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Dormancy of cells and organisms strategies for survival and preservation NEST - # 012674 2005-2008 Acronym: Sleeping Beauty Partners • Esther Lubzens (NIO) Ora Hadas, Assaf Sukenik (KLL) -IOLR – P1 • Joan Cerdà, IRTA, Barcelona, Spain – P2 • Stefan Hohmann, Goteborg University, Sweden – P3 • Melody Clark and Roger Worland, BAS, Cambridge, UK – P4 • Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – P5 • Jens Nielson, Danish Technical University, Lyngby, Denmark - P6 Objectives Learning from nature to develop new concepts for cell preservation based on mechanisms with which model organisms tolerate desiccation and/or remain metabolically inactive for long periods Goals • Reveal strategies employed by different organisms that facilitate their long term survival in a dormant form • Widening the basis of our knowledge on the molecular details of strategies employed by 5 model organisms • Resolve whether these strategies can be used to develop preservation (“ reversible dormancy ”) approaches at ambient temperatures for different cells and organisms * Emphasis on understanding processes that control the establishment and maintenance of dormant stages and the exit from dormancy Specific goals WP 1 & 2 Discover the genes, proteins and compounds that are associated with the formation of dormant forms and exit from it in: Cyanobacteria Yeast Rotifers WP 3. Discerning the processes associated with desiccation as a survival strategy by global gene expression, proteome and metabolome profiling Arctic springtail killifish embryos Specific Goals - Continued WP 4. Confirming the role of specific genes in tolerance to desiccation or dormancy by genetic analyses and genetic genetic engineering experiments Yeast Killifish Rotifers (genetically selected strains) WP 5. Discovering common and divergent strategies through synthesis of the genomic, proteomic and metabolomic data of model organisms and of data from other organisms, including plants – in silico models Specific Goals - Continued • WP 6. Publicity to stir and encourage new research direction Scientific publications, scientific meetings Workshop Book • Tools: High through approaches; genomics, proteomics, metabolomics and bioinformatics Partners • Ora Hadas, Assaf Sukenik (IOLR)- Cyanobacteria • Joan Cerdà, IRTA, Barcelona, Spain - Killifish • Stefan Hohmann, Goteborg University, Sweden - yeast • Melody Clark & Roger Worland, BAS, UK – Arctic sprintail • Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – Genomic and proteomic platform • Jens Nielson, Danish Technical University, Lyngby, Denmark metabolomic platform WP Number Title Leading partner & Starting Participating month partners WP0 Management Esther Lubzens WP1 Establishment of dormant stages Esther Lubzens & M0 Ora Hadas- P3, P5, P6 WP2 Arousal from dormancy Stefan Hohmann – P1, P5, P6 M0 WP3 Desiccation and revival Joan Cerdà – P4, P5, P6 M0 WP4 Engineering /genetic testing Joan Cerdà – P1, P3, P5, P6 M15 WP5 Common and divergent pathways Stefan Hohmann & Melody Clark – P1, P2, P5, P6 M0 WP6 Dissemination Esther Lubzens –P2, M12 P3, P4, P5, P6 M0 Pert Chart for SLEEPING BEAUTY WP1 Dormant stages P1, P3, P5 WP2 Germination and arousal from dormant stages P1, P3, P5 WP3 Resistance to desiccation P2, P4, P5 WP1 Dormant stages P1, P3, P5, P6 WP2 Germination and arousal from dormant stages WP4 P1, P3, P5, P6 Engineering /genetic testing strategies WP3 P1, P2, P3 Resistance to desiccation P2, P4, P5, P6 WP5 Identification of common pathways for dormant stages, revival/germination and resistance to desiccation P1-P6 WP6 Participation in scientific meetings, organization of a workshop and publication of a book on dormancy and resistance to dessication P1-P6 Towards novel concepts and methods for cell preservation