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EWORP Alien Genetics Introduction: Background: Have you ever wondered why so many people can look different from each other, even when they are closely related? Our particular combination of features makes us unique. Just like in real life, the different combinations are created by the union of egg and sperm cells during fertilization. The alleles, (different versions of a gene), follow the law of segregation and separate into different sex cells during meiosis. Purpose: This activity is going to explore how organisms can vary greatly with just a few characteristics to choose from. Several different methods of genetic combinations will be used including: simple dominance, incomplete dominance, polygenic, and sex-linked. For this lab, you will be using the results of a coin flip to determine which allele goes into the sex cells that produce your baby alien. Materials: Lab handout Coin Procedure: Gene/Chromosome determination. For every different characteristic of the EWORP, you will be tossing a coin to determine the paternal and the maternal chromosome and the alleles on them. You will write the results of the tosses on the data table and then determine the genotype and phenotype of your EWORP. Some of the characteristics take more than one gene to determine the phenotype. Only one allele for each gene should go into a sex cell due to the law of segregation discovered by Gregor Mendel. Heads = Capital letter (dominant gene) Tails = Lowercase letter (recessive gene) 1 EWORP Traits: Genotypes and Phenotypes (10 points – Indicate which genotype) 1. EYE STYLE: Straight (SS or Ss) Droopy (ss) 2. SKULL SHAPE: Almond (AA or Aa) Balloon (aa) 3. LIPS Full (LL or Ll) Fluted (ll) 2 4. SKIN/FUR COLOR To determine the color of the skin or fur, assume three gene pairs are involved. This is called polygenic inheritance. Flip your coins to determine the genotype of the first pair of genes (AA, Aa or aa). Next flip for the second and third pairs (BB, Bb or bb) and (CC, Cc or cc). *You will flip your coin three times. Color is based on the number of CAPITAL LETTERS. See the chart below: # of CAPITALS 6 5 4 3 2 1 None COLOR Purple Blue Green Yellow Orange Red White or albino FURRINESS on the different body parts. Use the following genotypes for all areas: Fur (FF or Ff) No fur (ff) 5. ARMS ______ 6. FACE ______ 7. LEGS_____ 8. TORSO______ 9. EARS EWOK alien ears (EE or Ee) Mouse ears (ee) 3 10. EYE COLOR Eye color is also determined by polygenic inheritance, but this time it is only two pairs of genes. Flip to determine the first pair (CC, Cc or cc) and then determine the second pair (DD, Dd or dd). *You will flip your coin twice GENOTYPE CCDD CCDd CCdd CcDD CcDd Ccdd ccDD ccDd ccdd PHENOTYPE Black Brown Dark Blue Brown Brown Light Blue Dark Blue Green Pale or Ice Blue 11. HEAD HAIR The last two chromosomes for an organism are called the sex chromosomes: XX for females and XY for males. First, the paternal (male) will toss their coin to determine whether an X or Y goes into the sperm cell. If heads then it is an “X” chromosome, if tails, then you have a “y” chromosome. Then, automatically place a X in the egg cell (since females have only X chromosomes). Record in your data table. X X or Y (flip coin to determine) The traits located on the sex chromosomes are called sex-linked traits. “Pattern baldness” is a sex linked trait carried on the X chromosome, so it is called X-linked. Toss the coin to determine the allele on each of the X chromosomes for #11 in your data table. If your EWORP is female, you toss twice, if male, you only toss once. Heads = XH Tails = Xh Has head hair XHXH or XHXh female XHY male Hairless or bald XhXh female X hY male 4 12. NECK SIZE No neck (NN or Nn) Long neck (nn) 13. BODY SHAPE Round (QQ or Qq) Long (qq) 14. BELLY BUTTON Outie (OO or Oo) Innie (oo) 15. NUMBER OF FINGERS (Incomplete dominance is a “blend”. Use different capital letter for the alleles.) Four (FF) Three (FT) Two (TT) 5 16. FEET SHAPE Paddle feet (PP or Pp) Web feet (pp) 17. SUNSTANCE BREATHED (Incomplete dominance – like #15)) Breathes fire (FF) Breathes smoke (FW) 18. WARTS Attractive knee warts (WW or Ww) BTW: EWORPS like warts!! Squirts water (WW) Naked knees (ww) 6 Data Collection: When you are finished completing this data table, use the back of this paper to DRAW and NAME your EWORP offspring showing all the characteristics! TRAIT PATERNAL ALLELE in sperm cell MATERNAL ALLELE in egg cell EWORP GENOTYPE EWORP PHENOTYPE 1. Eye Style 2. Skull Shape 3. Lips 4. Skin/Fur Color 5. Arms 6. Face 7. Legs 8. Torso 9. Ears 10. Eye Color 11. Head Hair 12. Neck Size 13. Body Shape 14. Belly Button 15. # of Fingers 16. Feet Shape 17. Substance Breathed Out 18. Warts 7 Data Analysis: Answer the following questions below in complete sentences. 1. Briefly describe what your alien baby would look like at the end of this activity? 2. What was the probability of creating a boy or a girl alien baby? 3. Which parent actually determines the sex the child? 4. What is a genotype? What is a phenotype? How are they related? 5. In this activity, you saw that some alleles were dominant and some were recessive. Which alleles were expressed? What happened to the alleles that were not expressed? This situation aligns with which of Mendel’s laws? 6. You were asked to flip a coin to determine the various genotypes of your alien baby. Briefly describe a coin to determine the various genotypes of your alien baby. Briefly describe why you had to flip a coin. 7. For some traits such as hair color and eye color, you were required to flip the coin more than once. Why did you have the flip the coin multiple times in order to determine those traits? 8. What is the probability of getting a girl alien baby? What is the probability of getting another girl baby? What is the probability of getting three girl babies in a row? Justify your answer. 9. What is Mendel’s Law of Independent Assortment and how did it apply to this activity? How does this law apply to genetic diversity in real life? 10. Get together with a partner and examine your alien baby’s head color. Create a punnet square and identify the genotypic and phenotypic ratios of the offspring if your alien baby and your partner’s alien baby mated. What are the genotypic and phenotypic ratios of the offspring? Note: Head color is a sex-linked trait. Conclusion: Write 5-7 sentences about the type of data you gathered in this lab report. Address the following: Describe the role probability plays in genetics. Describe what you know about the Law of Independent Assortment and meiosis that contributes to such great genetic diversity on Earth. What is your evidence of this? Use data/information from this lab as evidence to support your answer. How does DNA contribute to what an organism looks like? How are traits passed down from generation to generation? Why are some traits more prevalent (common) than others? 8