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... 1.6.1 The hemoglobin molecule from sickle-cell anemia patients (HbS) was found to have a higher pI value (having more net positive charges). 1.6.2 Peptide fingerprinting (protease digestion + electrophoresis + chromatography) of HbS and HbA (wt) revealed that all but one of the peptide spots matched ...
... 1.6.1 The hemoglobin molecule from sickle-cell anemia patients (HbS) was found to have a higher pI value (having more net positive charges). 1.6.2 Peptide fingerprinting (protease digestion + electrophoresis + chromatography) of HbS and HbA (wt) revealed that all but one of the peptide spots matched ...
two genes control a superoxide stress regulon
... regulon. These genes, named soxR and soxS, are arranged divergently with their 5' ends separated by only 85 bp. The predicted 12.9-kDa SoxS protein is related to the AraC family of one-component gene regulators, but corresponds only to the putative DNAbinding regions of these proteins. The 17.1-kDa ...
... regulon. These genes, named soxR and soxS, are arranged divergently with their 5' ends separated by only 85 bp. The predicted 12.9-kDa SoxS protein is related to the AraC family of one-component gene regulators, but corresponds only to the putative DNAbinding regions of these proteins. The 17.1-kDa ...
Handout12B
... This handout is meant to provide some cultural background to the story told in the text book about determinants. As the text explained, the determinant of a square matrix is non-zero if and only if the matrix is invertible. The fact that the determinant signals invertibility is one of its prime uses ...
... This handout is meant to provide some cultural background to the story told in the text book about determinants. As the text explained, the determinant of a square matrix is non-zero if and only if the matrix is invertible. The fact that the determinant signals invertibility is one of its prime uses ...
ProteinStructurePredictionTalk
... – The global three-dimensional structure. – Defined in atomic coordinates. – The actual function. ...
... – The global three-dimensional structure. – Defined in atomic coordinates. – The actual function. ...
Description of PAOL (Protein Analysis On Line) ε N n k 4 =
... The degasser “SHIMADZU DGU 20A” enables to degas the -previously filtered at 0.1µm- column solvents. The pump “SHIMADZU LC-20AD” enables to generate the solvent flow through the different components of PAOL. The flow is characterized by a constant flow rate (typically 0.5 ml/min) and very low pulsat ...
... The degasser “SHIMADZU DGU 20A” enables to degas the -previously filtered at 0.1µm- column solvents. The pump “SHIMADZU LC-20AD” enables to generate the solvent flow through the different components of PAOL. The flow is characterized by a constant flow rate (typically 0.5 ml/min) and very low pulsat ...
Publications
... absorption, drug transport, storage, metabolism and excretion properties of typical drugs in vertebrates. Serum albumins are most plentiful proteins in the circulatory system of a wide variety of organisms. Among the serum albumins, bovine serum albumin (BSA) is a striking macromolecule often used i ...
... absorption, drug transport, storage, metabolism and excretion properties of typical drugs in vertebrates. Serum albumins are most plentiful proteins in the circulatory system of a wide variety of organisms. Among the serum albumins, bovine serum albumin (BSA) is a striking macromolecule often used i ...
Ion exchange chromatography
... Chromatography is separation method based on different retention of analytes on the stationary phase. Different chemical of physical phenomena can be used to retain an analyte. Depending on the phenomenon responsible for separation several types of chromatography are considered. Types of chromatogr ...
... Chromatography is separation method based on different retention of analytes on the stationary phase. Different chemical of physical phenomena can be used to retain an analyte. Depending on the phenomenon responsible for separation several types of chromatography are considered. Types of chromatogr ...
structbio_lecture_BCH339N_2016
... RiboA = 124 residues = 123 peptide bonds 2 torsion angles per peptide bond (phi and psi) = 246 degrees of freedom Assume 3 stable conformations per torsion angle = 3^(246) = 10^118 possible states Assume each state takes a picosecond to sample. = 10^20 years to test all states > 13.8 x 10^9 age of u ...
... RiboA = 124 residues = 123 peptide bonds 2 torsion angles per peptide bond (phi and psi) = 246 degrees of freedom Assume 3 stable conformations per torsion angle = 3^(246) = 10^118 possible states Assume each state takes a picosecond to sample. = 10^20 years to test all states > 13.8 x 10^9 age of u ...
Protein synthesis
... polypeptide chains Many polypeptide chains are covalently modified, either while they are still attached to the ribosome (cotranslational) or after their synthesis has been completed (posttranslational). These modifications may include removal of part of the translated sequence, or the covalent ...
... polypeptide chains Many polypeptide chains are covalently modified, either while they are still attached to the ribosome (cotranslational) or after their synthesis has been completed (posttranslational). These modifications may include removal of part of the translated sequence, or the covalent ...
EXPRESSION OF EQUIVALENT CLONOTYPES IN BALB/c
... paper are those detected within these limitations and must be regarded as the major sequences in the response while not excluding the possibility of other structures at undetectable levels. For experiments involving induced antibodies, such as these, TLC is used in our laboratory only qualitatively ...
... paper are those detected within these limitations and must be regarded as the major sequences in the response while not excluding the possibility of other structures at undetectable levels. For experiments involving induced antibodies, such as these, TLC is used in our laboratory only qualitatively ...
Lecture #2 – Review of Protein Chemistry, Enzyme Specificity
... natural choice as a biocatalyst. However, it would not have been surprising if the enzyme was not active against this compound either. Structural protein chemistry has made significant advances towards determining the nature of binding pockets, and software programs do exist to model substrate bindi ...
... natural choice as a biocatalyst. However, it would not have been surprising if the enzyme was not active against this compound either. Structural protein chemistry has made significant advances towards determining the nature of binding pockets, and software programs do exist to model substrate bindi ...
Scientific abstract
... Figure 1: Major players within the ethylene response pathway. Their activity has been given in both presence and absence of ethylene with the green boxes indicating an active status of the protein and the red boxes indicating an inactive status. ...
... Figure 1: Major players within the ethylene response pathway. Their activity has been given in both presence and absence of ethylene with the green boxes indicating an active status of the protein and the red boxes indicating an inactive status. ...
pdf-version
... or as annotated by UniProt or as predicted by Phobius. "transmembrane regions" — set the transmembrane regions by adding an arbitrary number of regions, see also Definition of the regions. You can interactively select from a pre-selected set of annotated regions by clicking + in the textfield. " ...
... or as annotated by UniProt or as predicted by Phobius. "transmembrane regions" — set the transmembrane regions by adding an arbitrary number of regions, see also Definition of the regions. You can interactively select from a pre-selected set of annotated regions by clicking + in the textfield. " ...
Chapter 2 - SCHOOLinSITES
... – Substrate binds to enzyme at active site – Enzymes act on substrates to reduce energy needed to make product – Substrate is changed – Enzyme separates from products and can form an association with another substrate – Enzyme, as a catalyst is not used up in the reaction – Increases reaction rate ...
... – Substrate binds to enzyme at active site – Enzymes act on substrates to reduce energy needed to make product – Substrate is changed – Enzyme separates from products and can form an association with another substrate – Enzyme, as a catalyst is not used up in the reaction – Increases reaction rate ...
Proteins include a diversity of structures
... Alterations in pH, salt concentration, temperature, or other environmental factors can cause a protein to unravel This loss of a protein’s native structure is called denaturation A denatured protein is biologically inactive ...
... Alterations in pH, salt concentration, temperature, or other environmental factors can cause a protein to unravel This loss of a protein’s native structure is called denaturation A denatured protein is biologically inactive ...
BIOLOGICAL MACROMOLECULES
... The advanced course on Biological Macromolecules is an introduction to various aspects of structure and function of proteins, nucleic acids, and their complexes. The topics addressed are a selection of modern biophysical methods applied to current questions in macromolecular biochemistry. In particu ...
... The advanced course on Biological Macromolecules is an introduction to various aspects of structure and function of proteins, nucleic acids, and their complexes. The topics addressed are a selection of modern biophysical methods applied to current questions in macromolecular biochemistry. In particu ...
V6-SecondaryStructur.. - Chair of Computational Biology
... (2) All methods optimise some parameters. Methods perform much better on proteins for which they were developed than on new proteins. ...
... (2) All methods optimise some parameters. Methods perform much better on proteins for which they were developed than on new proteins. ...
Biochemistry Lit Exam Concepts Soluble/Membrane protein function
... those from primary metabolism (e.g. glycolysis, citric acid cycle, fatty acid biosynthesis, etc.). be able to adapt the chemical logic from a primary metabolic pathway to that of a secondary metabolic pathway. DNA technology: Understand underpinnings of gene amplification by PCR and more advanced qu ...
... those from primary metabolism (e.g. glycolysis, citric acid cycle, fatty acid biosynthesis, etc.). be able to adapt the chemical logic from a primary metabolic pathway to that of a secondary metabolic pathway. DNA technology: Understand underpinnings of gene amplification by PCR and more advanced qu ...
Food - cbbiology
... Contain carbon, hydrogen, oxygen and nitrogen. Sometimes also contain small amounts of sulphur & phosphorus. Proteins are made up of amino acids. There are 20 different amino acids. Amino acids are joined together by peptide bonds. A peptide is made up of a small number of amino acids (less than 20) ...
... Contain carbon, hydrogen, oxygen and nitrogen. Sometimes also contain small amounts of sulphur & phosphorus. Proteins are made up of amino acids. There are 20 different amino acids. Amino acids are joined together by peptide bonds. A peptide is made up of a small number of amino acids (less than 20) ...
Review Questions
... b. ATP from the light reactions of photosynthesis. c. ATP from fermentation. d. ATP from glycolysis and cellular respiration. _____18. Which of the statements below about the citric acid cycle is false? a. The cycle releases less energy than glycolysis. b. CO2 is released during operation of the cyc ...
... b. ATP from the light reactions of photosynthesis. c. ATP from fermentation. d. ATP from glycolysis and cellular respiration. _____18. Which of the statements below about the citric acid cycle is false? a. The cycle releases less energy than glycolysis. b. CO2 is released during operation of the cyc ...
ImpactVector - 艾特克生物科技股份有限公司
... hybrid, which is notorious for low expression levels, at 0.3% in potato and the monocot garlic. These results illustrate that expression levels are always strongly gene and plant species dependent. To obtain expression levels in the range of 10% or more it may be necessary to optimize the genes firs ...
... hybrid, which is notorious for low expression levels, at 0.3% in potato and the monocot garlic. These results illustrate that expression levels are always strongly gene and plant species dependent. To obtain expression levels in the range of 10% or more it may be necessary to optimize the genes firs ...
Anti-MC5 Receptor antibody - Extracellular domain ab188932
... Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We invest ...
... Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We invest ...
here
... differentiation of ovarian granulosa cells in numerous mammalian species. EGF also stimulates the resumption of meiosis in oocytes that is necessary for complete maturation of the oocytes in preparation for fertilization. Inappropriately high levels of EGF or stimulation of EGF receptors is also com ...
... differentiation of ovarian granulosa cells in numerous mammalian species. EGF also stimulates the resumption of meiosis in oocytes that is necessary for complete maturation of the oocytes in preparation for fertilization. Inappropriately high levels of EGF or stimulation of EGF receptors is also com ...
Carbohydrates
... 2. Cellulose = (all β glucose – every other glucose is upside down; forms straight chain. - Due to structure, chains can stack on top of one another = Strong! - Major component cell walls in plants and some bacteria. Cell Identification w/Protein ….more later ...
... 2. Cellulose = (all β glucose – every other glucose is upside down; forms straight chain. - Due to structure, chains can stack on top of one another = Strong! - Major component cell walls in plants and some bacteria. Cell Identification w/Protein ….more later ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.