Ion Exchange Chromatography
... The target analytes (anions or cations) are retained on the stationary phase but can be eluted by increasing the concentration of a similarly charged species that will displace the analyte ions from the stationary phase. For example, in cation exchange chromatography, the positively charged analyte ...
... The target analytes (anions or cations) are retained on the stationary phase but can be eluted by increasing the concentration of a similarly charged species that will displace the analyte ions from the stationary phase. For example, in cation exchange chromatography, the positively charged analyte ...
Protein digestion in poultry – the value of an
... hydrolysis of incoming proteins, absorption, further processing and the concurrent secretion and recovery of endogenous protein • Endogenous proteins are often less well recovered that exogenous proteins and ProAct may assist the animals with digestion of both fractions • Though ProAct currently del ...
... hydrolysis of incoming proteins, absorption, further processing and the concurrent secretion and recovery of endogenous protein • Endogenous proteins are often less well recovered that exogenous proteins and ProAct may assist the animals with digestion of both fractions • Though ProAct currently del ...
doc - DePaul University
... the overall stability of the protein while the non-core amino acids play a significant role in determining the tertiary structure. The results were encouraging, demonstrating that it was possible to repack the core alone with novel sequences. Unfortunately, the potential sequences were limited by th ...
... the overall stability of the protein while the non-core amino acids play a significant role in determining the tertiary structure. The results were encouraging, demonstrating that it was possible to repack the core alone with novel sequences. Unfortunately, the potential sequences were limited by th ...
as a PDF
... previously described above. Fd was further purified by gel filtration on a pre-packed Superdex 75 HR 10/30 column coupled to a HiLoad 26/10 ‘‘Fast flow column’’, both from Amersham Biosciences and previously equilibrated with 50 mM Phosphate buffer (pH 7.6) containing 150 mM NaCl. Fd fractions were ...
... previously described above. Fd was further purified by gel filtration on a pre-packed Superdex 75 HR 10/30 column coupled to a HiLoad 26/10 ‘‘Fast flow column’’, both from Amersham Biosciences and previously equilibrated with 50 mM Phosphate buffer (pH 7.6) containing 150 mM NaCl. Fd fractions were ...
CD - Instituto de Investigaciones Biotecnológicas
... NQ-Flipper - Validation and correction of asparagine and glutamine side-chain amide rotamers in protein structures solved by X-ray crystallography PROCHECK - Verification of the stereochemical quality of a protein structure ProSA-web - Recognition of errors in 3D structures of proteins QMEAN - Serve ...
... NQ-Flipper - Validation and correction of asparagine and glutamine side-chain amide rotamers in protein structures solved by X-ray crystallography PROCHECK - Verification of the stereochemical quality of a protein structure ProSA-web - Recognition of errors in 3D structures of proteins QMEAN - Serve ...
Investigation of the starch-binding properties of wheat friabilin
... 15kDa (under reducing conditions). The molecular weight calculated from the DNA sequences is 14kDa for both PA and GSPP. The difference between the calculated and apparent molecular weights of PA and GSPP might be caused by the large number of basic amino acid residues present in these polypeptides. ...
... 15kDa (under reducing conditions). The molecular weight calculated from the DNA sequences is 14kDa for both PA and GSPP. The difference between the calculated and apparent molecular weights of PA and GSPP might be caused by the large number of basic amino acid residues present in these polypeptides. ...
CS790 – Introduction to Bioinformatics
... Disulfide Bonds Two cyteines in close proximity will form a covalent bond Disulfide bond, disulfide bridge, or dicysteine bond. Significantly stabilizes tertiary structure. Protein Folding ...
... Disulfide Bonds Two cyteines in close proximity will form a covalent bond Disulfide bond, disulfide bridge, or dicysteine bond. Significantly stabilizes tertiary structure. Protein Folding ...
SOD binds cell-adhesive peroxidase - Journal of Cell Science
... showing that the staining was not due to non-specific binding of peroxinectin; no staining was seen when the antiperoxinectin antibodies were omitted or replaced by preimmune serum (not shown). Incubation of cells with antiperoxinectin antibodies without prior incubation with peroxinectin did not re ...
... showing that the staining was not due to non-specific binding of peroxinectin; no staining was seen when the antiperoxinectin antibodies were omitted or replaced by preimmune serum (not shown). Incubation of cells with antiperoxinectin antibodies without prior incubation with peroxinectin did not re ...
Breakfast Computations Simplified
... Production of unnatural molecules is inevitable. Naturally occurring amino acids undergo isomeric changes (changes in shape morphing) as well as transformation into toxic forms, under the impact of microwaves. The violent change that microwaving causes to food microstructure forms new molecular form ...
... Production of unnatural molecules is inevitable. Naturally occurring amino acids undergo isomeric changes (changes in shape morphing) as well as transformation into toxic forms, under the impact of microwaves. The violent change that microwaving causes to food microstructure forms new molecular form ...
Chlamydomonas reinhardtii strains carrying the stb1-1
... The original mutant strains I73 (sta4-1) and I97 (sta4-2) were transformed with the complete PHOB genomic fragment amplified by PCR and cloned in the pSL18 plasmid (see methods). The paromomycin resistant clones were screened by zymogram (a). Three complementated strains were obtained from 23 resist ...
... The original mutant strains I73 (sta4-1) and I97 (sta4-2) were transformed with the complete PHOB genomic fragment amplified by PCR and cloned in the pSL18 plasmid (see methods). The paromomycin resistant clones were screened by zymogram (a). Three complementated strains were obtained from 23 resist ...
Transport of Aromatic Amino Acids by Brevibacterium linens
... than 38% (Table 1). It would appear that the aamino and a-carboxyl are less important in this case than for the transport of the other two aromatic amino acids, since these functional groups were absent from potent inhibitors. The length of the carbon chain on the indole ring also seems to be of sec ...
... than 38% (Table 1). It would appear that the aamino and a-carboxyl are less important in this case than for the transport of the other two aromatic amino acids, since these functional groups were absent from potent inhibitors. The length of the carbon chain on the indole ring also seems to be of sec ...
The IML3/ MCM19 gene of Saccharomyces cerevisiae is required
... DNA-binding domain (BD) in pGBT9 and with the Gal4 activation domain (AD) in pGAD424 (Bartel et al. 1993). A BamHI site was designed in the ATG primer (the primer used to amplify the ORF from its ®rst codon), so that the BamHI site of pGBT9 or pGAD424 could be used for fusing the ORF in frame with t ...
... DNA-binding domain (BD) in pGBT9 and with the Gal4 activation domain (AD) in pGAD424 (Bartel et al. 1993). A BamHI site was designed in the ATG primer (the primer used to amplify the ORF from its ®rst codon), so that the BamHI site of pGBT9 or pGAD424 could be used for fusing the ORF in frame with t ...
CS790 – Introduction to Bioinformatics
... Disulfide Bonds Two cyteines in close proximity will form a covalent bond Disulfide bond, disulfide bridge, or dicysteine bond. Significantly stabilizes tertiary structure. Protein Folding ...
... Disulfide Bonds Two cyteines in close proximity will form a covalent bond Disulfide bond, disulfide bridge, or dicysteine bond. Significantly stabilizes tertiary structure. Protein Folding ...
Evolution of the eukaryotic membrane
... In this article we use the traditional, evolutionary biological, definition of homology: that two homologous objects share common ancestry, i.e. were once the same object in the common ancestor of the organisms that are being considered (Reeck et al., 1987). This definition can be applied to appenda ...
... In this article we use the traditional, evolutionary biological, definition of homology: that two homologous objects share common ancestry, i.e. were once the same object in the common ancestor of the organisms that are being considered (Reeck et al., 1987). This definition can be applied to appenda ...
Breakfast Computations Simplified
... Production of unnatural molecules is inevitable. Naturally occurring amino acids undergo isomeric changes (changes in shape morphing) as well as transformation into toxic forms, under the impact of microwaves. The violent change that microwaving causes to food microstructure forms new molecular form ...
... Production of unnatural molecules is inevitable. Naturally occurring amino acids undergo isomeric changes (changes in shape morphing) as well as transformation into toxic forms, under the impact of microwaves. The violent change that microwaving causes to food microstructure forms new molecular form ...
Development of Amino Acid as Parenteral Nutrition P N
... Protein is the most abundant nitrogen-containing compound in the diet and in the human body. It is one of the complex bio-molecules present in cells and tissues. All amino acids (AAs) have a similar chemical structure-each contains an amino group (NH2), an acid group (COOH), a hydrogen atom (H), and ...
... Protein is the most abundant nitrogen-containing compound in the diet and in the human body. It is one of the complex bio-molecules present in cells and tissues. All amino acids (AAs) have a similar chemical structure-each contains an amino group (NH2), an acid group (COOH), a hydrogen atom (H), and ...
Protein import into the nucleus - Universitätsklinikum des Saarlandes
... of macromolecules between the cytoplasm and the nucleoplasm. This review will focus on new findings on protein import into the nucleus. Export of proteins and import/export of R N A have been reviewed recently [1-4]. Molecules of up to ,~ 50 kDa may pass the NPC by diffusion. However, even small pro ...
... of macromolecules between the cytoplasm and the nucleoplasm. This review will focus on new findings on protein import into the nucleus. Export of proteins and import/export of R N A have been reviewed recently [1-4]. Molecules of up to ,~ 50 kDa may pass the NPC by diffusion. However, even small pro ...
The glutamate receptor of the Qp-type activates protein kinase C
... [~-32p]ATP (spec. act. 300 cpm/pmol) and 20/21 of soluble or particulate fractions. The reaction was initiated by addition of the [7-32p]ATP. After 5 min of incubation, a 50/~l aliquot was spotted on Whatman P81 cation exchange paper. Papers were washed 3 times in ice-cold water, dried and counted b ...
... [~-32p]ATP (spec. act. 300 cpm/pmol) and 20/21 of soluble or particulate fractions. The reaction was initiated by addition of the [7-32p]ATP. After 5 min of incubation, a 50/~l aliquot was spotted on Whatman P81 cation exchange paper. Papers were washed 3 times in ice-cold water, dried and counted b ...
Directed Evolution of ATP Binding Proteins from a Zinc Finger
... commonly seen in many ATP binding proteins [34]. Alternatively, some of these residues may play a structural role in stabilizing the overall protein fold. It will be interesting to compare selections for different ligands by using this RXR library to see if the same fixed positions are observed agai ...
... commonly seen in many ATP binding proteins [34]. Alternatively, some of these residues may play a structural role in stabilizing the overall protein fold. It will be interesting to compare selections for different ligands by using this RXR library to see if the same fixed positions are observed agai ...
Translation - Santa Susana High School
... – mRNA moves from 5' to 3' – AAs are added N-terminus to C-terminus with new AAs added to the carboxyl end – elongation ends when a stop is reached - UAG, UAA, UGA • do not code for an AA but binds to a release factor that binds to the adjacent A-site • the release factor binds a water molecule to t ...
... – mRNA moves from 5' to 3' – AAs are added N-terminus to C-terminus with new AAs added to the carboxyl end – elongation ends when a stop is reached - UAG, UAA, UGA • do not code for an AA but binds to a release factor that binds to the adjacent A-site • the release factor binds a water molecule to t ...
Journal of Bacteriology
... The nitrogenase of the free-living, microaerobic, N2-fixing bacterium AzospiriUum amazonense (strain Y1) was purified by chromatography on DEAE-52 cellulose, by heat treatment, and by preparative polyacrylamide gel electrophoresis. The specific nitrogenase activities were 2,400 nmol of C21[4 formed ...
... The nitrogenase of the free-living, microaerobic, N2-fixing bacterium AzospiriUum amazonense (strain Y1) was purified by chromatography on DEAE-52 cellulose, by heat treatment, and by preparative polyacrylamide gel electrophoresis. The specific nitrogenase activities were 2,400 nmol of C21[4 formed ...
Full_ppt_ch20
... – An amino acid that contains a second carboxyl group in its side chain – R = –CH2COOH, or -COOH ...
... – An amino acid that contains a second carboxyl group in its side chain – R = –CH2COOH, or -COOH ...