A Survey of Recent Work on Evolutionary Approaches to the Protein
... replication of the AIDS virus depends on the HIV protease enzyme. If one can nd a small molecule that will permanently bind to the active site in the HIV protease, the normal function of that enzyme can be prevented. Autodock [17] is an existing docking software package, which is designed to invest ...
... replication of the AIDS virus depends on the HIV protease enzyme. If one can nd a small molecule that will permanently bind to the active site in the HIV protease, the normal function of that enzyme can be prevented. Autodock [17] is an existing docking software package, which is designed to invest ...
Biology 301 Exam 3 Name Spring 2008 1. Which of the following is
... C. ubiquinone D. all of the choices 2. Which of the following is not true about enzymes? A. Enzymes are catalysts that speed up reactions. B. Enzymes are proteins that can be denatured by changes in pH or temperature. C. Enzymes are highly specific for the substrates they react with and catalyze onl ...
... C. ubiquinone D. all of the choices 2. Which of the following is not true about enzymes? A. Enzymes are catalysts that speed up reactions. B. Enzymes are proteins that can be denatured by changes in pH or temperature. C. Enzymes are highly specific for the substrates they react with and catalyze onl ...
2-D Quant Kit - GE Healthcare Life Sciences
... The 2-D Quant Kit circumvents these limitations and can be used to accurately quantify protein samples prepared for 2-D electrophoresis as well as any sample that contains substances that otherwise interfere with protein quantification. The procedure uses a combination of a unique precipitant and co ...
... The 2-D Quant Kit circumvents these limitations and can be used to accurately quantify protein samples prepared for 2-D electrophoresis as well as any sample that contains substances that otherwise interfere with protein quantification. The procedure uses a combination of a unique precipitant and co ...
Food biomolecules
... 8. Name the reagents used to test for a reducing sugar. 9. State one way in which carbohydrates differ from fats. 10. Name the reagents used to test for a protein. 11. Cellulose is a polysaccharide. What is it formed from? 12. Name an important group of biomolecules that plants make from nitrate ...
... 8. Name the reagents used to test for a reducing sugar. 9. State one way in which carbohydrates differ from fats. 10. Name the reagents used to test for a protein. 11. Cellulose is a polysaccharide. What is it formed from? 12. Name an important group of biomolecules that plants make from nitrate ...
Peroxisomes - University of California San Diego
... A 100 –50 kDa ATPase (AAA family) in yeasts and humans. Interacts with Pex6 and other peroxins. Defects in Pex1 are the most common cause of the PBDs (CGI). A ,40 kDa integral PMP with a carboxy-terminal, cytoplasmically exposed, zinc-binding RING domain. Has been identified in yeasts and humans, in ...
... A 100 –50 kDa ATPase (AAA family) in yeasts and humans. Interacts with Pex6 and other peroxins. Defects in Pex1 are the most common cause of the PBDs (CGI). A ,40 kDa integral PMP with a carboxy-terminal, cytoplasmically exposed, zinc-binding RING domain. Has been identified in yeasts and humans, in ...
Heterologous protein production of a His-tagged peroxidase
... Unfortunately, since every protein is different, in many cases production and purification protocols and strategies must be developed for each individual protein. In E. coli the fusion of proteins to oligo-histidine tags followed by affinity chromatography is a very common protein purification strat ...
... Unfortunately, since every protein is different, in many cases production and purification protocols and strategies must be developed for each individual protein. In E. coli the fusion of proteins to oligo-histidine tags followed by affinity chromatography is a very common protein purification strat ...
SFE1, a Constituent of the Fertilization Envelope
... to SDS-PAGE [19] followed by immunoblot analysis essentially as described [20]. Protein samples were pelleted, resuspended in SDS-PAGE sample buffer containing 10 mM dithiothreitol and a protease inhibitor cocktail (consisting of a final concentration per ml of aprotinin, 1 TIU; benzamidine, 10 mg; ...
... to SDS-PAGE [19] followed by immunoblot analysis essentially as described [20]. Protein samples were pelleted, resuspended in SDS-PAGE sample buffer containing 10 mM dithiothreitol and a protease inhibitor cocktail (consisting of a final concentration per ml of aprotinin, 1 TIU; benzamidine, 10 mg; ...
Hendra Virus
... • Hendra virus and Nipah virus are very closely related, and their V proteins inhibit interferon signaling in very similar ways. • While human infections of these diseases remain fairly low, a good understanding of them is helpful, since both are zoonotic diseases (passed from animals to humans). • ...
... • Hendra virus and Nipah virus are very closely related, and their V proteins inhibit interferon signaling in very similar ways. • While human infections of these diseases remain fairly low, a good understanding of them is helpful, since both are zoonotic diseases (passed from animals to humans). • ...
Database Searching and Pairwise Alignment
... • Choose one sequence to be searched against the other • Query sequence (q) and target sequence (t) • Divide the query sequence into small subsequences, called words • For each word of q, look along t to find other words in t which are similar • Matching words "anchors" build up a better alignment b ...
... • Choose one sequence to be searched against the other • Query sequence (q) and target sequence (t) • Divide the query sequence into small subsequences, called words • For each word of q, look along t to find other words in t which are similar • Matching words "anchors" build up a better alignment b ...
New roles for structure in biology and drug discovery
... screening, but these must be adapted to meet the quality and quantity demands of a structural genomics approach. Recent advances have produced an increase in the speed of macromolecular structure determination8–10. For X-ray crystallography, developments like seleno-methionine derivatives, cryo-free ...
... screening, but these must be adapted to meet the quality and quantity demands of a structural genomics approach. Recent advances have produced an increase in the speed of macromolecular structure determination8–10. For X-ray crystallography, developments like seleno-methionine derivatives, cryo-free ...
ppt file
... It has been shown for many proteins that it is possible to engineer higher stability by introducing mutations. In many cases, this does not appear to impair activity in in vivo and/or in vitro assays. Moreover, comparable proteins from thermophilic organisms have higher stability than those from mes ...
... It has been shown for many proteins that it is possible to engineer higher stability by introducing mutations. In many cases, this does not appear to impair activity in in vivo and/or in vitro assays. Moreover, comparable proteins from thermophilic organisms have higher stability than those from mes ...
Amino Acids - Building Blocks of Proteins
... chains don’t like water. Hydrophobic side chains are also referred to as non-polar side chains. Now can you guess what hydrophilic means? Philic means likes or attracted to, so hydrophilic side chains like water. Hydrophilic side chains are also referred to as polar side chains. Acidic (Negatively C ...
... chains don’t like water. Hydrophobic side chains are also referred to as non-polar side chains. Now can you guess what hydrophilic means? Philic means likes or attracted to, so hydrophilic side chains like water. Hydrophilic side chains are also referred to as polar side chains. Acidic (Negatively C ...
TDH - an Enzyme Involved in Metabolising Threonine to Glycine
... The data were processed using programs in the CCP4 suite, however the predicted spacegroup was ambiguous and took time to determine correctly. P4 was found to generate the best predictions in MOSFLM and the data was subsequently processed and scaled, giving an Rmerge of 0.154. It was later processed ...
... The data were processed using programs in the CCP4 suite, however the predicted spacegroup was ambiguous and took time to determine correctly. P4 was found to generate the best predictions in MOSFLM and the data was subsequently processed and scaled, giving an Rmerge of 0.154. It was later processed ...
Hand Outs B 1 - University of Wisconsin–Madison
... Amino Acid=a small unit that makes up protein Phenylalanine=one of 20 amino acids. Called “phe” for short. Tyrosine=another amino acid. In someone who does not have PKU, phe gets changed into tyrosine. PKU=Phenylketonuria=a genetic disorder in which the body cannot change phe in the usual way. Enzym ...
... Amino Acid=a small unit that makes up protein Phenylalanine=one of 20 amino acids. Called “phe” for short. Tyrosine=another amino acid. In someone who does not have PKU, phe gets changed into tyrosine. PKU=Phenylketonuria=a genetic disorder in which the body cannot change phe in the usual way. Enzym ...
... treatments and seven replicates in four periods of 28 days/each was used. The treatments were: Control - Formulated according to the nutritional requirements proposed in the strain, containing 16.92% crude protein, 0.750% digestible lysine. Treatments 1 to 5, with crude protein levels of 14% and 0.6 ...
Chem*3560 Lecture 31: Ion selective channels
... Ion selective channels allow specific ions to pass through a membrane bilayer at a very high rate. They serve a very different purpose than simple transporters or pumps, so some definition of terms is appropriate (Lehninger p.424-5). A transporter (uniporter, symporter or antiporter) is a protein wi ...
... Ion selective channels allow specific ions to pass through a membrane bilayer at a very high rate. They serve a very different purpose than simple transporters or pumps, so some definition of terms is appropriate (Lehninger p.424-5). A transporter (uniporter, symporter or antiporter) is a protein wi ...
Genomic organization of infectious salmon anaemia virus
... that does not affect influenza A or C virus haemagglutination, suggesting that the receptors are different for the viruses. Recent evidence shows that ISAV is similar to orthomyxoviruses in that it binds to sialic acid residues on host cell surfaces and undergoes fusion with the cell in acidic endos ...
... that does not affect influenza A or C virus haemagglutination, suggesting that the receptors are different for the viruses. Recent evidence shows that ISAV is similar to orthomyxoviruses in that it binds to sialic acid residues on host cell surfaces and undergoes fusion with the cell in acidic endos ...
Question Answers 2(Download)
... others to cross it in certain ways, at certain times. If the concentration of a substance in one region differs from that in an adjoining region, it is called a (2) ________. A (3) ________ is a difference between the number of molecules or ions of a given substance in adjoining regions. (4) _______ ...
... others to cross it in certain ways, at certain times. If the concentration of a substance in one region differs from that in an adjoining region, it is called a (2) ________. A (3) ________ is a difference between the number of molecules or ions of a given substance in adjoining regions. (4) _______ ...
Chapter 5: What are the major types of organic molecules?
... tertiary structure (3) of a protein is the overall folded shape of a single polypeptide chain, determined by secondary structure combined with interactions between R groups (NOTE: book defines this in a confusing way, use my way) ...
... tertiary structure (3) of a protein is the overall folded shape of a single polypeptide chain, determined by secondary structure combined with interactions between R groups (NOTE: book defines this in a confusing way, use my way) ...
Constitutive heat shock protein 70 (HSC70) expression in rainbow
... The tissue protein concentrations were determined by the BCA method with bovine serum albumin (BSA) as the standard. The samples were analyzed on 8% polyacrylamide gels using the discontinuous buffer system of Laemmli (1970). The gels were either stained with Bio-Safe Coomassie stain or were transfe ...
... The tissue protein concentrations were determined by the BCA method with bovine serum albumin (BSA) as the standard. The samples were analyzed on 8% polyacrylamide gels using the discontinuous buffer system of Laemmli (1970). The gels were either stained with Bio-Safe Coomassie stain or were transfe ...
Poster
... he A-beta protein and transthyretin are two proteins of interest to scientists trying to understand how Alzheimer’s disease develops. The disease results from the accumulation of a specific fragment of the amyloid precursor protein. When this small piece of protein is cut from the amyloid precursor ...
... he A-beta protein and transthyretin are two proteins of interest to scientists trying to understand how Alzheimer’s disease develops. The disease results from the accumulation of a specific fragment of the amyloid precursor protein. When this small piece of protein is cut from the amyloid precursor ...
Fuel Metabolism
... that is found in large masses in the interscapular region, the perirenal area, and surrounds the aorta and heart of the hibernator. BAT proliferation and differentiation is responsive to multiple signals including insulin and insulin-dependent growth factor (IGF-I) that are particularly involved i ...
... that is found in large masses in the interscapular region, the perirenal area, and surrounds the aorta and heart of the hibernator. BAT proliferation and differentiation is responsive to multiple signals including insulin and insulin-dependent growth factor (IGF-I) that are particularly involved i ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.