environmental life cycle assessment of alternative protein sources
... Very few LCA studies have been conducted on alternative proteins, like marine, insect or single cell protein Thus, clearly a need for more LCA studies in order to make fair comparisons – and improve and optimise the production Preliminary results from the few studies: For wet processes: the drying p ...
... Very few LCA studies have been conducted on alternative proteins, like marine, insect or single cell protein Thus, clearly a need for more LCA studies in order to make fair comparisons – and improve and optimise the production Preliminary results from the few studies: For wet processes: the drying p ...
The YidC/Oxa1/Alb3 protein family Saller, Manfred J.
... to deviate from the general consensus. For example, the core of Synechocystis sp. PCC6803 (Slr1471) is predicted to contain a large periplasmatic domain between core TMS2 and TMS3 that is conserved within cyanobacteria and is essential for activity. This region possibly assists in substrate protein ...
... to deviate from the general consensus. For example, the core of Synechocystis sp. PCC6803 (Slr1471) is predicted to contain a large periplasmatic domain between core TMS2 and TMS3 that is conserved within cyanobacteria and is essential for activity. This region possibly assists in substrate protein ...
uptake of nutrients-2014
... sugars (arabinose, maltose, galactose, ribose) and amino acids (glutamate, histidine, leucine) by this mechanism. Substances entering gram-negative bacteria must pass through the outer membrane before ABC transporters and other active transport systems can take action. There are several ways in whic ...
... sugars (arabinose, maltose, galactose, ribose) and amino acids (glutamate, histidine, leucine) by this mechanism. Substances entering gram-negative bacteria must pass through the outer membrane before ABC transporters and other active transport systems can take action. There are several ways in whic ...
Chemistry PPT
... throughout human history. • However, sugars are not the only substances perceived as sweet; there are other chemicals that can trigger the same sensation. • We perceive sweetness when molecules of a substance attach to the “sweet” taste receptors on our tongue, triggering a message to the brain. • M ...
... throughout human history. • However, sugars are not the only substances perceived as sweet; there are other chemicals that can trigger the same sensation. • We perceive sweetness when molecules of a substance attach to the “sweet” taste receptors on our tongue, triggering a message to the brain. • M ...
Medical Biology
... which egg is transported all so trachea is lined with cilia which move the slimy mucous that traps dust particles and prevents getting into lung 12- Microfilaments and microtubules The cytoplasm of all eukaryotic cells is crossed by a network of protein fibers that supports the shape of the shape of ...
... which egg is transported all so trachea is lined with cilia which move the slimy mucous that traps dust particles and prevents getting into lung 12- Microfilaments and microtubules The cytoplasm of all eukaryotic cells is crossed by a network of protein fibers that supports the shape of the shape of ...
Polymer Molecules
... All proteins contain the elements C,O,H, N. They are condensation polymers, made by amino acids linking together. An amine group of one molecule links to the carboxyl group of another molecule to form an amide or peptide bond. The body cannot make every type of amino acids that it needs. So our diet ...
... All proteins contain the elements C,O,H, N. They are condensation polymers, made by amino acids linking together. An amine group of one molecule links to the carboxyl group of another molecule to form an amide or peptide bond. The body cannot make every type of amino acids that it needs. So our diet ...
1.2a Chemistry of Life
... throughout human history. • However, sugars are not the only substances perceived as sweet; there are other chemicals that can trigger the same sensation. • We perceive sweetness when molecules of a substance attach to the “sweet” taste receptors on our tongue, triggering a message to the brain. • M ...
... throughout human history. • However, sugars are not the only substances perceived as sweet; there are other chemicals that can trigger the same sensation. • We perceive sweetness when molecules of a substance attach to the “sweet” taste receptors on our tongue, triggering a message to the brain. • M ...
HL-SAN for DNA removal in protein purification - A4
... The amount of HL-SAN needed for DNA removal from a cell extract or lysate depends on several factors; expression strain, target protein, lysis buffer composition, NaCl concentration, etc. The following is therefore considered as guidelines: Add 1000 U HL-SAN per ml sample with 0.3–0.75 M NaCl and in ...
... The amount of HL-SAN needed for DNA removal from a cell extract or lysate depends on several factors; expression strain, target protein, lysis buffer composition, NaCl concentration, etc. The following is therefore considered as guidelines: Add 1000 U HL-SAN per ml sample with 0.3–0.75 M NaCl and in ...
bioCHEMISTRY 480 Molecular Biochemistry-‐
... In addition in the QP500-‐600 Section of JFK Library [lower right hand back corner] there is a wide variety of biochemistry books. Some of these are general undergraduate textbooks whereas many others ...
... In addition in the QP500-‐600 Section of JFK Library [lower right hand back corner] there is a wide variety of biochemistry books. Some of these are general undergraduate textbooks whereas many others ...
... c) (4 pts) You prepare a scientific paper on these experiments, in which you claim that the energy of a SH hydrogen bond is 10 kJ/mol weaker than the energy of an OH hydrogen bond. This claim is rejected by the reviewers of your paper! Although your measurements are correct, what mistake have you ma ...
Supplementary Methods
... incubated with 3ul anti-Flag Ab or 1ul anti-Myc Ab for 3 hours. 20ul Protein G plus Agarose beads were then added and incubated for 2 hours. The beads with protein complex were washed with 1x lysis buffer 4 times and loaded onto an SDS-PAGE gel after being heated. The proteins were then transferred ...
... incubated with 3ul anti-Flag Ab or 1ul anti-Myc Ab for 3 hours. 20ul Protein G plus Agarose beads were then added and incubated for 2 hours. The beads with protein complex were washed with 1x lysis buffer 4 times and loaded onto an SDS-PAGE gel after being heated. The proteins were then transferred ...
MCB Lecture 6 – Lysosomes
... o The M6P tag is not added to the enzymes that should be going to the lysosome. Because they do not have this tag, they are secreted extracellularly. The lysosome then has no enzymes to break down ANYTHING, leading to an accumulation of undigested substrates. What are some symptoms of I-Cell Disea ...
... o The M6P tag is not added to the enzymes that should be going to the lysosome. Because they do not have this tag, they are secreted extracellularly. The lysosome then has no enzymes to break down ANYTHING, leading to an accumulation of undigested substrates. What are some symptoms of I-Cell Disea ...
Document
... Glucose (C6H12O6) + 2 ADP + 2 Pi + 2 NAD+ (cytoplasm) + 8 NAD+ + 2 FAD + 2 GDP + 2Pi + 2 H2O (mitochondria) ...
... Glucose (C6H12O6) + 2 ADP + 2 Pi + 2 NAD+ (cytoplasm) + 8 NAD+ + 2 FAD + 2 GDP + 2Pi + 2 H2O (mitochondria) ...
Jenae`s BLAST search 10-23-12
... This is what I have so far. I have no idea if I even did any of this correctly. I have no idea what the next step should be. I don’t know if this is helpful to any of you, but I wanted to play around with the BLAST search to see if I could even run it. Since this is possibly the chloroplast genome, ...
... This is what I have so far. I have no idea if I even did any of this correctly. I have no idea what the next step should be. I don’t know if this is helpful to any of you, but I wanted to play around with the BLAST search to see if I could even run it. Since this is possibly the chloroplast genome, ...
Biomolecular chemistry 4. From amino acids to proteins
... • A reverse turn is region of the polypeptide having a hydrogen bond from one main chain carbonyl oxygen to the main chain N-H group 3 residues along the chain (i.e. O(i) to N(i+3)). Helical regions are excluded from this definition and turns between beta-strands form a special class of turn known a ...
... • A reverse turn is region of the polypeptide having a hydrogen bond from one main chain carbonyl oxygen to the main chain N-H group 3 residues along the chain (i.e. O(i) to N(i+3)). Helical regions are excluded from this definition and turns between beta-strands form a special class of turn known a ...
File - Hoblitzell`s Science Spot
... b. the loss of amino acids by excretion through the kidneys when cells die. c. the use of amino acids that have been conserved from breakdown of old cells. d. the annual replacement of old cells with new protein that has entered the body in food. ...
... b. the loss of amino acids by excretion through the kidneys when cells die. c. the use of amino acids that have been conserved from breakdown of old cells. d. the annual replacement of old cells with new protein that has entered the body in food. ...
BITC1311 Intro to Biotechnology
... a. What sort of affinity ligand would be used in affinity chromatography of a fusion protein containing a glutathione-S-transferase tag? How would you remove the purified protein from such a column? b. How can a tag protein be removed from a fusion protein? c. What are lux genes, and how are they us ...
... a. What sort of affinity ligand would be used in affinity chromatography of a fusion protein containing a glutathione-S-transferase tag? How would you remove the purified protein from such a column? b. How can a tag protein be removed from a fusion protein? c. What are lux genes, and how are they us ...
Biotechnology Lab (Kallas)
... plasmid (pOSH37/GFP, which encodes an engineered “fusion” protein containing parts of the proteins thioredoxin, the jellyfish Green Fluorescent Protein, and an iron-sulfur protein), into a bacterial expression strain (E. coli AD494(DE3)) for “overproduction” of the “fusion” protein. We will then pur ...
... plasmid (pOSH37/GFP, which encodes an engineered “fusion” protein containing parts of the proteins thioredoxin, the jellyfish Green Fluorescent Protein, and an iron-sulfur protein), into a bacterial expression strain (E. coli AD494(DE3)) for “overproduction” of the “fusion” protein. We will then pur ...
Review Article
... vitro and translocated into yeast ER membranes where it is modified by core-glycosylation. Protein that is subsequently transported to the Golgi apparatus undergoes outer-chain glycosylation and can be identified by antibodies specific for outer-chain carbohydrate. When these reactions are performed ...
... vitro and translocated into yeast ER membranes where it is modified by core-glycosylation. Protein that is subsequently transported to the Golgi apparatus undergoes outer-chain glycosylation and can be identified by antibodies specific for outer-chain carbohydrate. When these reactions are performed ...
Biochem Review, Part I: Protein Structure and Function
... Ion Exchange Chromatography 1. Digest protein in strong acid 2. Load onto column of negatively charged (sulfonate coated) beads; AAs bind because positively charged 3. Wash column with solutions of increasing pH, collecting eluate in small fractions 4. For each fraction, determine the presence/abse ...
... Ion Exchange Chromatography 1. Digest protein in strong acid 2. Load onto column of negatively charged (sulfonate coated) beads; AAs bind because positively charged 3. Wash column with solutions of increasing pH, collecting eluate in small fractions 4. For each fraction, determine the presence/abse ...
WHAT IS?Protein is an essential nutritional product for the growth
... acids. It is the fats that supply the body with vitamin A and D. When eating low fat foods and lean meats, use caution as this can create a deficiency in vitamin A, D and B12. A diet low in animal proteins will also be low in important minerals such as zinc, iron and calcium. Zinc deficiency can lea ...
... acids. It is the fats that supply the body with vitamin A and D. When eating low fat foods and lean meats, use caution as this can create a deficiency in vitamin A, D and B12. A diet low in animal proteins will also be low in important minerals such as zinc, iron and calcium. Zinc deficiency can lea ...
Some statistical musings
... But … we have some high probability that all the “important” features are selected (along with others which we will screen out later). ...
... But … we have some high probability that all the “important” features are selected (along with others which we will screen out later). ...
Proteins in Serum and Urine - Ústav lékařské biochemie a
... Simply stated, the acute phase reaction is triggered by conditions that include destruction of cells, reversible cell damage followed by reparation, or metabolic activation of cells participating in the immune response. During the acute phase reaction the involved cells produce many mediators and si ...
... Simply stated, the acute phase reaction is triggered by conditions that include destruction of cells, reversible cell damage followed by reparation, or metabolic activation of cells participating in the immune response. During the acute phase reaction the involved cells produce many mediators and si ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.