The Proteasomes
... structure, of proteasome and chaperone subunits are quite different. Secondly, the functions are different. Where proteasomes degrade proteins, chaperones provide a protective environment that facilitates proper folding. Of course, it is not surprising that the same conformational solution (the "iso ...
... structure, of proteasome and chaperone subunits are quite different. Secondly, the functions are different. Where proteasomes degrade proteins, chaperones provide a protective environment that facilitates proper folding. Of course, it is not surprising that the same conformational solution (the "iso ...
MODified™ Protein Domain Binding Kit Manual
... (PTMs) on histone tails is generated, interpreted and edited by proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. There are several classes of protein domains that influence gene regulation and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling ...
... (PTMs) on histone tails is generated, interpreted and edited by proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. There are several classes of protein domains that influence gene regulation and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling ...
Structural Basis for Bivalent Smac-Mimetics Recognition in the IAP
... ubiquitin ligase activity.9,10 The BIR domains host a zinc-finger motif and are generally composed of five α-helices and a three-stranded β-sheet. Some IAPs, like cIAP1 and cIAP2, contain also a caspaseassociated recruitment domain (CARD) located between the BIR3 domain and the C-terminal RING domai ...
... ubiquitin ligase activity.9,10 The BIR domains host a zinc-finger motif and are generally composed of five α-helices and a three-stranded β-sheet. Some IAPs, like cIAP1 and cIAP2, contain also a caspaseassociated recruitment domain (CARD) located between the BIR3 domain and the C-terminal RING domai ...
Increased MS Protein Identifi cation Rates Using 75 cm Long nano
... Peptide and Protein Identifications From a proteomics perspective, researchers in the field are commonly interested in the number of peptides identified, either in terms of peptide spectral matches, unique peptides or protein groups. As shown in Figure 4, the 75 cm column consistently results in the ...
... Peptide and Protein Identifications From a proteomics perspective, researchers in the field are commonly interested in the number of peptides identified, either in terms of peptide spectral matches, unique peptides or protein groups. As shown in Figure 4, the 75 cm column consistently results in the ...
Phosphorylation of Beclin 1 by DAP
... affects the interaction of Beclin 1 with Bcl-2. We first compared in silico the amino acids in Bcl-XL that form the binding pocket of T119 to the corresponding residues in Bcl-2 (Fig. 1). We found that these residues are conserved in Bcl-2, except for the solventexposed A104 in Bcl-XL, which is repl ...
... affects the interaction of Beclin 1 with Bcl-2. We first compared in silico the amino acids in Bcl-XL that form the binding pocket of T119 to the corresponding residues in Bcl-2 (Fig. 1). We found that these residues are conserved in Bcl-2, except for the solventexposed A104 in Bcl-XL, which is repl ...
The nutrient requirements of calves
... the diet. Although the essential role of amino acids is in forming proteins, they can be used as sources of energy when they are in excess to the calf’s protein requirements. In other words, if more protein is fed than required, it is used for energy in much the same way as the starch in cereal grai ...
... the diet. Although the essential role of amino acids is in forming proteins, they can be used as sources of energy when they are in excess to the calf’s protein requirements. In other words, if more protein is fed than required, it is used for energy in much the same way as the starch in cereal grai ...
Supplemental figure legends13092010HM
... Particles were initially centred against a rotationally average total sum and classified using multivariate statistical analysis (MSA) as implemented in IMAGIC-5 (van Heel et al, 1996). Classes were inspected and selected (based on the visual match between the class average and the individual parti ...
... Particles were initially centred against a rotationally average total sum and classified using multivariate statistical analysis (MSA) as implemented in IMAGIC-5 (van Heel et al, 1996). Classes were inspected and selected (based on the visual match between the class average and the individual parti ...
Modified Strains PJ69-7A and PJ69-7B for the Yeast Two
... The yeast two-hybrid system is a powerful technique used to detect protein-protein interactions. The technique has been used to establish physical interactions between genetically identified proteins, to identify the components of multiprotein complexes, and to map specific domains within proteins r ...
... The yeast two-hybrid system is a powerful technique used to detect protein-protein interactions. The technique has been used to establish physical interactions between genetically identified proteins, to identify the components of multiprotein complexes, and to map specific domains within proteins r ...
Data-driven docking for the study of biomolecular complexes
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
Solubility-enhancing proteins MBP and NusA play a passive role in
... equal number of cells producing MBP–GFP or NusA– GFP fusion proteins was compared. These two fusion proteins accumulate to comparable levels in E. coli (Fig. 2B). Whether in the presence or absence of IPTG, the Xuorescence intensities were very similar, indicating that there was no substantial diVer ...
... equal number of cells producing MBP–GFP or NusA– GFP fusion proteins was compared. These two fusion proteins accumulate to comparable levels in E. coli (Fig. 2B). Whether in the presence or absence of IPTG, the Xuorescence intensities were very similar, indicating that there was no substantial diVer ...
Hydrophilic Linkers and Polar Contacts Affect Aggregation of FG
... the third simulation, two separate aggregates formed initially, which subsequently merged into one. In all cases, the aggregates remained stable throughout the rest of the simulation. Next, we simulated the FSFG to SSSG mutation studied in the experiments by Frey et al. (30), which prevented hydroge ...
... the third simulation, two separate aggregates formed initially, which subsequently merged into one. In all cases, the aggregates remained stable throughout the rest of the simulation. Next, we simulated the FSFG to SSSG mutation studied in the experiments by Frey et al. (30), which prevented hydroge ...
Identification of Plasmodium falciparum var1CSA
... of Duffy binding-like (DBL) domains classified into six types (alpha, beta, gamma, delta, epsilon and type X) and cysteine-rich interdomain region domains (CIDR) classified into three types (alpha, beta and gamma) [10,11]. Var genes differ from one other by the number and the type of these domains. ...
... of Duffy binding-like (DBL) domains classified into six types (alpha, beta, gamma, delta, epsilon and type X) and cysteine-rich interdomain region domains (CIDR) classified into three types (alpha, beta and gamma) [10,11]. Var genes differ from one other by the number and the type of these domains. ...
uos--smith_20060314
... We agree that axioms and formal definitions are an indispensable part of creating semantic interoperability catalyzed through an upper ontology But an upper ontology will only be used by domain scientists if we provide humanly intelligible equivalents to these axioms and formal definitions These equ ...
... We agree that axioms and formal definitions are an indispensable part of creating semantic interoperability catalyzed through an upper ontology But an upper ontology will only be used by domain scientists if we provide humanly intelligible equivalents to these axioms and formal definitions These equ ...
F-actin Sequesters Elongation Factor from Interaction with
... with cAMP induces cytoplasmic alkalinization, and artificially raising the intracellular pH can trigger a severalfold increase in the rate of DNA and protein synthesis (Aerts et al., 1985, 1987). The interaction of Dictyostelium EF-Iot with F-actin is pH-dependent with a transition from tight to loo ...
... with cAMP induces cytoplasmic alkalinization, and artificially raising the intracellular pH can trigger a severalfold increase in the rate of DNA and protein synthesis (Aerts et al., 1985, 1987). The interaction of Dictyostelium EF-Iot with F-actin is pH-dependent with a transition from tight to loo ...
Gamma globulins
... different heavy chain (γ, α, µ, δ, or ε). • Disulfide bonds are indicated by green lines. The IgA dimer and the IgM pentamer have a small polypeptide chain in addition to the light and heavy chains. Dr Gihan Gawish ...
... different heavy chain (γ, α, µ, δ, or ε). • Disulfide bonds are indicated by green lines. The IgA dimer and the IgM pentamer have a small polypeptide chain in addition to the light and heavy chains. Dr Gihan Gawish ...
Scaffold nucleoporins Nup188 and Nup192 share
... To determine the structure of Nup188 we first expressed full-length protein from Saccharomyces cerevisiae (Sc) and the thermophilic fungus Myceliophthora thermophila (Mt) (Berka et al., 2011). Although the Mt homolog behaved better in vitro, it did not yield crystals of the full-length protein. Usin ...
... To determine the structure of Nup188 we first expressed full-length protein from Saccharomyces cerevisiae (Sc) and the thermophilic fungus Myceliophthora thermophila (Mt) (Berka et al., 2011). Although the Mt homolog behaved better in vitro, it did not yield crystals of the full-length protein. Usin ...
Orthologs, paralogs and homology inference Where are we now?
... – identify genes in reference genomes that may have the same or similar functions, so that comprehensive curation can be done simultaneously ...
... – identify genes in reference genomes that may have the same or similar functions, so that comprehensive curation can be done simultaneously ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.