Identification and Cloning of a Cryptococcal Deacetylase That
... sharing thè ability to bind to concanavalin A (ConA) columns. The structure and antigenic properties of individuai MP components have been only partially characterized (4,5). A 98-kDa MP, with homology to chitin deacetylases, was recently cloned and shown to stimolate a mouse CD4+-T-cell hybridoma t ...
... sharing thè ability to bind to concanavalin A (ConA) columns. The structure and antigenic properties of individuai MP components have been only partially characterized (4,5). A 98-kDa MP, with homology to chitin deacetylases, was recently cloned and shown to stimolate a mouse CD4+-T-cell hybridoma t ...
{PDOC00000} {BEGIN
... protein plays an important role in the regulation of N-glycosylation [2]. It has been shown [3] that the presence of proline between Asn and Ser/Thr will inhibit N-glycosylation; this has been confirmed by a recent [4] statistical analysis of glycosylation sites, which also shows that about 50% of t ...
... protein plays an important role in the regulation of N-glycosylation [2]. It has been shown [3] that the presence of proline between Asn and Ser/Thr will inhibit N-glycosylation; this has been confirmed by a recent [4] statistical analysis of glycosylation sites, which also shows that about 50% of t ...
PDF - Beilstein
... peptide NisA showed that dehydration involves the glutamylation of Ser and Thr side chains prior to elimination of glutamate [50]. This mechanistic proposal was established due to the observation that three NisB mutants (R786A, R826A and H961A) were able to transfer multiple glutamates to NisA witho ...
... peptide NisA showed that dehydration involves the glutamylation of Ser and Thr side chains prior to elimination of glutamate [50]. This mechanistic proposal was established due to the observation that three NisB mutants (R786A, R826A and H961A) were able to transfer multiple glutamates to NisA witho ...
CHAPTER 1 INTRODUCTION
... With the rising costs of energy, and limited availability of land greater emphasis on crop agriculture seems inevitable. In the future, plant proteins must provide an even greater proportion of our food protein. Cereals , as they traditionally have, may supply most of this, however , soya bean and, ...
... With the rising costs of energy, and limited availability of land greater emphasis on crop agriculture seems inevitable. In the future, plant proteins must provide an even greater proportion of our food protein. Cereals , as they traditionally have, may supply most of this, however , soya bean and, ...
MDM2 inhibit p300-mediated p53 acetylation and activation by
... MDM2 can inhibit p53 acetylation by p300 probably through direct association. ...
... MDM2 can inhibit p53 acetylation by p300 probably through direct association. ...
Identification and characterization of novel interaction
... Phosphoproteine HPrSer46P und CrhP sowie die niedermolekularen Effektoren Fruktose-1,6bisphosphat (FBP) und Glukose-6-Phosphat (Glc-6-P) stimulieren die DNA-Bindefähigkeit von CcpA in Gegenwart einer bevorzugten Kohlenstoffquelle, wie beispielsweise Glukose. Die Bindung dieser Phosphoproteine an Ccp ...
... Phosphoproteine HPrSer46P und CrhP sowie die niedermolekularen Effektoren Fruktose-1,6bisphosphat (FBP) und Glukose-6-Phosphat (Glc-6-P) stimulieren die DNA-Bindefähigkeit von CcpA in Gegenwart einer bevorzugten Kohlenstoffquelle, wie beispielsweise Glukose. Die Bindung dieser Phosphoproteine an Ccp ...
Functional characterization of rare FOXP2 variants in
... variants, but their phenotypic relevance is often unclear. FOXP2 encodes a transcription factor with a forkhead box DNA-binding domain, but little is known about the functions of protein regions outside this domain. Methods: We performed detailed functional analyses of seven rare FOXP2 variants foun ...
... variants, but their phenotypic relevance is often unclear. FOXP2 encodes a transcription factor with a forkhead box DNA-binding domain, but little is known about the functions of protein regions outside this domain. Methods: We performed detailed functional analyses of seven rare FOXP2 variants foun ...
Transcription factor Sp3 is silenced through - IMT
... Sp3, however, appears to be different since Sp3- and Sp1de®cient mice exhibit different phenotypes (Marin et al., 1997; Bouwman et al., 2000). Cell culture experiments also revealed signi®cant differences between Sp1 and Sp3. On many reporter constructs containing multiple Spbinding sites, Sp3, unli ...
... Sp3, however, appears to be different since Sp3- and Sp1de®cient mice exhibit different phenotypes (Marin et al., 1997; Bouwman et al., 2000). Cell culture experiments also revealed signi®cant differences between Sp1 and Sp3. On many reporter constructs containing multiple Spbinding sites, Sp3, unli ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... composed of 120 amino acids with an approximate molecular mass of 12 kDa. L12 proteins form strong dimers not only on the ribosome but also in solution. The dimerization involves the N-terminal domains (NTDs) composed of residues 1–37. In a L12 monomer, this domain is folded into two a-helices arran ...
... composed of 120 amino acids with an approximate molecular mass of 12 kDa. L12 proteins form strong dimers not only on the ribosome but also in solution. The dimerization involves the N-terminal domains (NTDs) composed of residues 1–37. In a L12 monomer, this domain is folded into two a-helices arran ...
METABOLIC CUES AND REGULATORY PROTEINS
... establish an intracellular niche protected from digestion. Moreover, if humans inhale bacteria-laden aerosols, L. pneumophila can survive and replicate within alveolar macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates ...
... establish an intracellular niche protected from digestion. Moreover, if humans inhale bacteria-laden aerosols, L. pneumophila can survive and replicate within alveolar macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
PDF Full-text
... a Ni complex on the surface of a substrate. An advantage of this method is that all proteins coordinate in the same direction, which prevents deactivation. It has been reported that 80% of 5800 kinds of yeast proteins maintained their activity when immobilized using this method. Katayama et al. also ...
... a Ni complex on the surface of a substrate. An advantage of this method is that all proteins coordinate in the same direction, which prevents deactivation. It has been reported that 80% of 5800 kinds of yeast proteins maintained their activity when immobilized using this method. Katayama et al. also ...
ldentification of Surface-Exposed Domains on the Reducing Side of
... shown). PsaA-PsaB remained largely intact at Glu-C concentrations up to 20 ng/mg Chl. At higher protease concentrations, more fragments were observed; most of these were detected only after silver staining. PsaL, PsaK, Psa], Psal, and PsaM were resistant to Glu-C cleavage at all protease concentrati ...
... shown). PsaA-PsaB remained largely intact at Glu-C concentrations up to 20 ng/mg Chl. At higher protease concentrations, more fragments were observed; most of these were detected only after silver staining. PsaL, PsaK, Psa], Psal, and PsaM were resistant to Glu-C cleavage at all protease concentrati ...
Forced Expression of Dystrophin Deletion Constructs Reveals
... and is thought to be present in most dystrophin complexes (36). pl-syntrophin is present at low levels in skeletal muscle, but its precise localization pattern has not been reported (1). 132-syntrophin colocalizes at the neuromuscular junction with utrophin, and is hypothesized to associate in compl ...
... and is thought to be present in most dystrophin complexes (36). pl-syntrophin is present at low levels in skeletal muscle, but its precise localization pattern has not been reported (1). 132-syntrophin colocalizes at the neuromuscular junction with utrophin, and is hypothesized to associate in compl ...
Bacterial protein toxins targeting Rho GTPases
... GTPases (e.g. Cdc42 and Rac) [10]. C3 exoenzymes ADPribosylate Rho at Asn41 [11] thereby blocking Rho-mediated signalling. Although the target amino acid residue is located near the e¡ector region of Rho, ADP-ribosylation does not block the Rho/e¡ector interaction [12] nor are major e¡ects on guanin ...
... GTPases (e.g. Cdc42 and Rac) [10]. C3 exoenzymes ADPribosylate Rho at Asn41 [11] thereby blocking Rho-mediated signalling. Although the target amino acid residue is located near the e¡ector region of Rho, ADP-ribosylation does not block the Rho/e¡ector interaction [12] nor are major e¡ects on guanin ...
Purification of Arrestin from Bovine Retinas
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
- Free Documents
... into proinsulin. Proinsulin is Iurther cleaved yielding active insulin which is composed oI two peptide chains linked togehter through disulIide bonds. Still other proteins oI the enzyme class are synthesized as inactive precursors called zymogens. Zymogens are activated by proteolytic cleavage such ...
... into proinsulin. Proinsulin is Iurther cleaved yielding active insulin which is composed oI two peptide chains linked togehter through disulIide bonds. Still other proteins oI the enzyme class are synthesized as inactive precursors called zymogens. Zymogens are activated by proteolytic cleavage such ...
Barley Aleurone Cells Contain Two Types of
... Figure 1 shows that barley aleurone cells contain protein storage vacuoles and a distinctly different kind of organelle, which we have called secondary vacuoles. Differential interference contrast (DIC) microscopy showed that these two types of vacuole occur in both GA- and ABA-treated aleurone prot ...
... Figure 1 shows that barley aleurone cells contain protein storage vacuoles and a distinctly different kind of organelle, which we have called secondary vacuoles. Differential interference contrast (DIC) microscopy showed that these two types of vacuole occur in both GA- and ABA-treated aleurone prot ...
From Sequence to Structure
... signaling molecule. This function exploits the ability of proteins to present structurally and chemically diverse surfaces that can interact with other molecules with high specificity. Catalysis requires not only specific binding, to substrates and in some cases to regulatory molecules, but also spe ...
... signaling molecule. This function exploits the ability of proteins to present structurally and chemically diverse surfaces that can interact with other molecules with high specificity. Catalysis requires not only specific binding, to substrates and in some cases to regulatory molecules, but also spe ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
... binding the outer PC -hexam ers to the basal AP-disc. A lternatively, one o f the presum ed rodcore linkers w ould have to play this role, being in reality a m isnam ed rod linker. In this context it is interesting th a t an (a ß )6PC • L r c 2 9 5 •(a ß )3AP • L c 8 9 com plex o f unknow n m o rpho ...
... binding the outer PC -hexam ers to the basal AP-disc. A lternatively, one o f the presum ed rodcore linkers w ould have to play this role, being in reality a m isnam ed rod linker. In this context it is interesting th a t an (a ß )6PC • L r c 2 9 5 •(a ß )3AP • L c 8 9 com plex o f unknow n m o rpho ...
General principles of cellular organization in the genome
... latter show decaying expression in a staircase-like manner. Under various conditions, operons General principles of cellular organization in Mycoplasma pneumoniae ...
... latter show decaying expression in a staircase-like manner. Under various conditions, operons General principles of cellular organization in Mycoplasma pneumoniae ...
and Rhizobiales-Like PPP-Family Protein Phosphatases from
... biosynthesis and mitochondrial intermembrane space processes, respectively. Lastly, phenotypic analysis of atslp2 insertional mutant and 35S::AtSLP2 over-expression plants revealed biological involvement in regulating gibberellic acid-related processes during seed germination as well as ...
... biosynthesis and mitochondrial intermembrane space processes, respectively. Lastly, phenotypic analysis of atslp2 insertional mutant and 35S::AtSLP2 over-expression plants revealed biological involvement in regulating gibberellic acid-related processes during seed germination as well as ...
Protein Creation Pathway
... organelles. Remember, they are created by the nucleolus. After exiting the nucleus they themselves are responsible for the production of proteins within the cell. Because they are so small, they resembles tiny dots in this picture. After they exit the nucleus, the ribosomes are often attached to the ...
... organelles. Remember, they are created by the nucleolus. After exiting the nucleus they themselves are responsible for the production of proteins within the cell. Because they are so small, they resembles tiny dots in this picture. After they exit the nucleus, the ribosomes are often attached to the ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.