serine proteases
... 2. New +NH3 turns in and interacts with Asp194 in interior of molecule. This amino group must be protonated for enzyme to be active. 3. Interaction between these +ve and -ve charges in a non-polar region triggers conformational changes: Met192 moves from buried (in zymogen) to surface (in enzyme). R ...
... 2. New +NH3 turns in and interacts with Asp194 in interior of molecule. This amino group must be protonated for enzyme to be active. 3. Interaction between these +ve and -ve charges in a non-polar region triggers conformational changes: Met192 moves from buried (in zymogen) to surface (in enzyme). R ...
PDF
... maturation. However, in the ensuing 30-60 min a protein of approximately 38xlO 3 M r became dephosphorylated (marked 1 on Fig. 3A) and two others (designated 2a and 2b) became more heavily labelled. New protein synthesis is not required for the observed early phosphorylation modifications. The possi ...
... maturation. However, in the ensuing 30-60 min a protein of approximately 38xlO 3 M r became dephosphorylated (marked 1 on Fig. 3A) and two others (designated 2a and 2b) became more heavily labelled. New protein synthesis is not required for the observed early phosphorylation modifications. The possi ...
AMP-activated/SNF1 protein kinases: conserved guardians of
... know almost nothing about the properties of the enzyme from S. pombe, but this structure does define the core interactions between the three subunits, which are likely to be conserved between different eukaryotes. The α-subunits: catalytic subunits. Two α-subunit iso forms (α1/α2) are encoded by di ...
... know almost nothing about the properties of the enzyme from S. pombe, but this structure does define the core interactions between the three subunits, which are likely to be conserved between different eukaryotes. The α-subunits: catalytic subunits. Two α-subunit iso forms (α1/α2) are encoded by di ...
Proteins that contain a functional Z-DNA
... and alternative splicing (3,4). The short form of ADAR1 (ADAR1 p110; ADAR1p110) is constitutively expressed and localizes mainly to the nucleus, whereas the long form of ADAR1 (ADAR1p150; ADAR1p150) is interferon-inducible and shuttles between the nucleus and cytoplasm. However, the localization of ...
... and alternative splicing (3,4). The short form of ADAR1 (ADAR1 p110; ADAR1p110) is constitutively expressed and localizes mainly to the nucleus, whereas the long form of ADAR1 (ADAR1p150; ADAR1p150) is interferon-inducible and shuttles between the nucleus and cytoplasm. However, the localization of ...
PI3K signalling during influenza A virus infections
... for binding to p85 SH2 domains [9]. Mutational studies reveal that both Tyr-89 and Met-93 are essential for the interaction of NS1 with p85β [6]. However, structural analysis of the homodimeric NS1 effector domain indicates that Tyr-89 is exposed in a cleft formed between the two monomers, whereas M ...
... for binding to p85 SH2 domains [9]. Mutational studies reveal that both Tyr-89 and Met-93 are essential for the interaction of NS1 with p85β [6]. However, structural analysis of the homodimeric NS1 effector domain indicates that Tyr-89 is exposed in a cleft formed between the two monomers, whereas M ...
This Article Abstract Full Text (PDF) Alert me when this article is cited
... (1, 2). Wza is an outer membrane lipoprotein that multimerizes to form ring-like structures resembling secretins for type II and type III protein secretion (13). HMW CPS is believed to cross the outer membrane through this complex. WzcCPS (involved in group 1 CPS assembly) is a tyrosine autokinase, ...
... (1, 2). Wza is an outer membrane lipoprotein that multimerizes to form ring-like structures resembling secretins for type II and type III protein secretion (13). HMW CPS is believed to cross the outer membrane through this complex. WzcCPS (involved in group 1 CPS assembly) is a tyrosine autokinase, ...
Protein Quality Control as a Strategy for Cellular Regulation
... misfolded proteins in the cell. This appears to be the case in yeast ERAD, where loss of either pathway (HRD or DOA) leads to increased UPR signaling, indicating higher levels of naturally produced ER misfolded proteins, and increased sensitivity to drugs that promote misfolding in the ER.23,24 Pres ...
... misfolded proteins in the cell. This appears to be the case in yeast ERAD, where loss of either pathway (HRD or DOA) leads to increased UPR signaling, indicating higher levels of naturally produced ER misfolded proteins, and increased sensitivity to drugs that promote misfolding in the ER.23,24 Pres ...
The UUAG-specific RNA Binding Protein, Heterogeneous Nuclear
... common groups of RNA binding proteins is the RBD class proteins (2). They possess a CS-RBD (consensus sequence-RNA binding domain) motif, which is typically 80 –90 amino acids. Two short sequences, RNP 2 octamer and RNP 1 hexamer, have been found to be conserved among different RBDs. Several RBDs ar ...
... common groups of RNA binding proteins is the RBD class proteins (2). They possess a CS-RBD (consensus sequence-RNA binding domain) motif, which is typically 80 –90 amino acids. Two short sequences, RNP 2 octamer and RNP 1 hexamer, have been found to be conserved among different RBDs. Several RBDs ar ...
Aminoacyl-tRNA synthetase complexes: beyond translation
... simultaneously, and only a subset of ARSs are involved. Although there is still some ambiguity about the stoichiometry and total number of components, at least nine different ARSs, including both class I and class II enzymes, have been consistently found in the mammalian complex: EPRS, IRS, LRS, MRS ...
... simultaneously, and only a subset of ARSs are involved. Although there is still some ambiguity about the stoichiometry and total number of components, at least nine different ARSs, including both class I and class II enzymes, have been consistently found in the mammalian complex: EPRS, IRS, LRS, MRS ...
Note 7.3 - Translation
... to produce the appropriate sequence and number of amino acids to be found in the polypeptide chain (primary protein). The ribosome is composed of two different sized parts; large and small ribosomal subunits, made up of a combination of ribosomal RNA and ribosomal protein. Each ribosome has two bind ...
... to produce the appropriate sequence and number of amino acids to be found in the polypeptide chain (primary protein). The ribosome is composed of two different sized parts; large and small ribosomal subunits, made up of a combination of ribosomal RNA and ribosomal protein. Each ribosome has two bind ...
anatomy of a feed tag - Westway Feed Products
... The names of the trace minerals are long and there are a lot of them, so an ingredient list can be lengthened and made imposing looking if they are included. But if the levels are not guaranteed, there is no way to know if any useful amount of the traces was included. It is a good idea to check the ...
... The names of the trace minerals are long and there are a lot of them, so an ingredient list can be lengthened and made imposing looking if they are included. But if the levels are not guaranteed, there is no way to know if any useful amount of the traces was included. It is a good idea to check the ...
Distribution and phylogeny of the bacterial translational GTPases
... factors that utilize GTP are called translational GTPases (trGTPases). Four large families of trGTPases - IF2/eIF5B, SelB/eIF2γ, EF-Tu/EF-1α and EFG/EF-2 - can be distinguished (Leipe et al. 2002). For each of those families one ancestral gene existed in the last universal common ancestor (LUCA) (Le ...
... factors that utilize GTP are called translational GTPases (trGTPases). Four large families of trGTPases - IF2/eIF5B, SelB/eIF2γ, EF-Tu/EF-1α and EFG/EF-2 - can be distinguished (Leipe et al. 2002). For each of those families one ancestral gene existed in the last universal common ancestor (LUCA) (Le ...
1 Function of the Arabidopsis kinesin-4, FRA1, requires
... thinner cell walls, reduced rate of pectin secretion and accumulation of Golgi-associated vesicles, which together indicate that FRA1 likely transports vesicles for secretion. In contrast, both microtubule plus-end dynamics and array organization are unaltered in this mutant (Zhu et al., 2015), demo ...
... thinner cell walls, reduced rate of pectin secretion and accumulation of Golgi-associated vesicles, which together indicate that FRA1 likely transports vesicles for secretion. In contrast, both microtubule plus-end dynamics and array organization are unaltered in this mutant (Zhu et al., 2015), demo ...
PDF - Bezanilla Lab
... (Fig. 1 B and C; Fig. S1B). Real-time RT-PCR analysis of class I silenced plants shows that all class I transcripts are reduced in expression (Fig. 1D). Overall, class I silenced plants have a 76% reduction in the total amount of class I transcripts. Although the plants are smaller, they retain high ...
... (Fig. 1 B and C; Fig. S1B). Real-time RT-PCR analysis of class I silenced plants shows that all class I transcripts are reduced in expression (Fig. 1D). Overall, class I silenced plants have a 76% reduction in the total amount of class I transcripts. Although the plants are smaller, they retain high ...
Improved detection and identification of low
... the pH range 4.5–5.2 on SDS gels. High sample loads of up to 3 mg BALF could be applied using the micropreparative method described without detectable streaking or loss of proteins from the gels. Of the 250 BALF spots detected by 2-D electrophoresis, 49 spots (corresponding to 28 different proteins) ...
... the pH range 4.5–5.2 on SDS gels. High sample loads of up to 3 mg BALF could be applied using the micropreparative method described without detectable streaking or loss of proteins from the gels. Of the 250 BALF spots detected by 2-D electrophoresis, 49 spots (corresponding to 28 different proteins) ...
Chemistry 501 Lecture 3 Amino Acids
... Clue to remember: PVT TIM HALL Proteinaceous AA − proteins consist of 20 AA. • Essential − organism is not able to synthesize these AA but ...
... Clue to remember: PVT TIM HALL Proteinaceous AA − proteins consist of 20 AA. • Essential − organism is not able to synthesize these AA but ...
Repositório da Universidade de Lisboa
... give the data a context from which evolutionary studies of complex systems can be achieved. To study the evolution of the vesicular trafficking system it’s necessary to know the phylogenetic profiles of it’s components, which is to say the organisms in which the components are present or absent. Rab ...
... give the data a context from which evolutionary studies of complex systems can be achieved. To study the evolution of the vesicular trafficking system it’s necessary to know the phylogenetic profiles of it’s components, which is to say the organisms in which the components are present or absent. Rab ...
Single Processing Center Models for Human Dicer and Bacterial
... Figure 2C, incubation of the wt Dicer with the 30 bp substrate containing either the upper or lower strand labeled at the 5⬘ terminus yielded series of processing products diagnostic of the enzyme approaching the substrate either from the label-containing (20–23 nt fragments) or opposite (9–12 nt fr ...
... Figure 2C, incubation of the wt Dicer with the 30 bp substrate containing either the upper or lower strand labeled at the 5⬘ terminus yielded series of processing products diagnostic of the enzyme approaching the substrate either from the label-containing (20–23 nt fragments) or opposite (9–12 nt fr ...
Leukaemia Section t(9;22)(q34;q11) in CML Atlas of Genetics and Cytogenetics
... however, 9q34-3’ABL always joins 22q11-5’BCR in true CML; the third chromosome and breakpoint is, at times, not random. In a way, masked Philadelphia chromosomes (see above) are also variants. ...
... however, 9q34-3’ABL always joins 22q11-5’BCR in true CML; the third chromosome and breakpoint is, at times, not random. In a way, masked Philadelphia chromosomes (see above) are also variants. ...
Protein 3D-structure analysis
... http://www.rcsb.org/pdb/explore/explore.do?structureId=1THC Similar structure: 2H6U What is the function of transthyretin, respectively of other proteins with similar structure? Have a look at the corresponding UniProt entries! How are they classified at CATH Use text search http://www.cathdb.info/c ...
... http://www.rcsb.org/pdb/explore/explore.do?structureId=1THC Similar structure: 2H6U What is the function of transthyretin, respectively of other proteins with similar structure? Have a look at the corresponding UniProt entries! How are they classified at CATH Use text search http://www.cathdb.info/c ...
Proteases and proteolytic cleavage of storage
... 30-40 kDa) has a weak acidic pi and is named a-chain, whereas the smaller more hydrophobic one (mol. wt. 20 kDa) has a strongly basic pi and is named /-chain. Both chains are linked by a disulphide bridge between cysteine residues at highly conserved positions in the a(amino acid residue 87) and /-c ...
... 30-40 kDa) has a weak acidic pi and is named a-chain, whereas the smaller more hydrophobic one (mol. wt. 20 kDa) has a strongly basic pi and is named /-chain. Both chains are linked by a disulphide bridge between cysteine residues at highly conserved positions in the a(amino acid residue 87) and /-c ...
View PDF - Faculty Web Sites at the University of Virginia
... Drcsopbilu, kinesin heavy chain is an essential gene product that probably transports axonal organeUes toward the periphery of the organism [26*]. The collective studies cited above Imply that the function of kinesin is to move many types of organelles, in most if not all cases toward microtubule pl ...
... Drcsopbilu, kinesin heavy chain is an essential gene product that probably transports axonal organeUes toward the periphery of the organism [26*]. The collective studies cited above Imply that the function of kinesin is to move many types of organelles, in most if not all cases toward microtubule pl ...
Molecular Microbiology
... HopZ T3SE family in the plant pathogen Pseudomonas syringae is used as a model to investigate the molecular basis underlying sequence diversification-enabled effector evolution. HopZ homologues belong to the YopJ family of T3Ses, which have been identified from both plant and animal pathogens (Arnol ...
... HopZ T3SE family in the plant pathogen Pseudomonas syringae is used as a model to investigate the molecular basis underlying sequence diversification-enabled effector evolution. HopZ homologues belong to the YopJ family of T3Ses, which have been identified from both plant and animal pathogens (Arnol ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.