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Biological Plant Science Unit 10 Review – Biological Engineering 10.1 Define Terms Match the following terms with their BEST definition listed below: A. Biological engineering B. biotechnology C. gene splicing D. genetic engineering E. genetically modified organism (GMO) _____ 1. The management of biological systems for the benefit of humanity. _____2. An advanced form of biotechnology; techniques involve gene slicing, replication, and transfer of genes to other organisms. _____3. The process of adding new genetic material within the DNA sequence of an organism. _____4. Artificially altering the genetic makeup of an organism. _____5. An organism that has been genetically engineered to achieve a desired trait. Match the following terms with their BEST definition listed below: A. E. coli B. particle gun C. transgenic plant D. vector E. restriction enzyme F. gel electrophoresis _____ 6. Material used to cut DNA. _____7. A device used to insert new genetic material into an organism. _____8. Bacteria that are commonly used to deliver new or altered genetic material in an organism. _____9. An agent that transfers DNA into a cell. _____10. Process used to sort DNA fragments. _____11. A plant that has been genetically altered by artificial means. 10.2 Discuss the meaning and process of genetic engineering 12. Explain the process of extracting DNA from a donor plant: 13. Explain the process of cutting DNA: 14. Explain the process of sorting DNA fragments: 15. Explain the process of joining isolated DNA fragments with new DNA: 16. Explain the process of using E. coli to insert DNA into host cells: 10.3 Identify and investigate issues associated with genetic engineering 17. Rank the following landmark events associated with genetic engineering in chronological order: _____ A. Watson and Crick identify the structure of DNA _____B. an agricultural animal is cloned. _____C. Gregor Mendel conducts research with peas. _____D. the Flavr-Savr Tomato is introduced. 18. Identify at least two major advantages to genetic engineering: 19. Identify at least two major concerns with genetic engineering: