Polymer Principles
... – An enzyme is specific in its action. – Example: Amylase changes the substrate starch into maltose. This chemical change occurs when starch is digested. – After the product is formed and released, the enzyme amylase can combine with another substrate molecule and change it into a product. – Starch ...
... – An enzyme is specific in its action. – Example: Amylase changes the substrate starch into maltose. This chemical change occurs when starch is digested. – After the product is formed and released, the enzyme amylase can combine with another substrate molecule and change it into a product. – Starch ...
Prediction of protein disorder: basic concepts and practical hints
... Partial unfolding enables the phosphorylation of Tyr88, starting a series of signaling events that leads to the beginning of S phase. ...
... Partial unfolding enables the phosphorylation of Tyr88, starting a series of signaling events that leads to the beginning of S phase. ...
Protein Synthesis
... basis for the major structural components of animal and human tissue. They also make up many of the elements that are necessary for the body to function, such as enzymes and antibodies. Some proteins are made in the cells of the body, while others can only be taken in from food. ...
... basis for the major structural components of animal and human tissue. They also make up many of the elements that are necessary for the body to function, such as enzymes and antibodies. Some proteins are made in the cells of the body, while others can only be taken in from food. ...
Preparation of enzymatically active recombinant class III
... • Class III HDACs can be readily purified as enzymatically active protein after overexpression in E. coli, generating a large amount of enzymatic deacetylase activity. • It has some limitations including the possible absence of regulatory cofactors and post-translation modifications. ...
... • Class III HDACs can be readily purified as enzymatically active protein after overexpression in E. coli, generating a large amount of enzymatic deacetylase activity. • It has some limitations including the possible absence of regulatory cofactors and post-translation modifications. ...
Unit 3: Chapter 6
... - Carry ____________ _______________ - _____________ for _________________ - The order of nitrogenous bases (A, T, G, C) determines the _______ of _____________ - The order of amino acids determines the protein ...
... - Carry ____________ _______________ - _____________ for _________________ - The order of nitrogenous bases (A, T, G, C) determines the _______ of _____________ - The order of amino acids determines the protein ...
Proteins
... The instructions for arranging amino acids into many different proteins are stored in DNA. Protein Molecule ...
... The instructions for arranging amino acids into many different proteins are stored in DNA. Protein Molecule ...
Iron Sulfur Proteins and their Synthetic Analogues: Structure
... 2Fe-2S centre are in the ferric state. It is difficult to assign formal oxidation states to the iron atoms in the reduced state. Ferredoxins may thus be regarded as a delocalised complex of iron with sulfur ligands. The unpaired electron in reduced ferredoxin can be regarded as being localised large ...
... 2Fe-2S centre are in the ferric state. It is difficult to assign formal oxidation states to the iron atoms in the reduced state. Ferredoxins may thus be regarded as a delocalised complex of iron with sulfur ligands. The unpaired electron in reduced ferredoxin can be regarded as being localised large ...
Lesson20ProteinStructureSearches
... Homology based protein conformation enhanced by making better decisions on where to insert gaps/varying length loops ...
... Homology based protein conformation enhanced by making better decisions on where to insert gaps/varying length loops ...
Mining the Human Genome Using Protein Structure Homology
... As structure representatives grow, so will ability y of remote homology gy detection Utilize fast fast, automated methods for assigning structure family ...
... As structure representatives grow, so will ability y of remote homology gy detection Utilize fast fast, automated methods for assigning structure family ...
Bioinorganic Chemistry of Metal
... variety of functions including oxygen storage/transport, elec tron transfer, redox catalysis with various substrates. Besides these traditional functions of hemeproteins, a new function of hemeprotein has been found recently, which is a sensor of diatomic gas molecules or redox change. 1) In these ...
... variety of functions including oxygen storage/transport, elec tron transfer, redox catalysis with various substrates. Besides these traditional functions of hemeproteins, a new function of hemeprotein has been found recently, which is a sensor of diatomic gas molecules or redox change. 1) In these ...
Chapter 5: What are the major types of organic molecules?
... 3. tertiary structure (3) of a protein is the overall folded shape of a single polypeptide chain, determined by secondary structure combined with interactions between R groups (NOTE: book defines this in a confusing way, use my way) 4. quaternary structure (4) of a protein results from interaction ...
... 3. tertiary structure (3) of a protein is the overall folded shape of a single polypeptide chain, determined by secondary structure combined with interactions between R groups (NOTE: book defines this in a confusing way, use my way) 4. quaternary structure (4) of a protein results from interaction ...
Carbohydrates
... Galactose: a sugar found in milk. It is a mirror image of glucose These three molecules are isomers. They have the same chemical formula but very different structures. Because they have the same formula, they can be converted easily from one form to another. ...
... Galactose: a sugar found in milk. It is a mirror image of glucose These three molecules are isomers. They have the same chemical formula but very different structures. Because they have the same formula, they can be converted easily from one form to another. ...
Data Sheet
... renal proximal tubules lead to urinary loss of cystine and basic amino acids. The BAT1 and rBAT proteins were shown to be colocalized in the apical membrane of the renal proximal tubules where massive cystine transport had been proposed, and BAT1 is associated with rBAT to express its function. This ...
... renal proximal tubules lead to urinary loss of cystine and basic amino acids. The BAT1 and rBAT proteins were shown to be colocalized in the apical membrane of the renal proximal tubules where massive cystine transport had been proposed, and BAT1 is associated with rBAT to express its function. This ...
SOPs - doaba college, jalandhar
... protein complexes. Polyacrylamide, which is easy to handle and to make at higher concentrations, is used to separate most proteins and small oligonucleotides that require a small gel pore size for retardation. Polyacrylamide is a cross-linked polymer of acrylamide. The length of the polymer chains i ...
... protein complexes. Polyacrylamide, which is easy to handle and to make at higher concentrations, is used to separate most proteins and small oligonucleotides that require a small gel pore size for retardation. Polyacrylamide is a cross-linked polymer of acrylamide. The length of the polymer chains i ...
condensation reaction
... DURING THE FORMATION OF A FAT, ENZYMECATALYZED CONDENSATION REACTIONS LINK GLYCEROL TO FATTY ACIDS BY AN ESTER LINKAGE, a bond between a hydroxyl group and a ...
... DURING THE FORMATION OF A FAT, ENZYMECATALYZED CONDENSATION REACTIONS LINK GLYCEROL TO FATTY ACIDS BY AN ESTER LINKAGE, a bond between a hydroxyl group and a ...
Lecture 13
... rapid method for identification of transgenic plants in the seedling stage and requires only a small amount of plant tissue. ...
... rapid method for identification of transgenic plants in the seedling stage and requires only a small amount of plant tissue. ...
PDF file
... The position of phosphate was determined by treating the substrate with CIP (data not shown). Asterisks indicate location of 32P label. (B) Effect of concentration of divalent cation on NTPase and RTPase activities. Immunoprecipitated PfRTH1 protein was incubated with: left panel, [g-32P]ATP-termina ...
... The position of phosphate was determined by treating the substrate with CIP (data not shown). Asterisks indicate location of 32P label. (B) Effect of concentration of divalent cation on NTPase and RTPase activities. Immunoprecipitated PfRTH1 protein was incubated with: left panel, [g-32P]ATP-termina ...
Protein Motif Analysis
... a sequence LOGO representing the conserved motif each position in the LOGO diagram shows the amino acids that are observed to occur in that position. the height of the letter is proportional to how many times that amino acid was observed in that position. The LOGO diagram above reflects the fact tha ...
... a sequence LOGO representing the conserved motif each position in the LOGO diagram shows the amino acids that are observed to occur in that position. the height of the letter is proportional to how many times that amino acid was observed in that position. The LOGO diagram above reflects the fact tha ...
Cell Communication
... binds to the a-adrenergic receptor on vascular smooth muscle cells. One approach to treating high blood pressures is to administer competitive inhibitors that bind to the a-adrenergic receptor. The Kd for binding of epinephrine to this receptor is ~0.6 mM. Which of the following compounds might be g ...
... binds to the a-adrenergic receptor on vascular smooth muscle cells. One approach to treating high blood pressures is to administer competitive inhibitors that bind to the a-adrenergic receptor. The Kd for binding of epinephrine to this receptor is ~0.6 mM. Which of the following compounds might be g ...
Acid/Bases Review
... Amino acids are linked together by peptide bonds (covalent bonds) • Polypeptide- more than 2 amino acids bonded together ...
... Amino acids are linked together by peptide bonds (covalent bonds) • Polypeptide- more than 2 amino acids bonded together ...
Measles virus M and F proteins associate with detergent
... equivalent to those described (Fig. 1d) were also detectable, yet neither of these protein species associated preferentially with the DRM or the detergent-soluble fractions. To investigate whether co-expression of F protein would alter DRM association of M proteins, pCG-M(ED) was transfected into 29 ...
... equivalent to those described (Fig. 1d) were also detectable, yet neither of these protein species associated preferentially with the DRM or the detergent-soluble fractions. To investigate whether co-expression of F protein would alter DRM association of M proteins, pCG-M(ED) was transfected into 29 ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.