Exam I Review - Iowa State University
... What type of transport protein can move 2 or more different molecules in opposite directions? a. Uniporter b. Antiporter c. Symporter d. Multiporter e. Diporter In the formation of salt, the chlorine atom– a. Gains an electron from sodium b. Becomes a positive ion c. Has one more proton than electro ...
... What type of transport protein can move 2 or more different molecules in opposite directions? a. Uniporter b. Antiporter c. Symporter d. Multiporter e. Diporter In the formation of salt, the chlorine atom– a. Gains an electron from sodium b. Becomes a positive ion c. Has one more proton than electro ...
Manual_AccuRapid™ Protein Synthesis Kit
... 6) Mix protein expression mixture and magnetic bead by pipetting and incubate at room temperature for 5 min. 7) Combine magnet plate with separation rack and remove supernatant from the bead. (* Supernatant from this step is Unbound sample.) 8) After separating magnet plate from separation rack, add ...
... 6) Mix protein expression mixture and magnetic bead by pipetting and incubate at room temperature for 5 min. 7) Combine magnet plate with separation rack and remove supernatant from the bead. (* Supernatant from this step is Unbound sample.) 8) After separating magnet plate from separation rack, add ...
Interaction of small* molecules with membranes.
... ¾ * impossible to distinguish among permeability of H+ and that of OH¾ Significant permeability (P = 10-4 - 10-8 cm/s) - 106 times greater than for other ions ¾ Large scatter ⇐ different experimental conditions: e.g. vesicle size, different pH gradient, lipid unsaturation ...
... ¾ * impossible to distinguish among permeability of H+ and that of OH¾ Significant permeability (P = 10-4 - 10-8 cm/s) - 106 times greater than for other ions ¾ Large scatter ⇐ different experimental conditions: e.g. vesicle size, different pH gradient, lipid unsaturation ...
Molecules of Life
... Molecules of Life What are organic molecules? Compounds that contain carbon What are biological molecules? ...
... Molecules of Life What are organic molecules? Compounds that contain carbon What are biological molecules? ...
Amino acids
... charged R groups, and hydrophobic interactions and van der Waals interactions among hydrophobic R groups. – While these three interactions are relatively weak, strong covalent bonds called disulfide bridges that form between the sulfhydryl groups (SH) of two cysteine monomers act to rivet parts of t ...
... charged R groups, and hydrophobic interactions and van der Waals interactions among hydrophobic R groups. – While these three interactions are relatively weak, strong covalent bonds called disulfide bridges that form between the sulfhydryl groups (SH) of two cysteine monomers act to rivet parts of t ...
FYVE-dependent endosomal targeting of an arrestin-related
... same buffer, Laemmli denaturating gel buffer was added directly to the beads and the proteins were analyzed by Western blot using the anti-Arf antibody. ...
... same buffer, Laemmli denaturating gel buffer was added directly to the beads and the proteins were analyzed by Western blot using the anti-Arf antibody. ...
Application Note #2 - GE Healthcare Life Sciences
... the Multiphor II electrophoresis unit and pre-cooled to 16 °C. The glass backed cellulose TLC plates were marked with a blunt soft pencil at 3 and 9 cm for the origin and migration point for the bromophenol blue tracking dye, respectively. Phosphoamino Acid Standards (1 µ1, ~25 nmol of each phosphoa ...
... the Multiphor II electrophoresis unit and pre-cooled to 16 °C. The glass backed cellulose TLC plates were marked with a blunt soft pencil at 3 and 9 cm for the origin and migration point for the bromophenol blue tracking dye, respectively. Phosphoamino Acid Standards (1 µ1, ~25 nmol of each phosphoa ...
Follow Monty Python's Footsteps: Towards the Holy Grail of Protein Structure Prediction
... Beta strands that are completely buried usually contain a run of hydrophobic residues, since both faces are buried in the protein core. This strand from Chemotaxis protein CheY is a good example: ...
... Beta strands that are completely buried usually contain a run of hydrophobic residues, since both faces are buried in the protein core. This strand from Chemotaxis protein CheY is a good example: ...
Computational Protein Design as a Cost Function Network
... of protein folding [28]: the three-dimensional structure is known and we have to find amino acid sequences that folds into it. It can also be considered as a highly combinatorial variant of side-chain positioning [35] because of possible amino acid changes. Different computational methods have been ...
... of protein folding [28]: the three-dimensional structure is known and we have to find amino acid sequences that folds into it. It can also be considered as a highly combinatorial variant of side-chain positioning [35] because of possible amino acid changes. Different computational methods have been ...
Pdf file
... • Mitochondria contain their own genome and protein synthe3c machinery (tRNAs, mRNAs, ribosomes, ini3a3on and elonga3on factors, etc.) • Mitochondria are comprised of hundreds of dis3nct proteins, only a handful of which are encoded in the mitochondrial genome (varies by species) • Most mi ...
... • Mitochondria contain their own genome and protein synthe3c machinery (tRNAs, mRNAs, ribosomes, ini3a3on and elonga3on factors, etc.) • Mitochondria are comprised of hundreds of dis3nct proteins, only a handful of which are encoded in the mitochondrial genome (varies by species) • Most mi ...
Optimization of Ammonium Sulfate Concentration for Purification of
... and easy homogenization processing for protein purification due to soft leaf tissue (Jamal et al., 2009, 2012; Song et al., 2015). The tumor-associated antigen GA733, a glycoprotein that is highly expressed on the cell-surface of colorectal carcinomas, has previously been fused with the human immunog ...
... and easy homogenization processing for protein purification due to soft leaf tissue (Jamal et al., 2009, 2012; Song et al., 2015). The tumor-associated antigen GA733, a glycoprotein that is highly expressed on the cell-surface of colorectal carcinomas, has previously been fused with the human immunog ...
Macromolecule Jeopardy
... 500- Nucleic acids contain the instructions on how to make which other type of macromolecule? Proteins Grab Bag 100- What elements have to be in a molecule for it to be considered organic? Carbon and hydrogen 200- Is a saturated fat solid or liquid at room temperature? solid 300- What are is the dif ...
... 500- Nucleic acids contain the instructions on how to make which other type of macromolecule? Proteins Grab Bag 100- What elements have to be in a molecule for it to be considered organic? Carbon and hydrogen 200- Is a saturated fat solid or liquid at room temperature? solid 300- What are is the dif ...
FYVE-Dependent Endosomal Targeting of an Arrestin
... same buffer, Laemmli denaturating gel buffer was added directly to the beads and the proteins were analyzed by Western blot using the anti-Arf antibody. ...
... same buffer, Laemmli denaturating gel buffer was added directly to the beads and the proteins were analyzed by Western blot using the anti-Arf antibody. ...
Polyacrylamide Gel Electrophoresis Analysis of Ribosomal Protein
... rpm) at 30°C. After cultivation for 20 to 30 h (mid-exponential phase), the cells were collected, washed, and then disrupted by sonication for 3 to 5 min, as described previously (19). The cells of several Actinomadura species were hardly disrupted by sonic treatment, but the cells were not treated ...
... rpm) at 30°C. After cultivation for 20 to 30 h (mid-exponential phase), the cells were collected, washed, and then disrupted by sonication for 3 to 5 min, as described previously (19). The cells of several Actinomadura species were hardly disrupted by sonic treatment, but the cells were not treated ...
The QIAexpressionist™
... the tag is small, uncharged, and therefore does not generally affect secretion, compartmentalization, or folding of the fusion protein within the cell. In most cases, the 6xHis tag does not interfere with the structure or function of the purified protein as demonstrated for a wide variety of protein ...
... the tag is small, uncharged, and therefore does not generally affect secretion, compartmentalization, or folding of the fusion protein within the cell. In most cases, the 6xHis tag does not interfere with the structure or function of the purified protein as demonstrated for a wide variety of protein ...
Protein expression, purification, and molecular cloning
... 1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is the technique used to separate proteins based upon molecular weight. Polyacrylamide is a polymer that is used to separate proteins and small DNA and RNA fragments (<100bp). While polyacrylamide is nontoxic, acrylam ...
... 1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is the technique used to separate proteins based upon molecular weight. Polyacrylamide is a polymer that is used to separate proteins and small DNA and RNA fragments (<100bp). While polyacrylamide is nontoxic, acrylam ...
Journal of Bacteriology
... with 11% acrylamide gels (16). Suspensions of either lysozyme-treated or nontreated cell envelopes were mixed with concentrated sample buffer and incubated at the appropriate temperature for 10 min before electrophoresis. Separated cell envelope constituents were transferred from gels to nitrocellul ...
... with 11% acrylamide gels (16). Suspensions of either lysozyme-treated or nontreated cell envelopes were mixed with concentrated sample buffer and incubated at the appropriate temperature for 10 min before electrophoresis. Separated cell envelope constituents were transferred from gels to nitrocellul ...
Prediction - Center for Biological Sequence Analysis
... • Define a threshold for being ”neighbours” (too closely related) • Calculate numbers of neighbours for each example, and remove the example with most neighbours • Repeat until there are no examples with neighbours left Alternative: Homology partitioning • keep all examples, but cluster them so that ...
... • Define a threshold for being ”neighbours” (too closely related) • Calculate numbers of neighbours for each example, and remove the example with most neighbours • Repeat until there are no examples with neighbours left Alternative: Homology partitioning • keep all examples, but cluster them so that ...
Modeling with Toobers
... Single domain of zinc finger domain with the N-terminal end at the top right and C-terminus at the bottom right. ...
... Single domain of zinc finger domain with the N-terminal end at the top right and C-terminus at the bottom right. ...
Proteins - Cook Biology
... Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells. • Protein functions include structural support, storage, transport, cellular communications, movement, defense against foreign substances, and organic catalysts ...
... Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells. • Protein functions include structural support, storage, transport, cellular communications, movement, defense against foreign substances, and organic catalysts ...
Amino acids and prot..
... hooves, or soft, as is the case with skin. It is difficult to dissolve keratin because it contains non polar amino acids plus cysteine, which means that it is able to form disulfide bridges. These bridges create a thread shape that is extremely strong, as sulfur atoms bond to each other, creating a ...
... hooves, or soft, as is the case with skin. It is difficult to dissolve keratin because it contains non polar amino acids plus cysteine, which means that it is able to form disulfide bridges. These bridges create a thread shape that is extremely strong, as sulfur atoms bond to each other, creating a ...
Advanced techniques yield new insights into ribosome selfassembly
... assembled,” she said. “But it doesn’t tell you anything about how it gets there, how all these parts come together.” All ribosomes consist of two subunits, each a cluster of precisely folded proteins and RNA. The team focused on the small ribosomal subunit of the E. coli bacterium. It is made up of ...
... assembled,” she said. “But it doesn’t tell you anything about how it gets there, how all these parts come together.” All ribosomes consist of two subunits, each a cluster of precisely folded proteins and RNA. The team focused on the small ribosomal subunit of the E. coli bacterium. It is made up of ...
Lh6Ch03cProtSeq
... – Can be used to determine post-translational modifications. – Actual sequence can also be determined from DNA sequence ...
... – Can be used to determine post-translational modifications. – Actual sequence can also be determined from DNA sequence ...
Cloning of a cDNA Encoding a Plasma Membrane
... pectin (G-50 PIIF; Bishop et al., 1984). These uronides were labeled at the reducing ends with biotin and purified by affinity chromatographyon avidin columns to remove unlabeled uronides (see Methods). The number of biotin molecules per uronide was not quantitated, but it is likely to be one becaus ...
... pectin (G-50 PIIF; Bishop et al., 1984). These uronides were labeled at the reducing ends with biotin and purified by affinity chromatographyon avidin columns to remove unlabeled uronides (see Methods). The number of biotin molecules per uronide was not quantitated, but it is likely to be one becaus ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.