Download Personal details Current position Name: Lilah Glazer, PhD E-mail:

Personal details
Name: Lilah Glazer, PhD
E-mail: [email protected]
Current position
Title: Postdoctoral scholar at the Woods Hole Oceanographic Institution (WHOI)
Department: biology
Work phone #: 508-289-2912
Work address: 45 Water Street, Woods Hole, 02543
Website: www.whoi.edu
Education
Institution and location
Degree
MM/YY
Field of study
Ben-Gurion University, Israel
B.Sc.
03/07
Biology
Ben-Gurion University, Israel
M.Sc.
09/08
Biology
Ben-Gurion University, Israel
Ph.D.
11/13
Biology
Dissertation title
Gastrolith matrix formation
in the red claw crayfish
Cherax quadricarinatuselucidation of protein
contents and functions
Past positions and teaching experience
2012-2013
Head of Biochemistry B course at the Ben-Gurion University Eilat campus
Teaching assistant at the Department of Life Sciences, Ben Gurion University:
2007-2013
Biochemistry B for 2nd year biology undergraduate students
2010-2012
Laboratory in physiology of reproduction for 3rd year undergraduate students
2008-2012
Personal instructor for 3rd year undergraduate research project
2007-2008
Vertebrate zoology for 1st year undergraduate students
Instructor at The Open University of Israel:
2011-2013
The cell: structure and function, advanced undergraduate Course
Teacher at Gedera Regional High School:
2012-2013
1
Biotechnology for senior level
Lilah Glazer
Awards and scholarships
2013 Postdoctoral Scholarship at the Woods Hole Oceanographic Institution. Woods Hole,
MA, USA, December 2013-June 2015
2010 Second prize for student oral presentation at the 9th International Marine Biotechnology
Conference, Qingdao, China, Oct 8-12, 2010
2010 A travel grant to participate in the 12th Congress of the International Society of
Invertebrate Reproduction and Development, Prague, Czech Republic, 2010.
2009 Dean’s Prize of Excellence for Graduate Studies. Faculty of Natural Sciences, BenGurion University of the Negev, Israel
2009 The Crustacean Society’s Best Student Oral Presentation Award, given at the annual
meeting of the Society for Integrative and Comparative Biology, Boston, MA, USA,
January 3-7, 2009.
Peer-reviewed Publications
1. Glazer L., Weil S., Tom M., Roth Z., Khalaila I., Mittelman B. and Sagi A. 2013.
Hemocyanin with phenoloxidase activity in the gastrolith matrix of the crayfish Cherax
quadricarinatus. Journal of Experimental Biology, 216:1898-1904.
2. Pamuru, R. R., Rosen, O., Manor, R., Chung, J. S., Zmora, N., Glazer, L., Aflalo, D. E.,
Weil, S., Tamone, L. S. and Sagi, A. 2012. Stimulation of molt by RNA interference of the
molt-inhibiting hormone in the crayfish Cherax quadricarinatus. General and Comparative
Endocrinology, 178(2):227-236.
3. Glazer L., Sagi A. 2012. On the involvement of proteins in the assembly of crayfish
gastroliths extracellular matrix. Invertebrate Reproduction Development, 56(1):57-65.
4. Akiva-Tal A., Kababya S., Balazs Y.S., Glazer L., Berman A., Sagi A., and Schmidt A.
2011. Bioavailable calcium stabilization: the in situ molecular NMR picture of biomineralized
amorphous CaCO3. Proceedings of the National Acadamy of Science USA,
108(36):14763-14768.
5. Glazer L., Shechter, A., Tom, M., Yudkovski, Y., Aflalo, A.D., Pamuru, R.R., Khalaila, I.,
Bentov, S., Berman, A., and Sagi, A. 2010. A protein involved in the assembly of an
extracellular calcium storage matrix. Journal of Biological Chemistry, 285(17): 1283112839.
6. Bentov, S., Weil, S., Glazer, L., Sagi, A and Berman, A. 2010. Stabilization of amorphous
calcium carbonate by phosphate rich organic matrix and by single phosphoamino acids.
Journal of Structural Biology, 171(2):207-15.
2
Lilah Glazer
7. Yudkovski, Y., Glazer, L.*, Shechter, A., Reinhardt, R., Chalifa-Caspi, V., Sagi, A., Tom, M.
2010. Multi-transcript expression patterns in the gastrolith disk and the hypodermis of the
crayfish Cherax quadricarinatus at premolt. Comparative Biochemistry and Physiology Part
D Genomics and Proteomics, 5:171-177. *Equal contribution.
8. Ventura T., Manor R., Aflalo E.D., Weil S., Raviv S., Glazer L., Sagi A. 2009. Temporal
silencing of an androgenic-gland-specific insulin-like gene affecting phenotypic gender
differences and spermatogenesis. Endocrinology, 150(3): 1278-1286.
9. Shechter A., Glazer, L., Cheled, S., Mor, E., Weil, S., Berman A., Bentov, S., Aflalo, E.D.,
Khalaila, I., and Sagi A. 2008. A gastrolith protein serving a dual role in the formation of
extracellular matrix containing an amorphous mineral. Proceedings of the National
Acadamy of Science USA, 105(20):7129-7134.
10. Manor, R., Weil, S., Oren, S., Glazer, L., Aflalo, E.D., Ventura, T., Chalifa-Caspi, V.,
Lapidot, M., and Sagi A. 2007. Insulin and gender: an insulin-like gene expressed
exclusively in the androgenic gland of the male crayfish. General and Comparative
Endocrinology, 150(2):326-336.
Presentations at Scientific Meetings
1. Glazer L, Neelakanteswar A and Hahn ME. 2014. Delayed effects of embryonic exposure
to low levels of PCB-126 on adult zebrafish behavior. The fourth international summit of
Prenatal Programming and Toxicity, Boston, MA, U.S.A.
2. Glazer L, Weil S, Mittelman B, Roth Z, Khalaila I, Tom M and Sagi A. 2012. Novel moltrelated hemocyanin family proteins from extracellular matrix of crayfish gastroliths.
Crustacean Society Summer Meeting and 10th Colloquium Crustacea Decapoda
Mediterranea, Athens, Greece.
3. Tynyakov Samra, J, Ben-Tov, S, Glazer, L, Weil, S, Berman, A. and Sagi, A. 2012. Proteins
of the mandible molar tooth in the red claw crayfish Cherax quadricarinatus. Crustacean
Society Summer Meeting and 10th Colloquium Crustacea Decapoda Mediterranea,
Athens, Greece.
4. Glazer L, Weil S, Mittelman B, Roth Z, Khalaila I, Tom M and Sagi A. 2012. Novel moltrelated hemocyanin family proteins from extracellular matrix of crustacean gastroliths. The
Society for Integrative and Comparative Biology, Charelston, SC, U.S.A.
5. Glazer L, Berman A and Sagi A. 2010. Molt-related calcium deposition in crustaceans –
relevant proteins. 9th International Marine Biotechnology Conference, Qingdao, China.
6. Glazer L, Gafni O, Weil S, Berman A and Sagi A. 2010. Chitin associated extra-cellular
proteins in the gastrolith of molting crayfish. International Society of Invertebrate
Reproduction and Development, Prague, Czech republic.
3
Lilah Glazer
7. Gafni O, Glazer L, Weil S, Berman A and Sagi A. 2010. Proteins of the bioavailable
temporary calcium storage organ in the crayfish Cherax quadricarinatus. The 11th Dan
Popper Symposium, Eilat, Israel.
8. Akiva A, Glazer L, Berman A, Sagi A, Kababya S, and Schmidt A. 2010. Understanding
nanostructure stabilization of ACC: A solid state NMR study of intact gastroliths from the
blue lobster. The Israel Chemical Society, Tel-Aviv, Israel.
9. Glazer L, Shechter A, Bentov S, Weil S, Sagi A, Berman A. 2009. Novel molt-related
proteins with possible roles in crayfish calcium storage deposits. The Israel Chemical
Society, Tel-Aviv, Israel.
10. Glazer L, Shechter A, Berman A, Weil S, Aflalo E.D, Yudkovski Y, Tom M and Sagi A.
2009. A novel molt-related protein with a possible role in the formation of crayfish calcium
storage deposits. The Society for Integrative and Comparative Biology, Boston, MA, U.S.A.
11. Glazer L, Shechter A, Bentov S, Berman A, Weil S, Pamuru R.R, Yudkovski Y, Tom M and
Sagi A. 2008. The gastrolith formation - a quest for molt related genes, their encoded
proteins and their functionalities. 9th Colloquium Crustacea Decapoda Mediterranea,
Torino, Italy.
12. Shechter A, Mor E, Raviv S, Glazer L, Ramachandra R.P and Sagi, A. 2007. Silencing of
an ecdysone-responsive gene during crayfish molt cycle. 11th International Congress on
Invertebrate Reproduction and Development, Panama City, Panama.
13. Manor R, Weil S, Oren S, Glazer L, Aflalo E.D, Ventura T, Chalifa-Caspi V, Lapidot M and
Sagi A. 2007. Identification and characterization of an insulin-like gene expressed
exclusively in the androgenic gland of the male crayfish. 8th International Marine
Biotechnology Conference, Eilat, Israel.
4
Lilah Glazer
Research interests
Human and animal exposures to anthropogenic environmental contaminants have been
documented worldwide and are a cause for concern for both human health and wildlife
conservation. Over the past several years there has been increased recognition that the earlylife environment can strongly influence the trajectory of developmental pathways, and that
perturbations at critical stages of development can have persistent or delayed functional
consequences in later life stages. The use of animal models provides experimental platforms
for more thorough evaluation of physiological and behavioral phenotypes and for mechanistic
studies, as well as for the study of chemical-specific congeners in order to determine their
individual roles in mediating the observed outcomes. In addition, animal models facilitate the
continuation of studies from early life stages through adulthood.
In the long term, I am interested in studying the early and later-life consequences of
developmental exposure to anthropogenic environmental contaminants.
For my research, I am using zebrafish as vertebrate model organisms. Zebrafish are
excellent tools for studying later life effects of embryonic exposure for several reasons; their
short generation time is ideal for full embryo-to-adult experiments in relevant time-scales, their
ex utero development and transparent embryos allow for easy evaluation of exposure levels
that do not cause immediate overt effects, their easy maintenance and breeding and high
fecundity allow high throughput experimentation with many biological replicates.
Currently I am focusing on the delayed neurodevelopmental outcomes of embryonic
exposure to the dioxin-like persistent organic pollutant 3,3’,4,4’,5-pentachlorobiphenyl (PCB126), which causes toxicity through the aryl hydrocarbon receptor. At this point I have results
from larval and adult behavior trials showing that embryonic exposure to a low level of PCB126, which does not cause morphological defects or behavioral changes at the larval stages,
does lead to a measurable reduction in anxiety-related behavioral at the adult stage.
My future aims for this study include search for changes in the expression of genes related
to neurodevelopment and neurobehavior, at the time of exposure and at adulthood, that were
caused by the exposure to PCB-126, using RNA extracted from toxin-exposed and vehicleexposed embryos at several developmental time points, as well as RNA extracted from brains
that were dissected from adults used in the behavior assays. In addition, I will search for
histological evidence in juvenile and adult fish, at the RNA and protein levels, of differences in
specific neurological pathways that may be involved the observed phenotypes. Also, I am
conducting additional adult behavior assays in order to look for other behavioral phenotypes,
and for adding robustness to the anxiety-related phenotype already shown.
I believe that this research will provide important novel information on the long-term
consequences of developmental exposure to dioxins, and may also help to determine the
safety levels of environmental exposure to these compounds.
In the long term, I would like to expand my research to include environmentally relevant
toxicant mixtures, and perhaps other stress factors.
5
Lilah Glazer