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Understanding the functional difference between growth
Understanding the functional difference between growth

... composed of 32 sequences with 314 sites. The graphical rendering of the alignment using JALVIEW 2.8 [53] is provided as electronic supplementary material, figure S1. Pairwise percentage identities were calculated using CLUSTALX. ...
Probing the conformational changes of the yeast mitochondrial ADP
Probing the conformational changes of the yeast mitochondrial ADP

... matrix. Access to the substrate binding site is regulated by salt bridge networks on either side of the central cavity, called the matrix and cytoplasmic salt bridge network. It has been proposed that during transport the salt bridge networks disrupt and form in an alternating way, opening and closi ...
Renal tubular reabsorption
Renal tubular reabsorption

... “downhill” transport of another substance • Carrier must be occupied by both substances (or be unoccupied) to be mobile in the membrane • Saturable (has a Vmax) • Demonstrates specificity and affinity of carrier for substance transported • “Uphill” transport occurs without direct input of ...
the RbDe web service - Oxford Academic
the RbDe web service - Oxford Academic

... diagrams and the creation of stored diagram libraries. The service has been tuned for the construction of snake-like diagrams (for transmembrane proteins) but can be used to render any protein for which defined secondary structure data or hypotheses are available. RbDe is freely available through th ...
tethering redox proteins to the outer membrane in Neisseria and
tethering redox proteins to the outer membrane in Neisseria and

... [15]. This yields a mature peptide with an N-terminal cysteine followed by an extended LCR (low-complexity region), which is in turn followed by a protein domain with high identity with the globular domains of homologous nitrite reductase, azurin and CCP respectively. {We have excluded cytochrome c ...
Protein Structure Prediction
Protein Structure Prediction

... Contribution of -turns in tertiary structure prediction of bioactive peptides • 3D structures of 77 biologically active peptides have been selected from PDB and other databases such as PSST (http://pranag.physics.iisc.ernet.in/psst) and PRF (http://www.genome.ad.jp/) have been selected. • The data ...
[BIO21] Biosynthetic production of human growth hormone
[BIO21] Biosynthetic production of human growth hormone

... scientists because it is found to not only retard biological aging, but also to significantly reverse many of the effects of aging (Klatz and Kahn, 1998). Besides 22K-hGH (22,000 Dalton), which is a major component composed of 191 amino acids, 20K-hGH (20,000 Dalton) is also known to be naturally se ...
Protein and Amino Acid Supplements
Protein and Amino Acid Supplements

... protein mass, muscle mass, physical function, immune function, or metabolic function. Additionally, the protein requirement depends on quality—that is AA composition and digestibility—and physical activity level. • It is more important than ever to be able to describe appropriately the protein and a ...
Full-Text
Full-Text

... conformations. A prion strain is characterized by its specific PrPTSE -conformation, the potential of infection and by PrPTSE spreading and deposition in the brain and other prion-associated tissues [20]. A high resolution structure for PrPTSE is not available and the conversion process is still not ...
A Drosophila Third Chromosome Minute Locus Encodes
A Drosophila Third Chromosome Minute Locus Encodes

... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
Cellular Internalization of Fluorescent Proteins via Arginine
Cellular Internalization of Fluorescent Proteins via Arginine

... materials across the biomembranes. Only specific biomolecules and ions may pass through via specific transporters or protein channels. Therefore, highly basic and hydrophilic AID peptides should not pass through the membranes. In fact, however, these AID peptides quite easily pass through the membra ...
Gene Section RBBP8 (retinoblastoma binding protein 8) Atlas of Genetics and Cytogenetics
Gene Section RBBP8 (retinoblastoma binding protein 8) Atlas of Genetics and Cytogenetics

... Domain structure of hCtIP. All known CtIP proteins contain a highly conserved CtBP-binding motif, two CXXC motifs and a phosphorylation site that corresponds to Ser327 in hCtIP. The Rb-binding motif is conserved only in mammalian CtIP. The N-terminal and C-terminal regions of all the CtIP homologs s ...
Transport Across Cell Membrane - Bioenergetics and Cell Metabolism
Transport Across Cell Membrane - Bioenergetics and Cell Metabolism

... Proteins that act as carriers are too large to move across the membrane. ...
PDF - World Wide Journals
PDF - World Wide Journals

... the presence of protein by biuret test. The more peptide bonds are in it, the higher the intensity of the purple colour. Absorbance is proportional to the concentration of a solution. So increasing the absorbance also shows higher protein concentration. Earlier researchers have observed and reported ...
Powerpoint Show on Dot Matrix
Powerpoint Show on Dot Matrix

... some criterion. • It does not refer to any historical process, just to a comparison of the sequences by some method. • It is a logically weaker statement. ...
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized

... the endoplasmic reticulum (ER) and other endomembranes (Sze et al. 1999). Plasma membrane H+-ATPase (AHA3) was recovered in fractions 18–21. Bip, an ER lumen marker protein, was also detected in the same fractions as AtMTP1 under the Mg2+-free condition (Fig. 2A). In the presence of Mg2+ (Fig. 2B), ...
Publications_files/Stotland et al 2012
Publications_files/Stotland et al 2012

... FACS-sorted, clonally selected on the basis of high mCherry expression, and probed with anti-CSN1 antibody to confirm the expression of SBP-CSN1 (Fig .1B–D). As a control, we utilized a similar construct where CSN1 was substituted with Citrine, a modified yellow fluorescent protein chosen to exclude ...
Team Publications - Centre de recherche de l`Institut Curie
Team Publications - Centre de recherche de l`Institut Curie

interaction of salinity and temperature on net protein synthesis and
interaction of salinity and temperature on net protein synthesis and

... environments where the effluence of freshwater lowers the salinity of the seawater. Thermal pollution of freshwaters may result in a large increase in the temperature of the nearshore water, and differences between fresh and seawaters may be even greater if cold nearshore waters have upwelled. Such ...
Working concentrations and stock solutions
Working concentrations and stock solutions

... 1. Prepare and autoclave/sterilize stock media. Be sure that the flask contains a stir-bar. 2. The solution must cool before adding antibiotics as the heat may inactive them. Let the flask equilibrate in the water bath set at 55-60o C for a minimum of 30 min. At this point, agar solutions should be ...
In Vitro Protein Expression Kit for Disulfide - Sigma
In Vitro Protein Expression Kit for Disulfide - Sigma

... We assume no liability for the following even within the warranty period: ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... Insertional disruption of the cysK gene did not produce a cysteine auxotroph, indicating that gene redundancy in the cysteine biosynthetic or other biosynthetic pathways exists in Azospirillum, as already described in other bacteria. Nitrogen fixation was not altered in the mutant strain as determin ...
A previously uncharacterized tetratricopeptide-repeat
A previously uncharacterized tetratricopeptide-repeat

(endosymbiotic) origin of the nucleus - Université Paris-Sud
(endosymbiotic) origin of the nucleus - Université Paris-Sud

... beyond any doubt. Endosymbiotic hypotheses need to explain the structural peculiarities of the nuclear membrane, but the origin of the nuclear membrane and its selective advantages are also puzzling for non-endosymbiotic supporters. Finally, Staub et al. argue that nucleus replication involves membr ...
Secretion Trap Tagging of Secreted and Membrane
Secretion Trap Tagging of Secreted and Membrane

... of ␤-Gal fusions with “trapped” N-terminal secretion signals (Skarnes et al., 1995). Sequencing of 5⬘-RACE products revealed that almost one-half encoded secreted proteins, with the remainder being expressed because of improper splicing or deletions of the vector (Townley et al., 1997). The techniqu ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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