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Metabolic Engineering X- Poster Presentation Schedule Poster Group 1 (Sunday, June 15 and Monday, June 16) Poster Number Abstract Title 1 2 3 4 5 6 7 8 9 10 11 The Genetic and Metabolic Regulation of Rhamnolipids Biosynthesis in Pseudomonas Aeruginosa Reveals New Engineering Strategies for Heterologous Expression Isotopically Nonstationary 13C Flux Analysis of Isobutyraldehyde Production in Synechococcus Elongatus Characterization of LDH Genes for L-Lactic Acid Production in Pichia Pastoris Identification of Key Metabolite Concentrations and Enzyme Saturations Determining the Physiological States of Glucose-Fed E. coli for the Production of 1,4-Butanediol New Advances in 13C Metabolic Flux Analysis: Complete-MFA, Co-Culture MFA and Dynamic MFA Parallel Labeling Experiments: A Novel Approach for Validating Metabolic Network Models Synthetic Microbial Metabolism Refactoring for the Production of a Chemical Synthon, 2,4-Dihydroxybutyric Acid Implementation of a Disassociated Fatty Acid Synthase System (FAS type II) in Saccharomyces Cerevisiae for Fatty Acid and Wax Ester Production Production of 4-Hydroxybutyric Acid By Metabolically Engineered Mannheimia Succiniciproducens and Its Conversion to Gamma-Butyrolactone By Acid Treatment Challenges in Reverse Engineering of Industrial Fermentation Strains Metabolic Engineering for Ricinoleic Acid Production in Y. Lipolytica Fatty Acid Overproduction in a Genetically Engineered Puryvate 12 Decarboxylase-Negative Strain of Saccharomyces Cerevisiae Isolation, Characterization and Metabolic Engineering of a 13 Thermophilic Bacillus for Green Chemical Production Controlled Protein Degradation for Development of Metabolite 14 Valves Flexible and User Friendly Tools for the Incorporation of 15 Fluxomics Data into Metabolic Models Unraveling the Inhibitory Effects of Acetate on Ethanol 16 Production in Cen.PK Understanding and Optimizing Free Fatty Acid Production in 17 Synechocystis Sp. PCC 6803 18 Novel Acetyl-CoA Transfer Route in Saccharomyces Cerevisiae 19 Microbial Production of Short-Chain Alkanes Last Name First Name AbdelMawgoud Ahmad M. Adebiyi Adeola Almeida Pollyne B. A. Andreozzi Stefano Antoniewicz Maciek R. Au Jennifer Auriol Clément Azevedo Flávio Bang Junho Benjamin Kirsten R. Beopoulos Athanasios Bergman Alexandra Bosma Elleke F. Brockman Irene Carreira Rafael Chakrabarti Anirikh Cheah Yi Ern Chen Choi Yun So Young High-Throughput Screening System and Its Biotechnological 20 Applications Overcoming Inefficient Cellobiose Fermentation By Cellobiose 21 Phosphorylase in the Presence of Xylose Production of Phenol from Glucose in Escherichia coli through 22 Metabolic Engineering Approach Towards Synthetic Phototrophy: Engineering Proton-Pumping 23 Rhodopsins into E. coli Engineering Anaerobic Amino Acid Production in 24 Saccharomyces Cerevisiae: Alanine As Case of Study Cytosolic Acetyl-CoA Platform in Yeast for Biochemicals 25 Production 26 Toward a Biosynthetic Route to Sclareol and Amber Odorants 27 Advanced Production of Faee in a S. Cerevisiae Cell-Factory Metabolic Engineering of Cyclic Triterpenoid Production in 28 Saccharomyces Cerevisiae Development & Exploitation of Gene Tools for Metabolic 29 Engineering in Saccharolytic Clostridia 30 Novel Methods to Investigate Solvent Toxicity in Bacteria Targeted Proteomics Enabled Metabolic Engineering of 31 Clostridium Cellulolyticum for n-Butanol Production Isobutanol Production By an Industrial Saccharomyces 32 Cerevisiae Strain Metabolic Engineering of Photorespiratory Bypass Pathways to 33 Enhance Novel Biofuel Production in Transgenic Plants Metabolic Activities and Their Control at the Mitochondria34 Cytosol Interface in CHO Cells General and Specific Stress Responses Towards Short EvenChain Alcohols in Lactic Acid Bacteria Provide Clues for 35 Improving Second Generation Biorefineries Isotopically Nonstationary 13C Metabolic Flux Analysis of 36 Arabidopsis thaliana Rosettes at Altered Light Conditions Biotechnical Production of Ethylene in S. Cerevisiae - Insights from Metabolic Modeling, Cultivation Studies and Enzyme 37 Engineering Microbial Production of Cis,Cis-Muconic Acid By Klebsiella 38 Pneumoniae. Effects of Pyruvate Formate Lyase Inactivation in Klebsiella 39 Pneumoniae and Its Application to Diol Production Bio-Hydrogen Production By Continuous Culture of 40 Hyperthermophilic Archaeon from Carbon Monoxide Prediction and Design of Novel Metabolic Pathways for the 41 Production of Desired Chemicals Choi Jong Hyun Chomvong Kulika Chung Hannah Claassens Nico J. Cueto-Rojas Hugo Federico Dai Zongjie Daviet de Jong Laurent Bouke Wim Ebert Birgitta E. Ehsaan Fletcher Muhammad Eugene Gaida Stefan M. Generoso Wesley Cardoso Goklany Sheba Heinzle Elmar Hviid Anne-Mette Jazmin Lara J. Johansson Nina Jung Hwi-Min Jung Moo-Young Kim Tae Wan Kim Dong In 42 43 44 45 46 47 48 49 50 Integration of Transcriptomic Data in Genome-Scale Metabolic Models Predicts in Vitro Intracellular Central Carbon Metabolic Fluxes with High Correlation in Escherichia coli and Saccharomyces Cerevisiae Model-Driven Metabolic Engineering of Escherichia coli for Improving Conversion of Lignocellulose-Derived Sugars to Ethanol Yeast Cell Factories for Production of Biobutanol Engineering of a Stable, Syntrophic Microbial Coculture for Enhanced H2 Production Redirecting Photosynthetic Reducing Power into Light-Driven Biosynthesis of Bioactive Natural Compounds Biosynthesis of 2-Hydroxyacid Containing Polyhydroxyalkanoates in Metabolically Engineered Ralstonia Eutropha Biosynthesis of Polyhydroxyalkanoates in Recombinant Ralstonia Eutropha Engineered to Utilize Sucrose As a Carbon Source Comparative Cross-Strain Analysis of Stress Resistance Mechanisms Revealed By Transposon Insertion Sequencing Construction of a Efficient Xylose Metabolic Pathway in Saccharomyces Cerevisiae for Ethanol Production 51 Development of a Yeast Cell Factory for Resveratrol Production Modelling Population Dynamics of Pseudomonas Putida KT2440 52 Under Various Growth Conditions Carbon Flux-Associated Redox Rebalancing By Static and 53 Dynamic Control Enhanced Utilization of Non-Favored Sugars from Marine 54 Biomass By Re-Designed Escherichia coli Comprehensive Study of Metabolic Flux Rewiring in E. coli 55 Knockout Strains Metabolic Engineering of Pseudomonas Putida KT2440 for the Production of Compounds Derived from the Shikimic Acid 56 Pathway Metabolic Engineering of Yeast for Commercial Production of 57 Succinic Acid Vitamin Analogs As Antiinfectives: Occurrence, Mode of Action, 58 Metabolism and Production Genome-Scale Strain Designs Based on Regulatory Minimal Cut 59 Sets A Bayesian Design of Experiments for Ensemble Modelling of 60 Metabolic Networks Developing an Integrated Systems and Synthetic Biology 61 Platform for Gas Fermenting Acetogens Rational Genome Engineering with Genetically Encoded 62 Biosensors at Single-Cell Scale Kim Min Kyung Kim Krivoruchko Joonhoon Anastasia LaSarre Lassen Breah Lærke Marie Münter Lee Sang Yup Lee Sang Yup Lennen Rebecca M. Li Yunjie Li Mingji Lieder Sarah Lim Jae Hyung Lim Hyun Gyu Long Christopher P. Lorenz Silvia Los Alrik Mack Matthias Mahadevan Radhakrishnan Manesso Erica Marcellin Esteban Marienhagen Jan An Integrated Multi-Omics and Computational Characterization of Seven Unique Escherichia coli Production Strains CommonlyMonk 63 Used in Industrial Biotechnology Computationally Guided Characterization of Carboxylic Acid Moura 64 Reductases for Expanding Aldehyde Bioproduction Mueller 65 K-Optforce: Strain Design Using Kinetic Information 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 Rational Design & Optimization of a Synthetic Entner-Doudoroff Pathway for Improved & Controllable NADPH Regeneration Strategies for Improving Renewable Phenol Biosynthesis in Engineered Escherichia coli Synthesis of Nylon 6,5 from Biologically Prepared 5AVA By Metabolically Engineered Escherichia coli Metabolic Flux Analysis of Isopropyl Alcohol-Producing Escherichia coli The D494G Point Mutation in the Bifunctional Alcohol and Aldehyde Dehydrogenase (adhE) of Clostridium Thermocellum Leads to Improved Ethanol Production Identifying Bottlenecks in Engineering Efficient Cellobiose Metabolism (Evidence for putative promoters within operon and TCA cycle imbalance) Building Metabolic Engineering Tools to Better Understanding Product Production from Microbial Sources: Using the Cyanobacterium Synechocystis Sp. PCC 6803 for Astaxanthin Production Steering Prokaryotic Gene Expression Using Engineered Riboswitches Sensor-Selector Strategy for Directed Evolution of Biosynthetic Pathways Rapid Evaluation of Itaconic Acid Production Strategies in Saccharomyces Cerevisiae Production of Aromatic Compounds in E. coli Strains Lacking Interconversion of PEP and Pyr When Glucose and Acetate Are Coutilized Metabolic Transistor Strategy for Controlling Electron Transfer Chain in Escherichia coli Ubiquinone Accumulation Improves Osmotic-Stress Tolerance in Escherichia coli Transport and Metabolism of Fumaric Acid in Saccharomyces Cerevisiae Implementing the Formose Pathway for Conversion of Electricity and CO2 to Biofuel Precursors Via Formate in Escherichia coli Engineering the Valine Assimilation Pathway to Produce Biochemicals and Fuels in S. Cerevisiae Systems Metabolic Engineering of Escherichia coli for the Production of Fumaric Acid Jonathan M. Matthew Thomas Ng Chiam-Yu Nielsen David R. Oh Young Hoon Okahashi Nobuyuki Olson Daniel Parisutham Vinuselvi Peebles Christie A.M. Peters Gert Raman Srivatsan Roubos Hans Sabido Andrea San Ka-yiu Sevin Daniel C. Shah Mihir Smith Amanda Lee Solomon Kevin Song Chanwoo 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 Engineering the Glycolytic Pathway of E. coli K12 Mutants By Gene Deletions and Introduction of a Fructose 6-Phosphate Aldolase The Potential of Lactic Acid Bacteria As Microbial Factory for Pentanol Isomer Production Engineering Saccharomyces Cerevisiae for the Production of Hexadecanol and Octadecanol L-Methionine Production with Recombinant E. Coli Engineering of Artificial Enzyme Complexes Mediated By Heterospecific Coiled-Coil Zippers or Synthetic Protein-Protein Interaction Domains in Saccharomyces Cerevisiae Driven By Demand Metabolic Engineering - Recombinant Rhamnolipid Synthesis in Pseudomonas Putida As an Example SMET: Systematic Multiple Enzyme Targeting for Rational Design of Optimal Strains Mathematical Modelling of Apoptosis for GS-NS0 Cell Culture Secreting Monoclonal Antibody: Linking Gene to Growth, Metabolism and Metabolic Stress Strong Reduction of Acetate Overflow in Escherichia coli By Systems Metabolic Engineering Cytosolic Acetyl-CoA Synthesis By Pyruvate-Formate Lyase in Yeast Itaconic Acid Production in Escherichia coli By Overexpression of Citrate Synthase, Aconitase, and Cis -Aconitate Decarboxylase Metabolic Engineering of Klebsiella Pneumoniae for 1-Butanol Production By Using Crude Glycerol Single-Cell Bioreactors Boost Bioprocess Development: New Insights into Cellular Metabolism Corynebacterium Glutamicum Engineered As a Designer Bug for the Production of Pyruvate and Succinate Systems Metabolic Engineering of Corynebacterium Glutamicum to Overcome the Cellular Toxicity Derived from Cellulosic Hydrolysate Development of Genetic Tools for the Metabolic Engineering of the Thermophilic Acetogen Moorella Thermoacetica Construction of Fast Xylose-Fermenting Yeast Based on Industrial Ethanol-Producing Diploid Saccharomyces Cerevisiae By Rational Design and Adaptive Evolution Bacterial Cell Factory for Production of Scyllo-Inositol, a Potential Therapeutic Agent for Alzheimer's Disease Construction of a Hybrid Pathway for Selectively Removing Nitrogen Atom from Carbazole Protein Design for a De Novo Synthetic Pathway of Microbial Production of 1,3-Propanediol from Sugar Sprenger Georg A. Starlit Karen I. Stuart Takors David Ralf Thomik Thomas Tiso Till Trinh Cong T. Usaku Chonlatep Valgepea Kaspar van Rossum Harmen M. Vuoristo Kiira Wang Miaomiao Wiechert Wolfgang Wieschalka Stefan Woo Han Min Woolston Benjamin Yang Junjie Yoshida Ken-ichi Yu Bo Zeng An-ping 103 104 105 106 107 Systematic Characterization of Protein–Protein Interface for the Development of Artificial Biomachinery for Metabolic Engineering Dynamic Control of Metabolism through Engineering LigandInduced Allosteric Regulation Based on a New Concept of Thermodynamic Cycle of Protein Dynamics Development of the First Scalable Rubbery Polyester Co-Culture Based Modular Engineering for Aromatic and Aromatic-Derived Compounds Production in E. coli A Fast Metabolic Sensor for in V ivo Cytosolic Phosphate Concentration in Saccharomyces C erevisiae Cooperative Co-Culture of Escherichia coli and Saccharomyces 108 Cerevisiae for Overproduction of Paclitaxel Precursors Systematic Engineering of Lipid Metabolism for Fatty-Acid109 Based Biofuel Production Zeng An-Ping Zeng Zhang An-Ping Kechun Zhang Haoran Zhang Jinrui Zhou Kang Zhou Yongjin Poster Group 2 (Tuesday, June 17 and Wednesday, June 18) Poster Number Abstract Title 110 Engineering a Balanced Mevalonate Pathway in E.coli Yeast Mitochondrial Engineering: Targeting the Powerhouse of 111 the Cell for Advanced Biofuel Production Combining Elementary Mode Analysis with a Network Embedded Thermodynamic Approach for Analysis of Microbial 112 Adipic Acid Production Metabolic Reconstruction of Clostridium Acetobutylicum for 113 Enhanced Production of Butyric Acid Development of Next Generation Yeast Strains for Ethanol 114 Production from Lignocellulosic Feedstocks Identifying the Source of Strain-to-Strain Variability in Isoprenoid Production Capacity of E. coli Using a Systems 115 Biology Approach Rational Metabolic Engineering of Baker's Yeast for Production 116 of 3-Hydroxypropionic Acid Understanding and Overcoming Monoterpene Toxicity in Yeast 117 for the Production of Renewable Jet Fuels Molecular Approaches to Improve 1-Butanol Tolerance and 118 Production in Escherichia coli Metabolic Engineering for Production of 5-Aminovalerate and 119 Glutarate Using Escherichia coli Synthetic Regulatory Small RNAs for Genome-Wide Metabolic 120 Engineering Last Name AlonsoGutierrez Jorge Avalos Jose L. Averesch Nils J. H. Bang Junho Boer Viktor Bongers Mareike Borodina Irina Brennan Timothy Bui Le Minh Chae Tong Un Chae Tong Un Analysis of Aerobic-to-Anaerobic and Anaerobic-to-Aerobic 121 Switches in E. coli Using Large-Scale Dynamic Metabolic Models Chakrabarti Direct Fermentation for Isobutene, Butadiene and Propylene Production : A Highway to Renewable Plastics, Synthetic Rubber Chayot 122 and Fuels Biosynthesis of Lactate-Containing Polymers in Metabolically Choi 123 Engineered Escherichia coli 124 125 126 127 128 First Name Production of Native-Sized Spider Dragline Silk Protein through Metabolic Engineering Approach in Escherichia coli Chung Splitting the E. coli Metabolism for the Production of Fructose-6P Derived Chemicals Coussement Engineering S. Cerevisiae Metabolism for Efficient Production of Acetyl-CoA Derived Products Denby Engineering Yeast to Produce Fatty Acid-Derived Fuels and Chemicals d'Espaux Construction of Fast Xylose-Fermenting Yeast Based on Industrial Ethanol-Producing Diploid Saccharomyces Cerevisiae By Rational Design and Adaptive Evolution Diao Anirikh Romain So Young Hannah Pieter Charles Leopold Liuyang Engineering a Functional Deoxyxylulose Phosphate (DXP) 129 Pathway in Saccharomyces Cerevisiae Expression Optimization of Multi-Enzyme Pathways for Xylose 130 Utilization and Chemical Production The Importance of the Lipid Biosynthetic Pathway for 131 Glycolipids Production in Engineered E. coli Cells Determining the Control Circuitry of Redox Metabolism at the 132 Genome-Scale 133 134 135 136 137 138 Implications of the Assumptions on Intracellular Metabolic Operational States in Metabolic Control Analysis Novel Biosensors for Optimizing Yeast Cell Factories 13C Metabolic Flux Analysis of Co-Culture Systems: A Novel Approach Targeted Omics Informed Engineering to Improve C5 Alcohol Production in E. coli A Novel Design of a Translation Coupling-RNA Scaffold System to Improve the Efficiency of Molecular Chaperone on Recombinant Proteins Solubilization 2-Butanol and Butanone Production in Saccharomyces Cerevisiae through the B12 Dependent Dehydratase Pathway Using a Tev-Based Expression System 139 Using Metrxn for Flux Elucidation and Model Reconstruction Use of Transporter Plug-Ins for Enhanced Productivity and Reduced Byproduct Formation of Bioalkanes and Related 140 Compounds Engineering Efficient Xylose Metabolism Using Synthetic 141 Biology Using Protein Scaffolds to Redirect Photosynthetic Reducing 142 Power for Biosynthesis of Natural Products Assessing Metabolic Response to Increased Substrate Loading 143 Rate in Mixed-Culture Fermentation of Waste Water Genetic Engineering to Produce Higher Alcohols in Yeast 144 Saccharomyces cerevisiae Directed Evolution of Terpene Synthases Using HighThroughput Colorimetric Screening Based on Substrate 145 Consumption Dietzel Kevin Dueber John E. Faijes Magda Federowicz Stephen Fengos Florian Geogios David Gebreselassie Nikodimos A. George Kevin W. Geraldi Almando Ghiaci Payam Gopalakrishna n Saratram Grant Chris Hennessy Henriques de Jesus Rosanna Hoelzle Robert D Ishii Jun Iwasaki Miki Enhanced Biofuel Production through Coupled Consumption of Jin 146 Acetic Acid and Cellulosic Sugars By Engineered Yeast Lysate of Engineered Escherichia coli Supports Conversion of Glucose to 2,3-Butanediol with Near-Theoretical Yields and Kay 147 Ultrahigh Productivity Evolution Reveals a Glutathione-Dependent Mechanism of 3Kildegaard 148 Hydroxypropionic Acid Detoxification Maria Yong-Su Jennifer E. Kanchana R. Optimality of Microbial Metabolism with Biosynthetic 149 Heterologous Reactions Deciphering Thermodynamics in Metabolic Networks: A Priority 150 List of Candidates for Metabolomics 151 Malic Acid Production By Aspergillus Oryzae Feasibility Studies of New Strategy for Ultra-High-Throughput Screening (uHTS) of Novel Enzyme By in Vitro Compartmentalization (IVC) Using Microbeads from 152 Metagenomic Resources Replacement of the Saccharomyces Cerevisiae Acetyl-CoA Synthetases By Acetylating Acetaldehyde Dehydrogenase for 153 Cytosolic Acetyl-CoA Synthesis Kim Dong In Kiparissides Knuf Alexandros Christoph Ko Kyong-Cheol Kozak Barbara U. Decorating Bacterial Surfaces By Designer Molecules Advances Kuru 154 the Fundamental Knowledge about Bacterial Growth Metabolic Modulation in Response to Chemical-Induced Signal Lee 155 Transduction in Chlamydomonas Reinhardtii Metabolically Engineered Escherichia coli for Isoprene Liu 156 Biosynthesis Application of a Genetically-Encoded Metabolite Sensor for 157 Single Cell Analysis and Development of Production Strains On the Effects of Phenotype Prediction Methods over Strain 158 Design Algorithms. a Multi-Objective Approach Exploring Bacterial Microcompartments to Establish Orthogonal 159 Metabolism Redemption: Reduced Dimensional Ensemble Modeling and 160 Parameter Estimation Metabolic Engineering of Yeast Central Metabolism for Higher 161 Alcohol Production The Impact of Orthogonal Gene Expression on Heterologous 162 Pathway Productivity Tools to Resolve Compartmentalized Metabolism in 163 Mammalian Cells Identification of the Missing Enzymes for the Heterologous 164 Production of n-Butanol at High Yield Jung-eun Chun-Li Mahr Regina Maia Paulo Mampel Joerg Manesso Erica Matsuda Fumio McArthur George H. Metallo Christian M. Meynial-Salles Isabelle Engineering of Artificial Enzyme Complexes Mediated By Heterospecific Coiled-Coil Zippers or Synthetic Protein-Protein Mignat 165 Interaction Domains in Saccharomyces Cerevisiae Engineering Improved Productivity of 1,4-Butanediol in E. coli – Miskovic 166 a Kinetic Modeling Approach Designing a Nitrogen Fixation Circuit in an Oxygenic Mueller 167 Photosynthetic Organism Effect of CO Transfer on Hydrogen Production By 168 Carboxydotrophic Archeon Thermococcus Onnurineus NA1 Erkin Na Cora Ljubisa Thomas Jeong Geol Streamlining Central Catabolism for à La Carte Activation of 169 Glycolysis 170 Towards a Chassis Organism for Synthetic Biology 171 172 173 174 175 176 Computing Proteome Abundance and Activity with a GenomeScale Model of Metabolism and Gene Expression Deciphering Dynamic Regulation Patterns of CelluloseDegrading Enzymes in Anaerobic Fungi Aspergillus Terreus Isolated from the Brazillian Diversity: Genomic Variability Associated to Lovastatin Production Regulation of Ancillary Reactions Around the Central Carbon Metabolism of E. coli Examining the Complex Transcriptional Response of Perturbing Anthranilate Synthase in the Terpenoid Indole Alkaloid Pathway in Catharanthus Roseus Discovery of Gene Overexpression Targets for Biofuel Product Tolerance in Yeast 177 Microbial Production of Renewable Monoethylene Glycol Improving the Internal Flux Distributions from Genome Scale 178 Metabolic Models of S. Cerevisiae 179 180 181 182 183 The Yeast Pathway Kit: A Method for Rational or Combinatorial Metabolic Pathways Design in Saccharomyces Cerevisiae Efficient Searching and Annotation of Metabolic Networks Using Chemical Similarity Development of a Highly Efficient Gene Delivery System for Syngas Fermenting Clostridia Retooling Glycolysis in Saccharomyces Cerevisiae for More Efficient Isoprenoid Production Systematic Characterization of Intracellular Metabolic States through Flux Directionality Profile Analysis 184 Trans-Regulatory Elements As Tools for Metabolic Engineering Multiplex Amino Acid Metabolism Engineering for Increased 185 Production of L-Ornithine in Yeast Improved N -alkanes Production in Escherichia coli By Spatial 186 Organization of Alkane Biosynthetic Pathway Enzymes Tools for Advancing Genome Engineering on the Protein, 187 Pathway, and Genome Scale Ultra-High-Throughput Screening of Enzyme Libraries with 188 Droplet-Based Microfluidics Nikel Noack Pablo I. Stephan OBrien Edward J. O'Malley Michelle A. Parachin, N.S. Nádia Parisutham Vinuselvi Peebles Christie A.M. Peña Pedro V. Pereira Brian Pereira Rui Pereira Filipa Pertusi Dante Philipps Gabriele Pickens Lauren Pinto Vieira Joana Politz Mark Qin Jiufu G. Rahman Ziaur Reynolds T. Steele Romero Philip 189 Kinetic Reconstruction and Analysis of Sphingolipid Metabolism Savoglidis De Novo Production of Monoterpenoic Acids with Schrader 190 Pseudomonas Putida Georgios Jens A Computationally-Driven Metabolic Engineering Strategy to 191 Increase Cellulose Production in Plants Metabolic Engineering of an Endogenous Pathway for n-Butanol 192 Production in Saccharomyces Cerevisiae Development of an in silico Tool of Novel Metabolic Pathway 193 Designs for Microbial Productions Production of Fatty Acid Derived Biofuels in Saccharomyces 194 Cerevisiae Rapid One-Step Inactivation of Single or Multiple Genes in 195 Escherichia coli Pigment-Based, Low-Cost, Portable Micronutrient Status Tests 196 Using Engineered Bacteria The Role of Trehalose Metabolism in Saccharomyces C erevisiae 197 from a Quantitative Approach Characterization of Anaerobic Central Metabolism to Improve 198 Succinate Production in Enterobacter Aerogenes Expanding Biosynthetic Pathways Based on Thermodynamic 199 Preferences Fatty-Acid Production in Yeast through Reversal of the Beta200 Oxidation Cycle Increased 3-Hydroxypropionic Acid Production from Glycerol Based on the Genome-Scale Metabolic Simulation in 201 Escherichia coli Engineering S. Cerevisiae for Production of Euphorbiaceae 202 Diterpenes 203 204 205 206 207 Improving Dipicolinic Acid Production By Bacillus S ubtilis during Stationary Phase Based on 13c-Metabolic Flux Analysis Construction of E. coli Reporter Strains for the Study of StressAnswer Regulator Proteins Rational Design of Modular Cells for Efficient Combinatorial Biosynthesis of Designer Bioesters Design and Construction of an Artificial Nonmevalonate Operon of Escherichia coli Lactic Acid Production from Xylose By Engineered Saccharomyces Cerevisiae 208 Model Driven Mechanistic Analysis of Adaptive RNAP Mutations Carbon Dioxide Fixation By Calvin-Cycle Enzymes Improves 209 Ethanol Yield in Yeast Rational Engineering of the Methylerythritol Phosphate 210 Pathway through Metabolic Control Analysis Comprehensive Evaluation of Two Genome-Scale Metabolic 211 Network Models of Scheffersomyces Stipitis Senger Ryan S. Shi Shuobo Shirai Tomokazu Siewers Verena Song Chanwoo Styczynski SuarezMendez Mark P. Tajima Yoshinori Tashiro Yohei Teixeira Paulo G. Tokuyama Kento Tong Gary J. Toya Yoshihiro Trachtmann Natalia Trinh Cong T. Tsuge Kenji Turner Timothy L. Utrilla Jose van Maris Antonius J.A. Volke Daniel Wang Jin Camilo A. 212 213 214 215 216 217 218 Genome Scale Metabolic Modeling Reveals New Insights into Biomass Production in the Marine Sponge Amphimedon Queenslandica Quorum-Sensing Linked RNAi for Dynamic Pathway Control in Saccharomyces Cerevisiae Balancing Flux through Secondary Metabolic Pathways in Plant Culture Systems Optimization of Multi-Gene Biological Systems Using HighThroughput DNA Assembly, Sequencing, and Model-Guided Search Strategies Evaluation of Biosynthetic Pathways for Conversion of Natural Gas to Liquid Fuels High-Efficiency Scarless Genetic Modification Method in Escherichia coli without Counterselection Metabolic Engineering of a Biosensor-Based Screening Platform in Yeast Metabolic Engineering for Production of Valuable Chemicals 219 Based on Escherichia coli Strains Designed in silico Watson Jabin Williams Thomas C. Wilson Sarah Woodruff Lauren B. A. Woolston Benjamin Yang Junjie Zhang Jie Zhang Xiaolin