Poster
... neurotransmitter, acetylcholine, from motor neurons associated with skeletal muscle. Despite this toxicity, BoNT/A is used pharmaceutically as a treatment for numerous neurological diseases, such as migraines and dystonias, and as an anti-wrinkle agent in cosmetic surgery. BoNT/A is one of seven ser ...
... neurotransmitter, acetylcholine, from motor neurons associated with skeletal muscle. Despite this toxicity, BoNT/A is used pharmaceutically as a treatment for numerous neurological diseases, such as migraines and dystonias, and as an anti-wrinkle agent in cosmetic surgery. BoNT/A is one of seven ser ...
Two Separate Functions Are Encoded by the Carboxyl
... (11–15). Comparative analysis reveals that these proteins are structurally similar and bear the highest degree of homology in regions comprising the carboxyl terminus and a proline-rich region located between the functional domains. In all CAP proteins and their homologs, the latter region contains ...
... (11–15). Comparative analysis reveals that these proteins are structurally similar and bear the highest degree of homology in regions comprising the carboxyl terminus and a proline-rich region located between the functional domains. In all CAP proteins and their homologs, the latter region contains ...
Molecular architecture of the glomerular slit
... protein. In autosomal dominant familial focal segmental glomerulosclerosis, a-actinin-4 is typically mutated [38], suggesting that a-actinin-4 is a robust linker molecule, mediating and modulating interactions of the slit diaphragm domain molecules with the actin cytoskeleton of podocytes. Densin is ...
... protein. In autosomal dominant familial focal segmental glomerulosclerosis, a-actinin-4 is typically mutated [38], suggesting that a-actinin-4 is a robust linker molecule, mediating and modulating interactions of the slit diaphragm domain molecules with the actin cytoskeleton of podocytes. Densin is ...
tr-kit promotes the formation of a multimolecular complex composed
... carboxyl-terminal domain and it is the main substrate for Src-like kinases in mitosis (Taylor and Shalloway, 1994); it has been demonstrated that the interaction with the SH3 domain of Fyn is necessary for Fyn-dependent tyrosine phosphorylation of Sam68 (Shen et al., 1999). Once phosphorylated in th ...
... carboxyl-terminal domain and it is the main substrate for Src-like kinases in mitosis (Taylor and Shalloway, 1994); it has been demonstrated that the interaction with the SH3 domain of Fyn is necessary for Fyn-dependent tyrosine phosphorylation of Sam68 (Shen et al., 1999). Once phosphorylated in th ...
Molecular assembly of the period-cryptochrome circadian
... domain and an α-helical domain (Figure 1C). Consistent with their high sequence homology (86%), the crystal structures of CRY1-PHR and CRY2-PHR can be superimposed with a root-mean-square deviation (RMSD) of 0.43 Å out of 377 aligned Cα atoms. To gain structural insights into the general interaction ...
... domain and an α-helical domain (Figure 1C). Consistent with their high sequence homology (86%), the crystal structures of CRY1-PHR and CRY2-PHR can be superimposed with a root-mean-square deviation (RMSD) of 0.43 Å out of 377 aligned Cα atoms. To gain structural insights into the general interaction ...
Allosteric Modulators of Steroid Hormone Receptors: Structural
... importance of residues outside the ligand binding pocket that contributed to receptor-specific ligand interactions and allosteric communication with the coactivator binding surface (64). In particular, the position of helix 11 was implicated in ER subtype-selective agonist, partial agonist, or antag ...
... importance of residues outside the ligand binding pocket that contributed to receptor-specific ligand interactions and allosteric communication with the coactivator binding surface (64). In particular, the position of helix 11 was implicated in ER subtype-selective agonist, partial agonist, or antag ...
Structure and mechanism of ATP-dependent phospholipid transporters
... from the cytoplasm, and two distinct “importer” folds, typical of proteins that transport substrates toward the cytoplasm. The typical ABC “exporter” fold contains a total of 12 transmembrane spanning α-helices that form the substrate binding site and provide the pathway for the movement of substanc ...
... from the cytoplasm, and two distinct “importer” folds, typical of proteins that transport substrates toward the cytoplasm. The typical ABC “exporter” fold contains a total of 12 transmembrane spanning α-helices that form the substrate binding site and provide the pathway for the movement of substanc ...
The Structure of Human Prions: From Biology to Structural Models
... occurred within the N-terminal ectodomain of a ZIP predecessor gene, when metazoans first emerged on the planet. As a consequence of this rearrangement a cysteine-flanked core (CFC) region was generated within this ectodomain. The second event, which allowed the creation of the prion protein founder ...
... occurred within the N-terminal ectodomain of a ZIP predecessor gene, when metazoans first emerged on the planet. As a consequence of this rearrangement a cysteine-flanked core (CFC) region was generated within this ectodomain. The second event, which allowed the creation of the prion protein founder ...
Integrin cytoplasmic domain-binding proteins
... have Tyr→Phe mutations in the β3 tail are less efficient at clot retraction, and these mutations are associated with a mild bleeding phenotype in mice (Jenkins et al., 1998; Law et al., 1999). In addition, Sajid et al. recently showed that non-muscle myosin heavy chain A coimmunoprecipitates with αV ...
... have Tyr→Phe mutations in the β3 tail are less efficient at clot retraction, and these mutations are associated with a mild bleeding phenotype in mice (Jenkins et al., 1998; Law et al., 1999). In addition, Sajid et al. recently showed that non-muscle myosin heavy chain A coimmunoprecipitates with αV ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... important for maintaining the integrity of the Golgi apparatus has been identified in animals and yeast (Figure 2). Golgins are large proteins with extensive coiled-coil domains [20–22]. The coiled-coil is a very common protein motif that consists of heptad repeats, which form amphipatic a-helices t ...
... important for maintaining the integrity of the Golgi apparatus has been identified in animals and yeast (Figure 2). Golgins are large proteins with extensive coiled-coil domains [20–22]. The coiled-coil is a very common protein motif that consists of heptad repeats, which form amphipatic a-helices t ...
Structure based hypothesis of a mitochondrial
... mtRF1a. Furthermore, insertion of two amino acids (RT) prior to Thr-115 results in a distinctly altered conformation of the loop containing Gly-116. The threonine of the RT-insert (T-RTi) points inwards into the RF1 nucleotide binding pocket, creating a hydrogen bonding interaction to the backbone o ...
... mtRF1a. Furthermore, insertion of two amino acids (RT) prior to Thr-115 results in a distinctly altered conformation of the loop containing Gly-116. The threonine of the RT-insert (T-RTi) points inwards into the RF1 nucleotide binding pocket, creating a hydrogen bonding interaction to the backbone o ...
Prediction of protease substrates using sequence
... an unclassified vector as falling on one side or the other of the separation. Each dimension in the vector is a feature number, which has a corresponding value. Here, a single cleavage sequence had eight features representing its oligopeptide sequence. Each residue was assigned a feature number by t ...
... an unclassified vector as falling on one side or the other of the separation. Each dimension in the vector is a feature number, which has a corresponding value. Here, a single cleavage sequence had eight features representing its oligopeptide sequence. Each residue was assigned a feature number by t ...
proposal-aug25
... knowledge, this analysis will represent a totally novel approach to identify (and quantify) the functional content of disordered regions. Preliminary results: Applying our phyloHMM to alignments of the entire yeast proteome yields >7000 short conserved segments, of which we expect only ~ 200 to be f ...
... knowledge, this analysis will represent a totally novel approach to identify (and quantify) the functional content of disordered regions. Preliminary results: Applying our phyloHMM to alignments of the entire yeast proteome yields >7000 short conserved segments, of which we expect only ~ 200 to be f ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... groundwork for the development of new therapeutic strategies targeting multiple chaperones and cochaperones. Published by Elsevier Ltd. ...
... groundwork for the development of new therapeutic strategies targeting multiple chaperones and cochaperones. Published by Elsevier Ltd. ...
Reivew, Hemoglobin
... Blood transports two forms of CO2 to the lungs: carbamino-hemoglobin and H2CO3/HCO3- (carbonic acidconjugate base pair) 1. Carbamino-hemoglobin: exposure to low pCO2 results in the reversal of the carbamination reaction by mass action and O2 binding is again favored. CO2 is expelled by the lungs. ...
... Blood transports two forms of CO2 to the lungs: carbamino-hemoglobin and H2CO3/HCO3- (carbonic acidconjugate base pair) 1. Carbamino-hemoglobin: exposure to low pCO2 results in the reversal of the carbamination reaction by mass action and O2 binding is again favored. CO2 is expelled by the lungs. ...
Effects of Macromolecular Crowding on Protein Folding
... tration but independent of dextran size (for dextrans ranging from 20 to 70 kDa). A theoretical crowding model was used to investigate the origins of this stabilization. In this model, Dextran and Ficoll were modeled as elongated rods and the protein was represented as a sphere, with the folded sph ...
... tration but independent of dextran size (for dextrans ranging from 20 to 70 kDa). A theoretical crowding model was used to investigate the origins of this stabilization. In this model, Dextran and Ficoll were modeled as elongated rods and the protein was represented as a sphere, with the folded sph ...
Fulllength archaeal Rad51 structure and
... Ala100 (b0) contacting Ile200 and Val202 (b3), which extends the central b-sheet through main-chain hydrogen bonds (Figures 1A±C, and 2D and G). Notably, an analogous intermolecular b-sheet is seen in the helical EcRecA X-ray crystal structure by b0 contacting b3 (Story et al., 1992). Therefore the ...
... Ala100 (b0) contacting Ile200 and Val202 (b3), which extends the central b-sheet through main-chain hydrogen bonds (Figures 1A±C, and 2D and G). Notably, an analogous intermolecular b-sheet is seen in the helical EcRecA X-ray crystal structure by b0 contacting b3 (Story et al., 1992). Therefore the ...
A new look at sodium channel b subunits
... membrane resting potential of about 270 mV (the membrane potential is defined relative to the extracellular medium; a potential of 270 mV implies that the cell interior is negative relative to the exterior). The negative resting membrane potential is largely set by the greater membrane permeability ...
... membrane resting potential of about 270 mV (the membrane potential is defined relative to the extracellular medium; a potential of 270 mV implies that the cell interior is negative relative to the exterior). The negative resting membrane potential is largely set by the greater membrane permeability ...
Functional Roles Of Lipids In membranes - IJS
... disrupt the stable hydrogen bonded structure of water and therefore are at an energy minimum when such domains self associate to minimize the total surface area in contact with water. The polar domains of lipids interact either through hydrogen bonding or ...
... disrupt the stable hydrogen bonded structure of water and therefore are at an energy minimum when such domains self associate to minimize the total surface area in contact with water. The polar domains of lipids interact either through hydrogen bonding or ...
Conserved mechanisms of Ras regulation of evolutionary
... activated Ras and Ets1 p68 deletion mutants. The data from three duplicate experiments were averaged and the error bars indicate the standard deviation. The fold activation of the reporter in presence of the empty ets expression vector (0) is set to 1. The features of the Ets1 p68 protein are illust ...
... activated Ras and Ets1 p68 deletion mutants. The data from three duplicate experiments were averaged and the error bars indicate the standard deviation. The fold activation of the reporter in presence of the empty ets expression vector (0) is set to 1. The features of the Ets1 p68 protein are illust ...
MICROSTRUCTURE OF PROTEIN BODIES IN MARAMA BEAN SPECIES
... location of protein bodies relative to fat has been found to be similar to that of soya bean. These finding suggest that the extraction of marama protein may be done in the same way as soya. The presence of crystalline inclusion in marama bean, suggests that phytic acid in marama may be predominantl ...
... location of protein bodies relative to fat has been found to be similar to that of soya bean. These finding suggest that the extraction of marama protein may be done in the same way as soya. The presence of crystalline inclusion in marama bean, suggests that phytic acid in marama may be predominantl ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.