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Corn Genetics & Chi- Square Goodness of Fit Test Pedigrees and probability analysis to follow. Outline Highlight some key points in syllabus Corn color and collection of data (phenotypes) Chi-Square Analysis Corn genetics Lab Application of Chi-Square Analysis The color pattern in corn is due to multiple genes Are variations in number of observed phenotypes due to chance alone? Objectives Collect data from a population of corn and then perform Chi-Square Interpret your findings Variation of colors—Why? Corn Kernel Color Color of aleurone masks endosperm color Only with colorless aleurone can endosperm color be determined Determined by several genes Refer to genotypes in lab manual! Gene interaction can occur: Epistasis is a form of gene interaction in which one gene masks the phenotypic expression of another. Today there are: 3 crosses of corn F1 X F1 Multiple alleles are tested for expression in F2 generation Note color differneces Cross C Cross D (darker color is purple) Cross F (call purple, may look red!) Genetic information regarding the corn color patterns are provided in your lab manual. Cross C F1 phenotype F1 X F1 genotypes Yellow YYC’CRr X YYC’CRr Cross D F1 phenotype F1 X F1 genotypes Purple yyPpRr X yyPpRr Cross F F1 phenotype F1 X F1 genotypes Purple yyCcRr X yyCcRr Chi Square Statistical “Goodness of Fit Test” Why do we use it? if. probability of observed event is due to chance alone. To find out determine An example of how to calculate a Chi Square Application of Chi Square Probability Problems Review of some probability problems