GFP (Green fluorescent protein)
... • Someone might be saying – why?who cares about this little green protein from a jellyfish? But it turns out with amazingly useful in scientific research, because it allows us to look directly into the inner workings of cells. It is easy to find out where GFP is at any given time: you just have to s ...
... • Someone might be saying – why?who cares about this little green protein from a jellyfish? But it turns out with amazingly useful in scientific research, because it allows us to look directly into the inner workings of cells. It is easy to find out where GFP is at any given time: you just have to s ...
Supplementary Figure 2. Further examples of large differences
... score remains at 0.333. B) Ribbon diagram of CASP8 target T0426. This example shows a hypothetical prediction highlighting the difference between the MCC and BDT scores for the predicted residues close to the binding site, which were not included in the official observed subset. For the purposes of ...
... score remains at 0.333. B) Ribbon diagram of CASP8 target T0426. This example shows a hypothetical prediction highlighting the difference between the MCC and BDT scores for the predicted residues close to the binding site, which were not included in the official observed subset. For the purposes of ...
Systematic comparison of single-chain Fv antibody
... molecule for antibody-targeted therapies. A combination of anti-CD19, −CD22, and -CD38saporin ITs (3BIT cocktail) has been shown previously to cure severe combined immunodeficient mice xenografted with the human B-cell lymphoma cell line Ramos, resulting in 100% disease-free survivors at 300 days ...
... molecule for antibody-targeted therapies. A combination of anti-CD19, −CD22, and -CD38saporin ITs (3BIT cocktail) has been shown previously to cure severe combined immunodeficient mice xenografted with the human B-cell lymphoma cell line Ramos, resulting in 100% disease-free survivors at 300 days ...
Adaptation and Protein Quality Control Under Metalloid
... either through direct interactions with arsenite on unfolded peptides or because arsenite interferes with chaperone activity. (2) The physiological effects are likely to involve aberrant protein-protein interactions. (3) The cell responds by down-regulating aggregationprone proteins. Implications. R ...
... either through direct interactions with arsenite on unfolded peptides or because arsenite interferes with chaperone activity. (2) The physiological effects are likely to involve aberrant protein-protein interactions. (3) The cell responds by down-regulating aggregationprone proteins. Implications. R ...
Articulins and epiplasmins - Journal of Cell Science
... Each of these three filamentous systems is composed of a distinct class of cytoskeletal proteins and their associated proteins. Microfilaments are made of actin, microtubules of tubulins and the intermediate filaments of intermediate filament (IF) proteins. IF proteins show the greatest diversity, a ...
... Each of these three filamentous systems is composed of a distinct class of cytoskeletal proteins and their associated proteins. Microfilaments are made of actin, microtubules of tubulins and the intermediate filaments of intermediate filament (IF) proteins. IF proteins show the greatest diversity, a ...
A New Subfamily of Major Intrinsic Proteins in
... FIG. 4.—Part of the interior structure of GlpF, showing the asparagines 68 and 203 in the NPA boxes and some of the discussed amino acid residues as ball-and-stick models (Fu et al. 2000). Oxygen, nitrogen, and carbon atoms are depicted in dark, intermediate, and light gray, respectively (in the on- ...
... FIG. 4.—Part of the interior structure of GlpF, showing the asparagines 68 and 203 in the NPA boxes and some of the discussed amino acid residues as ball-and-stick models (Fu et al. 2000). Oxygen, nitrogen, and carbon atoms are depicted in dark, intermediate, and light gray, respectively (in the on- ...
Population Biology of the First Replicators: On
... as a template catalyst for the polymerization of a complementary strand or it may be degraded by hydrolysis into its mononucleotide components. The template reaction is the birth process of the replicator, so let bt be the rate of polymerization of complementary strands per unit time. The hydrolysis ...
... as a template catalyst for the polymerization of a complementary strand or it may be degraded by hydrolysis into its mononucleotide components. The template reaction is the birth process of the replicator, so let bt be the rate of polymerization of complementary strands per unit time. The hydrolysis ...
Computational and genetic evidence that different structural
... axes of TMH5 and 6 (called hereafter the “down” conformation) (Fig. 2). Given the small energy barrier between these two conformations (approximately 10 kcal mol–1), the linker might adopt either conformation in vivo. Although folding only two of the eight predicted TMHs presents an underconstrained ...
... axes of TMH5 and 6 (called hereafter the “down” conformation) (Fig. 2). Given the small energy barrier between these two conformations (approximately 10 kcal mol–1), the linker might adopt either conformation in vivo. Although folding only two of the eight predicted TMHs presents an underconstrained ...
The structure of secondary cell wall polymers: how
... Schäffer, 2003; Sleytr, 1978; Sleytr et al., 1996, 2002), novel aspects of a group of ‘non-classical’ SCWPs have emerged with regard to their structure and function. S-layers are generally composed of identical (glyco)protein species forming regular two-dimensional, lattices on bacterial cell surfa ...
... Schäffer, 2003; Sleytr, 1978; Sleytr et al., 1996, 2002), novel aspects of a group of ‘non-classical’ SCWPs have emerged with regard to their structure and function. S-layers are generally composed of identical (glyco)protein species forming regular two-dimensional, lattices on bacterial cell surfa ...
Cellular Disulfide Bond Formation in Bioactive Peptides and
... cofactor [73] which enables the generation of disulfide bonds. It also contains thioredoxin [74] motifs which aid in transferring the disulfide to the substrate protein [75]. In mammals, cells secrete QSOXs into the extracellular space after modification by the Golgi complex [76]. Human QSOX1 has tw ...
... cofactor [73] which enables the generation of disulfide bonds. It also contains thioredoxin [74] motifs which aid in transferring the disulfide to the substrate protein [75]. In mammals, cells secrete QSOXs into the extracellular space after modification by the Golgi complex [76]. Human QSOX1 has tw ...
The paradox of elongation factor 4: highly conserved, yet of no
... of E-tRNA: upon back-translocation, the P site will be re-occupied by deacylated tRNA from the E site and 30S subunit P site is therefore maintained by a tRNA, which is a prerequisite for the ribosome to maintain the correct reading frame in all intermediate states of translocation [6]. EF4•GTP bind ...
... of E-tRNA: upon back-translocation, the P site will be re-occupied by deacylated tRNA from the E site and 30S subunit P site is therefore maintained by a tRNA, which is a prerequisite for the ribosome to maintain the correct reading frame in all intermediate states of translocation [6]. EF4•GTP bind ...
Distributed Representations for Biological Sequence Analysis
... classification task, a word embeddings based approach offers other advantages. First, the size of the data vector obtained by using embeddings is much smaller in comparison to the original sequence, thus making the vectors obtained amenable for various machine learning algorithms. Indeed, one needs ...
... classification task, a word embeddings based approach offers other advantages. First, the size of the data vector obtained by using embeddings is much smaller in comparison to the original sequence, thus making the vectors obtained amenable for various machine learning algorithms. Indeed, one needs ...
Translocation of Globin Fusion Proteins across the Endoplasmic
... We have previously shown that the lactamase signal sequence together with the first five amino acids of authentic lactamase is sufficient to direct globin to the lumen of microsomal vesicles in cell-free systems (9). We initiated the present study by extending these initial observations using a cons ...
... We have previously shown that the lactamase signal sequence together with the first five amino acids of authentic lactamase is sufficient to direct globin to the lumen of microsomal vesicles in cell-free systems (9). We initiated the present study by extending these initial observations using a cons ...
TRYPSIN / LYS
... that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chymotrypsin-like specificity (1). Promega Trypsin has been modified by reductive methyl ...
... that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chymotrypsin-like specificity (1). Promega Trypsin has been modified by reductive methyl ...
Similarities and Differences in the Glycosylation Mechanisms in
... exclusively carry O-glycans. Then, in the early years of the 21st century, groundbreaking research on the bacterial pathogen Campylobacter jejuni, showed that this prokaryote has a general N-glycosylation system [3, 4]. It soon became clear that all three domains of life (Eukarya, Bacteria, and Arch ...
... exclusively carry O-glycans. Then, in the early years of the 21st century, groundbreaking research on the bacterial pathogen Campylobacter jejuni, showed that this prokaryote has a general N-glycosylation system [3, 4]. It soon became clear that all three domains of life (Eukarya, Bacteria, and Arch ...
Factor VIlI-Related Protein Circulates in Normal
... fresh plasma was anticoagulated with citrate or heparin and kept at 37#{176}C was initiated, or hepanin ...
... fresh plasma was anticoagulated with citrate or heparin and kept at 37#{176}C was initiated, or hepanin ...
Efficiency assay of detergent removal columns on - G
... respectively. Every second, a TOF MS precursor ion spectrum was accumulated, followed by three product ion spectra, each for 3 s. LC‐MS/MS: Nano‐LC was performed with an nano 2D LC (Eksigent) equipped with a Dionex C18 PepMap100 column (75 µm i.d.) flowing at 200 nL/min. Peptides (5 µL injections) w ...
... respectively. Every second, a TOF MS precursor ion spectrum was accumulated, followed by three product ion spectra, each for 3 s. LC‐MS/MS: Nano‐LC was performed with an nano 2D LC (Eksigent) equipped with a Dionex C18 PepMap100 column (75 µm i.d.) flowing at 200 nL/min. Peptides (5 µL injections) w ...
Translocation of proteins across archaeal cytoplasmic membranes
... vivo, and in vitro analyses of archaeal cellular processes have not only led to a better understanding of life under extreme conditions, but have also provided novel insights into bacterial and eukaryotic cellular function. One such cellular process is that of protein translocation. 1.2. Protein tra ...
... vivo, and in vitro analyses of archaeal cellular processes have not only led to a better understanding of life under extreme conditions, but have also provided novel insights into bacterial and eukaryotic cellular function. One such cellular process is that of protein translocation. 1.2. Protein tra ...
Gene Section ABL (v-abl Abelson murine leukemia viral oncogene homolog 1)
... c-ABL exhibit a permanent nuclear and cytoplasmic shuttling activity, driven by 3 nuclear localisation signals (NLS) and a single nuclear export signal (NES) close to the C-terminal region. Recent data suggest that nuclear and cytoplasmic ABL may have different functions. 1-Nuclear c-ABL plays a maj ...
... c-ABL exhibit a permanent nuclear and cytoplasmic shuttling activity, driven by 3 nuclear localisation signals (NLS) and a single nuclear export signal (NES) close to the C-terminal region. Recent data suggest that nuclear and cytoplasmic ABL may have different functions. 1-Nuclear c-ABL plays a maj ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.