Download Curriculum Vitae

Curriculum Vitae
Katrin Alice Mooslehner-Allen
Mill Cottage, Mill Hill, Weston Colville,
Cambridge, CB21 5NY
Home: 0044 1223 290986
Mob: 0044 7971 988691
Email: [email protected]
Education and Qualifications
1989
University of Hamburg PhD in the Department of Virology in the Heinrich-PetteInstitute on “Retroviral integration in transgenic mice” marked “sehr gut”,
identifying novel genes active during the process of retrovirus integration.
1985
University of Hamburg MSc degree in Biochemistry as main subject and
microbiology and botany as additional subjects marked “sehr gut” (equivalent to
A).
1977
Abitur (Allgemeine Hochschulreife) des Landes Schleswig-Holstein
11 subjects analogous to GCSE and A levels in the UK.
Research and Professional Experience
2005
Post Doctoral Research Associate with Daniolabs Ltd now Summit plc to
develop zebrafish models for neurodegenerative diseases.
2004
Post Doctoral Research Associate in MRC-LMB, Neurobiology Division
to analyse P2X channel transgenic mice.
2002
Post Doctoral Research Associate in the Hutchison/MRC Research Centre
to work on a mouse mutant in a trithorax group gene in Dr S Aparicio’s
Group.
1994
Part time and later Full Time Research Scientist at the Babraham Institute
working for the Parkinson’s disease Society UK on developing a mouse
model for Parkinson’s disease.
1992
Part time Research Assistant employed by the University of Cambridge.
1990
EMBO Fellowship with Dr A Surani: Molecular analysis of mutations in
transgenic mice generated by insertions of a LacZ enhancer trap construct and
methylation as an epigenetic modification influenced by genetic modifiers
behaving differently on different genetic backgrounds.
Skills
Molecular Biology: Mapping and cloning of mouse mutations using lambda and cosmid clones,
in vivo and in vitro analysis of gene function in mutant versus wild type mice and zebrafish,
which included the generation and analysis of transgenic lines. Gene expression studies on RNA
level (preparation of samples for expression arrays, RT PCR, Northern Blot Analysis, in situ
hybridization) and protein level (Western Blot analysis and Immunofluorescence). DAB and
fluorescent antibody staining on paraffin and cryo sections. Construct design for the production
of transgenic disease models using in vitro mutagenesis and long range PCR.
Mouse embryo and cell culture: superovulation, collection of fertilized oocytes by flushing
oviducts and collection of blastocysts by flushing the uterus, culture of 2 cell stages up to
blastocyst stages, embryonic stem cell culture and G418 selection to generate homozygous ES
cell lines, mouse fibroblast and embryonic carcinoma cell line cultures.
Mouse and Zebrafish models: I completed accredited training for personnel working under the
Animals (Scientific Procedures) Act 1986 in October 1998 to work with mice and in March 2005
to work with zebrafish. In the past I held personal licences for 3 different project grants to carry
out procedures on transgenic mice necessary for genotyping, behaviour tests (place preference,
beam walking, startle response), transplantation studies with ES cells and lentiviral injections. I
hold currently a personal licence under a Summit plc project grant to Dr A Fleming to carry out
procedures on zebrafish, necessary for the generation, maintenance and analysis of transgenic
lines designed to induce rod cell loss and to perform screens for compounds rescuing the cell loss.
Assay development: During my time at Daniolabs/summitplc I have been involved in the design
and troubleshooting of novel screening methods using zebrafish models of rod cell loss to find
compounds or drugs with therapeutic value for neuroprotection.
IT: Microsoft Office, Photoshop, Software knowledge associated with imaging work: analySIS ,
ImageJ, Quantity One for chemidoc; software knowledge associated with sequence analysis:
Lasergene, online access to artemis, NCBI tools, ENSEMBL database and UCSC Genome
Browser. Training on “Working with genomic Sequence” at the Wellcome Trust Sanger Institute
in April 2007.
Interpersonal: Supervision and teaching of graduate and PhD students
Languages: German-fluent written and spoken, English-fluent written and spoken
Publications
Mooslehner-Allen K, Pask D, Diekmann H, Fleming A, Goldsmith P, Rubinsztein D,
Roach A (2008) An Alzheimer’s disease model of rod cell loss in zebrafish. Manuscript
in preparation.
Yap D, Walker D, McKinney S, Turashvili G, Mooslehner-Allen K, Ruiz de Algara T,
Prentice L , Fee J, d’Anglemont de Tassigny X and Aparicio S (2008) Loss of function in
the histone regulator MLL5, results in male sterility due to terminal maturation defects of
spermatozoa. Submitted for publication.
Colebrooke RE, Chan PM, Lynch PJ, Mooslehner K, Emson PC (2007)
Differential gene expression in the striatum of mice with very low expression of the
vesicular monoamine transporter type 2 gene. Brain Res. 2007 Jun 4; 1152:10-6. Epub
2007 Mar 15.
Patel J, Mooslehner KA, Chan PM, Emson PC, Stamford JA (2003)
Presynaptic control of striatal dopamine neurotransmission in adult vesicular monoamine
transporter 2 (VMAT2) mutant mice. J Neurochem. 2003 May, 85 (4): 898-910.
Mooslehner KA, Chan PM, Xu W, Liu L, Smadja C, Humby T, Allen ND, Wilkinson
LS, Emson PC (2001)
Mice with very low expression of the vesicular monoamine transporter 2 gene survive
into adulthood: potential mouse model for parkinsonism. Mol Cell Biol. 2001 Aug 21
(16): 5321-31.
Mooslehner KA, Allen ND (1999)
Cloning of the mouse organic cation transporter 2 gene, Slc22a2, from an enhancer-trap
transgene integration locus. Mamm Genome. 1999 Mar, 10 (3): 218-24.
Xu W, Liu L, Mooslehner K, Emson PC (1997)
Structural organization of the human vesicular monoamine transporter type-2 gene and
promoter analysis using the jelly fish green fluorescent protein as a reporter. Brain Res
Mol Brain Res. 1997 Apr, 45 (1): 41-9.
Mooslehner K, Allen N, (1992) Imprinting, transgene methylation and genotype-specific
modification. Dev. Biol. 3:87−89.
Mooslehner K., Müller U, Karls U, Hamann L., Harbers K. (1991) Structure and expression of a
gene encoding a putative GTP-binding protein identified by provirus integration in a transgenic
mouse strain. Mol Cell Biol. 1991 Feb;11(2):886-93
Mooslehner K. , Karls U, Harbers K. (1990)
Retroviral integration sites in transgenic Mov mice frequently map in the vicinity of transcribed
DNA regions.
J Virol. 1990 Jun; 64(6):3056-8.
Kratochwil K, von der Mark K., Kollar EJ, Jaenisch R, Mooslehner K, Schwarz M, Haase K,
Gmachl L, Habers K (1989).
Retrovirus-induced insertional mutation in Mov13 mice affects collagen I expression in a tissuespecific manner.
Cell. 1989 Jun 2;57(5):807-16.
Mooslehner K, Harbers K. (1988)
Two mRNAs of mouse pro alpha 1(I) collagen gene differ in the size of the 3'-untranslated
region.
Nucleic Acids Res. 1988 Jan 25; 16(2):773
Patent
The VMAT2 mutants have been patented as a novel mouse model for pathologies involved in
monoamine dysfunction: UK patent reference number: SMW/CP5762422. GB patent:
GB99200074.3. International patent: PCT/GB00/03274
Presentations
2008
Joint Meeting of Neuroscience for Clinicians 15 & Brain Repair Spring School in
Cambridge UK, poster presentation: K A Mooslehner-Allen, D Pask, H Diekmann, A
Fleming, P Goldsmith, D Rubinsztein, A Roach: “A zebrafish model of Alzheimer’s
Disease”.
2006
DanioLabs company presentation, 30 min talk on: “A zebrafish model of
Alzheimer’s Disease”.
Poster presentation at the conference for “Therapeutic Strategies against
Neurodegenerative Conditions” in Washington: P Cuddon, H Diekmann, D Pask, K
Allen, O Anitchik, A Fleming, P Goldsmith, D Rubinsztein: “Modelling
Neurodegenerative Disease in Zebrafish”.
2002
chalk talk series in the Hutchison/MRC Research Centre, 30 min talk on: A mouse
mutant in a trithorax group gene”
2001
Poster presentation in Dublin: Patel, J., Mooslehner, K.A., Chan, P.M., Emson, P.C.,
Stamford, J.A. Striatal Dopamine Neurotransmission in Adult Vesicular Monoamine
Transporter 2 (VMAT2) Mutant Mice: Voltammetric Assessment. Monitoring
Molecules in Neuroscience, Proceedings of the 9th international conference on in vivo
methods, pp 253-254. ISBN: 1-902277-47-3. Eds: O’Connor, W.T., Lowry, J.P.,
O’Connor, J.J. and O’Neil, R.D.
2000
Neuroscience Meeting in New Orleans:
Chan, P.M., Mooslehner, K.A., Keverne, E.B. & Emson P.C. (2000) Studies of basal
and amphetamine stimulated dopamine release in the dorsal striatum of a vesicular
monoamine transporter (VMAT2) mutant mouse. Soc. Neurosci. Abs. 26, 344.7.
FENS 2000 in Brighton:
Poster presentations: “Mice lacking expression of the vesicular monoamine
transporter 2 gene: A novel mouse model for disorders of monoamine
neurotransmission” and “A novel mouse model for Parkinson’s Disease: A neural
and neurochemical analysis of a mutation in a gene implicated in monoamine storage
in the brain”.
Babraham Annual Labtalks:
30 min talk on: “ A novel transgenic mouse with severely impaired monoamine
neurotransmission”.
1999
Cambridge Neuroscience Meeting
Poster presentation: “Mice lacking expression of the vesicular monoamine transporter
2 gene: A novel mouse model for disorders of monoamine neurotransmission”.
1998
TMR Euro-conference on Neuronal Transporters: Recent Advances and Future
Directions in Research. This included a poster presentation and also a 10min talk on
the “Cloning of the mouse organic cation transporter 2 gene Slc22a2”.
1997
Bayerische Julius-Maximilians-Universität Würzburg, Anatomisches Institut, invited
by Prof. Dr. H. Koepsell to give a talk on: ”Mutationen von Kationentransportern an
Mäusen”.
Names and contact details of 3 referees
Name
Dr Alan Roach,
Position
Senior Director of Biology
Address
Summit plc
91 Milton Park
Abingdon
Oxon.
OX14 4RY.
Telephone number: +44 (0)1235 443 910
E-mail address [email protected]
Do we have permission to contact this referee before the interview?
Yes
Second Referee
Name
Dr Sam Aparicio
Position
Department Head Molecular Oncology and Breast Cancer Program
Address
BC Cancer Research Centre
675 West 10th Avenue
Vancouver, BC
V5Z 1L3 Canada
Telephone number 1-604-675-8000
Fax: 1-604-675-8219
E-mail address [email protected]
Do we have permission to contact this referee before the interview?
Yes
Third Referee
Name
Dr Piers Emson
Position
Senior Affiliated Scientist
Address
Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT
Telephone number 01223 496503
E-mail address [email protected]
Do we have permission to contact this referee before the interview?
Yes