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BIOLOGY CYTOLOGY Topic 1: Passive Diffusion 10. 1. Diffusion is the movement of molecules from an area of ______ concentration to an area of ______ concentration. 2. The diffusion of water across a selectively permeable membrane is called _____________. 3. Which of the following statements is true about diffusion? A) It is very rapid over long distances. B) It requires expenditure of energy by the cell. C) It is a passive process. D) It occurs when molecules move from a region of lower concentration to a region of higher concentration. E) It requires integral proteins of the cell membrane. 4. Explain the following terms: Hypertonic solution: Some erythrocyte cells are placed into a liquid that has a density of 1.1 like figure given above. Density of cells is 0.9. a) What will happen to red blood cells? b) If we place red blood cells into fresh water that has density of 0.7, what would be the result of experiment? 11. Sucrose cannot pass through the cell membrane, but water and urea can. Osmosis would cause red blood cells to shrink the most when immersed in which of the following solutions? A) a hypertonic sucrose solution B) a hypotonic sucrose solution C) a hypertonic urea solution D) a hypotonic urea solution E) pure water Isotonic Solution: Hypotonic Solution: 5. The internal solute concentration of a plant cell is about 0.8 M. To demonstrate plasmolysis it would be necessary to suspend the cell in what solution? A) distilled water B) 0.4 M C) 0.8 M D) 1.0 M E) any of the above 6. Sea water is dangerous to drink because____. A) one cup of sea water contains enough sodium to poison you B) sea water is hypertonic to your body tissues and drinking it will cause you to lose water by osmosis C) sea water is isotonic to your body fluids and you will absorb too much water, causing your cells to burst D) the salt causes hypertension and you will promptly die of a stroke E) it contains toxic levels of iodine 7. Match the situation to the description Situation Description a. The solution is above strength in solute. 12. What can be observed when we add a cell into the salty water (hypertonic solution)? 13. A hypertonic salt solution has a higher concentration of solutes than a blood cell. Explain what happens when a blood cell is placed in a hypertonic salt solution. 14. Which of the following factors increases rate of passive transport through the membrane, if the level of this factor increases? A) Molecular size B) Molecular weight C) Pore number D) Molecular charge E) Molecular type _____ 1. Two solutions are isotonic _____ 2. A solution is hypertonic b. The solutions are the same strenght. b) ……………………………………………………… _____ 3. A solution is hypotonic c. the solution is below strength in solute. d) ……………………………………………………… 17. List 4 factors that affect the rate of passive transport a) ……………………………………………………. c) ……………………………………………………… Topic 2: Active mechanisms 8. What are the effects of carrier proteins in diffusion? 1. What are the differences of active transport from passive transport? 9. If we put a plant cell into distilled water, it will __________. A) burst B) shrink C) swell D) sink E) swim Date: ____/_____________/ 2011 2. Which of the following enables a cell to pick up and concentrate a specific kind of molecule? A) Passive transport B) Diffusion C) Osmosis D) Active transport E) Exocytosis Signature: _____________________ BIOLOGY CYTOLOGY 4. If one cell makes passive diffusion but doesn’t make active transport, what can be said about this cell? 5. Write three types of endocytosis. 1. 2. 3. Substrate: Reactant: 3. What is the effect of biological catalyst on reactions? 4. Give 3 examples to enzymes. 5. If an enzyme is composed of only amino acids, this enzyme is called ______________. 6. The protein component of a complex enzyme is known as _______________. 7. Which of the following statements about enzymes is true? A) Enzymes raise the activation energy for reactions. B) Enzymes react with substrates (form chemical bonds) to form an enzyme-substrate complex, which irreversibly alters the enzyme. C) Most enzymes are the chains of amino acids. D) Only the most efficient enzymes can catalyze reactions in either direction. E) The more a reaction is heated, the faster the enzymes will function. 6. Which of the following cells uses the phagocytosis more than other cells? A) Neuron cells B) Epithelial cells C) Leucocytes D) Red Blood cells E) Muscle cells 7. Name the processes given below. 8. Which of the following environments or actions does NOT affect enzyme activity? A) heating the enzyme B) cooling the enzyme C) salt concentration D) pH E) all of the above can affect enzyme activity. 9. The activity of enzymes is decreased by some chemicals, called _________. A) Activators B) Inhibitors C) Coenzymes D) Cofactors E) Apoenzymes 10. Explain the effect of the temperature over enzyme activity? Topic 5: ATP and Metabolic Reactions 1. What is the universal form of energy that is used in organisms? 2. How do the living things use ATP? 3. How is the extra energy in body deposited? a) b) c) Topic 3: Metabolism 4. Label each part of the ATP molecule illustrated below. 1. Name the reactions given below as catabolic or anabolic. Protein synthesis : _________________ Glycogen formation : _________________ Lipid digestion : _________________ Photosynthesis : _________________ Cellular respiration : _________________ 2. How can we measure the energy of food? 3. What is the unit of energy that is used in body? Topic 4: Enzymes 1. Define the following terms. Enzyme: Date: ____/_____________/ 2011 4. Energy is released from ATP when ________. A) A phosphate group is added. B) Adenine bonds to ribose. C) ATP is exposed to sunlight. D) A phosphate group is removed. E) Ribose sugar is removed 6. Name the given reactions below. Protein+ (H2O) n-1 (amino acids) n Signature: _____________________ BIOLOGY CYTOLOGY Glucose + Glucose K+ + Cl- ATP c) Why did Engelmann use aerobic bacteria in experiment? d) Why do aerobic bacteria reproduce very less in green light? maltose + H2O 10. Label the parts of Chloroplast. KCl ADP + Pi + Energy C6H12O6 + 6O2 6H2O + 6CO2 + Energy Amino acid1 + amino acid2 +…aan Protein+(H2O)n-1 7. What are the important electron acceptors used in cells? 1. ________ is the ultimate source of energy for all living things. 11. Chlorophyll molecules are located in which part of the chloroplast? A) grana stacks B) stroma C) stomata D) plasma membrane E) golgi apparatus 3. What is the meaning of Autotrophs? Topic 7: Photosynthetic Reactions 4. How is the radiant energy that comes from sun captured by organisms? 1. Photosynthesis consist of two main stages, they are Topic 6: Photosynthesis 5. Which of the following organisms is an autotroph? A) Earthworm B) Mushroom C) Monkey D) Rose E) Decaying Bacteria 2. Where do the light-dependent reactions take place? 3. What is the photolysis? 4. The source of the oxygen produced by photosynthesis has been identified through experiments using radioactive tracers. The oxygen comes from ____. A) carbon dioxide B) glucose C) radioisotopes D) water E) light 6. Give examples for autotroph and heterotroph organisms. 8. Complete the following reaction. Sunlight 6____+ 6CO2 _________+ 6O2 Chlorophyll 9. The figure given below illustrates photosynthesis experiment of Engelmann which is studied by algae and aerobic bacteria. 5. The most important role of pigments in photosynthesis is to _____. A) capture light energy B) screen out harmful ultraviolet rays C) store energy in glucose molecules D) release energy from glucose molecules E) store energy in ATP 6. What are the end products of non-cyclic photophosphorylation? 7. ATP synthesis by light is called ________________ reaction. 9. Where are ATP and NADPH used? 10. What is the source of O2 that is removed during the non-cyclic photophosphorylation? Answer the questions according to figure. a) In which color the level of photosynthesis is the highest? b) In which color the level of photosynthesis is the lowest? Date: ____/_____________/ 2011 11. What is the role of NADP+ in photosynthesis? A) It assists chlorophyll in capturing light. B) It acts as the primary electron acceptor for the photosystems. C) As part of the electron transport chain, it manufactures ATP. D) It assists photosystem II in the splitting of water. E) It is reduced and then carries electrons to the Calvin cycle. Signature: _____________________ BIOLOGY CYTOLOGY 12. Why is the Calvin cycle reactions also called the light independent reactions? 13. If carbon dioxide is removed from a plant’s environment, what would you expect to happen to its production of high energy sugars? A) More sugars will be produced. B) Fewer sugars will be produced. C) The same number of sugars will be produced but without carbon dioxide. D) Carbon dioxide does not affect the production of high energy sugars in plants. 14. Complete the illustration of the overview of photosynthesis by writing the products and the reactants of the process, as well as the energy source that excites the electrons. 15. The Calvin cycle is a series of reactions that _____. A) assemble sugar molecules by fixing carbon B) convert light energy to chemical energy C) produce oxygen gas D) produce NADPH E) all of the above 16. Why is NADP+ needed in photosynthesis? A) It helps produce ATP from the light reactions. B) It absorbs light energy. C) It forms part of photosystem II. D) It is the primary electron acceptor. E) It forms NADPH to be used in the Calvin cycle. 18. What are the phases of Calvin’s cycle? 19. Which enzyme is used in Calvin’s cycle to catch CO2 from atmosphere? 20. Which of the following molecules is synthesized at the light phase of photosynthesis? A) ATP, NADPH2 B) Glucose di phosphate C) Ribulose di phosphate D) PGA E) CO2 Date: ____/_____________/ 2011 Signature: _____________________