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Molecular Genetics Evolution Labs 6-8 100 100 100 100 100 200 200 200 200 200 300 300 300 300 300 400 400 400 400 400 500 500 500 500 500 List the pyrimidines and purines and their characteristics. pyrimidines are thymine and cytosine [single ringed] Purines are adenine and guanine [double ring] In what direction is DNA read? Replicated? it is read 3’ to 5’ and replicates 5’ to 3’ What is the homeobox? 180 nucleotide sequence within homeotic gene encoding part of a protein that binds to DNA of genes and regulates the protein. Which part of DNA is kept after splicing? Exons What is ubiquitin? it is a molecule that attaches to a selected protein to be degraded by a proteasome. The total aggregate of genes in a population at any one time www.accessexcellence.org What is a Gene Pool? The 5 causes of this are genetic drift, gene flow, mutation, nonrandom mating, and natural selection www.pbs.org What is microevolution? This occurs when gene flow is interrupted when a population is divided into geographically isolate subpopulations evolution.berkeley.edu What is Allopatric Speciation? The quest for the simplest explanation for an observed phenomenon What is Parsimony? The same skeletal elements which are anatomical signs of evolution Homologous Structures What is the purpose of the GFP in bacterial transformation? To identify successful transformation and create a “glowing” protein to be expressed as a result of plasmid uptake How does the DNA move through the gel plates and how are they represented? The longer DNA fragments remain at the negative end of the plate because they are too large to move further down the plate. The smaller DNA fragments move more freely towards the positive end of the plate because they contain less base pairs. what are the five conditions needed to maintain Hardy Weinberg equilibrium? No genetic drift No gene flow Large population No mutation Random mating what are the two Hardy Weinberg equations and what do the p and the q represent? P+q=1 shows the allele frequencies p2+2pq+q2 shows the genotypic frequencies P is the dominant Q is the recessive An investigator observes that when pure breeding, long-wing Drosophila are mated with pure breeding, short wing flies, the F1 offspring have an intermediate wing length. When several intermediate wing length flies are allowed to interbreed the following results are obtained: 230 long wings 510 intermediate length wings 260 short wings Determine the mode of inheritance and calculate the Chi-Square to test your hypothesis. Pheno #O #E (O-E) (O-E)^2 (oe)^2/e Long 230 250 20 400 1.6 Int 510 500 10 100 0.2 Short 260 250 10 100 0.4 Autosomal recessive with incomplete dominance Chi-Square = 2.2 2 degrees of freedom at 0.05 probabilty = Accept