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Transcript
Playing with Bacterial Plasmids (DNA) What is it? Background: 1928-Dr. Frederick Griffith studied two forms of S. pneumoniae Pathogenic strain (smooth)- polysaccharide coating Non-pathogenic (rough) Injected into mice: Rough: Mice lived Smooth: Mice died KILLED smooth: Mice lived A mix of killed smooth & rough, what should happen? How could this be? DNA had to have been exchanged between the two types of bacteria Transformation: uptake and expression of free DNA by cells Bacteria do this regularly Have surface proteins that recognize DNA and transport it into the cell If enough like the cell’s original, it is used (Recombination) If not, it is destroyed We are going to do this We will clone a plasmid that contains two special genes into E. coli: One that makes jellyfish glow (pGLOW) One that makes bacteria resistant to antibiotics Ampicillin
