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Manufacturing Muscle: Protein

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... in lysis buffer containing 1 X BugBuster Protein Extraction Reagent (Novagen EMD Chemicals, Inc. CA), Lysonase™ Bioprocessing Reagent (Novagen EMD Chemicals, Inc. CA), 1 X phosphatebuffered saline, and 10 mM imidazole. The cell suspension was lysed by stirring for 30 min at room temperature. The sol ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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