Incomplete citric acid cycle obliges aminolevulinic
... formation of oxaloacetate in 20 mwpotassium phosphate (pH 7 . 9 , 0.5 mM-NAD+ or 0.2 mM-NADP', 0.2 mM-acetyl-CoA, 0.1 m ~ - 5 , 5 ' dithiobis(2-nitrobenzoic acid), 5.8 U porcine-heart citrate synthase and 1 mwmalate. 2-Oxoglutarate decarboxylase was monitored by coupling the thiamin pyrophosphate an ...
... formation of oxaloacetate in 20 mwpotassium phosphate (pH 7 . 9 , 0.5 mM-NAD+ or 0.2 mM-NADP', 0.2 mM-acetyl-CoA, 0.1 m ~ - 5 , 5 ' dithiobis(2-nitrobenzoic acid), 5.8 U porcine-heart citrate synthase and 1 mwmalate. 2-Oxoglutarate decarboxylase was monitored by coupling the thiamin pyrophosphate an ...
Feodor Lynen - Nobel Lecture
... The recognition that acetyl-CoA is a thio ester was of the utmost importance in another respect. It was already known from investigations on the degradation of fatty acids by mitochondria from liver or bacterial extracts that the acids must be "activated" before the ,9 -oxidation can begin (cf. ref. ...
... The recognition that acetyl-CoA is a thio ester was of the utmost importance in another respect. It was already known from investigations on the degradation of fatty acids by mitochondria from liver or bacterial extracts that the acids must be "activated" before the ,9 -oxidation can begin (cf. ref. ...
Enzymes
... known physiological function in the plasma 1) Some are secreted into the plasma 2) A number of enzymes associated with cell metabolism normally found in the plasma only in low concentrations. – An increased plasma concentration of these enzymes is associated with cell disruption or death ...
... known physiological function in the plasma 1) Some are secreted into the plasma 2) A number of enzymes associated with cell metabolism normally found in the plasma only in low concentrations. – An increased plasma concentration of these enzymes is associated with cell disruption or death ...
Physiology of a New Facultatively Autotrophic
... provided by T. D. Brock in August, 1968. The samples were well shaken and 5 ml of each was inoculated into 25 ml sulphur medium, pH 7.0 and incubated at 50' for 7 days. Of five enrichments, three showed distinct growth of similar Gram-negative non-motile, non-sporeforming rods. The organism describe ...
... provided by T. D. Brock in August, 1968. The samples were well shaken and 5 ml of each was inoculated into 25 ml sulphur medium, pH 7.0 and incubated at 50' for 7 days. Of five enrichments, three showed distinct growth of similar Gram-negative non-motile, non-sporeforming rods. The organism describe ...
Subroto Chatterjee CV
... 67. Chatterjee S and Wei HM. Roles of Glycosphingolipids in cell signaling: Adhesion, migration and proliferation. Methods in Enzymology 363: 300-312 2003. 68. Martin S and Chatterjee S. Methods to study glycosphingolipids in signal transduction: apoptosis. Methods in Enzymology 363: 284-299 2003. 6 ...
... 67. Chatterjee S and Wei HM. Roles of Glycosphingolipids in cell signaling: Adhesion, migration and proliferation. Methods in Enzymology 363: 300-312 2003. 68. Martin S and Chatterjee S. Methods to study glycosphingolipids in signal transduction: apoptosis. Methods in Enzymology 363: 284-299 2003. 6 ...
Divergent evolution of the thiolase superfamily and chalcone
... Enzymes of the thiolase superfamily catalyze the formation of carbon–carbon bond via the Claisen condensation reaction. Thiolases catalyze the reversible non-decarboxylative condensation of acetoacetyl-CoA from two molecules of acetyl-CoA, and possess a conserved Cys–His catalytic diad. Elongation e ...
... Enzymes of the thiolase superfamily catalyze the formation of carbon–carbon bond via the Claisen condensation reaction. Thiolases catalyze the reversible non-decarboxylative condensation of acetoacetyl-CoA from two molecules of acetyl-CoA, and possess a conserved Cys–His catalytic diad. Elongation e ...
Phylogenomic Investigation of Phospholipid Synthesis in Archaea
... distinctive archaeal MVA pathway in the last archaeal common ancestor (LACA) and support that different isoprenoid precursor biosynthesis pathways are characteristic of each domain of life: the classical MVA pathway in eukaryotes, the alternative MVA pathway in archaea, and the MEP pathway in bacteri ...
... distinctive archaeal MVA pathway in the last archaeal common ancestor (LACA) and support that different isoprenoid precursor biosynthesis pathways are characteristic of each domain of life: the classical MVA pathway in eukaryotes, the alternative MVA pathway in archaea, and the MEP pathway in bacteri ...
Full Text - the American Society of Animal Science
... positive correlation coefficients were found between the FABP-4 protein level and adipocyte number (R2 = 0.47, P = 0.02) and lipid content (R2 = 0.58, P = 0.004). Conversely, there was no difference between groups relative to FABP-3 mRNA (P = 0.46) or protein (P = 0.56) levels, oxidative enzymatic a ...
... positive correlation coefficients were found between the FABP-4 protein level and adipocyte number (R2 = 0.47, P = 0.02) and lipid content (R2 = 0.58, P = 0.004). Conversely, there was no difference between groups relative to FABP-3 mRNA (P = 0.46) or protein (P = 0.56) levels, oxidative enzymatic a ...
1 Development of Bombesin Analogues With Conformationally
... JPET Fast Forward. Published on April 21, 2004 as DOI: 10.1124/jpet.104.066761 This article has not been copyedited and formatted. The final version may differ from this version. ...
... JPET Fast Forward. Published on April 21, 2004 as DOI: 10.1124/jpet.104.066761 This article has not been copyedited and formatted. The final version may differ from this version. ...
galanin - Personal Home Pages (at UEL)
... •Only one galanin receptor subtype has been cloned so far - GalR1. •Pharmacological evidence suggests more than one receptor subtype exists. ...
... •Only one galanin receptor subtype has been cloned so far - GalR1. •Pharmacological evidence suggests more than one receptor subtype exists. ...
Pancreatic Hormones and Insulin Receptor Agonists
... The bulk of the pancreas is an exocrine gland secreting pancreatic fluid into the duodenum after a meal. Inside the pancreas are millions of clusters of cells called islets of Langerhans. The islets are endocrine tissue containing four types of cells. In order of abundance, they are: beta cells, wh ...
... The bulk of the pancreas is an exocrine gland secreting pancreatic fluid into the duodenum after a meal. Inside the pancreas are millions of clusters of cells called islets of Langerhans. The islets are endocrine tissue containing four types of cells. In order of abundance, they are: beta cells, wh ...
phys chapter 78 [2-9
... o Excess of citrate and isocitrate ions formed by TCA cycle when excess amounts of glucose being used for energy; ions have direct effect in activating acetyl-CoA carboxylase (required for first stage of fatty acid synthesis) o Most of fatty acids synthesized in liver and used to form triglycerides; ...
... o Excess of citrate and isocitrate ions formed by TCA cycle when excess amounts of glucose being used for energy; ions have direct effect in activating acetyl-CoA carboxylase (required for first stage of fatty acid synthesis) o Most of fatty acids synthesized in liver and used to form triglycerides; ...
biochemical investigation into initiation of fatty acid synthesis in the
... expression and activity is regulated by phosphorylation in response to altered lipid environments. In the third chapter, which has been published, we tested the effects of a compound in green tea extract known as epigallocatechin gallate (EGCG), on growth of T. brucei, and on TbACC activity and phos ...
... expression and activity is regulated by phosphorylation in response to altered lipid environments. In the third chapter, which has been published, we tested the effects of a compound in green tea extract known as epigallocatechin gallate (EGCG), on growth of T. brucei, and on TbACC activity and phos ...
The Incorporation of Glycerol and Lysine into the Lipid Fraction of
... 1. Incubation of washed cells of Staphylococcus aureus with [1-14C]glycerol results in the incorporation of glycerol into the lipid fraction of the cells. The rate of incorporation is increased by the presence of glucose and amino acids. The presence ofamino acids increases incorporation into the fr ...
... 1. Incubation of washed cells of Staphylococcus aureus with [1-14C]glycerol results in the incorporation of glycerol into the lipid fraction of the cells. The rate of incorporation is increased by the presence of glucose and amino acids. The presence ofamino acids increases incorporation into the fr ...
Transcriptional Regulation of Metabolism
... briefly presented. Appendix A presents the nuclear receptor family and is accompanied by Figure 1A, which shows the main characteristics of the transcription factors belonging to this family. The notion of “metabolic sensor” receptors was more particularly developed with respect to these nuclear rec ...
... briefly presented. Appendix A presents the nuclear receptor family and is accompanied by Figure 1A, which shows the main characteristics of the transcription factors belonging to this family. The notion of “metabolic sensor” receptors was more particularly developed with respect to these nuclear rec ...
Dietary Fat Composition Influences Tissue Lipid Profile and Gene
... was quantified by the Bradford assay. The microsomal fractions were stored in aliquots at -80 oC. A fluorescent DGAT assay was carried out as described by McFie and Stone [25]. Briefly, a master mix was prepared in a test tube containing 20 ll of 1 M Tris–HCl (pH 7.6), 4 ll of 1 M MgCl2, 10 ll of 4 mM ...
... was quantified by the Bradford assay. The microsomal fractions were stored in aliquots at -80 oC. A fluorescent DGAT assay was carried out as described by McFie and Stone [25]. Briefly, a master mix was prepared in a test tube containing 20 ll of 1 M Tris–HCl (pH 7.6), 4 ll of 1 M MgCl2, 10 ll of 4 mM ...
Biosynthesis of Lipids and Hydrocarbons in Algae
... et al., 2011). In mammalian mitochondria, the reversal pathway functions for only elongation process due to substrate specificity of mammal enoyl-CoA reductase and therefore the pathway contributes to short-chain fatty acid elongation process (Inui et al., 1984). 2.2. Fatty acid elongation C18-Fatty ...
... et al., 2011). In mammalian mitochondria, the reversal pathway functions for only elongation process due to substrate specificity of mammal enoyl-CoA reductase and therefore the pathway contributes to short-chain fatty acid elongation process (Inui et al., 1984). 2.2. Fatty acid elongation C18-Fatty ...
Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid
... primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal ca ...
... primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal ca ...
Regulation of the heat stress response in Arabidopsis by
... 2004; Bethke et al., 2011), little is yet known about the specific functions of most other group B and even less is known on group C or group D members, except that group C members (MPK1, 2, 7 and 14) and at least one group D member (MPK8) are downstream of MKK3 which plays a role in pathogen and ox ...
... 2004; Bethke et al., 2011), little is yet known about the specific functions of most other group B and even less is known on group C or group D members, except that group C members (MPK1, 2, 7 and 14) and at least one group D member (MPK8) are downstream of MKK3 which plays a role in pathogen and ox ...
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in
... families composed of enzymes that show similar substrate specificities and modes of regulation (63). This review focuses on bacterial kinases with catalytic domains that share structural and functional homology with eukaryotic Ser/Thr kinases. These kinases are referred to herein as eSTKs to disting ...
... families composed of enzymes that show similar substrate specificities and modes of regulation (63). This review focuses on bacterial kinases with catalytic domains that share structural and functional homology with eukaryotic Ser/Thr kinases. These kinases are referred to herein as eSTKs to disting ...
Міністерство охорони здоров`я України Харківський
... metabolism of basic classes of biomolecules (proteins, amino acids, nucleic acids, nucleotides, carbohydrates, lipids), regulation of metabolism and physiologic functions, biochemistry of enzymes, vitamins, blood, kidney, muscle, liver, immune, nervous, connective tissues. Modern conceptions of mole ...
... metabolism of basic classes of biomolecules (proteins, amino acids, nucleic acids, nucleotides, carbohydrates, lipids), regulation of metabolism and physiologic functions, biochemistry of enzymes, vitamins, blood, kidney, muscle, liver, immune, nervous, connective tissues. Modern conceptions of mole ...
Hyaluronic acid influence on platelet-induced airway smooth muscle cell proliferation
... different structural changes, such as increased cell number (goblet cells, fibroblasts and airway smooth muscle cells (ASMC)), cause airway remodeling. Platelets have been shown to interact with different cell types, e.g. by recruiting leucocytes through P-selectin in a murine model of chronic aller ...
... different structural changes, such as increased cell number (goblet cells, fibroblasts and airway smooth muscle cells (ASMC)), cause airway remodeling. Platelets have been shown to interact with different cell types, e.g. by recruiting leucocytes through P-selectin in a murine model of chronic aller ...
A Study of the Membrane–Water Interface Region of Membrane
... membrane proteins in the study. The global view In agreement with earlier findings,2 the secondary structure composition in the G10 Å central region of the membrane is almost 100% helix, Figure 1(a). The helix content steadily decreases from the center of the membrane to a minimum at around G35 Å. ...
... membrane proteins in the study. The global view In agreement with earlier findings,2 the secondary structure composition in the G10 Å central region of the membrane is almost 100% helix, Figure 1(a). The helix content steadily decreases from the center of the membrane to a minimum at around G35 Å. ...
- World Journal of Gastroenterology
... oxalacetic transaminase (GOT or AST) were elevated after acute myocardial infarction[2]. It is noteworthy to mention that high serum GOT activity observed in patients with myocardial infarction and acute heart failure is mostly attributed to the accompanying acute central necrosis of the liver assoc ...
... oxalacetic transaminase (GOT or AST) were elevated after acute myocardial infarction[2]. It is noteworthy to mention that high serum GOT activity observed in patients with myocardial infarction and acute heart failure is mostly attributed to the accompanying acute central necrosis of the liver assoc ...
Enzymes of the mevalonate pathway of isoprenoid
... C-HMG-CoA occurs slowly (0.3 per day) and that HMG-CoA is activated to form the E-S-HMG-CoA reaction intermediate. This activation proceeds through a tetrahedral carbon. Reversibility of this activation step accounts for incorporation into reisolated HMG-CoA of two 18O atoms at C5 when the reaction ...
... C-HMG-CoA occurs slowly (0.3 per day) and that HMG-CoA is activated to form the E-S-HMG-CoA reaction intermediate. This activation proceeds through a tetrahedral carbon. Reversibility of this activation step accounts for incorporation into reisolated HMG-CoA of two 18O atoms at C5 when the reaction ...
Lipid signaling
Lipid signaling, broadly defined, refers to any biological signaling event involving a lipid messenger that binds a protein target, such as a receptor, kinase or phosphatase, which in turn mediate the effects of these lipids on specific cellular responses. Lipid signaling is thought to be qualitatively different from other classical signaling paradigms (such as monoamine neurotransmission) because lipids can freely diffuse through membranes (see osmosis.) One consequence of this is that lipid messengers cannot be stored in vesicles prior to release and so are often biosynthesized ""on demand"" at their intended site of action. As such, many lipid signaling molecules cannot circulate freely in solution but, rather, exist bound to special carrier proteins in serum.