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Protein Secondary Structure
Protein Secondary Structure

... What terminates an -helix? Statistically, a very high percentage (~60%) of helices are terminated by a single amino acid, Proline: ...
Protein Sequence and Structural Similarity
Protein Sequence and Structural Similarity

... Data Organelle nucleus, mitochondria, etc... (Proteomics) Which proteins are being produced in Nucleus which cells/tissues when? Which modified forms are present? ...
Identification and Characterization of Two Temperature
Identification and Characterization of Two Temperature

A Comparative Genomic Method for Computational
A Comparative Genomic Method for Computational

... – 9mers resembling binding site occur frequently in E. coli genome – not all sites matching consensus are actually bound by DnaA (ftsAQ) – some experimentally conserved binding sites differ from consensus – known DnaA regulated genes not functionally related (replication, lipid synthesis, house keep ...
Immunodetection of the expression of microsomal proteins encoded
Immunodetection of the expression of microsomal proteins encoded

... disease termed type 1b glycogen storage disease. The cDNA encoding a liver transporter for glucose 6-phosphate was cloned and was found to be mutated in patients suffering from glycogen storage disease 1b. While related mRNAs have been described in liver and other tissues, the encoded protein(s) has ...
- blogs@NTU
- blogs@NTU

... confirming the sensitivity of our approach, the purity of the vesicles and its ability to detect novel protein interactions. Vesicle tethering components of the SYP61 compartment A variety of proteins with a role in membrane trafficking were identified in the isolated SYP61 compartment, including YI ...
H 2 O Reabsorption
H 2 O Reabsorption

... ~70% of Filtrate is – Small plasma proteins reabsorbed in PCT – Some urea and uric acid Na+ is actively reabsorbed: First – simple diffusion: Then – 1o active transport: ...
The HSP90 family of genes in the human genome
The HSP90 family of genes in the human genome

Membrane Protein Expression in Cell
Membrane Protein Expression in Cell

... continuous development of new applications significantly promotes the distribution of this technology. Membrane protein synthesis by cell-free expression does not appear to be restricted by origin, size or topology of the target, and its global application is therefore a highly valuable characterist ...
Leishmania Flagellum
Leishmania Flagellum

... the tip of the flagellum [33]. Its main constituents are two structurally related proteins, PFR1 and PFR2 [33,34], which, as other cytoskeleton-associated proteins, remain insoluble after treatment with non-ionic detergents [35,36]. When expressed as fusion proteins with GFP at their N-terminus, PFR ...
BCL-2 Family Proteins: Critical Checkpoints of Apoptotic
BCL-2 Family Proteins: Critical Checkpoints of Apoptotic

... : gateway to the mitochondrial pathway of apoptosis 1. BAX and BAK fail to release cytochrome c 2. Activation of BAX AND BAK during apoptosis 3. Unlike BAX, BAK monomers are integrated into the MOM 4. Mitochondrial intramembranous homo-oligomerization of BAX & BAK is a prime candidate mechanism of M ...
Assembly and maintenance of the sarcomere night and day
Assembly and maintenance of the sarcomere night and day

... kinase domain, and also serves as a molecular spring.16 The N-terminus of titin spans the Z-disc and contains several small motifs termed z-repeats of 45 amino acids each, sandwiched between flanking regions containing Ig domains.17,18 Linking this region to GFP demonstrated that the z-repeats play ...
Identification, molecular characterization, and tissue
Identification, molecular characterization, and tissue

The effect of pH on the digestion of proteins in vitro by pepsin
The effect of pH on the digestion of proteins in vitro by pepsin

... It is evident from our results that all the proteins studied were most rapidly digested at pHs near z and that, although some proteolysis occurred at pH 4, there was no indication of a second peak of proteolytic activity at pH 3-5-4. There is general agreement that pepsin causes optimal proteolysis ...
Identification of a family of BspA like surface proteins of Entamoeba
Identification of a family of BspA like surface proteins of Entamoeba

y. Cell Set. Suppl. ¡1, 1-11 (1989) Printed in
y. Cell Set. Suppl. ¡1, 1-11 (1989) Printed in

... mitochondrial RNAs and a few (13 in humans) of the mitochondrial proteins. All the other hundreds of mitochondrial proteins, and probably also several mitochondrial RNAs, are encoded by nuclear genes and imported into the mitochondria. In spite of their genetic semi-autonomy, mitochondria are thus p ...
domaination
domaination

... (George & Heringa, Prot. Struct. Func. Genet., in press; 2002) ...
Does a backwardly read protein sequence have a unique native state?
Does a backwardly read protein sequence have a unique native state?

... the capping properties of helical termini (Presta and Rose, 1988; Richardson and Richardson, 1988). The first helix is fairly well capped at the N-terminus by Asnl2, and the Cterminus by His 19. The second helix N-cap Asn26-Glu25Glu26 is perfect, while the C-cap is marked only by the Lys36 residue. ...
Plant organelle proteomics
Plant organelle proteomics

... achieved. These methods are often referred to as shotgun proteomics. One such approach, MudPIT (multidimensional protein identification technology [20]), involves a solution phase digestion of proteins to peptides and then multi-dimensional chromatographic separation of peptides before mass spectrom ...
Strain TrpC TrpB TrpA
Strain TrpC TrpB TrpA

... polarity on TrpB and TrpA as in (B). However, this mutant reverts (regains TrpC activity) so this explanation is very unlikely). 4). One could consider an insertion mutation in trpC that also causes polarity. The fact that it does revert indicates that it could be an insertion. Since mutant #1 does ...
2.2 Membrane Structure and Functions
2.2 Membrane Structure and Functions

... and other components of one half of the lipid bilayer differ from those that make up the other half of the bilayer. This reflects the differences in the functions performed by each half of the membrane. For example, a range of glycolipids and carbohydrate groups attach to proteins on the external hal ...
A Few Good Domains
A Few Good Domains

... (Soluble NSF Attachment protein [SNAP] Receptors). SNARE proteins are believed to mediate most, if not all, cellular membrane fusion events. Most SNAREs are Cterminally anchored integral membrane proteins capable of entering into a coiled-coil interaction with other SNARE proteins. All SNARE protein ...
Molecular Components of the Signal Sequence that Function in the
Molecular Components of the Signal Sequence that Function in the

... One of the characteristic phenotypes exhibited by strains carrying the 42-1 fusion is related to the inability of the cell to export the LamB-LacZ hybrid protein efficiently. When cells containing this gene fusion are grown in the presence of maltose to induce high-level synthesis of the hybrid prot ...
The Structure and Topology of Protein Serine/Threonine
The Structure and Topology of Protein Serine/Threonine

... by four invariant aspartate residues and a non-conserved Glu residue (Figure 86.2b) [19]. These residues are situated at the top of the central -sandwich that forms a shallow channel suitable for the dephosphorylation of phosphoserine- and phosphothreonine-containing proteins. Six water molecules c ...
Slide 1
Slide 1

... Protein structure comparisons ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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