ref. #28 of the TIBS article
... constructs were expressed in vitro in the presence of dog pancreas microsomes, and MGD values were obtained by determining the number of residues between a given reference residue at the lumenal end of the TMH and the glycosylation acceptor Asn needed to get half-maximal glycosylation. Calibration: ...
... constructs were expressed in vitro in the presence of dog pancreas microsomes, and MGD values were obtained by determining the number of residues between a given reference residue at the lumenal end of the TMH and the glycosylation acceptor Asn needed to get half-maximal glycosylation. Calibration: ...
Relaxosome function and conjugation regulation in Flike plasmids a
... et al., 2011). The RNA footprinting and cross-linking data, together with structural data from small-angle X-ray scat- ...
... et al., 2011). The RNA footprinting and cross-linking data, together with structural data from small-angle X-ray scat- ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... membranes. The vesicles had the same diameter as those observed in the cells. They had a characteristic structure, with an electron-dense core surrounded by an electrontranslucent layer. Some vesicles contained small electrontranslucent vesicle–like structures, and the content of the vesicle-like st ...
... membranes. The vesicles had the same diameter as those observed in the cells. They had a characteristic structure, with an electron-dense core surrounded by an electrontranslucent layer. Some vesicles contained small electrontranslucent vesicle–like structures, and the content of the vesicle-like st ...
SB401, a pollen-specific protein from Solanum berthaultii
... Figure 4. SB401 protein bundles MTs. Samples containing 0.5 lM pre-formed taxol-stabilized rhodamine-conjugated MTs were incubated with varying concentrations of SB401 proteins at room temperature for 5 min, and then observed under a confocal microscope. (a) Single-filament MTs are scattered through ...
... Figure 4. SB401 protein bundles MTs. Samples containing 0.5 lM pre-formed taxol-stabilized rhodamine-conjugated MTs were incubated with varying concentrations of SB401 proteins at room temperature for 5 min, and then observed under a confocal microscope. (a) Single-filament MTs are scattered through ...
Mutational Analysis of Synaptobrevin Transmembrane Domain
... units of CAT activity, which is slightly lower than synaptobrevin. All of the synaptobrevin and syntaxin constructs gave higher CAT activity than the GpA G83I mutant. This suggests that there is some association. However, none of these constructs gave more than 25% of the CAT activity seen for the G ...
... units of CAT activity, which is slightly lower than synaptobrevin. All of the synaptobrevin and syntaxin constructs gave higher CAT activity than the GpA G83I mutant. This suggests that there is some association. However, none of these constructs gave more than 25% of the CAT activity seen for the G ...
Dairy 17 - Milk Urea Nitrogen
... cow. For example, low urea contents are associated with low amounts of dietary protein and/ or degradable protein, for example, low D-value mature grass or silage or diets containing large quantities of maize silage without appropriate protein supplementation. High urea concentrations are associated ...
... cow. For example, low urea contents are associated with low amounts of dietary protein and/ or degradable protein, for example, low D-value mature grass or silage or diets containing large quantities of maize silage without appropriate protein supplementation. High urea concentrations are associated ...
A Molecular Switch for Targeting between Endoplasmic Reticulum
... expressed in different cell types. Depending on the targeting domain or motif an AKAP carries, it can bring PKA to different subcellular locations such as the nucleus, plasma membrane, endoplasmic reticulum (ER), Golgi, mitochondria, microtubules, etc. (6 – 8). DAKAP1 (also known as AKAP1, S-AKAP84, ...
... expressed in different cell types. Depending on the targeting domain or motif an AKAP carries, it can bring PKA to different subcellular locations such as the nucleus, plasma membrane, endoplasmic reticulum (ER), Golgi, mitochondria, microtubules, etc. (6 – 8). DAKAP1 (also known as AKAP1, S-AKAP84, ...
REVIEWS
... The structure of the AcrB trimer is shown in Fig. 4. The periplasmic domain adopts a subtly different conformation in each subunit of the trimer40,42. Each contains a potential substrate-recognition site and structures with bound drugs have been obtained40. However, only one site (the binding site; ...
... The structure of the AcrB trimer is shown in Fig. 4. The periplasmic domain adopts a subtly different conformation in each subunit of the trimer40,42. Each contains a potential substrate-recognition site and structures with bound drugs have been obtained40. However, only one site (the binding site; ...
PDF - Oxford Academic - Oxford University Press
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
assembly of integral membrane proteins from the periplasm into the
... OMPs are Skp, SurA, DegP, and FkpA. All of the currently discovered soluble periplasmic folding factors are bifunctional. Skp binds unfolded OMPs and LPS; SurA and FkpA have chaperone function and—independent of the chaperone function—PPIase activity. DegP is a protease and a chaperone. From the per ...
... OMPs are Skp, SurA, DegP, and FkpA. All of the currently discovered soluble periplasmic folding factors are bifunctional. Skp binds unfolded OMPs and LPS; SurA and FkpA have chaperone function and—independent of the chaperone function—PPIase activity. DegP is a protease and a chaperone. From the per ...
chemistry 1000 - U of L Class Index
... On March 15, 2007, the US FDA learned that some brands of pet food were making animals very ill – even killing some! This was traced to vegetable proteins imported from China which were found to contain melamine. There was a large-scale recall of pet food and the responsible individuals were indicte ...
... On March 15, 2007, the US FDA learned that some brands of pet food were making animals very ill – even killing some! This was traced to vegetable proteins imported from China which were found to contain melamine. There was a large-scale recall of pet food and the responsible individuals were indicte ...
HRP - WordPress.com
... water treatment, reagent for organic synthesis, immunoassays, and diagnostic kits. Prior work shows the protein can be isolated from horseradish root and remain active over time. This project continued work towards developing a sequence of protein purification steps for use as an undergraduate bioch ...
... water treatment, reagent for organic synthesis, immunoassays, and diagnostic kits. Prior work shows the protein can be isolated from horseradish root and remain active over time. This project continued work towards developing a sequence of protein purification steps for use as an undergraduate bioch ...
Full-Text PDF
... For example, contractile signaling in the gastrointestinal smooth muscle [3] can include acetylcholine stimulation of M2 and M3 muscarinic receptors, ATP/UTP stimulation of P2Y2 receptors, S1P stimulation of S1P2 receptors and NPY/PP stimulation of Y2/Y4 receptors. In the vascular smooth muscle [5], ...
... For example, contractile signaling in the gastrointestinal smooth muscle [3] can include acetylcholine stimulation of M2 and M3 muscarinic receptors, ATP/UTP stimulation of P2Y2 receptors, S1P stimulation of S1P2 receptors and NPY/PP stimulation of Y2/Y4 receptors. In the vascular smooth muscle [5], ...
An immunoassay for atrazine using tunable immunosorbent Jae-Young Kim, Ashok Mulchandani,
... antibody react under homogeneous, as opposed to heterogenous in ELISA, conditions and the immunocomplex is separated from the solution by a simple thermal precipitation. Typically, thermally reversible polymers such as poly-N-isopropylacrylamide are chemically conjugated to the antibodies. This is t ...
... antibody react under homogeneous, as opposed to heterogenous in ELISA, conditions and the immunocomplex is separated from the solution by a simple thermal precipitation. Typically, thermally reversible polymers such as poly-N-isopropylacrylamide are chemically conjugated to the antibodies. This is t ...
CitA (citrate) and DcuS (C 4 -dicarboxylate) sensor kinases in
... efficiency similar to CitA of E. coli (Fig. 3B) indicating that citrate transport and citrate lyase ...
... efficiency similar to CitA of E. coli (Fig. 3B) indicating that citrate transport and citrate lyase ...
The standard procedure starts with a set of sequences
... such an alignment. This number is called similarity score or alignment score and serves to evaluate the significance of a potential motif occurrence. Like most similarity search techniques, a protein database search with a profile returns a sorted list of potential matches ranked by a quality score. ...
... such an alignment. This number is called similarity score or alignment score and serves to evaluate the significance of a potential motif occurrence. Like most similarity search techniques, a protein database search with a profile returns a sorted list of potential matches ranked by a quality score. ...
Molecular Clocks
... The rate of this reaction is preset by the sequence and structure of the peptide or protein and associated peptides or proteins as genetically specified in the DNA. The rate may also be modified by changes in protein structure and solvent conditions in-vivo. This rate can be set to have a half-time ...
... The rate of this reaction is preset by the sequence and structure of the peptide or protein and associated peptides or proteins as genetically specified in the DNA. The rate may also be modified by changes in protein structure and solvent conditions in-vivo. This rate can be set to have a half-time ...
ref. #27 of the TIBS article
... Introduction Integral membrane proteins are thought to come in two basic varieties, built, respectively, on a helix bundle and a b-barrel architecture (von Heijne, 1996). The helix bundle design with its long, hydrophobic transmembrane a-helices (TMHs) is particularly simple, yet detailed studies of ...
... Introduction Integral membrane proteins are thought to come in two basic varieties, built, respectively, on a helix bundle and a b-barrel architecture (von Heijne, 1996). The helix bundle design with its long, hydrophobic transmembrane a-helices (TMHs) is particularly simple, yet detailed studies of ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
... from the bacterial cellulose synthase but is highly conserved among plant CesAs (Sethaphong et al. 2013; Pear et al. 1996). Although it is most logical for the catalytic domain to face the cytosol, where it would be most accessible to the substrate, the topological model of the protein in Figure 1 h ...
... from the bacterial cellulose synthase but is highly conserved among plant CesAs (Sethaphong et al. 2013; Pear et al. 1996). Although it is most logical for the catalytic domain to face the cytosol, where it would be most accessible to the substrate, the topological model of the protein in Figure 1 h ...
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and
... FASTA alignment in the Structural Database of Allergenic Proteins (SDAP) (Fermi.utmb.edu/SDAP). Allergenicity profile was assessed by combination of different parameters: hydrophobicity, antigenicity and SASA [9]. Values of absolute surface area (ASA) of each residue were also calculated by DSSP pro ...
... FASTA alignment in the Structural Database of Allergenic Proteins (SDAP) (Fermi.utmb.edu/SDAP). Allergenicity profile was assessed by combination of different parameters: hydrophobicity, antigenicity and SASA [9]. Values of absolute surface area (ASA) of each residue were also calculated by DSSP pro ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.