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Transcript
Human rhinovirus 14 3C protease Data Sheet Creation Date 14-­‐October-­‐2015 Revision Date 14-­‐February-­‐2016 IDENTIFICATION Product Name Human rhinovirus 14 3C protease Product Number Lot Number Gene/Alias Symbol DESCRIPTION Source Modification Predicted Molecular Mass Extinction Coefficient SPECIFICATIONS Purity Endotoxin Formulations SHIPPING AND STORAGE Shipping Lead Time Storage BACKGROUND ESI-­‐H3C-­‐G 151203-­‐001-­‐50G HRV 3C protease E. coli derived N-­‐terminal his-­‐tag 21,819 g/mol −1
−1
5120 M cm > 95% based on SDS-­‐PAGE < 0.01 EU/μg 2 μg/μl in 10 mM Tris, pH 7.0, 125 mM NaCl, 0.5mM EDTA, 2.5 mM β-­‐mercaptoethanol, 50% glycerol Ice Pack FedEx carrier, Domestic & International: within 1-­‐2 weeks delivery, overnight may be available Store at -­‐20°C Description HRV 3C Protease is a fusion protein of glutathione S-­‐transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-­‐Phe-­‐Gln/Gly-­‐Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well. Since the protease is fused to GST, it can be removed from cleavage reactions using Glutathione Sepharose™ 4B. References 1. Walker, P.A. et al., BIO/TECHNOLOGY 12, 601 (1994). 2. Cordingly, M.G. et al., J. Biol. Chem. 265, 9062 (1990). This product is for Research Use Only. Not for diagnostic, human or veterinary use. Optimal conditions of its use should be determined by end users. Disclaimer: The information provided on this Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with another material or in any process, unless specified in the text Exeligen Scientific, Inc. 2351 Faraday Ave Carlsbad, CA 92008|C3767862|[email protected]