations after they have been denatured. Whether this is because they are not completely unfolded during denaturation or because they can fold completely without being guided by the gripping proposed here remains to be understood. This gripping will have a profound effect on t ...
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... using X-ray crystallography and NMR ...
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Publication JournalArticle (Originalarbeit in einer wissenschaftlichen
Publication JournalArticle (Originalarbeit in einer wissenschaftlichen

... Despite their highly diverse functions in vivo, HOX proteins display similar biochemical properties in vitro, raising the question of how this specificity is achieved. In our study, we investigated the importance of the Antennapedia (Antp) YPWM motif for homeotic transformations in adult Drosophila. ...
Enzyme Active Sites - Center for BioMolecular Modeling
Enzyme Active Sites - Center for BioMolecular Modeling

< 1 ... 363 364 365 366 367 368 369 370 371 ... 592 >

Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
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