* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project
Download Cells & Cell Organelles
Gene regulatory network wikipedia , lookup
Membrane potential wikipedia , lookup
Cell culture wikipedia , lookup
Signal transduction wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Cell-penetrating peptide wikipedia , lookup
Cell membrane wikipedia , lookup
Electrophysiology wikipedia , lookup
Hyper – high Hypo – low Iso - same Lesson 9: Homeostasis and Transport 2006- Demo: Limp Salad Homeostasis Maintaining a balance of conditions within the cell (or organism). • maintaining pH (cells need pH of 6.8-7.2) pH Scale Acid Neutral Base 1 2 3 4 5 6 7 8 9 10 11 12 13 14 most cells QuickLab: pH paper Homeostasis Maintaining a balance of conditions within the cell (or organism). • maintaining temperatures (humans 98.6oF) – shiver/sweat • metabolism = sum total of the body’s chemical activities High metabolism uses energy FAST Low metabolism uses energy SLOWLY Homeostasis Maintaining a balance of conditions within the cell (or organism). • maintaining temperatures (humans 98.6oF) shiver/sweat Temperature increases Exercising Temperature decreases Triggers sweating Evaporative cooling Homeostasis Maintaining other balances: Nutrients coming in Incoming: Nutrients Water Oxygen Salts (Na+) Energy used up Outgoing: Energy Water Carbon Dioxide Products (proteins) Cell Homeostasis (getting stuff in and out) Cells need an inside & an outside… separate cell from its environment cell membrane is the boundary IN OUT food - sugars - proteins - fats salts O2 H2O waste - ammonia - salts - CO2 - H2O products - proteins Cell needs materials in & products or waste out SuperQuick Activity: Colored water into colored water. Building a membrane How do you build a barrier that keeps the watery contents of the cell separate from the watery environment? FATS LIPIDS Remember: oil & water don’t mix!! What substance do you know that doesn’t mix with water? Lipids of cell membrane Membrane is made of special kind of lipid phospholipids “split personality” “attracted to water” phospholipid bilayer phosphate inside cell lipid outside cell QuickLab: Phospholipid people Membrane is a double layer “repelled by water” Semi-permeable membrane Cell membrane controls what gets in or out Need to allow some materials — but not all — to pass through the membrane semi-permeable (semi – partly) only some materials can get in or out So what needs to get across the membrane? sugar lipids aa O2 H 2O salt waste Crossing the cell membrane What molecules can get through the cell membrane without doors or help? fats and oils can pass directly through inside cell waste outside cell lipid salt sugar aa H 2O but… what about other stuff? Cell membrane channels Need to make “doors” through membrane protein channels allow substances in & out specific channels allow specific material in & out H2O channel, salt channel, sugar channel, etc. inside cell waste salt H 2O aa sugar outside cell How do you build a semi-permeable cell membrane? Channels are made of proteins proteins both “like” water & “like” lipids bi-lipid membrane protein channels in bi-lipid membrane Protein channels Proteins act as doors in the membrane channels to move specific molecules through cell membrane Concentration gradient HIGH LOW Movement through the channel Why do molecules move through the membrane if you give them a channel? HIGH ? LOW ? Molecules move from high to low Diffusion (substances move) move from HIGH to LOW concentration across the concentration gradient. Concentration gradient Membrane High Low Diffusion Move from HIGH to LOW concentration passive transport no energy needed QuickActivity: Diffusion of food coloring across a dish. diffusion Simple Diffusion Move from HIGH to LOW fat inside cell LOW fat fat fat fat fat Which way will fat move? HIGH outside cell fat fat fat fat fat fat fat fat Facilitated Diffusion Move from HIGH to LOW through a channel sugar sugar sugar sugar inside cell sugar sugar LOW Which way will sugar move? HIGH outside cell sugar sugar sugar sugar sugar sugar sugar Diffusion Move from HIGH to LOW concentration directly through membrane simple diffusion no energy needed moves through a protein channel facilitated diffusion (with help) no energy needed HIGH LOW Simple vs. facilitated diffusion simple diffusion inside cell lipid facilitated diffusion LOW inside cell H 2O protein channel outside cell outside cell HIGH H 2O Active transport Cells may need to move molecules against concentration gradient need to pump “uphill” from LOW to HIGH using energy protein pump Requires ATP Notice the direction of movement and the concentrations! Low ATP High Let’s Review… simple diffusion facilitated diffusion active transport ATP Osmosis Movement of Water Across Cell Membrane Osmosis Water is so important that we discuss it separately Osmosis diffusion of water from high concentration of WATER to low concentration of water across a semi-permeable membrane Osmosis diffusion of water from high concentration of WATER to low concentration of water across a semi-permeable membrane High Low Virtual Lab 4: Osmosis through a Selectively Permeable Membrane Keeping water balance (Homeostasis) Cell survival depends on balancing water uptake & water loss freshwater balanced saltwater Virtual Lab 5: Onion Cell Plasmolysis Keeping right amount of water in cell Freshwater a cell in fresh water high concentration of water around cell cell gains water example: Paramecium problem: cells gain water, swell & can burst water continually enters Paramecium cell solution: contractile vacuole pumps water out of cell Hypotonic Controlling water Contractile vacuole in Paramecium Keeping right amount of water in cell Saltwater a cell in salt water low concentration of water around cell cell loses water example: shellfish problem: cell loses water plasmolysis in plants shrinking cell solution: take up water Hypertonic Keeping right amount of water in cell Balanced conditions no difference in concentration of water between cell & environment cell in equilibrium example: blood problem: none water flows across membrane equally, in both directions volume of cell doesn’t change Isotonic BLOOD! Take your time to answer each question. Make sure you know what the question is asking. TEST TIPS! Learning through Osmosis?