SCJ4600048 - Sigma
... Dissolve 5 mg of the antibody in ∼2 mL of 0.1 M sodium bicarbonate buffer, pH ∼8.3, resulting in the Labeling Solution. If the IgG has been previously dissolved in phosphate buffer, such as PBS buffer (must be free of any amine-containing chemicals), the Labeling Solution can be conveniently prepare ...
... Dissolve 5 mg of the antibody in ∼2 mL of 0.1 M sodium bicarbonate buffer, pH ∼8.3, resulting in the Labeling Solution. If the IgG has been previously dissolved in phosphate buffer, such as PBS buffer (must be free of any amine-containing chemicals), the Labeling Solution can be conveniently prepare ...
STING Millennium: a web-based suite of programs
... because any point on the structure/surface might be painted (colored) only once, following the color code designed for given amino acid characteristics. Amino acid sequence on the other hand, can be mapped by number of parameters, organized and displayed in underlying rows. Appropriate visualization ...
... because any point on the structure/surface might be painted (colored) only once, following the color code designed for given amino acid characteristics. Amino acid sequence on the other hand, can be mapped by number of parameters, organized and displayed in underlying rows. Appropriate visualization ...
Introduction to Database Searching using MASCOT
... induced to fragment, possibly by collision, and the second stage of mass analysis used to collect an MS/MS spectrum. Because we are collecting data from isolated peptides, it makes no difference whether the original sample was a mixture or not. We identify peptide sequences, and then try to assign t ...
... induced to fragment, possibly by collision, and the second stage of mass analysis used to collect an MS/MS spectrum. Because we are collecting data from isolated peptides, it makes no difference whether the original sample was a mixture or not. We identify peptide sequences, and then try to assign t ...
Branched-Chain Amino Acid Supplementation: A Critical Analysis of
... Branched-chain amino acids (BCAAs), the name referring to the unique branching structure of their residual side chain. BCAAs play a crucial role in many biochemical processes essential to human life. They represent three of nine amino acids that cannot be synthesized in the human body. For this reas ...
... Branched-chain amino acids (BCAAs), the name referring to the unique branching structure of their residual side chain. BCAAs play a crucial role in many biochemical processes essential to human life. They represent three of nine amino acids that cannot be synthesized in the human body. For this reas ...
Regulation of phospholipase D activity, membrane targeting and
... a radioactive form of one of these derivatives was used to label the protein in a manner that could be protected by an excess of PtdIns(4,5)P2 but not by other phosphoinositides or other acidic lipids that do not activate the enzyme [16]. Taken together, these results strongly suggested that a bindi ...
... a radioactive form of one of these derivatives was used to label the protein in a manner that could be protected by an excess of PtdIns(4,5)P2 but not by other phosphoinositides or other acidic lipids that do not activate the enzyme [16]. Taken together, these results strongly suggested that a bindi ...
novel therapy of high-priority citrus diseases
... mechanisms of disease development by these three gramnegative bacteria are different2, 4, it is our hypothesis that there are common and conserved features that can be targeted through a common therapy. In this project, the protein chimera or fusion proteins are created in such a way that they have ...
... mechanisms of disease development by these three gramnegative bacteria are different2, 4, it is our hypothesis that there are common and conserved features that can be targeted through a common therapy. In this project, the protein chimera or fusion proteins are created in such a way that they have ...
PRIONS THE INFECTIOUS PROTEINS
... Factors that prevent Prion Replication Phospholipase A2 Inhibitors prevent prion replication. Platelet-activating Factor Antagonists also inhibits prion ...
... Factors that prevent Prion Replication Phospholipase A2 Inhibitors prevent prion replication. Platelet-activating Factor Antagonists also inhibits prion ...
Reprint
... results in the loss of its eRF3-binding properties both in vivo and in vitro (Fig. 1). Such deletions alter a conserved GFGGIGxxxRY motif present at the C-terminus of eRF1 [18] which suggests that it might play a key role in eRF1 binding to eRF3. Also, the high number of acidic residues present in t ...
... results in the loss of its eRF3-binding properties both in vivo and in vitro (Fig. 1). Such deletions alter a conserved GFGGIGxxxRY motif present at the C-terminus of eRF1 [18] which suggests that it might play a key role in eRF1 binding to eRF3. Also, the high number of acidic residues present in t ...
Relationship between Hot Spot Residues and Ligand Binding Hot
... alanine scanning mutagenesis. In contrast, in pharmaceutical research, a hot spot is a site on a target protein that has high propensity for ligand binding and hence is potentially important for drug discovery. Here we examine the relationship between these two hot spot concepts by comparing alanine ...
... alanine scanning mutagenesis. In contrast, in pharmaceutical research, a hot spot is a site on a target protein that has high propensity for ligand binding and hence is potentially important for drug discovery. Here we examine the relationship between these two hot spot concepts by comparing alanine ...
2006 Mega Molecules, LLC
... Consuming enough protein is vital for maintaining health. Proteins do all of the work in the body – they help form important structures in the body, regulate many body functions (increase or decrease cellular activity based on signals within the body), provide communication throughout the body, and ...
... Consuming enough protein is vital for maintaining health. Proteins do all of the work in the body – they help form important structures in the body, regulate many body functions (increase or decrease cellular activity based on signals within the body), provide communication throughout the body, and ...
supplemental materials
... reconstruction of the vacant E. coli 70S ribosome (Supp. Fig. 1, grey box on top row, depicted in both side and top view) as a new reference to the already refined reconstructions. Iterative refinement in one sorting step was continued until stable angular assignment and subpopulations were obtained ...
... reconstruction of the vacant E. coli 70S ribosome (Supp. Fig. 1, grey box on top row, depicted in both side and top view) as a new reference to the already refined reconstructions. Iterative refinement in one sorting step was continued until stable angular assignment and subpopulations were obtained ...
Protein Arginine Methylation in Candida albicans: Role
... C. albicans HMT1 gene and surrounding regions were cloned by PCR amplification of BWP17 genomic DNA with oligonucleotides AM113 and AM114, XhoI and SpeI digestion, and insertion into XhoI-SpeI-digested pRS315, resulting in pAM322. The C. albicans HMT1 reconstitution plasmid (pAM385) resulted from th ...
... C. albicans HMT1 gene and surrounding regions were cloned by PCR amplification of BWP17 genomic DNA with oligonucleotides AM113 and AM114, XhoI and SpeI digestion, and insertion into XhoI-SpeI-digested pRS315, resulting in pAM322. The C. albicans HMT1 reconstitution plasmid (pAM385) resulted from th ...
Structural and Functional Comparisons of Retroviral Envelope
... view of gp41 (and, thus, Env as a whole) as a type I membrane protein, with an extracellular N-terminus, a single MSD and an approximately 150 amino acid-long cytoplasmic C-terminal tail (CTT) [2]. More recent studies, however, indicate that the CTT topology may be more dynamic and complex than prev ...
... view of gp41 (and, thus, Env as a whole) as a type I membrane protein, with an extracellular N-terminus, a single MSD and an approximately 150 amino acid-long cytoplasmic C-terminal tail (CTT) [2]. More recent studies, however, indicate that the CTT topology may be more dynamic and complex than prev ...
Document
... Once protein-protein interactions have been identified, it is important to establish how the interactions occur e.g. what regions or specific amino acids are important for the interaction? Well-used approach is to prepare different fragments or mutations of proteins and see if there is any effec ...
... Once protein-protein interactions have been identified, it is important to establish how the interactions occur e.g. what regions or specific amino acids are important for the interaction? Well-used approach is to prepare different fragments or mutations of proteins and see if there is any effec ...
Vertebrate hairy and Enhancer of split related proteins
... hairy-like and E(spl)-like proteins have been named HES (hairy and enhancer of split) or her (hairy and enhancer of split related) in mammals and zebra®sh respectively, and numbered by the order of isolation. Hey proteins have also been named HRT or Hesr, or in the case of Hey2, Gridlock or CHF1, wh ...
... hairy-like and E(spl)-like proteins have been named HES (hairy and enhancer of split) or her (hairy and enhancer of split related) in mammals and zebra®sh respectively, and numbered by the order of isolation. Hey proteins have also been named HRT or Hesr, or in the case of Hey2, Gridlock or CHF1, wh ...
The presence of monoglucosylated N196
... It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays critical roles during a variety of cellular processes, such as protein folding, protein–protein interaction, immune response and responses to pathogens [2–4]. More than half of all eukar ...
... It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays critical roles during a variety of cellular processes, such as protein folding, protein–protein interaction, immune response and responses to pathogens [2–4]. More than half of all eukar ...
Bluetongue virus: dissection of the polymerase complex
... have been made in recent years in understanding the replicase complexes of these viruses, including BTV. Each of the BTV proteins that form the complex has been expressed as a recombinant protein, purified and used to develop an in vitro assay system for activity. This, in turn, has led to the detai ...
... have been made in recent years in understanding the replicase complexes of these viruses, including BTV. Each of the BTV proteins that form the complex has been expressed as a recombinant protein, purified and used to develop an in vitro assay system for activity. This, in turn, has led to the detai ...
Poster
... cell migration. Mice with mutations in genes encoding ADAM17, EGFR, and EGFR ligands exhibit defects in embryonic eyelid closure. Recently, woe (wavy with open eyelids) mice, also exhibiting defects in embryonic eyelid closure, were identified. Genetic analysis of woe mice identified a mutation in A ...
... cell migration. Mice with mutations in genes encoding ADAM17, EGFR, and EGFR ligands exhibit defects in embryonic eyelid closure. Recently, woe (wavy with open eyelids) mice, also exhibiting defects in embryonic eyelid closure, were identified. Genetic analysis of woe mice identified a mutation in A ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.