A parafusin-related Toxoplasma protein in Ca -regulated secretory organelles
... VT/USA). Images were acquired using a FKI1001 digital CCD camera (PerkinElmer Life Sciences, Boston, MA/USA) using Esprit software (PerkinElmer Life Sciences). Deconvolution imaging was performed using a Provis AX70 microscope (Olympus) with a PlanApo 60 , 1.4 NA objective (Olympus) and High-Q fil ...
... VT/USA). Images were acquired using a FKI1001 digital CCD camera (PerkinElmer Life Sciences, Boston, MA/USA) using Esprit software (PerkinElmer Life Sciences). Deconvolution imaging was performed using a Provis AX70 microscope (Olympus) with a PlanApo 60 , 1.4 NA objective (Olympus) and High-Q fil ...
A simple calculus for proteins and cells
... some reactions and disable other ones. The second feature is about the semantics. Process calculi have models that have been thoroughly studied and that support automatic verification tools. These models may turn out essential in order to formalise the behaviour of biological systems (with qualitat ...
... some reactions and disable other ones. The second feature is about the semantics. Process calculi have models that have been thoroughly studied and that support automatic verification tools. These models may turn out essential in order to formalise the behaviour of biological systems (with qualitat ...
View/Download
... And NuAquos, from New Whey Nutrition, contains a propriety blend of protein, electrolytes, vitamins and minerals. With 12 grams of protein, it promotes recovery and rehydration. “We think one of the problems protein’s had in the past is they haven’t been able to make a good tasting product,” said Ch ...
... And NuAquos, from New Whey Nutrition, contains a propriety blend of protein, electrolytes, vitamins and minerals. With 12 grams of protein, it promotes recovery and rehydration. “We think one of the problems protein’s had in the past is they haven’t been able to make a good tasting product,” said Ch ...
ITC - University of Victoria
... calorimeters became available. Evolved from a specialist method to a widely used technique ...
... calorimeters became available. Evolved from a specialist method to a widely used technique ...
A1981KX02600001
... As 32 P can be detected at very low concentrations by radioautography and assayed by count-ing techniques, 32P-labelled nucleic acids have been used in most subsequent studies on sequences of RNA and DNA. “In general, nucleotides do not fractionate well by ‘paper’ methods and we spent a good deal of ...
... As 32 P can be detected at very low concentrations by radioautography and assayed by count-ing techniques, 32P-labelled nucleic acids have been used in most subsequent studies on sequences of RNA and DNA. “In general, nucleotides do not fractionate well by ‘paper’ methods and we spent a good deal of ...
The novel functions of the cytochrome b561 protein family in
... members are studied in detail for their physiological functions but most of them are not well understood. In the present study, we focused on a nematode C. elegans as the most suitable model. C. elegans contains 7 b561 homologs (Ceb561-1 to 7) but their functions are not studied. We chose Ceb561-1 a ...
... members are studied in detail for their physiological functions but most of them are not well understood. In the present study, we focused on a nematode C. elegans as the most suitable model. C. elegans contains 7 b561 homologs (Ceb561-1 to 7) but their functions are not studied. We chose Ceb561-1 a ...
Cellular Gate Technology
... 2.1 Making Proteins within a Cell Proteins are ordered molecular polymers of 50-1000 amino acids, of 20 different types. Each of the approximately 500-10,000 protein types in a typical cell consists of a unique sequence of amino acids. Moreover, each protein chain folds into a characteristic threedi ...
... 2.1 Making Proteins within a Cell Proteins are ordered molecular polymers of 50-1000 amino acids, of 20 different types. Each of the approximately 500-10,000 protein types in a typical cell consists of a unique sequence of amino acids. Moreover, each protein chain folds into a characteristic threedi ...
Crystallization Laboratory - UCLA
... Heavy atoms are useful because they are electron dense. Bottom of periodic table. High electron density is useful because X-rays are diffracted from electrons. When the heavy atom is bound to discrete sites in a protein crystal (a derivative), it alters the X-ray diffraction pattern slightly. Compar ...
... Heavy atoms are useful because they are electron dense. Bottom of periodic table. High electron density is useful because X-rays are diffracted from electrons. When the heavy atom is bound to discrete sites in a protein crystal (a derivative), it alters the X-ray diffraction pattern slightly. Compar ...
INF380 – Proteomics
... – A protein does not have one defined mass, but rather a mass distribution. This is due to the occurrence of stable isotopes. Due to their long sequence, proteins will have a much more complex mass distribution than the much shorter peptides. As a result, it is a lot easier to obtain a single, monoi ...
... – A protein does not have one defined mass, but rather a mass distribution. This is due to the occurrence of stable isotopes. Due to their long sequence, proteins will have a much more complex mass distribution than the much shorter peptides. As a result, it is a lot easier to obtain a single, monoi ...
tong-zhang-university-of-florida
... From raw MS data to Trx h target proteins Search the raw files against protein database using SEQUEST algorithm Filter the high confident peptides using 1% FDR as cutoff Normalization of the six quantification channels ...
... From raw MS data to Trx h target proteins Search the raw files against protein database using SEQUEST algorithm Filter the high confident peptides using 1% FDR as cutoff Normalization of the six quantification channels ...
Sample abstract
... various fish species (e.g. salmonids and cod). The exoprotease, AsaP1, is one of its main virulence factors, which induces all the pathology of atypical furunculosis when it is injected in its pure form into salmon. The AsaP1 encoding gene has been cloned and expressed in E. coli and an AsaP1 negati ...
... various fish species (e.g. salmonids and cod). The exoprotease, AsaP1, is one of its main virulence factors, which induces all the pathology of atypical furunculosis when it is injected in its pure form into salmon. The AsaP1 encoding gene has been cloned and expressed in E. coli and an AsaP1 negati ...
Designing Molecular Probes for the Detection of Gene Expression
... Site-Specific IgG-Nanoparticle Conjugations • EPL-Click can be combined with the incorporation of unnatural amino acids to allow for the site-specific attachment of IgG to nanoparticles. ...
... Site-Specific IgG-Nanoparticle Conjugations • EPL-Click can be combined with the incorporation of unnatural amino acids to allow for the site-specific attachment of IgG to nanoparticles. ...
Equilibrium and Free Energy of Protein Denaturation
... The denaturation of some proteins can be described by a two-state transition model in which the protein exists in either the native (N) or completely unfolded, denatured (D) conformation. In large and more complex proteins, there may be multiple unfolding intermediates where only part of the protein ...
... The denaturation of some proteins can be described by a two-state transition model in which the protein exists in either the native (N) or completely unfolded, denatured (D) conformation. In large and more complex proteins, there may be multiple unfolding intermediates where only part of the protein ...
Review Sheet - Phillips Scientific Methods
... between them) o Secondary Structure Hydrogen bonding at regular intervals Shapes include coils (alpha helix) Folds (beta sheet) Beta sheet has more hydrogen bonds An enzyme with beta sheets would be more difficult to denature Alpha helixes are easier to denature Structural properties d ...
... between them) o Secondary Structure Hydrogen bonding at regular intervals Shapes include coils (alpha helix) Folds (beta sheet) Beta sheet has more hydrogen bonds An enzyme with beta sheets would be more difficult to denature Alpha helixes are easier to denature Structural properties d ...
protein synthesis
... LIMITED LICENSE TO MODIFY. These PowerPoint® slides may be modified only by teachers currently teaching the Science and Global Issues SEPUP course to customize the unit to match their students’ learning levels or to insert additional teaching aides. Modified slides may be used only by the modifying ...
... LIMITED LICENSE TO MODIFY. These PowerPoint® slides may be modified only by teachers currently teaching the Science and Global Issues SEPUP course to customize the unit to match their students’ learning levels or to insert additional teaching aides. Modified slides may be used only by the modifying ...
Side-chain hydrophobicity scale derived from transmembrane
... the guest amino acids, we adapted soluble protein chemical denaturation methods to measure the stability of folded proteins using tryptophan fluorescence spectroscopy (18, 19). This approach has only previously been attempted with two other transmembrane proteins folded into lipid vesicles (20–23) a ...
... the guest amino acids, we adapted soluble protein chemical denaturation methods to measure the stability of folded proteins using tryptophan fluorescence spectroscopy (18, 19). This approach has only previously been attempted with two other transmembrane proteins folded into lipid vesicles (20–23) a ...
Introduction to Protein Labeling
... • Expression systems are based on the insertion of a gene into a host cell for its translation and expression into protein . • Many recombinant proteins can be expressed to high levels in E. coli systems. most common choice for expressing labeled proteins for NMR • Yeast (Pichia pastoris, Saccarom ...
... • Expression systems are based on the insertion of a gene into a host cell for its translation and expression into protein . • Many recombinant proteins can be expressed to high levels in E. coli systems. most common choice for expressing labeled proteins for NMR • Yeast (Pichia pastoris, Saccarom ...
Slide 1
... DNA is made of nitrogenous bases adenine, guanine, cytosine and thymine. RNA is made of nitrogenous bases adenine, guanine, cytosine and uracil. GENE is a piece of DNA capable of forming a functional product either protein or RNA. 5. Every cell typically has thousands of genes. 6. RNA is of 3 major ...
... DNA is made of nitrogenous bases adenine, guanine, cytosine and thymine. RNA is made of nitrogenous bases adenine, guanine, cytosine and uracil. GENE is a piece of DNA capable of forming a functional product either protein or RNA. 5. Every cell typically has thousands of genes. 6. RNA is of 3 major ...
Secondary Drug Resistance Mutation of TEM-1
... binding and catalysis (R164, G238, and E104) or inhibitor binding (M69 and R244) are repeatedly found. M182T does not have a direct role in catalysis or substrate/inhibitor binding and is never found alone. In the 3D structure, Met182 is far away from the sites of the coupled primary mutation so t ...
... binding and catalysis (R164, G238, and E104) or inhibitor binding (M69 and R244) are repeatedly found. M182T does not have a direct role in catalysis or substrate/inhibitor binding and is never found alone. In the 3D structure, Met182 is far away from the sites of the coupled primary mutation so t ...
lecture09_09
... How can we view the protein structure ? • Download the coordinates of the structure from the PDB ...
... How can we view the protein structure ? • Download the coordinates of the structure from the PDB ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.