Download Modified Strains PJ69-7A and PJ69-7B for the Yeast Two

Modified Strains PJ69-7A and PJ69-7B for
the Yeast Two-Hybrid System
INVENTORS • Philip James
WARF: P00227US
Assigned to WARF as biological material.
Since its founding in 1925 as the patenting and
licensing organization for the University of Wisconsin-
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial
partners interested in developing two new strains for the yeast two-hybrid
system.
Madison, WARF has been working with business and
industry to transform university research into products
that benefit society. WARF intellectual property
managers and licensing staff members are leaders in
the field of university-based technology transfer. They
OVERVIEW
are familiar with the intricacies of patenting, have
worked with researchers in relevant disciplines,
understand industries and markets, and have
The yeast two-hybrid system is a powerful technique used to detect protein-protein
interactions. The technique has been used to establish physical interactions between
genetically identified proteins, to identify the components of multiprotein complexes, and
to map specific domains within proteins responsible for an interaction. However, there are
several limitations to the two-hybrid system, including host strains that result in a large
number of false positives.
negotiated innovative licensing strategies to meet the
individual needs of business clients.
THE INVENTION
A UW-Madison researcher has developed two strains, known as PJ69-7A and PJ69-7B,
that eliminate many of the problems associated with false positives. In addition, the
strains include both mating types, allowing high throughput screening.
APPLICATIONS
• Yeast two-hybrid system for identifying interactions between proteins
KEY BENEFITS
• The strains incorporate three different markers all with different promoters.
• The strains contain a non-leaky HIS3 marker that eliminates the use of high
concentrations of the drug 3-aminotriazole (3-aminotriazole inhibits desired positive
clones), improving sensitivity.
• The strains improve efficiency of clone selection and the ease of false positive
screening, quickly and easily screening out nearly all false positives with simple plate
assays.
• The decrease in false positives will reduce the number of liquid assays required to
Wisconsin Alumni Research Foundation | 614 Walnut Street, 13th Floor | Madison, WI 53726 | [email protected] | www.warf.org
WARF: P00227US
identify proteins that have an interaction.
• In addition, plasmid-loss tests are made faster and simpler by using counter-selectable URA3 and ADE2 markers.
ADDITIONAL INFORMATION
Tech Fields
Research Tools - Protein interactions & function
CONTACT INFORMATION
For current licensing status, please contact Jennifer Gottwald at [email protected] or (608) 262-5941.
Wisconsin Alumni Research Foundation | 614 Walnut Street, 13th Floor | Madison, WI 53726 | [email protected] | www.warf.org