Two Functional a-Tubulin Genes of the Yeast Saccharomyces cerevisiae Encode Divergent Proteins.
... hybridizing bands (data not shown). Molecular cloning experiments yielded two genes, henceforth called TUB] and TUB3 (the TUB2 gene encodes P-tubulin). The TUB3 gene was isolated from a library of partially Sau3A-digested genomic DNA in the vector XBF101 by plaque hybridization (7) and subcloned int ...
... hybridizing bands (data not shown). Molecular cloning experiments yielded two genes, henceforth called TUB] and TUB3 (the TUB2 gene encodes P-tubulin). The TUB3 gene was isolated from a library of partially Sau3A-digested genomic DNA in the vector XBF101 by plaque hybridization (7) and subcloned int ...
lecture08_11
... – Detect similar function where sequence differs (only ~50% remote homologies can be detected based on sequence) ...
... – Detect similar function where sequence differs (only ~50% remote homologies can be detected based on sequence) ...
297 special transport and neurological significance of two amino
... would allow the D-form of both amino acids to present its amino group in the orientation characteristic of the natural L-form. The dual modes of recognition should accordingly be signalled by what appears to be low stereospecificity, actually due to a distinction in the enantiorecognition of the two ...
... would allow the D-form of both amino acids to present its amino group in the orientation characteristic of the natural L-form. The dual modes of recognition should accordingly be signalled by what appears to be low stereospecificity, actually due to a distinction in the enantiorecognition of the two ...
HRT Gene Function Requires Interaction between a NAC Protein
... First, most of the characterized R gene products share structural motifs. Among them, nucleotide binding sites (NBS) and leucine-rich repeats (LRR) have been most commonly described. TIP lacks both NBS and LRR motifs. However, the product of HRT, a newly characterized gene involved in the TCV resist ...
... First, most of the characterized R gene products share structural motifs. Among them, nucleotide binding sites (NBS) and leucine-rich repeats (LRR) have been most commonly described. TIP lacks both NBS and LRR motifs. However, the product of HRT, a newly characterized gene involved in the TCV resist ...
107105_pku
... ~125,000 base pairs codes for a protein of 454 amino acids only 1362 base pairs code for amino ac ids 13 exons containing 41-184 base pairs coding for protein 12 introns from 1, 200 to 23,500 base pairs in length ...
... ~125,000 base pairs codes for a protein of 454 amino acids only 1362 base pairs code for amino ac ids 13 exons containing 41-184 base pairs coding for protein 12 introns from 1, 200 to 23,500 base pairs in length ...
HOW TO USE BAD: A BRIEF PRESENTATION
... Critical to the BAD project was the open and free access to the database, as well as the possibility of continuous upgrade, either by database maintenance and inclusion of newly published data, or entries submitted by individual researchers. We have organised and implemented the BAD on a MS-Access p ...
... Critical to the BAD project was the open and free access to the database, as well as the possibility of continuous upgrade, either by database maintenance and inclusion of newly published data, or entries submitted by individual researchers. We have organised and implemented the BAD on a MS-Access p ...
Derived copy of Bis2A 14.1 Bacterial Gene
... process these alternate genes must be transcribed. When glucose levels drop, cyclic AMP (cAMP) begins to accumulate in the cell. The cAMP molecule is a signaling molecule that is involved in glucose and energy metabolism in E. coli. When glucose levels decline in the cell, accumulating cAMP binds to ...
... process these alternate genes must be transcribed. When glucose levels drop, cyclic AMP (cAMP) begins to accumulate in the cell. The cAMP molecule is a signaling molecule that is involved in glucose and energy metabolism in E. coli. When glucose levels decline in the cell, accumulating cAMP binds to ...
Study Questions for Chapter 1 – The Cell
... 4. When plotting the velocity (V) of an enzymatic reaction against the substrate concentration, one sees “saturable” kinetics. That is, at some substrate concentration, the enzyme is functioning at its maximal rate (Vmax) and cannot operate any faster. The substrate concentration that results in ...
... 4. When plotting the velocity (V) of an enzymatic reaction against the substrate concentration, one sees “saturable” kinetics. That is, at some substrate concentration, the enzyme is functioning at its maximal rate (Vmax) and cannot operate any faster. The substrate concentration that results in ...
An overview of E. coli expression systems
... E.g. CGT, CGC, CGG, AGG, AGA, CGA code for arginine, but the last 3 (AGG, AGA, CGA) are rarely used in E. coli and it has low amounts of respective tRNAs. ...
... E.g. CGT, CGC, CGG, AGG, AGA, CGA code for arginine, but the last 3 (AGG, AGA, CGA) are rarely used in E. coli and it has low amounts of respective tRNAs. ...
Chapter 9 Proteins - Angelo State University
... Peptides • Peptides are named by starting at the N-terminal end and listing the amino acid residues from left to right. • Large amino acid chains are unwieldy to draw in their complete forms, so they are usually represented by their three-letter abbreviations, separated by dashes: – Gly-Ala (Gly = N ...
... Peptides • Peptides are named by starting at the N-terminal end and listing the amino acid residues from left to right. • Large amino acid chains are unwieldy to draw in their complete forms, so they are usually represented by their three-letter abbreviations, separated by dashes: – Gly-Ala (Gly = N ...
An Expression and Bioinformatics Analysis of the Arabidopsis
... At3g12240 contains an aberrant carboxyterminal region. Examination of the corresponding genomic sequence revealed that the 3# end of the penultimate exon of the gene was not identified correctly during annotation, resulting in the inferred translation continuing through the final intron until a stop ...
... At3g12240 contains an aberrant carboxyterminal region. Examination of the corresponding genomic sequence revealed that the 3# end of the penultimate exon of the gene was not identified correctly during annotation, resulting in the inferred translation continuing through the final intron until a stop ...
Design and Evolution of Artificial M13 Coat Proteins
... coat predominantly composed of wild-type proteins supplied in trans from a helper phage (Bass et al., 1990). In such systems, the helper phage supplies all the proteins necessary for viral assembly, and thus, the fusion gene product is a nonfunctional coat protein that incorporates into the phage pa ...
... coat predominantly composed of wild-type proteins supplied in trans from a helper phage (Bass et al., 1990). In such systems, the helper phage supplies all the proteins necessary for viral assembly, and thus, the fusion gene product is a nonfunctional coat protein that incorporates into the phage pa ...
Membrane trafficking in Drosophila wing and eye development
... signaling cascade is endocytosis of the Notch extracellular domain into the ligand expressing cell.8 This process is called trans-endocytosis. While the dynamics of Delta trafficking have been known for some time,9 and that improper trafficking of Delta leads to impairment of Notch signaling,9 the dir ...
... signaling cascade is endocytosis of the Notch extracellular domain into the ligand expressing cell.8 This process is called trans-endocytosis. While the dynamics of Delta trafficking have been known for some time,9 and that improper trafficking of Delta leads to impairment of Notch signaling,9 the dir ...
Investigation of Iron-Sulfur Protein Maturation in Eukaryotes
... cultures or of pathogenic protists, experiments may be complicated because of low amounts of available sample. Nevertheless, most techniques used in yeast can be applied in principle. Wherever possible, we provide assays that have been established in our laboratory for the analysis of Fe-S protein b ...
... cultures or of pathogenic protists, experiments may be complicated because of low amounts of available sample. Nevertheless, most techniques used in yeast can be applied in principle. Wherever possible, we provide assays that have been established in our laboratory for the analysis of Fe-S protein b ...
1 -2 -2 2 -3 I -1
... Galton left his home and headed for a country fair… He believed that only a very few people had the characteristics necessary to keep societies healthy. He had devoted much of his career to measuring those characteristics, in fact, in order to prove that the vast majority of people did not have them ...
... Galton left his home and headed for a country fair… He believed that only a very few people had the characteristics necessary to keep societies healthy. He had devoted much of his career to measuring those characteristics, in fact, in order to prove that the vast majority of people did not have them ...
An Efficient Protocol for Identifying Separation-of-Function
... ABSTRACT Mutations that confer the loss of a single biochemical property (separation-of-function mutations) can often uncover a previously unknown role for a protein in a particular biological process. However, most mutations are identified based on loss-offunction phenotypes, which cannot differenti ...
... ABSTRACT Mutations that confer the loss of a single biochemical property (separation-of-function mutations) can often uncover a previously unknown role for a protein in a particular biological process. However, most mutations are identified based on loss-offunction phenotypes, which cannot differenti ...
Nuclear Localization and Interaction with COP1 Are Required for
... system (Holm et al., 2002) and colocalizes with it in plant cells (Indorf et al., 2007). It contains two B-box Zn fingers situated in tandem in the N-terminal part of the protein, whereas at the C terminus, several amino acid residues necessary for the interaction with COP1 have been identified (Hol ...
... system (Holm et al., 2002) and colocalizes with it in plant cells (Indorf et al., 2007). It contains two B-box Zn fingers situated in tandem in the N-terminal part of the protein, whereas at the C terminus, several amino acid residues necessary for the interaction with COP1 have been identified (Hol ...
Introduction
... and proteins involved in the absorption of nutrients. It contains receptors for transferrin, lipoproteins and growth factors, and has the same functions as the membrane in non-polarised cells. This membrane domain is also responsible for adhesion of the cell to the extracellular matrix, and contains ...
... and proteins involved in the absorption of nutrients. It contains receptors for transferrin, lipoproteins and growth factors, and has the same functions as the membrane in non-polarised cells. This membrane domain is also responsible for adhesion of the cell to the extracellular matrix, and contains ...
Document
... group gives a complex titration curve with two inflection points. 3. More complex amino acids with an ionizable R group show even more complex titration curves. ...
... group gives a complex titration curve with two inflection points. 3. More complex amino acids with an ionizable R group show even more complex titration curves. ...
A “Tag-and-Modify” Approach to Site
... generate a mixed disulfide after air oxidation. However, under such conditions unwanted symmetrical disulfides can form. In contrast, reagents that bear methanethiosulfonate (MTS),24,25 phenylthiosulfonate (PTS),26 or phenylselenenylsulfide (SeS),27 react rapidly and specifically with cysteine to pr ...
... generate a mixed disulfide after air oxidation. However, under such conditions unwanted symmetrical disulfides can form. In contrast, reagents that bear methanethiosulfonate (MTS),24,25 phenylthiosulfonate (PTS),26 or phenylselenenylsulfide (SeS),27 react rapidly and specifically with cysteine to pr ...
Continued..
... When a protein sustains about 250 hits per 100 amino acids, it may have about 20% identity with the original protein, and it can still be recognizable as significantly related. If a protein sustains 360 changes per 100 residues, it evolves to a point at which the two proteins share about 15% ami ...
... When a protein sustains about 250 hits per 100 amino acids, it may have about 20% identity with the original protein, and it can still be recognizable as significantly related. If a protein sustains 360 changes per 100 residues, it evolves to a point at which the two proteins share about 15% ami ...
Document
... frameshift mutations by inserting or deleting base pairs of DNA. The reasoning was that reversion of a deletion (a -mutation) could be caused by a nearby insertion (a +mutation), and vice versa. Revertants of rII to r+ can be detected by plaques on E. coli K12(l). ...
... frameshift mutations by inserting or deleting base pairs of DNA. The reasoning was that reversion of a deletion (a -mutation) could be caused by a nearby insertion (a +mutation), and vice versa. Revertants of rII to r+ can be detected by plaques on E. coli K12(l). ...