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Name _____________________________________________________________ Test Date _Tues, 11/16_______ UNIT 6 – THE WORKING CELL I. CELL BOUNDARIES (pp. 182,183) A. Cell Wall Cell walls are the outermost boundary in the cells of _plants___, _fungi___, and _bacteria___. _Protist___ cells may have cell walls but they are never found in _animal___ cells. The primary function of the cell wall is to provide _structure and support__. The cell wall does not regulate what _enters and leaves______ the cell. 1. Cell walls of _plants_____ are composed of _cellulose______ 2. Cell walls of _fungi_______ are composed of _chitin________ 3. Cell walls of _eubacteria___ are composed of _peptidoglycan_____ B. Cell Membrane Every cell is surrounded by a cell membrane. It helps to maintain _homeostasis______ in the cell by functioning as a _protective barrier__ between the cell and its environment. The cell membrane is _selectively permeable___ which means it only allows certain things to enter and exit the cell. The cell membrane is also called the _plasma membrane____. It is not a fixed sheet of molecules, but rather it is a _fluid____ structure composed of three biomolecule groups, _lipids____, _carbohydrates___, and _proteins______. 1. Lipids – There are two types of lipids that may make up the cell membrane: _phospholipids_______ and _cholesterol___ a. Phospholipid bilayer – The primary component of all cell membranes is the phospholipid bilayer. Phospholipids are unique molecules because they are partially _polar____ and partially _non-polar________. Each phospholipid has 2 _non-polar___ fatty acid tails at one end and a _polar___ phosphate head on the other end. A cell is surrounded by water and contains a high concentration of water. Water is _polar___. Polar is attracted to _polar_____, and non-polar is attracted to _non-polar_____ so the phospholipids form a _bilayer_________ so that the _polar phosphate heads__________ are directed toward the water inside & outside of the cell and the _non-polar fatty acid tails_______________ are directed toward each other. b. Cholesterol – Found in the cell membranes of _animal ________ cells only to help stabilize them. This is important because animal cells _do not have cell walls_____________________________. Cholesterol is a _non-polar ________ lipid so it is located between the _fatty acid tails________________ of the phospholipid bilayer. 2. Carbohydrates – Serve as “ID tags” to _identify cells__________ 3. Proteins – Embedded in the phospholipid bilayer. Serve as _channels_____ and _pumps______ for transport of specific molecules across the cell membrane. II. CELL TRANSPORT (pp. 183-189) A cell is a working unit and thus, needs to move materials in and out of the cell. There are two transport mechanisms: A. Passive Transport Does not require _energy____. Movement of substances from a _high____ concentration to _low______. There are three types of passive transport: 1. Diffusion – Due to the random movement of molecules in a _liquid_______ or _gas____. Many substances move across the _cell membrane____ by diffusion. 2. Facilitated Diffusion – Diffusion of _specific molecules______ through a _protein channel_______. Protein channels provide larger openings for larger molecules like _glucose____________. 3. Osmosis – Diffusion of _water________ through a _selectively permeable membrane________________. Cells must have a mechanism for counteracting the pressure osmosis can create - otherwise a cell could _swell and burst_____________ when it comes in contact with a dilute water solution. Cells control this osmotic pressure in one of three ways: a. Cell wall - _Physically prevents cell from expanding______ b. Contractile vacuole – Actively _pumps____ water out of cell in single-celled organisms without _cell walls____________________________ c. Blood – Cells are bathed in blood which is mostly _water____ to equalize osmotic pressure B. Active Transport Requires _energy___ in the form of _ATP_____. 1. Protein Pumps - Movement of _ions____ and small molecules against the concentration gradient; that is, from _low_____ concentration to _high___ concentration. 2. Endocytosis – “_Sucking In_________” Requires movement by cell membrane. In endocytosis, the cell membrane _surrounds and engulfs_____ particles; for example, _white_________ blood cells engulfing bacteria and other _pathogens________________ 3. Exocytosis – “_Spitting Out____________” Used by cell to release large amounts of material. In exocytosis, _vesicles___ containing substances move to _cell membrane_____, fuse with it, then _expel__ contents out of the cell; for example, secretion of hormones. III. CELL METABOLISM (pp. 50-53) A. _Metabolism____________ is the term used to describe the chemical processes occurring within a living cell that are necessary for maintenance of life. A key component of metabolism is the use of _enzymes_________________. Enzymes are _proteins_________ required for virtually every single chemical reaction in an organism; without enzymes, an organism would not be able to maintain _homeostasis__________. B. Function of Enzymes - Enzymes trigger chemical reactions in the body by acting as _catalysts_______________. An enzyme works by lowering the activation energy; that is, the energy required to _get the reaction started______. C. Characteristics of Enzymes 1. Enzymes work best within a narrow _pH___ and _temperature______ range. 2. Enzymes do not make a reaction happen that couldn’t happen on its own; they simply make the reaction occur much _faster________. 3. Enzymes are not _changed____ or _used up__________ in the reaction. The same enzyme may be used over and over again. 4. Enzymes are _substrate-specific____________. A substrate is the _reactant__________ Enzymes are substrate-specific because of their _shape_________. The substrate fits into a portion of the enzyme called the _active site_____. This fit between the active site of the enzyme and the substrate allows enzymes to be specific for a certain type of reaction. IV. CELL ENERGY (pp. 202, 203, 221-225) Energy is the _ability to do work______________. We obtain energy from _food______, but most energy originally comes from the _sun_____. Plants are able to capture the sun’s energy and use it to produce glucose, _C6 H12 O6______. This process, known as _photosynthesis_______, occurs in the _chloroplasts_________ of plant cells. The equation for photosynthesis is: _CO2 + H2O + energy → C6 H12 O6 + O2_____________ A. Chemical Energy The chemical energy in food (and all molecules) is stored in the _chemical bonds_____________. This is _potential______ energy. When the bonds are broken and the _electrons_______ are released, some energy is lost as _heat________, but a percentage of the potential energy can be converted to useable energy in the form of _ATP___. Food molecules cannot be used as a direct energy source for the cell because _the amoung of energy released would be wasteful and destructive______________________ B. ATP _Adenosine TriPhosphate____ is a _nucleotide composed of: Nitrogen base - _adenine_______ 5-Carbon sugar - _ribose______ _3__ phosphate groups Energy is stored in _phosphate – phosphate bonds_____. ATP works like a rechargeable battery. Cells are able to use and recycle ATP by breaking off or adding a _phosphate_ group. 1. Energy can be used by the cell when a phosphate bond is _broken________ and _ATP____ is converted to _ADP______ - _adenosine diphosphate_____________ 2. Energy is stored; that is, ATP is “recharged” by _adding_____ a phosphate group to _ADP___ to make _ATP_____. C. Cellular Respiration - _Aerobic______ Energy Production (pp. 221-223, 226-229) Cellular respiration is the breakdown of _glucose________ in the presence of _oxygen___ to “make” _ATP_____. In _eukaryotic_______ cells, this process occurs in the _mitochondria___________. The equation for cellular respiration is _ C6 H12 O6 + O2 → CO2 + H2O + energy (ATP)____________ In humans, 1. The oxygen required for cellular respiration is _inhaled_____ into the_lungs___, _diffuses _ into the _blood____, and is delivered to the _mitochondria________ of the body cells by _red blood cells_______. We exhale the waste products from cellular respiration, _ CO2_____ and _ H2O _____. 2. The glucose comes from the _digestion____ of _food_______. The glucose is absorbed into the _blood___ moves into body cells via _facilitated diffusion___. **TOTAL ATP GAIN IN CELLULAR RESPIRATION = 36 ATP per MOLECULE OF GLUCOSE ** D. Fermentation - _Anaerobic_____ Energy Production If oxygen is not available, some types of cells have a back-up mechanism for glucose metabolism called _fermentation___. The fermentation pathway only produces _2____ ATP per molecule of _glucose______. The two most common fermentation pathways used by cells are: 1. Lactic Acid Fermentation a. Human Muscle Cells – Occurs when demand on muscles exceeds supply of oxygen. As lactic acid builds up in the muscle cells, it is felt as _pain and fatigue_________. This is referred to as _oxygen debt__________ As activity slows, and oxygen is re-supplied, the muscle cells switch back to _cellular respiration________ and the lactic acid is sent to the _liver_________ to be broken down. b. Bacteria – There are some types of bacteria that carry out lactic acid fermentation in _anaerobic______ conditions. This is utilized by the dairy industry to produce _yogurt_____ and _cheese___. 2. Alcoholic Fermentation - When oxygen supplies are depleted, _yeast___ switch to alcoholic fermentation. This process is used commercially for _brewing and baking____ **TOTAL ATP GAIN IN FERMENTATION = 2 ATP per MOLECULE OF GLUCOSE **