A protein domain interaction interface database: InterPare | BMC
... whole protein configuration, can be viewed with three different molecular viewers on the results page. They are the Chime [24], Jmol [25], and InterFacer [26]. InterPare also provides the atomic coordinate files for protein surface, interior, and interface for further analysis. ...
... whole protein configuration, can be viewed with three different molecular viewers on the results page. They are the Chime [24], Jmol [25], and InterFacer [26]. InterPare also provides the atomic coordinate files for protein surface, interior, and interface for further analysis. ...
Branching morphogenesis of the Drosophila tracheal system. Annual Review of Cell Developmental Biology 19, 623-647. pdf
... tracheal placodes fail to invaginate (Boube et al. 2000). Ubiquitous expression of trachealess can induce ectopic tracheal marker expression and invagination, although this ability is spatially restricted. This restriction, together with the finding that some tracheal genes (vvl, kni, knrl, tkv) are ...
... tracheal placodes fail to invaginate (Boube et al. 2000). Ubiquitous expression of trachealess can induce ectopic tracheal marker expression and invagination, although this ability is spatially restricted. This restriction, together with the finding that some tracheal genes (vvl, kni, knrl, tkv) are ...
Glucose-6-phosphate Metabolic Preferential Destinations
... G6P content consumption (Figure 2A). One previous study, using co-culture systems, suggested regional differences associated with the physiological support provided by the epithelial cells from oviduct [1]. Glycolysis is the metabolic pathway that converts glucose into pyruvate . The first step in g ...
... G6P content consumption (Figure 2A). One previous study, using co-culture systems, suggested regional differences associated with the physiological support provided by the epithelial cells from oviduct [1]. Glycolysis is the metabolic pathway that converts glucose into pyruvate . The first step in g ...
Patterning the Xenopus blastula - Development
... mRNAs are present in the oocyte and early embryo and have dorsalizing activity when injected into embryos (Xwnt 11: Ku and Melton, 1993; Xwnt-8b: Cui et al., 1995). But the roles of the endogenous molecules have yet to be defined. Recent studies have indicated that homologs of the Drosophila frizzle ...
... mRNAs are present in the oocyte and early embryo and have dorsalizing activity when injected into embryos (Xwnt 11: Ku and Melton, 1993; Xwnt-8b: Cui et al., 1995). But the roles of the endogenous molecules have yet to be defined. Recent studies have indicated that homologs of the Drosophila frizzle ...
Acetylcholine Receptor-associated 43K Protein Contains Covalently
... structural similarities. The amino acid sequence of Torpedo 43K protein was determined by cDNA cloning (20) and direct protein sequencing of purified 43K protein (16). Consistent with the known physical properties of Torpedo 43K protein, the sequence was found to be very rich in cysteine and lacking ...
... structural similarities. The amino acid sequence of Torpedo 43K protein was determined by cDNA cloning (20) and direct protein sequencing of purified 43K protein (16). Consistent with the known physical properties of Torpedo 43K protein, the sequence was found to be very rich in cysteine and lacking ...
Two highly related regulatory subunits of PP2A exert opposite
... in situ hybridisation probes corresponding to nucleotides 1988-2176 and 9194 of the full-length mRNA, respectively, were generated by PCR from Xenopus cDNA libraries and cloned into pCR2.1 (Invitrogen). Xbra and gsc probes (Howell et al., 2002), pCS2-GFP, pFTX4K-EGFPSmad2 and the plasmid expressing ...
... in situ hybridisation probes corresponding to nucleotides 1988-2176 and 9194 of the full-length mRNA, respectively, were generated by PCR from Xenopus cDNA libraries and cloned into pCR2.1 (Invitrogen). Xbra and gsc probes (Howell et al., 2002), pCS2-GFP, pFTX4K-EGFPSmad2 and the plasmid expressing ...
Distinct and collaborative roles of Drosophila EXT family proteins in
... demonstrated. HS GAG is strikingly reduced, but not completely eliminated, in the ttv null embryo (The et al., 1999). Biochemical analysis further showed that HS GAG is markedly reduced in ttv mutant larvae (Toyoda et al., 2000). Together, these studies have demonstrated that Ttv is required for Hh ...
... demonstrated. HS GAG is strikingly reduced, but not completely eliminated, in the ttv null embryo (The et al., 1999). Biochemical analysis further showed that HS GAG is markedly reduced in ttv mutant larvae (Toyoda et al., 2000). Together, these studies have demonstrated that Ttv is required for Hh ...
Exploration of the Dynamic Properties of Protein Complexes
... shows that protein A interacts with B, B interacts with C and A does not interact with C (Figure 3A). One possible conformation is shown in Figure 3B with the constraints that are required to be satisfied (Figure 3C,D). A more detailed example is illustrated in Figure S1. A non-interacting pair prov ...
... shows that protein A interacts with B, B interacts with C and A does not interact with C (Figure 3A). One possible conformation is shown in Figure 3B with the constraints that are required to be satisfied (Figure 3C,D). A more detailed example is illustrated in Figure S1. A non-interacting pair prov ...
The development and evolution of crossveins in insect wings
... wing and has been reviewed by Conley et al. (2000). Manipulations of many different components of this signalling pathway result in the loss of crossveins. Homozygotes of hypomorphic alleles of the BMP-like ligand Glass Bottom Boat (Gbb) show a complete loss of both the anterior and posterior crossv ...
... wing and has been reviewed by Conley et al. (2000). Manipulations of many different components of this signalling pathway result in the loss of crossveins. Homozygotes of hypomorphic alleles of the BMP-like ligand Glass Bottom Boat (Gbb) show a complete loss of both the anterior and posterior crossv ...
Summary and Conclusion Facultyof Pharmacy Department of
... many complications threatening life. Diabetic nephropathy (DN) remains the most common cause for end stage renal disease. Adenosine is an endogenous purine nucleoside released from various tissues and organs. Adenosine controls the supply and demand of energy and modulates a variety of physiological ...
... many complications threatening life. Diabetic nephropathy (DN) remains the most common cause for end stage renal disease. Adenosine is an endogenous purine nucleoside released from various tissues and organs. Adenosine controls the supply and demand of energy and modulates a variety of physiological ...
YEO_RNA_2006
... REST sites are highly-conserved across evolution Superiorcervical ganglion neuralspecific 10 Synaptosomalassociated protein 25 ...
... REST sites are highly-conserved across evolution Superiorcervical ganglion neuralspecific 10 Synaptosomalassociated protein 25 ...
Viral Strategies in Modulation of NF
... regulatory subunit IKKγ68. These kinases phosphorylate specific serine residues at the N-terminal region of IκB molecules, the phosphorylated IκBs are then ubiquitinated, which is a signal for their degradation by the 26S proteasome33. NF-κB is then released and translocates to the nucleus, where it ...
... regulatory subunit IKKγ68. These kinases phosphorylate specific serine residues at the N-terminal region of IκB molecules, the phosphorylated IκBs are then ubiquitinated, which is a signal for their degradation by the 26S proteasome33. NF-κB is then released and translocates to the nucleus, where it ...
Dynamin and the Actin Cytoskeleton Cooperatively Regulate
... the Jnet program (Cuff and Barton, 2000) suggested a predominantly a-helical structure for the entire FCHCC region. Further analysis aligned this entire amino acid sequence with the BAR domain and predicted, as in the case of the BAR domain, three distinct helices (Figure 1A). Thus, the FCH domain, ...
... the Jnet program (Cuff and Barton, 2000) suggested a predominantly a-helical structure for the entire FCHCC region. Further analysis aligned this entire amino acid sequence with the BAR domain and predicted, as in the case of the BAR domain, three distinct helices (Figure 1A). Thus, the FCH domain, ...
PDF
... 2008; Olmedo-Verd et al., 2011). Membrane anchoring was particularly well demonstrated for the Valyl-tRNA synthetase (ValRS) of Anabaena sp. PCC 7120 (hereafter Anabaena), which harbors an idiosyncratic domain, termed CAAD, with a putative C-terminal coiled-coil and two transmembrane helices, that m ...
... 2008; Olmedo-Verd et al., 2011). Membrane anchoring was particularly well demonstrated for the Valyl-tRNA synthetase (ValRS) of Anabaena sp. PCC 7120 (hereafter Anabaena), which harbors an idiosyncratic domain, termed CAAD, with a putative C-terminal coiled-coil and two transmembrane helices, that m ...
Disruption of early proximodistal patterning and AVE
... of an earlier AP induction signal. -Catenin mutant embryos (Haegel et al., 1995; Huelsken et al., 2000) also fail to form mesendoderm and they completely lack expression of mesoderm markers such as T and goosecoid (Gsc). However, they also show defects in the AVE not shown by Wnt3 mutants. They fai ...
... of an earlier AP induction signal. -Catenin mutant embryos (Haegel et al., 1995; Huelsken et al., 2000) also fail to form mesendoderm and they completely lack expression of mesoderm markers such as T and goosecoid (Gsc). However, they also show defects in the AVE not shown by Wnt3 mutants. They fai ...
Over-expression screen in Drosophila identifies neuronal
... been performed to identify genes that have altered expression in the rodent brain reward pathway (nucleus accumbens) following transgenic expression of delta-FosB (an inhibitory isoform of mammalian Fos) (MCCLUNG and NESTLER 2003). Persistent Fos isoform switching followed by prolonged presence of ...
... been performed to identify genes that have altered expression in the rodent brain reward pathway (nucleus accumbens) following transgenic expression of delta-FosB (an inhibitory isoform of mammalian Fos) (MCCLUNG and NESTLER 2003). Persistent Fos isoform switching followed by prolonged presence of ...
Arterial–Venous Specification During Development
... capillary plexus (intussusceptive angiogenesis). In later development and adult life, these 2 types of vessel formation processes often occur together and the distinction between them is frequently not so clear. In addition to the earliest extraembryonic and lateral mesoderm, angioblasts are also th ...
... capillary plexus (intussusceptive angiogenesis). In later development and adult life, these 2 types of vessel formation processes often occur together and the distinction between them is frequently not so clear. In addition to the earliest extraembryonic and lateral mesoderm, angioblasts are also th ...
Analysis of p75NTR-dependent apoptotic
... Research Compliance Certificate: McGill University Animal Use Protocol ...
... Research Compliance Certificate: McGill University Animal Use Protocol ...
Chaperone-assisted protein folding: the path to discovery from a
... a-helices and b-sheets, such as the TIM barrel, ...
... a-helices and b-sheets, such as the TIM barrel, ...
Drosophila ventral furrow morphogenesis: a
... during ventral furrow formation, the proteomes of ventral cells and lateral cells were compared. Lateral cells were chosen over dorsal cells because they maintain their columnar shape during gastrulation; dorsal cells adopt a squamous epithelial shape. To obtain relatively pure populations of ventra ...
... during ventral furrow formation, the proteomes of ventral cells and lateral cells were compared. Lateral cells were chosen over dorsal cells because they maintain their columnar shape during gastrulation; dorsal cells adopt a squamous epithelial shape. To obtain relatively pure populations of ventra ...
11 Enzymes - School of Chemistry and Biochemistry
... Wikipedia: BPTI is one of the most thoroughly studied proteins by structural biology methods, experimental and computational dynamics, mutagenesis, and folding experiments. It was one of the earliest protein crystal structures solved, in 1970 in the laboratory of Robert Huber, and was the first prot ...
... Wikipedia: BPTI is one of the most thoroughly studied proteins by structural biology methods, experimental and computational dynamics, mutagenesis, and folding experiments. It was one of the earliest protein crystal structures solved, in 1970 in the laboratory of Robert Huber, and was the first prot ...
During Cytokinesis Light Chain, Tctex
... Tctex-1 (amino acids 1–113). The pPC86 plasmid encodes the Gal4 transcriptional activation domain (amino acids 767– 881) and 39 restriction sites for generation of “prey” fusion protein constructs. A cDNA library was prepared from 5 mg of poly(A)1-enriched RNA that had been isolated from the K46 B c ...
... Tctex-1 (amino acids 1–113). The pPC86 plasmid encodes the Gal4 transcriptional activation domain (amino acids 767– 881) and 39 restriction sites for generation of “prey” fusion protein constructs. A cDNA library was prepared from 5 mg of poly(A)1-enriched RNA that had been isolated from the K46 B c ...
Resistance Gene-Dependent Activation of a Calcium
... kinase (Song et al., 1995). In addition, in vivo phosphorylation experiments and studies with pharmacological inhibitors have demonstrated that protein kinases and phosphatases are crucial for activation of early defense responses (Yang et al., 1997; Scheel, 1998). Recent reports have noted the acti ...
... kinase (Song et al., 1995). In addition, in vivo phosphorylation experiments and studies with pharmacological inhibitors have demonstrated that protein kinases and phosphatases are crucial for activation of early defense responses (Yang et al., 1997; Scheel, 1998). Recent reports have noted the acti ...
Dubrulle and Pourquie, 2004 - Development
... a series of stereotypical events, from its specification in the caudal part of the embryo to its incorporation into a somite (Fig. 1A). The first step in paraxial mesoderm formation is a change in the cellular adhesion properties. Cells contained in the primitive streak and fated to become paraxial ...
... a series of stereotypical events, from its specification in the caudal part of the embryo to its incorporation into a somite (Fig. 1A). The first step in paraxial mesoderm formation is a change in the cellular adhesion properties. Cells contained in the primitive streak and fated to become paraxial ...
FoxF is essential for FGF-induced migration of heart
... the heart precursor cells in ascidian embryos. During subsequent stages of embryogenesis, they migrate into the trunk and fuse at the ventral midline in a fashion reminiscent of vertebrate heart precursor cells (Davidson and Levine, 2003). Previous studies illuminated some aspects of the genetic reg ...
... the heart precursor cells in ascidian embryos. During subsequent stages of embryogenesis, they migrate into the trunk and fuse at the ventral midline in a fashion reminiscent of vertebrate heart precursor cells (Davidson and Levine, 2003). Previous studies illuminated some aspects of the genetic reg ...
Hedgehog signaling pathway
The Hedgehog signaling pathway (or signalling pathway; see spelling differences) is a signaling pathway that transmits information to embryonic cells required for proper development. Different parts of the embryo have different concentrations of hedgehog signaling proteins. The pathway also has roles in the adult. Diseases associated with the malfunction of this pathway include basal cell carcinoma.The Hedgehog signaling pathway is one of the key regulators of animal development and is present in all bilaterians. The pathway takes its name from its polypeptide ligand, an intercellular signaling molecule called Hedgehog (Hh) found in fruit flies of the genus Drosophila. Hh is one of Drosophila's segment polarity gene products, involved in establishing the basis of the fly body plan. The molecule remains important during later stages of embryogenesis and metamorphosis.Mammals have three Hedgehog homologues, DHH, IHH, and SHH, of which Sonic (SHH) is the best studied. The pathway is equally important during vertebrate embryonic development. In knockout mice lacking components of the pathway, the brain, skeleton, musculature, gastrointestinal tract and lungs fail to develop correctly. Recent studies point to the role of Hedgehog signaling in regulating adult stem cells involved in maintenance and regeneration of adult tissues. The pathway has also been implicated in the development of some cancers. Drugs that specifically target Hedgehog signaling to fight this disease are being actively developed by a number of pharmaceutical companies.